(Circular) (Six-base) MAPSORT of: pFN21ASDB0046.seq Check: 5465 from: 1 to: 10654 pFN21ASDB0046 10654 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2812 2812 Size: 10654 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8464 278 Size: 53 83 186 936 6928 2468 Fragments arranged by size: 6928 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7355 7997 1937 Size: 5418 642 4594 Fragments arranged by size: 5418 4594 642 Acc65I G'GTAC_C Cuts at: 7975 7975 Size: 10654 AclI AA'CG_TT Cuts at: 1563 8781 9154 1563 Size: 7218 373 3063 Fragments arranged by size: 7218 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2140 2472 2538 3099 3252 3498 3999 4272 Size: 508 332 66 561 153 246 501 273 Cuts at: 4272 5877 5913 6328 6684 7077 7667 7844 7854 Size: 1605 36 415 356 393 590 177 10 Cuts at: 7854 8717 10101 10369 1632 Size: 863 1384 268 1917 Fragments arranged by size: 1917 1605 1384 863 590 561 508 501 415 393 356 332 273 268 246 177 153 66 36 10 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10457 AflIII A'CryG_T Cuts at: 1203 2336 2776 3216 9553 10530 1203 Size: 1133 440 440 6337 977 1327 Fragments arranged by size: 6337 1327 1133 977 440 440 AgeI A'CCGG_T Cuts at: 7157 9485 7157 Size: 2328 8326 AhdI GACnn_n'nnGTC Cuts at: 9383 9383 Size: 10654 AleI CACnn'nnGTG Cuts at: 1166 3221 6015 6665 7864 1166 Size: 2055 2794 650 1199 3956 Fragments arranged by size: 3956 2794 2055 1199 650 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3599 1184 Size: 2415 8239 AlwNI CAG_nnn'CTG Cuts at: 2312 2927 4022 4496 4511 4687 5534 7382 7645 Size: 615 1095 474 15 176 847 1848 263 Cuts at: 7645 7727 8397 9969 2312 Size: 82 670 1572 2997 Fragments arranged by size: 2997 1848 1572 1095 847 670 615 474 263 176 82 15 ApaLI G'TGCA_C Cuts at: 8711 9867 8711 Size: 1156 9498 ApoI r'AATT_y Cuts at: 1493 3109 3532 3710 6429 7322 7332 7605 7963 Size: 1616 423 178 2719 893 10 273 358 Cuts at: 7963 8189 8341 10450 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2719 2109 1697 1616 893 423 358 273 226 178 152 10 AseI AT'TA_AT Cuts at: 160 1032 9208 160 Size: 872 8176 1606 Fragments arranged by size: 8176 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10654 AvaI C'yCGr_G Cuts at: 1870 1943 6099 6360 7979 1870 Size: 73 4156 261 1619 4545 Fragments arranged by size: 4545 4156 1619 261 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 6595 6628 6595 Size: 33 10621 BamHI G'GATC_C Cuts at: 5191 7984 8351 5191 Size: 2793 367 7494 Fragments arranged by size: 7494 2793 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4818 7975 9430 618 Size: 325 217 627 3031 3157 1455 1842 Fragments arranged by size: 3157 3031 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 4836 5921 6504 7136 7692 7973 1391 Size: 3445 1085 583 632 556 281 4072 Fragments arranged by size: 4072 3445 1085 632 583 556 281 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2955 3355 4005 4136 4638 5135 5421 Size: 971 1056 400 650 131 502 497 286 Cuts at: 5421 7430 928 Size: 2009 4152 Fragments arranged by size: 4152 2009 1056 971 650 502 497 400 286 131 BbvCI CC'TCA_GC Cuts at: 2373 5721 2373 Size: 3348 7306 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8845 8879 1903 Size: 34 6908 34 3678 Fragments arranged by size: 6908 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3670 3746 8546 9756 3670 Size: 76 4800 1210 4568 Fragments arranged by size: 4800 4568 1210 76 BclI T'GATC_A Cuts at: 1548 1548 Size: 10654 BfrBI ATG'CAT Cuts at: 3478 3478 Size: 10654 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6226 6297 9265 Size: 107 122 71 631 605 4554 71 2968 Cuts at: 9265 136 Size: 1525 Fragments arranged by size: 4554 2968 1525 631 605 122 107 71 71 BglII A'GATC_T Cuts at: 3118 4480 10649 3118 Size: 1362 6169 3123 Fragments arranged by size: 6169 3123 1362 BlpI GC'TnA_GC Cuts at: 4661 8071 4661 Size: 3410 7244 Bme1580I G_kGCm'C Cuts at: 1790 4821 6360 7753 8715 9871 1790 Size: 3031 1539 1393 962 1156 2573 Fragments arranged by size: 3031 2573 1539 1393 1156 962 BmgBI CAC'GTC Cuts at: 1318 3219 4978 1318 Size: 1901 1759 6994 Fragments arranged by size: 6994 1901 1759 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2757 4384 5922 Size: 416 53 83 399 24 1340 1627 1538 Cuts at: 5922 7408 8385 9343 442 Size: 1486 977 958 1753 Fragments arranged by size: 1753 1627 1538 1486 1340 977 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 10654 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4088 4396 4088 Size: 308 10346 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2265 3678 4282 4395 6195 6408 6465 Size: 932 94 1413 604 113 1800 213 57 Cuts at: 6465 6946 7535 7706 9314 1239 Size: 481 589 171 1608 2579 Fragments arranged by size: 2579 1800 1608 1413 932 604 589 481 213 171 113 94 57 Bpu10I CC'TnA_GC Cuts at: 2373 5721 6025 2373 Size: 3348 304 7002 Fragments arranged by size: 7002 3348 304 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3649 5226 5367 5539 7613 8779 9644 9942 3649 Size: 1577 141 172 2074 1166 865 298 4361 Fragments arranged by size: 4361 2074 1577 1166 865 298 172 141 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4916 9317 882 Size: 617 3417 4401 2219 Fragments arranged by size: 4401 3417 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 7951 8445 493 Size: 711 6747 494 2702 Fragments arranged by size: 6747 2702 711 494 BsaBI GATnn'nnATC Cuts at: 10648 10648 Size: 10654 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6152 8461 8843 9478 Size: 53 83 186 936 4619 2309 382 635 Cuts at: 9478 275 Size: 1451 Fragments arranged by size: 4619 2309 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7157 9087 9485 9759 9906 10317 7157 Size: 1930 398 274 147 411 7494 Fragments arranged by size: 7494 1930 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 6674 6704 6923 6953 7113 Size: 30 165 30 5271 30 219 30 160 Cuts at: 7113 7143 7679 7709 1178 Size: 30 536 30 4123 Fragments arranged by size: 5271 4123 536 219 165 160 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2271 3255 4019 5267 5864 5911 5944 6117 Size: 1085 984 764 1248 597 47 33 173 Cuts at: 6117 6179 6466 7331 7610 1186 Size: 62 287 865 279 4230 Fragments arranged by size: 4230 1248 1085 984 865 764 597 287 279 173 62 47 33 BseYI C'CCAG_C Cuts at: 3393 4687 5477 6092 6572 6943 7365 7580 7810 Size: 1294 790 615 480 371 422 215 230 Cuts at: 7810 9857 10353 3393 Size: 2047 496 3694 Fragments arranged by size: 3694 2047 1294 790 615 496 480 422 371 230 215 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2862 6720 7740 1163 Size: 573 1126 3858 1020 4077 Fragments arranged by size: 4077 3858 1126 1020 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8104 8865 9893 1540 Size: 275 186 6103 761 1028 2301 Fragments arranged by size: 6103 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3356 4382 5665 7692 8715 8800 9871 3356 Size: 1026 1283 2027 1023 85 1071 4139 Fragments arranged by size: 4139 2027 1283 1071 1026 1023 85 BsmI GAATG_Cn' Cuts at: 4175 8165 8258 4175 Size: 3990 93 6571 Fragments arranged by size: 6571 3990 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2253 2253 Size: 10654 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3356 4382 4821 4836 5665 5921 6360 Size: 399 1566 1026 439 15 829 256 439 Cuts at: 6360 6504 7136 7692 7753 7973 8715 8800 9871 Size: 144 632 556 61 220 742 85 1071 Cuts at: 9871 1391 Size: 2174 Fragments arranged by size: 2174 1566 1071 1026 829 742 632 556 439 439 399 256 220 144 85 61 15 BspEI T'CCGG_A Cuts at: 10317 10317 Size: 10654 BspHI T'CATG_A Cuts at: 3409 7707 8543 10273 3409 Size: 4298 836 1730 3790 Fragments arranged by size: 4298 3790 1730 836 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2812 8009 844 Size: 1968 5197 3489 Fragments arranged by size: 5197 3489 1968 BsrBI CCG'CTC Cuts at: 1394 1823 7275 8541 1394 Size: 429 5452 1266 3507 Fragments arranged by size: 5452 3507 1266 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5400 7838 9149 9323 65 Size: 1183 4152 2438 1311 174 1396 Fragments arranged by size: 4152 2438 1396 1311 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7157 9298 9485 1700 Size: 110 5347 2141 187 2869 Fragments arranged by size: 5347 2869 2141 187 110 BsrGI T'GTAC_A Cuts at: 96 2773 96 Size: 2677 7977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10654 BssSI C'ACGA_G Cuts at: 5790 6222 6558 7008 8714 9726 5790 Size: 432 336 450 1706 1012 6718 Fragments arranged by size: 6718 1706 1012 450 432 336 BstAPI GCAn_nnn'nTGC Cuts at: 794 2846 3061 6083 794 Size: 2052 215 3022 5365 Fragments arranged by size: 5365 3022 2052 215 BstBI TT'CG_AA Cuts at: 2007 9458 2007 Size: 7451 3203 BstEII G'GTnAC_C Cuts at: 1184 5273 5927 1184 Size: 4089 654 5911 Fragments arranged by size: 5911 4089 654 BstXI CCAn_nnnn'nTGG Cuts at: 5590 5603 6655 5590 Size: 13 1052 9589 Fragments arranged by size: 9589 1052 13 BstYI r'GATC_y Cuts at: 1972 3118 3544 4480 5090 5191 7701 7984 8351 Size: 1146 426 936 610 101 2510 283 367 Cuts at: 8351 8736 8753 10205 10291 10303 10649 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 2510 1977 1452 1146 936 610 426 385 367 346 283 101 86 17 12 BstZ17I GTA'TAC Cuts at: 7356 7356 Size: 10654 Bsu36I CC'TnA_GG Cuts at: 2928 3540 5923 2928 Size: 612 2383 7659 Fragments arranged by size: 7659 2383 612 BtgI C'CryG_G Cuts at: 513 1065 5505 7017 7242 513 Size: 552 4440 1512 225 3925 Fragments arranged by size: 4440 3925 1512 552 225 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 9915 Fragments arranged by size: 9915 683 56 BtsI GCAGTG_nn' Cuts at: 2398 6022 7312 8170 8964 8984 2398 Size: 3624 1290 858 794 20 4068 Fragments arranged by size: 4068 3624 1290 858 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10654 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 6755 6790 529 Size: 35 6191 35 4393 Fragments arranged by size: 6191 4393 35 35 DraI TTT'AAA Cuts at: 7958 7958 Size: 10654 DraIII CAC_nnn'GTG Cuts at: 4052 4052 Size: 10654 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9661 809 Size: 507 8345 1802 Fragments arranged by size: 8345 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 5734 7245 7266 Size: 54 1425 50 275 18 3904 1511 21 Cuts at: 7266 8101 8990 10619 8 Size: 835 889 1629 43 Fragments arranged by size: 3904 1629 1511 1425 889 835 275 54 50 43 21 18 EagI C'GGCC_G Cuts at: 1537 1812 8101 1537 Size: 275 6289 4090 Fragments arranged by size: 6289 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2430 2442 5031 5244 5700 5853 7053 7173 Size: 1077 12 2589 213 456 153 1200 120 Cuts at: 7173 7308 8584 1353 Size: 135 1276 3423 Fragments arranged by size: 3423 2589 1276 1200 1077 456 213 153 135 120 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9241 9615 9761 387 Size: 1064 138 7652 374 146 1280 Fragments arranged by size: 7652 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 7690 7690 Size: 10654 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2140 2171 2265 2472 2538 3099 3252 Size: 393 508 31 94 207 66 561 153 Cuts at: 3252 3498 3678 3999 4272 4282 4395 5877 5913 Size: 246 180 321 273 10 113 1482 36 Cuts at: 5913 6195 6328 6408 6465 6684 6946 7077 7535 Size: 282 133 80 57 219 262 131 458 Cuts at: 7535 7667 7706 7844 7854 8717 9314 10101 10369 Size: 132 39 138 10 863 597 787 268 Cuts at: 10369 1239 Size: 1524 Fragments arranged by size: 1524 1482 863 787 597 561 508 458 393 321 282 273 268 262 246 219 207 180 153 138 133 132 131 113 94 80 66 57 39 36 31 10 10 EcoNI CCTnn'n_nnAGG Cuts at: 3089 4767 6129 6680 3089 Size: 1678 1362 551 7063 Fragments arranged by size: 7063 1678 1362 551 EcoO109I rG'GnC_Cy Cuts at: 3081 5329 5524 7190 3081 Size: 2248 195 1666 6545 Fragments arranged by size: 6545 2248 1666 195 EcoRI G'AATT_C Cuts at: 3109 3532 7605 7963 8341 3109 Size: 423 4073 358 378 5422 Fragments arranged by size: 5422 4073 423 378 358 EcoRV GAT'ATC Cuts at: 2713 4777 5941 6886 2713 Size: 2064 1164 945 6481 Fragments arranged by size: 6481 2064 1164 945 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 7672 7672 Size: 10654 FspI TGC'GCA Cuts at: 9160 9160 Size: 10654 HaeII r_GCGC'y Cuts at: 1678 9801 1678 Size: 8123 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2207 2239 2513 2545 2921 Size: 32 135 32 635 32 274 32 376 Cuts at: 2921 2953 2978 3010 3995 4027 4108 4140 4376 Size: 32 25 32 985 32 81 32 236 Cuts at: 4376 4408 4472 4504 4889 4915 4921 4947 5666 Size: 32 64 32 385 26 6 26 719 Cuts at: 5666 5698 5930 5962 6323 6355 6461 6493 7052 Size: 32 232 32 361 32 106 32 559 Cuts at: 7052 7084 7679 7711 9295 9327 9369 9401 1373 Size: 32 595 32 1584 32 42 32 2626 Fragments arranged by size: 2626 1584 985 719 635 595 559 385 376 361 274 236 232 135 106 81 64 42 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 26 26 25 6 HincII GTy'rAC Cuts at: 669 1657 1938 2940 5297 7998 8244 669 Size: 988 281 1002 2357 2701 246 3079 Fragments arranged by size: 3079 2701 2357 1002 988 281 246 HindIII A'AGCT_T Cuts at: 748 4269 4717 748 Size: 3521 448 6685 Fragments arranged by size: 6685 3521 448 HpaI GTT'AAC Cuts at: 8244 8244 Size: 10654 KpnI G_GTAC'C Cuts at: 7979 7979 Size: 10654 MfeI C'AATT_G Cuts at: 5865 8253 5865 Size: 2388 8266 MluI A'CGCG_T Cuts at: 10530 10530 Size: 10654 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2040 2193 2433 2727 6547 9768 9952 1305 Size: 735 153 240 294 3820 3221 184 2007 Fragments arranged by size: 3820 3221 2007 735 294 240 184 153 MscI TGG'CCA Cuts at: 10 64 1489 1832 5736 7247 7268 10 Size: 54 1425 343 3904 1511 21 3396 Fragments arranged by size: 3904 3396 1511 1425 343 54 21 MslI CAynn'nnrTG Cuts at: 518 1166 2747 3221 5138 5736 6015 6665 7746 Size: 648 1581 474 1917 598 279 650 1081 Cuts at: 7746 7864 7935 8612 8971 9130 518 Size: 118 71 677 359 159 2042 Fragments arranged by size: 2042 1917 1581 1081 677 650 648 598 474 359 279 159 118 71 MspA1I CmG'CkG Cuts at: 1606 2243 6063 6281 6655 7580 7846 8070 8747 Size: 637 3820 218 374 925 266 224 677 Cuts at: 8747 9895 10140 1606 Size: 1148 245 2120 Fragments arranged by size: 3820 2120 1148 925 677 637 374 266 245 224 218 NcoI C'CATG_G Cuts at: 513 1065 5505 7017 7242 513 Size: 552 4440 1512 225 3925 Fragments arranged by size: 4440 3925 1512 552 225 NdeI CA'TA_TG Cuts at: 387 2599 387 Size: 2212 8442 NheI G'CTAG_C Cuts at: 1052 1052 Size: 10654 NotI GC'GGCC_GC Cuts at: 8101 8101 Size: 10654 NsiI A_TGCA'T Cuts at: 3480 3480 Size: 10654 NspI r_CATG'y Cuts at: 1132 1229 2340 2780 2824 8012 9557 1132 Size: 97 1111 440 44 5188 1545 2229 Fragments arranged by size: 5188 2229 1545 1111 440 97 44 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 10654 PciI A'CATG_T Cuts at: 2336 2776 9553 2336 Size: 440 6777 3437 Fragments arranged by size: 6777 3437 440 PflMI CCAn_nnn'nTGG Cuts at: 1108 4249 4687 4793 5356 5506 1108 Size: 3141 438 106 563 150 6256 Fragments arranged by size: 6256 3141 563 438 150 106 PfoI T'CCnGG_A Cuts at: 5766 6481 7683 5766 Size: 715 1202 8737 Fragments arranged by size: 8737 1202 715 PmeI GTTT'AAAC Cuts at: 7958 7958 Size: 10654 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3616 3648 8723 8755 10236 10268 3616 Size: 32 5075 32 1481 32 4002 Fragments arranged by size: 5075 4002 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3081 5329 5524 3081 Size: 2248 195 8211 Fragments arranged by size: 8211 2248 195 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 10654 PsiI TTA'TAA Cuts at: 8224 8224 Size: 10654 PstI C_TGCA'G Cuts at: 830 2907 3066 4501 8006 830 Size: 2077 159 1435 3505 3478 Fragments arranged by size: 3505 3478 2077 1435 159 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10654 PvuII CAG'CTG Cuts at: 2243 6281 7580 7846 2243 Size: 4038 1299 266 5051 Fragments arranged by size: 5051 4038 1299 266 SacI G_AGCT'C Cuts at: 7692 7692 Size: 10654 SalI G'TCGA_C Cuts at: 1936 7996 1936 Size: 6060 4594 SanDI GG'GwC_CC Cuts at: 5524 5524 Size: 10654 SapI GCTCTTCn'nnn_ Cuts at: 5853 7173 5853 Size: 1320 9334 SbfI CC_TGCA'GG Cuts at: 8006 8006 Size: 10654 ScaI AGT'ACT Cuts at: 4849 5437 7475 8902 10614 4849 Size: 588 2038 1427 1712 4889 Fragments arranged by size: 4889 2038 1712 1427 588 SfcI C'TryA_G Cuts at: 826 1045 2903 3062 4497 4512 6745 7110 7296 Size: 219 1858 159 1435 15 2233 365 186 Cuts at: 7296 8002 9137 9818 10009 826 Size: 706 1135 681 191 1471 Fragments arranged by size: 2233 1858 1471 1435 1135 706 681 365 219 191 186 159 15 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10654 SgrAI Cr'CCGG_yG Cuts at: 1700 7157 1700 Size: 5457 5197 SmaI CCC'GGG Cuts at: 1872 7981 1872 Size: 6109 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3628 5241 5382 5518 7592 8758 Size: 197 926 1685 1613 141 136 2074 1166 Cuts at: 8758 9659 9921 820 Size: 901 262 1553 Fragments arranged by size: 2074 1685 1613 1553 1166 926 901 262 197 141 136 SnaBI TAC'GTA Cuts at: 493 7951 8445 493 Size: 7458 494 2702 Fragments arranged by size: 7458 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 10654 SphI G_CATG'C Cuts at: 1132 8012 1132 Size: 6880 3774 SspI AAT'ATT Cuts at: 5 52 8578 5 Size: 47 8526 2081 Fragments arranged by size: 8526 2081 47 StuI AGG'CCT Cuts at: 1517 5462 6183 1517 Size: 3945 721 5988 Fragments arranged by size: 5988 3945 721 StyI C'CwwG_G Cuts at: 513 1065 2509 4132 4449 5505 5958 6184 6294 Size: 552 1444 1623 317 1056 453 226 110 Cuts at: 6294 6804 7017 7242 8093 513 Size: 510 213 225 851 3074 Fragments arranged by size: 3074 1623 1444 1056 851 552 510 453 317 226 225 213 110 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3009 5221 6383 7858 8681 8698 8851 Size: 1255 1429 2212 1162 1475 823 17 153 Cuts at: 8851 9036 9481 325 Size: 185 445 1498 Fragments arranged by size: 2212 1498 1475 1429 1255 1162 823 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2773 3766 4847 Size: 274 80 33 51 1443 796 993 1081 Cuts at: 4847 5435 5585 6741 7473 8900 10612 96 Size: 588 150 1156 732 1427 1712 138 Fragments arranged by size: 1712 1443 1427 1156 1081 993 796 732 588 274 150 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2377 2394 2409 2426 3005 3037 3348 3369 3380 Size: 17 15 17 579 32 311 21 11 Cuts at: 3380 3401 6988 7020 7113 7145 7489 7521 10614 Size: 21 3587 32 93 32 344 32 3093 Cuts at: 10614 10646 2377 Size: 32 2385 Fragments arranged by size: 3587 3093 2385 579 344 311 93 32 32 32 32 32 21 21 17 17 15 11 Tth111I GACn'n_nGTC Cuts at: 1316 5134 1316 Size: 3818 6836 XbaI T'CTAG_A Cuts at: 2131 7990 2131 Size: 5859 4795 XcmI CCAnnnn_n'nnnnTGG Cuts at: 6446 7261 6446 Size: 815 9839 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10654 XmaI C'CCGG_G Cuts at: 1870 7979 1870 Size: 6109 4545 XmnI GAAnn'nnTTC Cuts at: 7475 8783 7475 Size: 1308 9346 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8462 276 Size: 53 83 186 936 6928 2468 Fragments arranged by size: 6928 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BbeI BsiWI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI