Restriction map for pFN21ASDB0046
 (Linear) (Six-base) MAPSORT of: KIBB0046.seq  Check: 3425  from: 1  to: 5925



KIBB0046.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:18  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     789    5925 
   Size:        789    5136
 
AccI GT'mk_AC

Cuts at:      0    5332    5925 
   Size:       5332     593
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     449     515    1076    1229    1475    1976    2249 
   Size:        117     332      66     561     153     246     501     273

Cuts at:   2249    3854    3890    4305    4661    5054    5644    5821    5831 
   Size:       1605      36     415     356     393     590     177      10

Cuts at:   5831    5925 
   Size:         94

  Fragments arranged by size: 

                1605     590     561     501     415     393     356     332
                 273     246     177     153     117      94      66      36
                  10
 
AflIII A'CryG_T

Cuts at:      0     313     753    1193    5925 
   Size:        313     440     440    4732

  Fragments arranged by size: 

                4732     440     440     313
 
AgeI A'CCGG_T

Cuts at:      0    5134    5925 
   Size:       5134     791
 
AleI CACnn'nnGTG

Cuts at:      0    1198    3992    4642    5841    5925 
   Size:       1198    2794     650    1199      84

  Fragments arranged by size: 

                2794    1199    1198     650      84
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1576    5925 
   Size:       1576    4349
 
AlwNI CAG_nnn'CTG

Cuts at:      0     289     904    1999    2473    2488    2664    3511    5359 
   Size:        289     615    1095     474      15     176     847    1848

Cuts at:   5359    5622    5704    5925 
   Size:        263      82     221

  Fragments arranged by size: 

                1848    1095     847     615     474     289     263     221
                 176      82      15
 
ApoI r'AATT_y

Cuts at:      0    1086    1509    1687    4406    5299    5309    5582    5925 
   Size:       1086     423     178    2719     893      10     273     343

  Fragments arranged by size: 

                2719    1086     893     423     343     273     178      10
            
 
AvaI C'yCGr_G

Cuts at:      0    4076    4337    5925 
   Size:       4076     261    1588

  Fragments arranged by size: 

                4076    1588     261
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    4572    4605    5925 
   Size:       4572      33    1320

  Fragments arranged by size: 

                4572    1320      33
 
BamHI G'GATC_C

Cuts at:      0    3168    5925 
   Size:       3168    2757
 
BanI G'GyrC_C

Cuts at:      0    2795    5925 
   Size:       2795    3130
 
BanII G_rGCy'C

Cuts at:      0    2813    3898    4481    5113    5669    5925 
   Size:       2813    1085     583     632     556     256

  Fragments arranged by size: 

                2813    1085     632     583     556     256
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     932    1332    1982    2113    2615    3112    3398    5407 
   Size:        932     400     650     131     502     497     286    2009

Cuts at:   5407    5925 
   Size:        518

  Fragments arranged by size: 

                2009     932     650     518     502     497     400     286
                 131
 
BbvCI CC'TCA_GC

Cuts at:      0     350    3698    5925 
   Size:        350    3348    2227

  Fragments arranged by size: 

                3348    2227     350
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1647    1723    5925 
   Size:       1647      76    4202

  Fragments arranged by size: 

                4202    1647      76
 
BfrBI ATG'CAT

Cuts at:      0    1455    5925 
   Size:       1455    4470
 
BglI GCCn_nnn'nGGC

Cuts at:      0    4203    4274    5925 
   Size:       4203      71    1651

  Fragments arranged by size: 

                4203    1651      71
 
BglII A'GATC_T

Cuts at:      0    1095    2457    5925 
   Size:       1095    1362    3468

  Fragments arranged by size: 

                3468    1362    1095
 
BlpI GC'TnA_GC

Cuts at:      0    2638    5925 
   Size:       2638    3287
 
Bme1580I G_kGCm'C

Cuts at:      0    2798    4337    5730    5925 
   Size:       2798    1539    1393     195

  Fragments arranged by size: 

                2798    1539    1393     195
 
BmgBI CAC'GTC

Cuts at:      0    1196    2955    5925 
   Size:       1196    1759    2970

  Fragments arranged by size: 

                2970    1759    1196
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     734    2361    3899    5385    5925 
   Size:        734    1627    1538    1486     540

  Fragments arranged by size: 

                1627    1538    1486     734     540
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    2065    2373    5925 
   Size:       2065     308    3552

  Fragments arranged by size: 

                3552    2065     308
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     242    1655    2259    2372    4172    4385    4442 
   Size:        148      94    1413     604     113    1800     213      57

Cuts at:   4442    4923    5512    5683    5925 
   Size:        481     589     171     242

  Fragments arranged by size: 

                1800    1413     604     589     481     242     213     171
                 148     113      94      57
 
Bpu10I CC'TnA_GC

Cuts at:      0     350    3698    4002    5925 
   Size:        350    3348     304    1923

  Fragments arranged by size: 

                3348    1923     350     304
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1626    3203    3344    3516    5590    5925 
   Size:       1626    1577     141     172    2074     335

  Fragments arranged by size: 

                2074    1626    1577     335     172     141
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    2893    5925 
   Size:       2893    3032
 
BsaHI Gr'CG_yC

Cuts at:      0    4129    5925 
   Size:       4129    1796
 
BsaWI w'CCGG_w

Cuts at:      0    5134    5925 
   Size:       5134     791
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0    4651    4681    4900    4930    5090    5120    5656    5686 
   Size:       4651      30     219      30     160      30     536      30

Cuts at:   5686    5925 
   Size:        239

  Fragments arranged by size: 

                4651     536     239     219     160      30      30      30
                  30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     248    1232    1996    3244    3841    3888    3921    4094 
   Size:        248     984     764    1248     597      47      33     173

Cuts at:   4094    4156    4443    5308    5587    5925 
   Size:         62     287     865     279     338

  Fragments arranged by size: 

                1248     984     865     764     597     338     287     279
                 248     173      62      47      33
 
BseYI C'CCAG_C

Cuts at:      0    1370    2664    3454    4069    4549    4920    5342    5557 
   Size:       1370    1294     790     615     480     371     422     215

Cuts at:   5557    5787    5925 
   Size:        230     138

  Fragments arranged by size: 

                1370    1294     790     615     480     422     371     230
                 215     138
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     839    4697    5717    5925 
   Size:        839    3858    1020     208

  Fragments arranged by size: 

                3858    1020     839     208
 
BsiHKAI G_wGCw'C

Cuts at:      0    1333    2359    3642    5669    5925 
   Size:       1333    1026    1283    2027     256

  Fragments arranged by size: 

                2027    1333    1283    1026     256
 
BsmI GAATG_Cn'

Cuts at:      0    2152    5925 
   Size:       2152    3773
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     230    5925 
   Size:        230    5695
 
Bsp1286I G_dGCh'C

Cuts at:      0    1333    2359    2798    2813    3642    3898    4337    4481 
   Size:       1333    1026     439      15     829     256     439     144

Cuts at:   4481    5113    5669    5730    5925 
   Size:        632     556      61     195

  Fragments arranged by size: 

                1333    1026     829     632     556     439     439     256
                 195     144      61      15
 
BspHI T'CATG_A

Cuts at:      0    1386    5684    5925 
   Size:       1386    4298     241

  Fragments arranged by size: 

                4298    1386     241
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     789    5925 
   Size:        789    5136
 
BsrBI CCG'CTC

Cuts at:      0    5252    5925 
   Size:       5252     673
 
BsrDI GCAATG_nn'

Cuts at:      0    3377    5815    5925 
   Size:       3377    2438     110

  Fragments arranged by size: 

                3377    2438     110
 
BsrFI r'CCGG_y

Cuts at:      0    5134    5925 
   Size:       5134     791
 
BsrGI T'GTAC_A

Cuts at:      0     750    5925 
   Size:        750    5175
 
BssSI C'ACGA_G

Cuts at:      0    3767    4199    4535    4985    5925 
   Size:       3767     432     336     450     940

  Fragments arranged by size: 

                3767     940     450     432     336
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     823    1038    4060    5925 
   Size:        823     215    3022    1865

  Fragments arranged by size: 

                3022    1865     823     215
 
BstEII G'GTnAC_C

Cuts at:      0    3250    3904    5925 
   Size:       3250     654    2021

  Fragments arranged by size: 

                3250    2021     654
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    3567    3580    4632    5925 
   Size:       3567      13    1052    1293

  Fragments arranged by size: 

                3567    1293    1052      13
 
BstYI r'GATC_y

Cuts at:      0    1095    1521    2457    3067    3168    5678    5925 
   Size:       1095     426     936     610     101    2510     247

  Fragments arranged by size: 

                2510    1095     936     610     426     247     101
 
BstZ17I GTA'TAC

Cuts at:      0    5333    5925 
   Size:       5333     592
 
Bsu36I CC'TnA_GG

Cuts at:      0     905    1517    3900    5925 
   Size:        905     612    2383    2025

  Fragments arranged by size: 

                2383    2025     905     612
 
BtgI C'CryG_G

Cuts at:      0    3482    4994    5219    5925 
   Size:       3482    1512     225     706

  Fragments arranged by size: 

                3482    1512     706     225
 
BtsI GCAGTG_nn'

Cuts at:      0     375    3999    5289    5925 
   Size:        375    3624    1290     636

  Fragments arranged by size: 

                3624    1290     636     375
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    4732    4767    5925 
   Size:       4732      35    1158

  Fragments arranged by size: 

                4732    1158      35
 
DraIII CAC_nnn'GTG

Cuts at:      0    2029    5925 
   Size:       2029    3896
 
EaeI y'GGCC_r

Cuts at:      0    3711    5222    5243    5925 
   Size:       3711    1511      21     682

  Fragments arranged by size: 

                3711    1511     682      21
 
EarI CTCTTCn'nnn_

Cuts at:      0     407     419    3008    3221    3677    3830    5030    5150 
   Size:        407      12    2589     213     456     153    1200     120

Cuts at:   5150    5285    5925 
   Size:        135     640

  Fragments arranged by size: 

                2589    1200     640     456     407     213     153     135
                 120      12
 
EcoICRI GAG'CTC

Cuts at:      0    5667    5925 
   Size:       5667     258
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     117     148     242     449     515    1076    1229    1475 
   Size:        117      31      94     207      66     561     153     246

Cuts at:   1475    1655    1976    2249    2259    2372    3854    3890    4172 
   Size:        180     321     273      10     113    1482      36     282

Cuts at:   4172    4305    4385    4442    4661    4923    5054    5512    5644 
   Size:        133      80      57     219     262     131     458     132

Cuts at:   5644    5683    5821    5831    5925 
   Size:         39     138      10      94

  Fragments arranged by size: 

                1482     561     458     321     282     273     262     246
                 219     207     180     153     138     133     132     131
                 117     113      94      94      80      66      57      39
                  36      31      10      10
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1066    2744    4106    4657    5925 
   Size:       1066    1678    1362     551    1268

  Fragments arranged by size: 

                1678    1362    1268    1066     551
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1058    3306    3501    5167    5925 
   Size:       1058    2248     195    1666     758

  Fragments arranged by size: 

                2248    1666    1058     758     195
 
EcoRI G'AATT_C

Cuts at:      0    1086    1509    5582    5925 
   Size:       1086     423    4073     343

  Fragments arranged by size: 

                4073    1086     423     343
 
EcoRV GAT'ATC

Cuts at:      0     690    2754    3918    4863    5925 
   Size:        690    2064    1164     945    1062

  Fragments arranged by size: 

                2064    1164    1062     945     690
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    5649    5925 
   Size:       5649     276
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     184     216     490     522     898     930     955     987 
   Size:        184      32     274      32     376      32      25      32

Cuts at:    987    1972    2004    2085    2117    2353    2385    2449    2481 
   Size:        985      32      81      32     236      32      64      32

Cuts at:   2481    2866    2892    2898    2924    3643    3675    3907    3939 
   Size:        385      26       6      26     719      32     232      32

Cuts at:   3939    4300    4332    4438    4470    5029    5061    5656    5688 
   Size:        361      32     106      32     559      32     595      32

Cuts at:   5688    5925 
   Size:        237

  Fragments arranged by size: 

                 985     719     595     559     385     376     361     274
                 237     236     232     184     106      81      64      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      26      26      25
                   6
 
HincII GTy'rAC

Cuts at:      0     917    3274    5925 
   Size:        917    2357    2651

  Fragments arranged by size: 

                2651    2357     917
 
HindIII A'AGCT_T

Cuts at:      0    2246    2694    5925 
   Size:       2246     448    3231

  Fragments arranged by size: 

                3231    2246     448
 
MfeI C'AATT_G

Cuts at:      0    3842    5925 
   Size:       3842    2083
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      17     170     410     704    4524    5925 
   Size:         17     153     240     294    3820    1401

  Fragments arranged by size: 

                3820    1401     294     240     153      17
 
MscI TGG'CCA

Cuts at:      0    3713    5224    5245    5925 
   Size:       3713    1511      21     680

  Fragments arranged by size: 

                3713    1511     680      21
 
MslI CAynn'nnrTG

Cuts at:      0     724    1198    3115    3713    3992    4642    5723    5841 
   Size:        724     474    1917     598     279     650    1081     118

Cuts at:   5841    5912    5925 
   Size:         71      13

  Fragments arranged by size: 

                1917    1081     724     650     598     474     279     118
                  71      13
 
MspA1I CmG'CkG

Cuts at:      0     220    4040    4258    4632    5557    5823    5925 
   Size:        220    3820     218     374     925     266     102

  Fragments arranged by size: 

                3820     925     374     266     220     218     102
 
NcoI C'CATG_G

Cuts at:      0    3482    4994    5219    5925 
   Size:       3482    1512     225     706

  Fragments arranged by size: 

                3482    1512     706     225
 
NdeI CA'TA_TG

Cuts at:      0     576    5925 
   Size:        576    5349
 
NsiI A_TGCA'T

Cuts at:      0    1457    5925 
   Size:       1457    4468
 
NspI r_CATG'y

Cuts at:      0     317     757     801    5925 
   Size:        317     440      44    5124

  Fragments arranged by size: 

                5124     440     317      44
 
PciI A'CATG_T

Cuts at:      0     313     753    5925 
   Size:        313     440    5172

  Fragments arranged by size: 

                5172     440     313
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    2226    2664    2770    3333    3483    5925 
   Size:       2226     438     106     563     150    2442

  Fragments arranged by size: 

                2442    2226     563     438     150     106
 
PfoI T'CCnGG_A

Cuts at:      0    3743    4458    5660    5925 
   Size:       3743     715    1202     265

  Fragments arranged by size: 

                3743    1202     715     265
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1593    1625    5925 
   Size:       1593      32    4300

  Fragments arranged by size: 

                4300    1593      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    1058    3306    3501    5925 
   Size:       1058    2248     195    2424

  Fragments arranged by size: 

                2424    2248    1058     195
 
PstI C_TGCA'G

Cuts at:      0     884    1043    2478    5925 
   Size:        884     159    1435    3447

  Fragments arranged by size: 

                3447    1435     884     159
 
PvuII CAG'CTG

Cuts at:      0     220    4258    5557    5823    5925 
   Size:        220    4038    1299     266     102

  Fragments arranged by size: 

                4038    1299     266     220     102
 
SacI G_AGCT'C

Cuts at:      0    5669    5925 
   Size:       5669     256
 
SanDI GG'GwC_CC

Cuts at:      0    3501    5925 
   Size:       3501    2424
 
SapI GCTCTTCn'nnn_

Cuts at:      0    3830    5150    5925 
   Size:       3830    1320     775

  Fragments arranged by size: 

                3830    1320     775
 
ScaI AGT'ACT

Cuts at:      0    2826    3414    5452    5925 
   Size:       2826     588    2038     473

  Fragments arranged by size: 

                2826    2038     588     473
 
SfcI C'TryA_G

Cuts at:      0     880    1039    2474    2489    4722    5087    5273    5925 
   Size:        880     159    1435      15    2233     365     186     652

  Fragments arranged by size: 

                2233    1435     880     652     365     186     159      15
            
 
SgrAI Cr'CCGG_yG

Cuts at:      0    5134    5925 
   Size:       5134     791
 
SmlI C'TyrA_G

Cuts at:      0    1605    3218    3359    3495    5569    5925 
   Size:       1605    1613     141     136    2074     356

  Fragments arranged by size: 

                2074    1613    1605     356     141     136
 
StuI AGG'CCT

Cuts at:      0    3439    4160    5925 
   Size:       3439     721    1765

  Fragments arranged by size: 

                3439    1765     721
 
StyI C'CwwG_G

Cuts at:      0     486    2109    2426    3482    3935    4161    4271    4781 
   Size:        486    1623     317    1056     453     226     110     510

Cuts at:   4781    4994    5219    5925 
   Size:        213     225     706

  Fragments arranged by size: 

                1623    1056     706     510     486     453     317     226
                 225     213     110
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     986    3198    4360    5835    5925 
   Size:        986    2212    1162    1475      90

  Fragments arranged by size: 

                2212    1475    1162     986      90
 
TatI w'GTAC_w

Cuts at:      0     750    1743    2824    3412    3562    4718    5450    5925 
   Size:        750     993    1081     588     150    1156     732     475

  Fragments arranged by size: 

                1156    1081     993     750     732     588     475     150
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     354     371     386     403     982    1014    1325    1346 
   Size:        354      17      15      17     579      32     311      21

Cuts at:   1346    1357    1378    4965    4997    5090    5122    5466    5498 
   Size:         11      21    3587      32      93      32     344      32

Cuts at:   5498    5925 
   Size:        427

  Fragments arranged by size: 

                3587     579     427     354     344     311      93      32
                  32      32      32      21      21      17      17      15
                  11
 
Tth111I GACn'n_nGTC

Cuts at:      0    3111    5925 
   Size:       3111    2814
 
XbaI T'CTAG_A

Cuts at:      0     108    5925 
   Size:        108    5817
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    4423    5238    5925 
   Size:       4423     815     687

  Fragments arranged by size: 

                4423     815     687
 
XmnI GAAnn'nnTTC

Cuts at:      0    5452    5925 
   Size:       5452     473
 

 Enzymes that do cut:

     AarI     AccI     AcuI   AflIII     AgeI     AleI     AlfI    AlwNI
     ApoI     AvaI     BaeI    BamHI     BanI    BanII     BbsI    BbvCI
    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I     BtgI     BtsI    CspCI   DraIII
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI    Hin4I   HincII  HindIII     MfeI     MmeI     MscI     MslI
   MspA1I     NcoI     NdeI     NsiI     NspI     PciI    PflMI     PfoI
     PpiI    PpuMI     PstI    PvuII     SacI    SanDI     SapI     ScaI
     SfcI    SgrAI     SmlI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII     AhdI     AloI     ApaI
    ApaLI     AscI     AseI    AsiSI    AvrII     BbeI     BcgI     BclI
     BmtI    BsaAI    BsaBI    BsiEI    BsiWI    BspEI   BssHII    BstBI
    BtgZI     ClaI     DraI     DrdI     EagI     EciI     FseI     FspI
    FspAI    HaeII     HpaI     KasI     KpnI     MluI     NaeI     NarI
   NgoMIV     NheI     NotI     NruI     PacI     PasI     PmeI     PmlI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII
     SalI     SbfI    SexAI     SfiI     SfoI     SgfI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     SwaI     XhoI     XmaI     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com