(Circular) (Six-base) MAPSORT of: pFN21ASDB0037.seq Check: 748 from: 1 to: 6142 pFN21ASDB0037 6142 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2676 3952 278 Size: 53 83 186 936 1140 1276 2468 Fragments arranged by size: 2468 1276 1140 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3312 3485 1937 Size: 1375 173 4594 Fragments arranged by size: 4594 1375 173 Acc65I G'GTAC_C Cuts at: 3169 3463 3169 Size: 294 5848 AclI AA'CG_TT Cuts at: 1563 4269 4642 1563 Size: 2706 373 3063 Fragments arranged by size: 3063 2706 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2627 2798 4205 5589 5857 1632 Size: 995 171 1407 1384 268 1917 Fragments arranged by size: 1917 1407 1384 995 268 171 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5945 AflIII A'CryG_T Cuts at: 1203 5041 6018 1203 Size: 3838 977 1327 Fragments arranged by size: 3838 1327 977 AgeI A'CCGG_T Cuts at: 4973 4973 Size: 6142 AhdI GACnn_n'nnGTC Cuts at: 4871 4871 Size: 6142 AleI CACnn'nnGTG Cuts at: 1166 2991 1166 Size: 1825 4317 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6142 AlwNI CAG_nnn'CTG Cuts at: 3885 5457 3885 Size: 1572 4570 ApaLI G'TGCA_C Cuts at: 4199 5355 4199 Size: 1156 4986 ApoI r'AATT_y Cuts at: 1493 2552 2572 3399 3451 3677 3829 5938 1493 Size: 1059 20 827 52 226 152 2109 1697 Fragments arranged by size: 2109 1697 1059 827 226 152 52 20 AseI AT'TA_AT Cuts at: 160 1032 4696 160 Size: 872 3664 1606 Fragments arranged by size: 3664 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 AvaI C'yCGr_G Cuts at: 1870 1943 2757 3467 1870 Size: 73 814 710 4545 Fragments arranged by size: 4545 814 710 73 BamHI G'GATC_C Cuts at: 3261 3472 3839 3261 Size: 211 367 5564 Fragments arranged by size: 5564 367 211 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2138 3169 3463 4918 618 Size: 325 217 627 351 1031 294 1455 1842 Fragments arranged by size: 1842 1455 1031 627 351 325 294 217 BanII G_rGCy'C Cuts at: 1391 3245 3461 1391 Size: 1854 216 4072 Fragments arranged by size: 4072 1854 216 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2668 3153 928 Size: 971 769 485 3917 Fragments arranged by size: 3917 971 769 485 BbvCI CC'TCA_GC Cuts at: 3025 3025 Size: 6142 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4333 4367 1903 Size: 34 2396 34 3678 Fragments arranged by size: 3678 2396 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4034 5244 4034 Size: 1210 4932 BclI T'GATC_A Cuts at: 1548 1548 Size: 6142 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4753 136 Size: 107 122 71 631 605 3081 1525 Fragments arranged by size: 3081 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2832 6137 2832 Size: 3305 2837 BlpI GC'TnA_GC Cuts at: 3559 3559 Size: 6142 Bme1580I G_kGCm'C Cuts at: 1790 2859 4203 5359 1790 Size: 1069 1344 1156 2573 Fragments arranged by size: 2573 1344 1156 1069 BmgBI CAC'GTC Cuts at: 1318 2683 3062 1318 Size: 1365 379 4398 Fragments arranged by size: 4398 1365 379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3873 4831 442 Size: 416 53 83 399 24 2456 958 1753 Fragments arranged by size: 2456 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6142 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4802 1239 Size: 3563 2579 Bpu10I CC'TnA_GC Cuts at: 3025 3025 Size: 6142 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4267 5132 5430 4267 Size: 865 298 4979 Fragments arranged by size: 4979 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2429 2663 4805 882 Size: 617 930 234 2142 2219 Fragments arranged by size: 2219 2142 930 617 234 BsaAI yAC'GTr Cuts at: 493 1204 3439 3933 493 Size: 711 2235 494 2702 Fragments arranged by size: 2702 2235 711 494 BsaBI GATnn'nnATC Cuts at: 2039 2292 3036 6136 2039 Size: 253 744 3100 2045 Fragments arranged by size: 3100 2045 744 253 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2673 3949 4331 4966 Size: 53 83 186 936 1140 1276 382 635 Cuts at: 4966 275 Size: 1451 Fragments arranged by size: 1451 1276 1140 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4575 4973 5247 5394 5805 4575 Size: 398 274 147 411 4912 Fragments arranged by size: 4912 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5917 Fragments arranged by size: 5917 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2978 1186 Size: 1792 4350 BseYI C'CCAG_C Cuts at: 2225 5345 5841 2225 Size: 3120 496 2526 Fragments arranged by size: 3120 2526 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5569 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3592 4353 5381 1540 Size: 275 186 1591 761 1028 2301 Fragments arranged by size: 2301 1591 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4203 4288 5359 4203 Size: 85 1071 4986 Fragments arranged by size: 4986 1071 85 BsmI GAATG_Cn' Cuts at: 2520 3653 3746 2520 Size: 1133 93 4916 Fragments arranged by size: 4916 1133 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2859 3245 3461 4203 4288 5359 1391 Size: 399 1069 386 216 742 85 1071 2174 Fragments arranged by size: 2174 1071 1069 742 399 386 216 85 BspEI T'CCGG_A Cuts at: 5805 5805 Size: 6142 BspHI T'CATG_A Cuts at: 4031 5761 4031 Size: 1730 4412 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3497 844 Size: 2653 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4029 1394 Size: 429 2206 3507 Fragments arranged by size: 3507 2206 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2102 2769 4637 4811 65 Size: 1183 854 667 1868 174 1396 Fragments arranged by size: 1868 1396 1183 854 667 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2075 4786 4973 1700 Size: 110 265 2711 187 2869 Fragments arranged by size: 2869 2711 265 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6142 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6142 BssSI C'ACGA_G Cuts at: 4202 5214 4202 Size: 1012 5130 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6142 BstBI TT'CG_AA Cuts at: 2007 4946 2007 Size: 2939 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6142 BstYI r'GATC_y Cuts at: 1972 2832 3261 3295 3472 3839 4224 4241 5693 Size: 860 429 34 177 367 385 17 1452 Cuts at: 5693 5779 5791 6137 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 860 429 385 367 346 177 86 34 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4633 Fragments arranged by size: 4633 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3023 487 Size: 683 56 1797 3606 Fragments arranged by size: 3606 1797 683 56 BtsI GCAGTG_nn' Cuts at: 3658 4452 4472 3658 Size: 794 20 5328 Fragments arranged by size: 5328 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6142 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6107 DraI TTT'AAA Cuts at: 2394 3446 2394 Size: 1052 5090 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5149 809 Size: 507 3833 1802 Fragments arranged by size: 3833 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2073 3589 Size: 54 1425 50 275 18 195 48 1516 Cuts at: 3589 4478 6107 8 Size: 889 1629 43 Fragments arranged by size: 1629 1516 1425 889 275 195 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3589 1537 Size: 275 1777 4090 Fragments arranged by size: 4090 1777 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2346 2928 4072 1353 Size: 993 582 1144 3423 Fragments arranged by size: 3423 1144 993 582 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2718 4729 5103 5249 387 Size: 1064 138 1129 2011 374 146 1280 Fragments arranged by size: 2011 1280 1129 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2627 2798 4205 4802 5589 5857 1239 Size: 393 995 171 1407 597 787 268 1524 Fragments arranged by size: 1524 1407 995 787 597 393 268 171 EcoNI CCTnn'n_nnAGG Cuts at: 2175 2175 Size: 6142 EcoRI G'AATT_C Cuts at: 3451 3829 3451 Size: 378 5764 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3035 3035 Size: 6142 FspI TGC'GCA Cuts at: 4648 4648 Size: 6142 HaeII r_GCGC'y Cuts at: 1678 5289 1678 Size: 3611 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2911 2943 3330 3353 3362 Size: 32 135 32 1339 32 387 23 9 Cuts at: 3362 3385 4783 4815 4857 4889 1373 Size: 23 1398 32 42 32 2626 Fragments arranged by size: 2626 1398 1339 387 135 42 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 3486 3732 669 Size: 988 281 1548 246 3079 Fragments arranged by size: 3079 1548 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6142 HpaI GTT'AAC Cuts at: 3732 3732 Size: 6142 KpnI G_GTAC'C Cuts at: 3173 3467 3173 Size: 294 5848 MfeI C'AATT_G Cuts at: 2580 3741 2580 Size: 1161 4981 MluI A'CGCG_T Cuts at: 6018 6018 Size: 6142 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2337 5256 5440 1305 Size: 1032 2919 184 2007 Fragments arranged by size: 2919 2007 1032 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 10 Size: 54 1425 343 195 4125 Fragments arranged by size: 4125 1425 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2444 2991 4100 4459 4618 518 Size: 648 1278 547 1109 359 159 2042 Fragments arranged by size: 2042 1278 1109 648 547 359 159 MspA1I CmG'CkG Cuts at: 1606 3558 4235 5383 5628 1606 Size: 1952 677 1148 245 2120 Fragments arranged by size: 2120 1952 1148 677 245 NaeI GCC'GGC Cuts at: 2077 2077 Size: 6142 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4633 Fragments arranged by size: 4633 957 552 NdeI CA'TA_TG Cuts at: 387 2845 387 Size: 2458 3684 NgoMIV G'CCGG_C Cuts at: 2075 2075 Size: 6142 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6142 NotI GC'GGCC_GC Cuts at: 3589 3589 Size: 6142 NspI r_CATG'y Cuts at: 1132 1229 3500 5045 1132 Size: 97 2271 1545 2229 Fragments arranged by size: 2271 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6142 PciI A'CATG_T Cuts at: 5041 5041 Size: 6142 PflMI CCAn_nnn'nTGG Cuts at: 1108 2686 1108 Size: 1578 4564 PmeI GTTT'AAAC Cuts at: 3446 3446 Size: 6142 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4211 4243 5724 5756 4211 Size: 32 1481 32 4597 Fragments arranged by size: 4597 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6142 PsiI TTA'TAA Cuts at: 3712 3712 Size: 6142 PstI C_TGCA'G Cuts at: 830 3494 830 Size: 2664 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6142 SalI G'TCGA_C Cuts at: 1936 3484 1936 Size: 1548 4594 SbfI CC_TGCA'GG Cuts at: 3494 3494 Size: 6142 ScaI AGT'ACT Cuts at: 2819 4390 6102 2819 Size: 1571 1712 2859 Fragments arranged by size: 2859 1712 1571 SfcI C'TryA_G Cuts at: 826 1045 3309 3490 4625 5306 5497 826 Size: 219 2264 181 1135 681 191 1471 Fragments arranged by size: 2264 1471 1135 681 219 191 181 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6142 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6142 SmaI CCC'GGG Cuts at: 1872 3469 1872 Size: 1597 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4246 5147 5409 820 Size: 197 926 2303 901 262 1553 Fragments arranged by size: 2303 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3439 3933 493 Size: 2946 494 2702 Fragments arranged by size: 2946 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6142 SphI G_CATG'C Cuts at: 1132 3500 1132 Size: 2368 3774 SspI AAT'ATT Cuts at: 5 52 4066 5 Size: 47 4014 2081 Fragments arranged by size: 4014 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6142 StyI C'CwwG_G Cuts at: 513 1065 2022 3581 513 Size: 552 957 1559 3074 Fragments arranged by size: 3074 1559 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4169 4186 4339 4524 4969 325 Size: 1255 2589 17 153 185 445 1498 Fragments arranged by size: 2589 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2817 4388 6100 Size: 274 80 33 51 1443 840 1571 1712 Cuts at: 6100 96 Size: 138 Fragments arranged by size: 1712 1571 1443 840 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3238 3270 6102 6134 3238 Size: 32 2832 32 3246 Fragments arranged by size: 3246 2832 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6142 XbaI T'CTAG_A Cuts at: 2713 3478 2713 Size: 765 5377 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6142 XmaI C'CCGG_G Cuts at: 1870 3467 1870 Size: 1597 4545 XmnI GAAnn'nnTTC Cuts at: 4271 4271 Size: 6142 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2674 3950 276 Size: 53 83 186 936 1140 1276 2468 Fragments arranged by size: 2468 1276 1140 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI