Restriction map for
pFN21ASDB0028
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflII AgeI AhdI Alw44I
AlwNI Aor51HI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BclI BfrI BlnI
BsaAI BsaBI BsaHI BseAI BseRI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspMI BsrBI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstUI BstXI BstZ17I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HgaI Hin1I HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI PstI PvuI
RsrII SacII SalI SbfI ScaI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AclI 1239 AACGTT AA^CGTT
AcsI 1401 GAATTT [GA]^AATT[CT]
AflIII 752 ACATGT A^C[GA][CT]GT
AocI 890 CCTCAGG CC^T[ACGT]AGG
ApaI 305 GGGCCC GGGCC^C
ApoI 1401 GAATTT [GA]^AATT[CT]
AspHI 1467 GAGCTC G[AT]GC[AT]^C
AvaI 164 CTCGGG C^[CT]CG[GA]G
BanI 970 GGTGCC G^G[CT][GA]CC
BbsI 297 GAAGAC GAAGAC(2/6)
BciVI 1362 GGATAC GTATCC(6/5)
BfaI 1460 CTAG C^TAG
BglI 430 GCCCAGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 465 AGATCT A^GATCT
BlpI 1006 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1006 GCTCAGC GC^T[ACGT]AGC
BpuAI 297 GAAGAC GAAGAC(2/6)
BsaI 1033 GGTCTC GGTCTC(1/5)
BsaWI 119 TCCGGT [AT]^CCGG[AT]
BsgI 845 GTGCAG GTGCAG(16/14)
BsiHKAI 1467 GAGCTC G[AT]GC[AT]^C
BsoBI 164 CTCGGG C^[CT]CG[GA]G
BspHI 330 TCATGA T^CATGA
BspLU11I 752 ACATGT A^CATGT
BsrFI 1657 GCCGGT [GA]^CCGG[CT]
BstAPI 1489 GCATCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 890 CCTCAGG CC^T[ACGT]AGG
CelII 1006 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1657 GCCGGT [GA]^CCGG[CT]
DraI 103 TTTAAA TTT^AAA
Eco81I 890 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1140 CCAAGG C^C[AT][AT]GG
FauI 1283 CCCGC CCCGC(4/6)
MaeI 1460 CTAG C^TAG
MspA1I 1127 CAGCTG C[AC]G^C[GT]G
NspI 752 ACATGT [GA]CATG^[CT]
PflMI 1013 CCATCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1143 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 1239 AACGTT AA^CGTT
PvuII 1127 CAGCTG CAG^CTG
RcaI 330 TCATGA T^CATGA
SacI 1467 GAGCTC GAGCT^C
SapI 1558 GAAGAGC GCTCTTC(1/4)
SexAI 1189 ACCAGGT A^CC[AT]GGT
SspI 561 AATATT AAT^ATT
StyI 1140 CCAAGG C^C[AT][AT]GG
TaqI 1432 TCGA T^CGA
TthHB8I 1432 TCGA T^CGA
Van91I 1013 CCATCGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1217 CCAGGCAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 117 GGTCC G^G[AT]CC
1144 GGTCC
BalI 416 TGGCCA TGG^CCA
1060 TGGCCA
BsrDI 345 CATTGC GCAATG(2/0)
904 GCAATG
BssSI 619 CACGAG CACGAG(-5/-1)
1176 CACGAG
BstYI 465 AGATCT [GA]^GATC[CT]
582 AGATCC
HincII 3 GTCAAC GT[CT]^[GA]AC
710 GTCAAC
HindII 3 GTCAAC GT[CT]^[GA]AC
710 GTCAAC
MaeII 899 ACGT A^CGT
1240 ACGT
MflI 465 AGATCT [GA]^GATC[CT]
582 AGATCC
MluNI 416 TGGCCA TGG^CCA
1060 TGGCCA
MscI 416 TGGCCA TGG^CCA
1060 TGGCCA
MslI 316 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1495 CATGCCAGTG
PleI 52 GACTC GAGTC(4/5)
1183 GACTC
SmlI 202 CTCAAG C^T[CT][GA]AG
459 CTTGAG
StuI 1322 AGGCCT AGG^CCT
1586 AGGCCT
XhoII 465 AGATCT [GA]^GATC[CT]
582 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI