(Circular) (Six-base) MAPSORT of: pFN21ASDB0028.seq Check: 3046 from: 1 to: 6394 pFN21ASDB0028 6394 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4204 278 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3737 1937 Size: 1800 4594 Acc65I G'GTAC_C Cuts at: 3715 3715 Size: 6394 AclI AA'CG_TT Cuts at: 1563 3263 4521 4894 1563 Size: 1700 1258 373 3063 Fragments arranged by size: 3063 1700 1258 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2318 4457 5841 6109 1632 Size: 686 2139 1384 268 1917 Fragments arranged by size: 2139 1917 1384 686 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6197 AflIII A'CryG_T Cuts at: 1203 2775 5293 6270 1203 Size: 1572 2518 977 1327 Fragments arranged by size: 2518 1572 1327 977 AgeI A'CCGG_T Cuts at: 5225 5225 Size: 6394 AhdI GACnn_n'nnGTC Cuts at: 5123 5123 Size: 6394 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6394 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2361 1184 Size: 1177 5217 AlwNI CAG_nnn'CTG Cuts at: 4137 5709 4137 Size: 1572 4822 ApaI G_GGCC'C Cuts at: 2332 2332 Size: 6394 ApaLI G'TGCA_C Cuts at: 4451 5607 4451 Size: 1156 5238 ApoI r'AATT_y Cuts at: 1493 3424 3703 3929 4081 6190 1493 Size: 1931 279 226 152 2109 1697 Fragments arranged by size: 2109 1931 1697 279 226 152 AseI AT'TA_AT Cuts at: 160 1032 4948 160 Size: 872 3916 1606 Fragments arranged by size: 3916 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 AvaI C'yCGr_G Cuts at: 1870 1943 2187 3719 1870 Size: 73 244 1532 4545 Fragments arranged by size: 4545 1532 244 73 BamHI G'GATC_C Cuts at: 3724 4091 3724 Size: 367 6027 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2993 3715 5170 618 Size: 325 217 627 1206 722 1455 1842 Fragments arranged by size: 1842 1455 1206 722 627 325 217 BanII G_rGCy'C Cuts at: 1391 2332 3031 3494 3642 3654 3713 1391 Size: 941 699 463 148 12 59 4072 Fragments arranged by size: 4072 941 699 463 148 59 12 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2327 928 Size: 971 428 4995 Fragments arranged by size: 4995 971 428 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4585 4619 1903 Size: 34 2648 34 3678 Fragments arranged by size: 3678 2648 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3379 4286 5496 3379 Size: 907 1210 4277 Fragments arranged by size: 4277 1210 907 BclI T'GATC_A Cuts at: 1548 1548 Size: 6394 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2459 5005 136 Size: 107 122 71 631 605 787 2546 1525 Fragments arranged by size: 2546 1525 787 631 605 122 107 71 BglII A'GATC_T Cuts at: 2488 6389 2488 Size: 3901 2493 BlpI GC'TnA_GC Cuts at: 3030 3811 3030 Size: 781 5613 Bme1580I G_kGCm'C Cuts at: 1790 2332 2998 3303 4455 5611 1790 Size: 542 666 305 1152 1156 2573 Fragments arranged by size: 2573 1156 1152 666 542 305 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6394 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3412 3636 3648 Size: 416 53 83 399 24 1995 224 12 Cuts at: 3648 4125 5083 442 Size: 477 958 1753 Fragments arranged by size: 1995 1753 958 477 416 399 224 83 53 24 12 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6394 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3367 3367 Size: 6394 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2062 2805 3337 5054 1239 Size: 823 743 532 1717 2579 Fragments arranged by size: 2579 1717 823 743 532 Bpu10I CC'TnA_GC Cuts at: 3012 3012 Size: 6394 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2210 2503 4519 5384 5682 2210 Size: 293 2016 865 298 2922 Fragments arranged by size: 2922 2016 865 298 293 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3062 5057 882 Size: 617 1563 1995 2219 Fragments arranged by size: 2219 1995 1563 617 BsaAI yAC'GTr Cuts at: 493 1204 3691 4185 493 Size: 711 2487 494 2702 Fragments arranged by size: 2702 2487 711 494 BsaBI GATnn'nnATC Cuts at: 6388 6388 Size: 6394 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4201 4583 5218 275 Size: 53 83 186 936 2668 382 635 1451 Fragments arranged by size: 2668 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2142 4827 5225 5499 5646 6057 2142 Size: 2685 398 274 147 411 2479 Fragments arranged by size: 2685 2479 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3308 3338 1178 Size: 30 165 30 1905 30 4234 Fragments arranged by size: 4234 1905 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6394 BseYI C'CCAG_C Cuts at: 2997 3285 3475 3569 5597 6093 2997 Size: 288 190 94 2028 496 3298 Fragments arranged by size: 3298 2028 496 288 190 94 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2889 1163 Size: 573 1153 4668 Fragments arranged by size: 4668 1153 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3844 4605 5633 1540 Size: 275 186 1843 761 1028 2301 Fragments arranged by size: 2301 1843 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3494 4455 4540 5611 3494 Size: 961 85 1071 4277 Fragments arranged by size: 4277 1071 961 85 BsmI GAATG_Cn' Cuts at: 3905 3998 3905 Size: 93 6301 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2332 2998 3031 3303 3494 3642 3654 Size: 399 542 666 33 272 191 148 12 Cuts at: 3654 3713 4455 4540 5611 1391 Size: 59 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 666 542 399 272 191 148 85 59 33 12 BspEI T'CCGG_A Cuts at: 6057 6057 Size: 6394 BspHI T'CATG_A Cuts at: 2353 4283 6013 2353 Size: 1930 1730 2734 Fragments arranged by size: 2734 1930 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3749 844 Size: 2905 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4281 1394 Size: 429 2458 3507 Fragments arranged by size: 3507 2458 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2367 2934 4889 5063 65 Size: 1183 1119 567 1955 174 1396 Fragments arranged by size: 1955 1396 1183 1119 567 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3680 5038 5225 1700 Size: 110 1870 1358 187 2869 Fragments arranged by size: 2869 1870 1358 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6394 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6394 BssSI C'ACGA_G Cuts at: 2642 3199 4454 5466 2642 Size: 557 1255 1012 3570 Fragments arranged by size: 3570 1255 1012 557 BstAPI GCAn_nnn'nTGC Cuts at: 794 3518 794 Size: 2724 3670 BstBI TT'CG_AA Cuts at: 2007 5198 2007 Size: 3191 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6394 BstYI r'GATC_y Cuts at: 1972 2488 2605 3724 4091 4476 4493 5945 6031 Size: 516 117 1119 367 385 17 1452 86 Cuts at: 6031 6043 6389 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1119 516 385 367 346 117 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2914 2914 Size: 6394 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5842 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3022 487 Size: 683 56 1796 3859 Fragments arranged by size: 3859 1796 683 56 BtsI GCAGTG_nn' Cuts at: 2107 2564 2898 3910 4704 4724 2107 Size: 457 334 1012 794 20 3777 Fragments arranged by size: 3777 1012 794 457 334 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6394 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6359 DraI TTT'AAA Cuts at: 2128 3698 2128 Size: 1570 4824 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5401 809 Size: 507 4085 1802 Fragments arranged by size: 4085 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2439 3083 3093 Size: 54 1425 50 275 18 609 644 10 Cuts at: 3093 3841 4730 6359 8 Size: 748 889 1629 43 Fragments arranged by size: 1629 1425 889 748 644 609 275 54 50 43 18 10 EagI C'GGCC_G Cuts at: 1537 1812 3841 1537 Size: 275 2029 4090 Fragments arranged by size: 4090 2029 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2586 3546 3576 4324 1353 Size: 1233 960 30 748 3423 Fragments arranged by size: 3423 1233 960 748 30 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3096 3282 4981 5355 5501 387 Size: 1064 138 1507 186 1699 374 146 1280 Fragments arranged by size: 1699 1507 1280 1064 374 186 146 138 EcoICRI GAG'CTC Cuts at: 3492 3492 Size: 6394 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2318 2805 3337 4457 5054 5841 Size: 393 430 256 487 532 1120 597 787 Cuts at: 5841 6109 1239 Size: 268 1524 Fragments arranged by size: 1524 1120 787 597 532 487 430 393 268 256 EcoO109I rG'GnC_Cy Cuts at: 2328 2329 3167 2328 Size: 1 838 5555 Fragments arranged by size: 5555 838 1 EcoRI G'AATT_C Cuts at: 3703 4081 3703 Size: 378 6016 FspI TGC'GCA Cuts at: 4900 4900 Size: 6394 HaeII r_GCGC'y Cuts at: 1678 2067 2247 2937 5541 1678 Size: 389 180 690 2604 2531 Fragments arranged by size: 2604 2531 690 389 180 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2783 2815 3308 3340 5035 Size: 32 135 32 1211 32 493 32 1695 Cuts at: 5035 5067 5109 5141 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1695 1211 493 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2028 2735 3738 3984 669 Size: 988 281 90 707 1003 246 3079 Fragments arranged by size: 3079 1003 988 707 281 246 90 HindIII A'AGCT_T Cuts at: 748 748 Size: 6394 HpaI GTT'AAC Cuts at: 3984 3984 Size: 6394 KpnI G_GTAC'C Cuts at: 3719 3719 Size: 6394 MfeI C'AATT_G Cuts at: 3993 3993 Size: 6394 MluI A'CGCG_T Cuts at: 6270 6270 Size: 6394 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2998 3494 3642 5508 5692 1305 Size: 1693 496 148 1866 184 2007 Fragments arranged by size: 2007 1866 1693 496 184 148 MscI TGG'CCA Cuts at: 10 64 1489 1832 2441 3085 10 Size: 54 1425 343 609 644 3319 Fragments arranged by size: 3319 1425 644 609 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2343 3522 4352 4711 4870 518 Size: 648 1177 1179 830 359 159 2042 Fragments arranged by size: 2042 1179 1177 830 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3152 3810 4487 5635 5880 1606 Size: 1546 658 677 1148 245 2120 Fragments arranged by size: 2120 1546 1148 677 658 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5842 NdeI CA'TA_TG Cuts at: 387 387 Size: 6394 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6394 NotI GC'GGCC_GC Cuts at: 3841 3841 Size: 6394 NspI r_CATG'y Cuts at: 1132 1229 2779 3752 5297 1132 Size: 97 1550 973 1545 2229 Fragments arranged by size: 2229 1550 1545 973 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6394 PciI A'CATG_T Cuts at: 2775 5293 2775 Size: 2518 3876 PflMI CCAn_nnn'nTGG Cuts at: 1108 3042 1108 Size: 1934 4460 PmeI GTTT'AAAC Cuts at: 3698 3698 Size: 6394 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4463 4495 5976 6008 4463 Size: 32 1481 32 4849 Fragments arranged by size: 4849 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3167 3167 Size: 6394 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6394 PsiI TTA'TAA Cuts at: 3964 3964 Size: 6394 PspOMI G'GGCC_C Cuts at: 2328 2328 Size: 6394 PstI C_TGCA'G Cuts at: 830 3746 830 Size: 2916 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6394 PvuII CAG'CTG Cuts at: 3152 3152 Size: 6394 SacI G_AGCT'C Cuts at: 3494 3494 Size: 6394 SalI G'TCGA_C Cuts at: 1936 3736 1936 Size: 1800 4594 SapI GCTCTTCn'nnn_ Cuts at: 3576 3576 Size: 6394 SbfI CC_TGCA'GG Cuts at: 3746 3746 Size: 6394 ScaI AGT'ACT Cuts at: 4642 6354 4642 Size: 1712 4682 SexAI A'CCwGG_T Cuts at: 3212 3212 Size: 6394 SfcI C'TryA_G Cuts at: 826 1045 3742 4877 5558 5749 826 Size: 219 2697 1135 681 191 1471 Fragments arranged by size: 2697 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6394 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6394 SmaI CCC'GGG Cuts at: 1872 3721 1872 Size: 1849 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2225 2482 4498 5399 5661 820 Size: 197 926 282 257 2016 901 262 1553 Fragments arranged by size: 2016 1553 926 901 282 262 257 197 SnaBI TAC'GTA Cuts at: 493 3691 4185 493 Size: 3198 494 2702 Fragments arranged by size: 3198 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6394 SphI G_CATG'C Cuts at: 1132 3752 1132 Size: 2620 3774 SspI AAT'ATT Cuts at: 5 52 2586 4318 5 Size: 47 2534 1732 2081 Fragments arranged by size: 2534 2081 1732 47 StuI AGG'CCT Cuts at: 1517 3347 3611 1517 Size: 1830 264 4300 Fragments arranged by size: 4300 1830 264 StyI C'CwwG_G Cuts at: 513 1065 3163 3833 513 Size: 552 2098 670 3074 Fragments arranged by size: 3074 2098 670 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2981 3245 4421 4438 4591 4776 5221 Size: 1255 1401 264 1176 17 153 185 445 Cuts at: 5221 325 Size: 1498 Fragments arranged by size: 1498 1401 1255 1176 445 264 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2619 4640 6352 Size: 274 80 33 51 1443 642 2021 1712 Cuts at: 6352 96 Size: 138 Fragments arranged by size: 2021 1712 1443 642 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2543 2575 6354 6386 2543 Size: 32 3779 32 2551 Fragments arranged by size: 3779 2551 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6394 XbaI T'CTAG_A Cuts at: 3730 3730 Size: 6394 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3247 3247 Size: 6394 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6394 XmaI C'CCGG_G Cuts at: 1870 3719 1870 Size: 1849 4545 XmnI GAAnn'nnTTC Cuts at: 4523 4523 Size: 6394 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4202 276 Size: 53 83 186 936 2668 2468 Fragments arranged by size: 2668 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI