Restriction map for
pFN21ASDB0028
(Linear) (Six-base) MAPSORT of: KIBB0028.seq Check: 7962 from: 1 to: 1665
KIBB0028.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AclI AA'CG_TT
Cuts at: 0 1240 1665
Size: 1240 425
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1665
Size: 295 1370
AflIII A'CryG_T
Cuts at: 0 752 1665
Size: 752 913
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 338 1665
Size: 338 1327
ApaI G_GGCC'C
Cuts at: 0 309 1665
Size: 309 1356
ApoI r'AATT_y
Cuts at: 0 1401 1665
Size: 1401 264
AvaI C'yCGr_G
Cuts at: 0 164 1665
Size: 164 1501
BanI G'GyrC_C
Cuts at: 0 970 1665
Size: 970 695
BanII G_rGCy'C
Cuts at: 0 309 1008 1471 1619 1631 1665
Size: 309 699 463 148 12 34
Fragments arranged by size:
699 463 309 148 34 12
BbsI GAAGACnn'nnnn_
Cuts at: 0 304 1665
Size: 304 1361
BciVI GTATCCnnnnn_n'
Cuts at: 0 1356 1665
Size: 1356 309
BglI GCCn_nnn'nGGC
Cuts at: 0 436 1665
Size: 436 1229
BglII A'GATC_T
Cuts at: 0 465 1665
Size: 465 1200
BlpI GC'TnA_GC
Cuts at: 0 1007 1665
Size: 1007 658
Bme1580I G_kGCm'C
Cuts at: 0 309 975 1280 1665
Size: 309 666 305 385
Fragments arranged by size:
666 385 309 305
BmrI ACTGGGnnnn_n'
Cuts at: 0 1389 1613 1625 1665
Size: 1389 224 12 40
Fragments arranged by size:
1389 224 40 12
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1344 1665
Size: 1344 321
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 782 1314 1665
Size: 39 743 532 351
Fragments arranged by size:
743 532 351 39
Bpu10I CC'TnA_GC
Cuts at: 0 989 1665
Size: 989 676
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 480 1665
Size: 187 293 1185
Fragments arranged by size:
1185 293 187
BsaI GGTCTCn'nnnn_
Cuts at: 0 1039 1665
Size: 1039 626
BsaWI w'CCGG_w
Cuts at: 0 119 1665
Size: 119 1546
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1285 1315 1665
Size: 1285 30 350
Fragments arranged by size:
1285 350 30
BseYI C'CCAG_C
Cuts at: 0 974 1262 1452 1546 1665
Size: 974 288 190 94 119
Fragments arranged by size:
974 288 190 119 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 866 1665
Size: 866 799
BsiHKAI G_wGCw'C
Cuts at: 0 1471 1665
Size: 1471 194
Bsp1286I G_dGCh'C
Cuts at: 0 309 975 1008 1280 1471 1619 1631 1665
Size: 309 666 33 272 191 148 12 34
Fragments arranged by size:
666 309 272 191 148 34 33 12
BspHI T'CATG_A
Cuts at: 0 330 1665
Size: 330 1335
BsrDI GCAATG_nn'
Cuts at: 0 344 911 1665
Size: 344 567 754
Fragments arranged by size:
754 567 344
BsrFI r'CCGG_y
Cuts at: 0 1657 1665
Size: 1657 8
BssSI C'ACGA_G
Cuts at: 0 619 1176 1665
Size: 619 557 489
Fragments arranged by size:
619 557 489
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1495 1665
Size: 1495 170
BstYI r'GATC_y
Cuts at: 0 465 582 1665
Size: 465 117 1083
Fragments arranged by size:
1083 465 117
Bsu36I CC'TnA_GG
Cuts at: 0 891 1665
Size: 891 774
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 999 1665
Size: 999 666
BtsI GCAGTG_nn'
Cuts at: 0 84 541 875 1665
Size: 84 457 334 790
Fragments arranged by size:
790 457 334 84
DraI TTT'AAA
Cuts at: 0 105 1665
Size: 105 1560
EaeI y'GGCC_r
Cuts at: 0 416 1060 1070 1665
Size: 416 644 10 595
Fragments arranged by size:
644 595 416 10
EarI CTCTTCn'nnn_
Cuts at: 0 563 1523 1553 1665
Size: 563 960 30 112
Fragments arranged by size:
960 563 112 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1073 1259 1665
Size: 1073 186 406
Fragments arranged by size:
1073 406 186
EcoICRI GAG'CTC
Cuts at: 0 1469 1665
Size: 1469 196
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 39 295 782 1314 1665
Size: 39 256 487 532 351
Fragments arranged by size:
532 487 351 256 39
EcoO109I rG'GnC_Cy
Cuts at: 0 305 306 1144 1665
Size: 305 1 838 521
Fragments arranged by size:
838 521 305 1
HaeII r_GCGC'y
Cuts at: 0 44 224 914 1665
Size: 44 180 690 751
Fragments arranged by size:
751 690 180 44
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 760 792 1285 1317 1665
Size: 760 32 493 32 348
Fragments arranged by size:
760 493 348 32 32
HincII GTy'rAC
Cuts at: 0 5 712 1665
Size: 5 707 953
Fragments arranged by size:
953 707 5
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 975 1471 1619 1665
Size: 975 496 148 46
Fragments arranged by size:
975 496 148 46
MscI TGG'CCA
Cuts at: 0 418 1062 1665
Size: 418 644 603
Fragments arranged by size:
644 603 418
MslI CAynn'nnrTG
Cuts at: 0 320 1499 1665
Size: 320 1179 166
Fragments arranged by size:
1179 320 166
MspA1I CmG'CkG
Cuts at: 0 1129 1665
Size: 1129 536
NspI r_CATG'y
Cuts at: 0 756 1665
Size: 756 909
PciI A'CATG_T
Cuts at: 0 752 1665
Size: 752 913
PflMI CCAn_nnn'nTGG
Cuts at: 0 1019 1665
Size: 1019 646
PpuMI rG'GwC_Cy
Cuts at: 0 1144 1665
Size: 1144 521
PspOMI G'GGCC_C
Cuts at: 0 305 1665
Size: 305 1360
PvuII CAG'CTG
Cuts at: 0 1129 1665
Size: 1129 536
SacI G_AGCT'C
Cuts at: 0 1471 1665
Size: 1471 194
SapI GCTCTTCn'nnn_
Cuts at: 0 1553 1665
Size: 1553 112
SexAI A'CCwGG_T
Cuts at: 0 1189 1665
Size: 1189 476
SmlI C'TyrA_G
Cuts at: 0 202 459 1665
Size: 202 257 1206
Fragments arranged by size:
1206 257 202
SspI AAT'ATT
Cuts at: 0 563 1665
Size: 563 1102
StuI AGG'CCT
Cuts at: 0 1324 1588 1665
Size: 1324 264 77
Fragments arranged by size:
1324 264 77
StyI C'CwwG_G
Cuts at: 0 1140 1665
Size: 1140 525
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 958 1222 1665
Size: 958 264 443
Fragments arranged by size:
958 443 264
TatI w'GTAC_w
Cuts at: 0 596 1665
Size: 596 1069
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 520 552 1665
Size: 520 32 1113
Fragments arranged by size:
1113 520 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1224 1665
Size: 1224 441
Enzymes that do cut:
AclI AcuI AflIII AlfI ApaI ApoI AvaI BanI
BanII BbsI BciVI BglI BglII BlpI Bme1580I BmrI
BplI BpmI Bpu10I BpuEI BsaI BsaWI BsaXI BseYI
BsgI BsiHKAI Bsp1286I BspHI BsrDI BsrFI BssSI BstAPI
BstYI Bsu36I BtgZI BtsI DraI EaeI EarI EciI
EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII MmeI MscI
MslI MspA1I NspI PciI PflMI PpuMI PspOMI PvuII
SacI SapI SexAI SmlI SspI StuI StyI TaqII
TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AgeI AhdI
AleI AloI AlwNI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BmgBI
BmtI BsaAI BsaBI BsaHI BseRI BsiEI BsiWI BsmI
BsmBI BspEI BspMI BsrBI BsrGI BssHII BstBI BstEII
BstXI BstZ17I BtgI ClaI CspCI DraIII DrdI EagI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SwaI Tth111I XbaI XhoI XmaI XmnI
ZraI