Restriction map for
pFN21AB8806
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# NO CUT ENZYMES ( 150 enzymes) #
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AatII AccI AccII AclI AcyI
AflII AflIII AgeI AhdI AocI
Aor51HI AscI AseI AsnI Asp700I
AspEI AspI AvaI AviII AvrII
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BcnI BfaI BfrI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmFI
BsmI BsoBI BspDI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HindIII HpaI KasI KspI
MaeI MaeII MamI MfeI MluI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
SacI SacII SalI SbfI ScaI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI Tth111I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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Acc65I 392 GGTACC G^GTACC
AccIII 923 TCCGGA T^CCGGA
AcsI 529 GAATTC [GA]^AATT[CT]
Alw44I 964 GTGCAC G^TGCAC
AlwNI 595 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 964 GTGCAC G^TGCAC
ApoI 529 GAATTC [GA]^AATT[CT]
Asp718I 392 GGTACC G^GTACC
AspHI 964 GTGCAC G[AT]GC[AT]^C
BanI 392 GGTACC G^G[CT][GA]CC
BglII 359 AGATCT A^GATCT
BpmI 1006 CTGGAG CTGGAG(16/14)
BsaWI 923 TCCGGA [AT]^CCGG[AT]
BseAI 923 TCCGGA T^CCGGA
BsiHKAI 964 GTGCAC G[AT]GC[AT]^C
Bsp1407I 350 TGTACA T^GTACA
BspEI 923 TCCGGA T^CCGGA
BsrGI 350 TGTACA T^GTACA
Cac8I 426 GCGGGC GC[ACGT]^[ACGT]GC
CpoI 433 CGGACCG CG^G[AT]CCG
DdeI 679 CTAAG C^T[ACGT]AG
DraIII 409 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 995 CCGTGG C^C[GA][CT]GG
EarI 244 CTCTTC CTCTTC(1/4)
EcoRI 529 GAATTC G^AATTC
EcoT14I 125 CCTTGG C^C[AT][AT]GG
FauI 426 GCGGG CCCGC(4/6)
HapII 924 CCGG C^CGG
HincII 886 GTCAAC GT[CT]^[GA]AC
HindII 886 GTCAAC GT[CT]^[GA]AC
HinfI 672 GATTC G^A[ACGT]TC
HpaII 924 CCGG C^CGG
KpnI 392 GGTACC GGTAC^C
Ksp632I 244 CTCTTC CTCTTC(1/4)
MroI 923 TCCGGA T^CCGGA
MseI 974 TTAA T^TAA
MspI 924 CCGG C^CGG
PstI 546 CTGCAG CTGCA^G
RsrII 433 CGGACCG CG^G[AT]CCG
SapI 243 GCTCTTC GCTCTTC(1/4)
SexAI 968 ACCTGGT A^CC[AT]GGT
SfcI 546 CTGCAG C^T[GA][CT]AG
SfiI 90 GGCCTTTGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 350 TGTACA T^GTACA
StyI 125 CCTTGG C^C[AT][AT]GG
TaqI 272 TCGA T^CGA
TfiI 672 GATTC G^A[AT]TC
Tru9I 974 TTAA T^TAA
TthHB8I 272 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AciI 426 GCGG CCGC(-3/-1)
936 GCGG
AlwI 321 GATCC GGATC(4/5)
269 GGATC
ApaI 98 GGGCCC GGGCC^C
473 GGGCCC
BanII 98 GGGCCC G[GA]GC[CT]^C
473 GGGCCC
BbvI 548 GCAGC GCAGC(8/12)
108 GCTGC
BclI 644 TGATCA T^GATCA
734 TGATCA
BspHI 641 TCATGA T^CATGA
731 TCATGA
BsrI 293 ACTGG ACTGG(1/-1)
892 CCAGT
BstYI 320 AGATCC [GA]^GATC[CT]
359 AGATCT
FbaI 644 TGATCA T^GATCA
734 TGATCA
MaeIII 439 GTCAC ^GT[ACGT]AC
853 GTGAC
MboII 696 GAAGA GAAGA(8/7)
245 TCTTC
MflI 320 AGATCC [GA]^GATC[CT]
359 AGATCT
RcaI 641 TCATGA T^CATGA
731 TCATGA
TseI 108 GCTGC G^C[AT]GC
548 GCAGC
Tsp45I 439 GTCAC ^GT[GC]AC
853 GTGAC
Tsp509I 530 AATT ^AATT
766 AATT
TspRI 877 CACTG CA[GC]TG[ACGT][ACGT]^
893 CAGTG
XhoII 320 AGATCC [GA]^GATC[CT]
359 AGATCT
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AfaI AluI AvaII BalI BglI
BmyI BsaJI BsiYI BslI Bsp1286I
BspMI BssKI BstF5I BstNI Cfr13I
DpnII DraII EaeI EcoO109I EcoRII
Fnu4HI FokI HaeIII HphI ItaI
MboI MluNI MnlI MscI MslI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI