(Circular) (Six-base) MAPSORT of: pFN21AB8806.seq Check: 4616 from: 1 to: 5770 pFN21AB8806 5770 bp With 182 enzymes: SgfI * October 20, 2009 10:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2558 2558 Size: 5770 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3113 1937 Size: 1176 4594 Acc65I G'GTAC_C Cuts at: 2397 3091 2397 Size: 694 5076 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3833 5217 5485 1632 Size: 2201 1384 268 1917 Fragments arranged by size: 2201 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 4669 5646 1203 Size: 3466 977 1327 Fragments arranged by size: 3466 1327 977 AgeI A'CCGG_T Cuts at: 4601 4601 Size: 5770 AhdI GACnn_n'nnGTC Cuts at: 4499 4499 Size: 5770 AleI CACnn'nnGTG Cuts at: 1166 2886 2958 1166 Size: 1720 72 3978 Fragments arranged by size: 3978 1720 72 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2089 1184 Size: 905 4865 AlwNI CAG_nnn'CTG Cuts at: 2605 3513 5085 2605 Size: 908 1572 3290 Fragments arranged by size: 3290 1572 908 ApaI G_GGCC'C Cuts at: 2107 2482 2107 Size: 375 5395 ApaLI G'TGCA_C Cuts at: 2969 3827 4983 2969 Size: 858 1156 3756 Fragments arranged by size: 3756 1156 858 ApoI r'AATT_y Cuts at: 1493 2534 3079 3305 3457 5566 1493 Size: 1041 545 226 152 2109 1697 Fragments arranged by size: 2109 1697 1041 545 226 152 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 3095 1870 Size: 73 1152 4545 Fragments arranged by size: 4545 1152 73 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2397 3091 4546 618 Size: 325 217 627 610 694 1455 1842 Fragments arranged by size: 1842 1455 694 627 610 325 217 BanII G_rGCy'C Cuts at: 1391 2107 2482 3089 1391 Size: 716 375 607 4072 Fragments arranged by size: 4072 716 607 375 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4799 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3961 3995 1903 Size: 34 2024 34 3678 Fragments arranged by size: 3678 2024 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 2649 2739 1548 Size: 1101 90 4579 Fragments arranged by size: 4579 1101 90 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2102 2432 2492 Size: 107 122 71 631 605 430 330 60 Cuts at: 2492 4381 136 Size: 1889 1525 Fragments arranged by size: 1889 1525 631 605 430 330 122 107 71 60 BglII A'GATC_T Cuts at: 2364 5765 2364 Size: 3401 2369 BlpI GC'TnA_GC Cuts at: 3187 3187 Size: 5770 Bme1580I G_kGCm'C Cuts at: 1790 2107 2437 2482 2973 3831 4987 1790 Size: 317 330 45 491 858 1156 2573 Fragments arranged by size: 2573 1156 858 491 330 317 45 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2308 2891 3501 Size: 416 53 83 399 24 891 583 610 Cuts at: 3501 4459 442 Size: 958 1753 Fragments arranged by size: 1753 958 891 610 583 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3032 4430 1239 Size: 1793 1398 2579 Fragments arranged by size: 2579 1793 1398 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3895 4760 5058 3895 Size: 865 298 4607 Fragments arranged by size: 4607 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4433 882 Size: 617 2934 2219 Fragments arranged by size: 2934 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 5764 5764 Size: 5770 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3577 3959 4594 275 Size: 53 83 186 936 2044 382 635 1451 Fragments arranged by size: 2044 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2928 4203 4601 4875 5022 5433 2928 Size: 1275 398 274 147 411 3265 Fragments arranged by size: 3265 1275 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5545 Fragments arranged by size: 5545 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5770 BseYI C'CCAG_C Cuts at: 3062 4973 5469 3062 Size: 1911 496 3363 Fragments arranged by size: 3363 1911 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5197 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3220 3981 5009 1540 Size: 275 186 1219 761 1028 2301 Fragments arranged by size: 2301 1219 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2973 3831 3916 4987 2973 Size: 858 85 1071 3756 Fragments arranged by size: 3756 1071 858 85 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2107 2437 2482 2973 3089 3831 3916 Size: 399 317 330 45 491 116 742 85 Cuts at: 3916 4987 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 491 399 330 317 116 85 45 BspEI T'CCGG_A Cuts at: 2928 5433 2928 Size: 2505 3265 BspHI T'CATG_A Cuts at: 2646 2736 3659 5389 2646 Size: 90 923 1730 3027 Fragments arranged by size: 3027 1730 923 90 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2253 2558 2946 3125 844 Size: 1409 305 388 179 3489 Fragments arranged by size: 3489 1409 388 305 179 BsrBI CCG'CTC Cuts at: 1394 1823 3657 1394 Size: 429 1834 3507 Fragments arranged by size: 3507 1834 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4265 4439 65 Size: 1183 3017 174 1396 Fragments arranged by size: 3017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4414 4601 1700 Size: 110 2604 187 2869 Fragments arranged by size: 2869 2604 187 110 BsrGI T'GTAC_A Cuts at: 96 2355 96 Size: 2259 3511 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5770 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstYI r'GATC_y Cuts at: 1972 2325 2364 3100 3467 3852 3869 5321 5407 Size: 353 39 736 367 385 17 1452 86 Cuts at: 5407 5419 5765 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 736 385 367 353 346 86 39 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3000 513 Size: 552 939 996 3283 Fragments arranged by size: 3283 996 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5031 Fragments arranged by size: 5031 683 56 BtsI GCAGTG_nn' Cuts at: 2881 3286 4080 4100 2881 Size: 405 794 20 4551 Fragments arranged by size: 4551 794 405 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2032 2067 529 Size: 35 1468 35 4232 Fragments arranged by size: 4232 1468 35 35 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DraIII CAC_nnn'GTG Cuts at: 2419 2419 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2232 2403 2484 Size: 54 1425 50 275 18 402 171 81 Cuts at: 2484 3217 4106 5735 8 Size: 733 889 1629 43 Fragments arranged by size: 1629 1425 889 733 402 275 171 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3217 1537 Size: 275 1405 4090 Fragments arranged by size: 4090 1405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2255 3700 1353 Size: 902 1445 3423 Fragments arranged by size: 3423 1445 902 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4357 4731 4877 387 Size: 1064 138 2768 374 146 1280 Fragments arranged by size: 2768 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3032 3833 4430 5217 5485 1239 Size: 393 1400 801 597 787 268 1524 Fragments arranged by size: 1524 1400 801 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2094 2103 2479 2094 Size: 9 376 5385 Fragments arranged by size: 5385 376 9 EcoRI G'AATT_C Cuts at: 2534 3079 3457 2534 Size: 545 378 4847 Fragments arranged by size: 4847 545 378 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 4917 1678 Size: 3239 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2690 2722 4411 4443 4485 Size: 32 135 32 1118 32 1689 32 42 Cuts at: 4485 4517 1373 Size: 32 2626 Fragments arranged by size: 2626 1689 1118 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2893 3114 3360 669 Size: 988 281 955 221 246 3079 Fragments arranged by size: 3079 988 955 281 246 221 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 2401 3095 2401 Size: 694 5076 MfeI C'AATT_G Cuts at: 3369 3369 Size: 5770 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2134 4884 5068 1305 Size: 829 2750 184 2007 Fragments arranged by size: 2750 2007 829 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2234 2405 2486 10 Size: 54 1425 343 402 171 81 3294 Fragments arranged by size: 3294 1425 402 343 171 81 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 2820 2886 2958 3728 4087 4246 Size: 648 999 655 66 72 770 359 159 Cuts at: 4246 518 Size: 2042 Fragments arranged by size: 2042 999 770 655 648 359 159 72 66 MspA1I CmG'CkG Cuts at: 1606 3186 3863 5011 5256 1606 Size: 1580 677 1148 245 2120 Fragments arranged by size: 2120 1580 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4279 Fragments arranged by size: 4279 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 3128 4673 1132 Size: 97 1899 1545 2229 Fragments arranged by size: 2229 1899 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5770 PciI A'CATG_T Cuts at: 4669 4669 Size: 5770 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5770 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5770 PsiI TTA'TAA Cuts at: 2672 3340 2672 Size: 668 5102 PspOMI G'GGCC_C Cuts at: 2103 2478 2103 Size: 375 5395 PstI C_TGCA'G Cuts at: 830 2555 3122 830 Size: 1725 567 3478 Fragments arranged by size: 3478 1725 567 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 RsrII CG'GwC_CG Cuts at: 2439 2439 Size: 5770 SalI G'TCGA_C Cuts at: 1936 3112 1936 Size: 1176 4594 SapI GCTCTTCn'nnn_ Cuts at: 2255 2255 Size: 5770 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 4018 5730 4018 Size: 1712 4058 SexAI A'CCwGG_T Cuts at: 2973 2973 Size: 5770 SfcI C'TryA_G Cuts at: 826 1045 2551 3118 4253 4934 5125 826 Size: 219 1506 567 1135 681 191 1471 Fragments arranged by size: 1506 1471 1135 681 567 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2102 2102 Size: 5770 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 3097 1872 Size: 1225 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3874 4775 5037 820 Size: 197 926 1931 901 262 1553 Fragments arranged by size: 1931 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 3128 1132 Size: 1996 3774 SspI AAT'ATT Cuts at: 5 52 3694 5 Size: 47 3642 2081 Fragments arranged by size: 3642 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5770 StyI C'CwwG_G Cuts at: 513 1065 2004 2130 3209 513 Size: 552 939 126 1079 3074 Fragments arranged by size: 3074 1079 939 552 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2828 3797 3814 3967 4152 4597 325 Size: 1255 1248 969 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1248 969 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2124 2355 4016 Size: 274 80 33 51 1443 147 231 1661 Cuts at: 4016 5728 96 Size: 1712 138 Fragments arranged by size: 1712 1661 1443 274 231 147 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5730 5762 5730 Size: 32 5738 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5770 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 3095 1870 Size: 1225 4545 XmnI GAAnn'nnTTC Cuts at: 3899 3899 Size: 5770 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII SacI SacII SanDI SfoI SrfI SwaI XcmI