(Circular) (Six-base) MAPSORT of: pFN21ASDB0022.seq Check: 628 from: 1 to: 6289 pFN21ASDB0022 6289 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4099 278 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2930 3632 1937 Size: 993 702 4594 Fragments arranged by size: 4594 993 702 Acc65I G'GTAC_C Cuts at: 3610 3610 Size: 6289 AclI AA'CG_TT Cuts at: 1563 4416 4789 1563 Size: 2853 373 3063 Fragments arranged by size: 3063 2853 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4352 5736 6004 1632 Size: 2720 1384 268 1917 Fragments arranged by size: 2720 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6092 AflIII A'CryG_T Cuts at: 1203 5188 6165 1203 Size: 3985 977 1327 Fragments arranged by size: 3985 1327 977 AgeI A'CCGG_T Cuts at: 3346 5120 3346 Size: 1774 4515 AhdI GACnn_n'nnGTC Cuts at: 5018 5018 Size: 6289 AleI CACnn'nnGTG Cuts at: 1166 2951 3294 1166 Size: 1785 343 4161 Fragments arranged by size: 4161 1785 343 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3299 1184 Size: 2115 4174 AlwNI CAG_nnn'CTG Cuts at: 3530 4032 5604 3530 Size: 502 1572 4215 Fragments arranged by size: 4215 1572 502 ApaI G_GGCC'C Cuts at: 3498 3498 Size: 6289 ApaLI G'TGCA_C Cuts at: 4346 5502 4346 Size: 1156 5133 ApoI r'AATT_y Cuts at: 1493 3598 3824 3976 6085 1493 Size: 2105 226 152 2109 1697 Fragments arranged by size: 2109 2105 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4843 160 Size: 872 3811 1606 Fragments arranged by size: 3811 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 AvaI C'yCGr_G Cuts at: 1870 1943 2318 2706 3271 3614 1870 Size: 73 375 388 565 343 4545 Fragments arranged by size: 4545 565 388 375 343 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3561 3594 3561 Size: 33 6256 BamHI G'GATC_C Cuts at: 3449 3619 3986 3449 Size: 170 367 5752 Fragments arranged by size: 5752 367 170 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2458 3610 5065 618 Size: 325 217 627 671 1152 1455 1842 Fragments arranged by size: 1842 1455 1152 671 627 325 217 BanII G_rGCy'C Cuts at: 1391 2325 2354 2394 2403 2480 2812 3054 3498 Size: 934 29 40 9 77 332 242 444 Cuts at: 3498 3545 3608 1391 Size: 47 63 4072 Fragments arranged by size: 4072 934 444 332 242 77 63 47 40 29 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5318 BbvCI CC'TCA_GC Cuts at: 3204 3225 3537 3204 Size: 21 312 5956 Fragments arranged by size: 5956 312 21 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4480 4514 1903 Size: 34 2543 34 3678 Fragments arranged by size: 3678 2543 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4181 5391 4181 Size: 1210 5079 BclI T'GATC_A Cuts at: 1548 1548 Size: 6289 BfrBI ATG'CAT Cuts at: 2869 2869 Size: 6289 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2614 4900 136 Size: 107 122 71 631 605 942 2286 1525 Fragments arranged by size: 2286 1525 942 631 605 122 107 71 BglII A'GATC_T Cuts at: 2893 6284 2893 Size: 3391 2898 BlpI GC'TnA_GC Cuts at: 2126 3544 3706 2126 Size: 1418 162 4709 Fragments arranged by size: 4709 1418 162 Bme1580I G_kGCm'C Cuts at: 1790 2463 3498 4350 5506 1790 Size: 673 1035 852 1156 2573 Fragments arranged by size: 2573 1156 1035 852 673 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6289 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2920 2945 3512 Size: 416 53 83 399 24 1503 25 567 Cuts at: 3512 4020 4978 442 Size: 508 958 1753 Fragments arranged by size: 1753 1503 958 567 508 416 399 83 53 25 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6289 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3073 3073 Size: 6289 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2381 3372 4949 1239 Size: 1142 991 1577 2579 Fragments arranged by size: 2579 1577 1142 991 Bpu10I CC'TnA_GC Cuts at: 3204 3225 3414 3537 3204 Size: 21 189 123 5956 Fragments arranged by size: 5956 189 123 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2207 4414 5279 5577 2207 Size: 2207 865 298 2919 Fragments arranged by size: 2919 2207 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2273 2926 3434 3459 4952 882 Size: 617 774 653 508 25 1493 2219 Fragments arranged by size: 2219 1493 774 653 617 508 25 BsaAI yAC'GTr Cuts at: 493 1204 3292 3586 4080 493 Size: 711 2088 294 494 2702 Fragments arranged by size: 2702 2088 711 494 294 BsaBI GATnn'nnATC Cuts at: 2042 6283 2042 Size: 4241 2048 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4096 4478 5113 275 Size: 53 83 186 936 2563 382 635 1451 Fragments arranged by size: 2563 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3346 4722 5120 5394 5541 5952 3346 Size: 1376 398 274 147 411 3683 Fragments arranged by size: 3683 1376 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2354 2384 1178 Size: 30 165 30 951 30 5083 Fragments arranged by size: 5083 951 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2402 2714 3046 1186 Size: 1216 312 332 4429 Fragments arranged by size: 4429 1216 332 312 BseYI C'CCAG_C Cuts at: 2498 2565 2610 2638 2675 2799 2813 2907 3132 Size: 67 45 28 37 124 14 94 225 Cuts at: 3132 3400 3439 3517 5492 5988 2498 Size: 268 39 78 1975 496 2799 Fragments arranged by size: 2799 1975 496 268 225 124 94 78 67 45 39 37 28 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3066 1163 Size: 573 1330 4386 Fragments arranged by size: 4386 1330 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3739 4500 5528 1540 Size: 275 186 1738 761 1028 2301 Fragments arranged by size: 2301 1738 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2394 2480 2707 3054 4350 4435 5506 2394 Size: 86 227 347 1296 85 1071 3177 Fragments arranged by size: 3177 1296 1071 347 227 86 85 BsmI GAATG_Cn' Cuts at: 3800 3893 3800 Size: 93 6196 BsmBI CGTCTCn'nnnn_ Cuts at: 3452 3452 Size: 6289 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2325 2354 2394 2403 2463 2480 2707 Size: 399 535 29 40 9 60 17 227 Cuts at: 2707 2812 3054 3498 3545 3608 4350 4435 5506 Size: 105 242 444 47 63 742 85 1071 Cuts at: 5506 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 535 444 399 242 227 105 85 63 60 47 40 29 17 9 BspEI T'CCGG_A Cuts at: 5952 5952 Size: 6289 BspHI T'CATG_A Cuts at: 4178 5908 4178 Size: 1730 4559 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2838 3644 844 Size: 1994 806 3489 Fragments arranged by size: 3489 1994 806 BsrBI CCG'CTC Cuts at: 1394 1823 2065 4176 1394 Size: 429 242 2111 3507 Fragments arranged by size: 3507 2111 429 242 BsrDI GCAATG_nn' Cuts at: 65 1248 3106 4784 4958 65 Size: 1183 1858 1678 174 1396 Fragments arranged by size: 1858 1678 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3346 4933 5120 1700 Size: 110 1536 1587 187 2869 Fragments arranged by size: 2869 1587 1536 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6289 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6289 BssSI C'ACGA_G Cuts at: 2249 4349 5361 2249 Size: 2100 1012 3177 Fragments arranged by size: 3177 2100 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6289 BstBI TT'CG_AA Cuts at: 2007 5093 2007 Size: 3086 3203 BstEII G'GTnAC_C Cuts at: 1184 2512 2645 1184 Size: 1328 133 4828 Fragments arranged by size: 4828 1328 133 BstXI CCAn_nnnn'nTGG Cuts at: 2915 2953 2915 Size: 38 6251 BstYI r'GATC_y Cuts at: 1972 2043 2893 3449 3619 3986 4371 4388 5840 Size: 71 850 556 170 367 385 17 1452 Cuts at: 5840 5926 5938 6284 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 850 556 385 367 346 170 86 71 17 12 Bsu36I CC'TnA_GG Cuts at: 2285 3531 2285 Size: 1246 5043 BtgI C'CryG_G Cuts at: 513 1065 2022 2549 513 Size: 552 957 527 4253 Fragments arranged by size: 4253 957 552 527 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3438 487 Size: 683 56 2212 3338 Fragments arranged by size: 3338 2212 683 56 BtsI GCAGTG_nn' Cuts at: 2480 3012 3105 3805 4599 4619 2480 Size: 532 93 700 794 20 4150 Fragments arranged by size: 4150 794 700 532 93 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6289 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6254 DraI TTT'AAA Cuts at: 3593 3593 Size: 6289 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2834 5296 809 Size: 507 1518 2462 1802 Fragments arranged by size: 2462 1802 1518 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2615 2733 3736 Size: 54 1425 50 275 18 785 118 1003 Cuts at: 3736 4625 6254 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1003 889 785 275 118 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3736 1537 Size: 275 1924 4090 Fragments arranged by size: 4090 1924 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2408 3007 3548 4219 1353 Size: 1055 599 541 671 3423 Fragments arranged by size: 3423 1055 671 599 541 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2766 4876 5250 5396 387 Size: 1064 138 1177 2110 374 146 1280 Fragments arranged by size: 2110 1280 1177 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2392 2478 3052 2392 Size: 86 574 5629 Fragments arranged by size: 5629 574 86 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2381 3372 4352 4949 5736 6004 1239 Size: 393 749 991 980 597 787 268 1524 Fragments arranged by size: 1524 991 980 787 749 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2360 2753 2360 Size: 393 5896 EcoO109I rG'GnC_Cy Cuts at: 2989 2989 Size: 6289 EcoRI G'AATT_C Cuts at: 3598 3976 3598 Size: 378 5911 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2314 2314 Size: 6289 FspI TGC'GCA Cuts at: 4795 4795 Size: 6289 HaeII r_GCGC'y Cuts at: 1678 2413 2914 5436 1678 Size: 735 501 2522 2531 Fragments arranged by size: 2531 2522 735 501 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4930 4962 5004 5036 1373 Size: 32 135 32 3358 32 42 32 2626 Fragments arranged by size: 3358 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2421 3039 3633 3879 669 Size: 988 281 483 618 594 246 3079 Fragments arranged by size: 3079 988 618 594 483 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6289 HpaI GTT'AAC Cuts at: 3879 3879 Size: 6289 KpnI G_GTAC'C Cuts at: 3614 3614 Size: 6289 MfeI C'AATT_G Cuts at: 3888 3888 Size: 6289 MluI A'CGCG_T Cuts at: 6165 6165 Size: 6289 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2199 5403 5587 1305 Size: 894 3204 184 2007 Fragments arranged by size: 3204 2007 894 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2735 10 Size: 54 1425 343 903 3564 Fragments arranged by size: 3564 1425 903 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2204 2951 3294 4247 4606 4765 518 Size: 648 1038 747 343 953 359 159 2042 Fragments arranged by size: 2042 1038 953 747 648 359 343 159 MspA1I CmG'CkG Cuts at: 1606 2502 2551 2614 3392 3404 3548 3705 4382 Size: 896 49 63 778 12 144 157 677 Cuts at: 4382 5530 5775 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 896 778 677 245 157 144 63 49 12 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4780 Fragments arranged by size: 4780 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6289 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6289 NotI GC'GGCC_GC Cuts at: 3736 3736 Size: 6289 NsiI A_TGCA'T Cuts at: 2871 2871 Size: 6289 NspI r_CATG'y Cuts at: 1132 1229 3647 5192 1132 Size: 97 2418 1545 2229 Fragments arranged by size: 2418 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6289 PciI A'CATG_T Cuts at: 5188 5188 Size: 6289 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6289 PmeI GTTT'AAAC Cuts at: 3593 3593 Size: 6289 PmlI CAC'GTG Cuts at: 3292 3292 Size: 6289 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4358 4390 5871 5903 4358 Size: 32 1481 32 4744 Fragments arranged by size: 4744 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6289 PsiI TTA'TAA Cuts at: 3859 3859 Size: 6289 PspOMI G'GGCC_C Cuts at: 3494 3494 Size: 6289 PstI C_TGCA'G Cuts at: 830 2487 3641 830 Size: 1657 1154 3478 Fragments arranged by size: 3478 1657 1154 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6289 PvuII CAG'CTG Cuts at: 3392 3404 3548 3392 Size: 12 144 6133 Fragments arranged by size: 6133 144 12 SacI G_AGCT'C Cuts at: 2394 2480 3054 2394 Size: 86 574 5629 Fragments arranged by size: 5629 574 86 SacII CC_GC'GG Cuts at: 2552 2552 Size: 6289 SalI G'TCGA_C Cuts at: 1936 3631 1936 Size: 1695 4594 SapI GCTCTTCn'nnn_ Cuts at: 2408 2408 Size: 6289 SbfI CC_TGCA'GG Cuts at: 3641 3641 Size: 6289 ScaI AGT'ACT Cuts at: 4537 6249 4537 Size: 1712 4577 SexAI A'CCwGG_T Cuts at: 3240 3240 Size: 6289 SfcI C'TryA_G Cuts at: 826 1045 2483 2579 3637 4772 5453 5644 826 Size: 219 1438 96 1058 1135 681 191 1471 Fragments arranged by size: 1471 1438 1135 1058 681 219 191 96 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6289 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6289 SmaI CCC'GGG Cuts at: 1872 3273 3616 1872 Size: 1401 343 4545 Fragments arranged by size: 4545 1401 343 SmlI C'TyrA_G Cuts at: 820 1017 1943 2222 4393 5294 5556 820 Size: 197 926 279 2171 901 262 1553 Fragments arranged by size: 2171 1553 926 901 279 262 197 SnaBI TAC'GTA Cuts at: 493 3586 4080 493 Size: 3093 494 2702 Fragments arranged by size: 3093 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6289 SphI G_CATG'C Cuts at: 1132 3647 1132 Size: 2515 3774 SspI AAT'ATT Cuts at: 5 52 4213 5 Size: 47 4161 2081 Fragments arranged by size: 4161 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6289 StyI C'CwwG_G Cuts at: 513 1065 2022 2914 3728 513 Size: 552 957 892 814 3074 Fragments arranged by size: 3074 957 892 814 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2813 3284 3332 4316 4333 4486 4671 Size: 1255 1233 471 48 984 17 153 185 Cuts at: 4671 5116 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1233 984 471 445 185 153 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2226 2235 4535 Size: 274 80 33 51 1443 249 9 2300 Cuts at: 4535 6247 96 Size: 1712 138 Fragments arranged by size: 2300 1712 1443 274 249 138 80 51 33 9 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6249 6281 6249 Size: 32 6257 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6289 XbaI T'CTAG_A Cuts at: 3625 3625 Size: 6289 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2203 2506 2203 Size: 303 5986 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6289 XmaI C'CCGG_G Cuts at: 1870 3271 3614 1870 Size: 1401 343 4545 Fragments arranged by size: 4545 1401 343 XmnI GAAnn'nnTTC Cuts at: 2297 4418 2297 Size: 2121 4168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4097 276 Size: 53 83 186 936 2563 2468 Fragments arranged by size: 2563 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PpuMI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI