(Circular) (Six-base) MAPSORT of: pFN21ASDB0002.seq Check: 598 from: 1 to: 8455 pFN21ASDB0002 8455 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2728 2728 Size: 8455 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6265 278 Size: 53 83 186 936 4729 2468 Fragments arranged by size: 4729 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5798 1937 Size: 3861 4594 Acc65I G'GTAC_C Cuts at: 2340 5776 2340 Size: 3436 5019 AclI AA'CG_TT Cuts at: 1563 6582 6955 1563 Size: 5019 373 3063 Fragments arranged by size: 5019 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2546 6518 7902 8170 1632 Size: 914 3972 1384 268 1917 Fragments arranged by size: 3972 1917 1384 914 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8258 AflIII A'CryG_T Cuts at: 1203 4224 7354 8331 1203 Size: 3021 3130 977 1327 Fragments arranged by size: 3130 3021 1327 977 AgeI A'CCGG_T Cuts at: 7286 7286 Size: 8455 AhdI GACnn_n'nnGTC Cuts at: 7184 7184 Size: 8455 AleI CACnn'nnGTG Cuts at: 1166 2367 5243 5302 5357 1166 Size: 1201 2876 59 55 4264 Fragments arranged by size: 4264 2876 1201 59 55 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2385 5387 1184 Size: 1201 3002 4252 Fragments arranged by size: 4252 3002 1201 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3566 3598 3566 Size: 32 8423 AlwNI CAG_nnn'CTG Cuts at: 2661 3327 4531 5144 6198 7770 2661 Size: 666 1204 613 1054 1572 3346 Fragments arranged by size: 3346 1572 1204 1054 666 613 ApaI G_GGCC'C Cuts at: 2144 2721 3441 4645 4922 5210 2144 Size: 577 720 1204 277 288 5389 Fragments arranged by size: 5389 1204 720 577 288 277 ApaLI G'TGCA_C Cuts at: 5605 6512 7668 5605 Size: 907 1156 6392 Fragments arranged by size: 6392 1156 907 ApoI r'AATT_y Cuts at: 1493 4721 5764 5990 6142 8251 1493 Size: 3228 1043 226 152 2109 1697 Fragments arranged by size: 3228 2109 1697 1043 226 152 AscI GG'CGCG_CC Cuts at: 2147 2147 Size: 8455 AseI AT'TA_AT Cuts at: 160 1032 7009 160 Size: 872 5977 1606 Fragments arranged by size: 5977 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8455 AvaI C'yCGr_G Cuts at: 1870 1943 2153 2286 2402 2720 3480 4352 4638 Size: 73 210 133 116 318 760 872 286 Cuts at: 4638 4644 4699 4931 4959 5241 5355 5780 1870 Size: 6 55 232 28 282 114 425 4545 Fragments arranged by size: 4545 872 760 425 318 286 282 232 210 133 116 114 73 55 28 6 BamHI G'GATC_C Cuts at: 4615 5785 6152 4615 Size: 1170 367 6918 Fragments arranged by size: 6918 1170 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2340 2880 3023 3813 4370 Size: 325 217 627 553 540 143 790 557 Cuts at: 4370 5304 5776 7231 618 Size: 934 472 1455 1842 Fragments arranged by size: 1842 1455 934 790 627 557 553 540 472 325 217 143 BanII G_rGCy'C Cuts at: 1391 2033 2144 2721 3441 3932 4503 4645 4692 Size: 642 111 577 720 491 571 142 47 Cuts at: 4692 4700 4922 5210 5500 5774 1391 Size: 8 222 288 290 274 4072 Fragments arranged by size: 4072 720 642 577 571 491 290 288 274 222 142 111 47 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3641 928 Size: 971 1742 5742 Fragments arranged by size: 5742 1742 971 BbvCI CC'TCA_GC Cuts at: 5134 5474 5134 Size: 340 8115 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2282 2316 2375 2409 4882 4916 6646 Size: 34 345 34 59 34 2473 34 1730 Cuts at: 6646 6680 1903 Size: 34 3678 Fragments arranged by size: 3678 2473 1730 345 59 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4472 6347 7557 4472 Size: 1875 1210 5370 Fragments arranged by size: 5370 1875 1210 BclI T'GATC_A Cuts at: 1548 4732 1548 Size: 3184 5271 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2132 2309 2405 Size: 107 122 71 631 605 460 177 96 Cuts at: 2405 2795 3903 4600 5114 7066 136 Size: 390 1108 697 514 1952 1525 Fragments arranged by size: 1952 1525 1108 697 631 605 514 460 390 177 122 107 96 71 BglII A'GATC_T Cuts at: 3568 8450 3568 Size: 4882 3573 BlpI GC'TnA_GC Cuts at: 3407 5872 3407 Size: 2465 5990 Bme1580I G_kGCm'C Cuts at: 1790 2144 2487 2721 3441 3527 3756 3816 3891 Size: 354 343 234 720 86 229 60 75 Cuts at: 3891 4375 4645 4922 4971 5210 5376 5597 5609 Size: 484 270 277 49 239 166 221 12 Cuts at: 5609 6516 7672 1790 Size: 907 1156 2573 Fragments arranged by size: 2573 1156 907 720 484 354 343 277 270 239 234 229 221 166 86 75 60 49 12 BmgBI CAC'GTC Cuts at: 1318 2059 2482 1318 Size: 741 423 7291 Fragments arranged by size: 7291 741 423 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3640 3878 3885 Size: 416 53 83 399 24 2223 238 7 Cuts at: 3885 4166 4905 4925 5166 5340 6186 7144 442 Size: 281 739 20 241 174 846 958 1753 Fragments arranged by size: 2223 1753 958 846 739 416 399 281 241 238 174 83 53 24 20 7 BmtI G_CTAG'C Cuts at: 1056 4944 1056 Size: 3888 4567 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3334 5350 5689 3334 Size: 2016 339 6100 Fragments arranged by size: 6100 2016 339 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2804 2992 3304 4517 4668 4787 7115 1239 Size: 1565 188 312 1213 151 119 2328 2579 Fragments arranged by size: 2579 2328 1565 1213 312 188 151 119 Bpu10I CC'TnA_GC Cuts at: 2373 5134 5474 2373 Size: 2761 340 5354 Fragments arranged by size: 5354 2761 340 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2240 2488 5148 5671 6580 7445 7743 2240 Size: 248 2660 523 909 865 298 2952 Fragments arranged by size: 2952 2660 909 865 523 298 248 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2768 7118 882 Size: 617 1269 4350 2219 Fragments arranged by size: 4350 2219 1269 617 BsaAI yAC'GTr Cuts at: 493 1204 5026 5752 6246 493 Size: 711 3822 726 494 2702 Fragments arranged by size: 3822 2702 726 711 494 BsaBI GATnn'nnATC Cuts at: 2564 2651 8449 2564 Size: 87 5798 2570 Fragments arranged by size: 5798 2570 87 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3976 6262 6644 7279 Size: 53 83 186 936 2443 2286 382 635 Cuts at: 7279 275 Size: 1451 Fragments arranged by size: 2443 2286 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2035 3336 3361 6888 7286 7560 7707 8118 2035 Size: 1301 25 3527 398 274 147 411 2372 Fragments arranged by size: 3527 2372 1301 411 398 274 147 25 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3125 3155 3725 3755 1178 Size: 30 165 30 1722 30 570 30 5878 Fragments arranged by size: 5878 1722 570 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3292 3413 3416 3683 3788 4379 4853 5102 Size: 2106 121 3 267 105 591 474 249 Cuts at: 5102 5116 5326 1186 Size: 14 210 4315 Fragments arranged by size: 4315 2106 591 474 267 249 210 121 105 14 3 BseYI C'CCAG_C Cuts at: 2135 2456 2903 3037 3068 3281 3542 4022 4058 Size: 321 447 134 31 213 261 480 36 Cuts at: 4058 4290 4344 4502 4797 4970 5200 5531 7658 Size: 232 54 158 295 173 230 331 2127 Cuts at: 7658 8154 2135 Size: 496 2436 Fragments arranged by size: 2436 2127 496 480 447 331 321 295 261 232 230 213 173 158 134 54 36 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2345 3101 3487 4661 4908 5310 1163 Size: 573 609 756 386 1174 247 402 4308 Fragments arranged by size: 4308 1174 756 609 573 402 386 247 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2631 5905 6666 7694 1540 Size: 275 186 630 3274 761 1028 2301 Fragments arranged by size: 3274 2301 1028 761 630 275 186 BsiHKAI G_wGCw'C Cuts at: 2298 4895 5609 6516 6601 7672 2298 Size: 2597 714 907 85 1071 3081 Fragments arranged by size: 3081 2597 1071 907 714 85 BsmI GAATG_Cn' Cuts at: 2498 5966 6059 2498 Size: 3468 93 4894 Fragments arranged by size: 4894 3468 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2033 2144 2298 2487 2721 3441 3527 Size: 399 243 111 154 189 234 720 86 Cuts at: 3527 3756 3816 3891 3932 4375 4503 4645 4692 Size: 229 60 75 41 443 128 142 47 Cuts at: 4692 4700 4895 4922 4971 5210 5376 5500 5597 Size: 8 195 27 49 239 166 124 97 Cuts at: 5597 5609 5774 6516 6601 7672 1391 Size: 12 165 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 720 443 399 243 239 234 229 195 189 166 165 154 142 128 124 111 97 86 85 75 60 49 47 41 27 12 8 BspEI T'CCGG_A Cuts at: 2035 3336 3361 8118 2035 Size: 1301 25 4757 2372 Fragments arranged by size: 4757 2372 1301 25 BspHI T'CATG_A Cuts at: 6344 8074 6344 Size: 1730 6725 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2085 2728 5810 844 Size: 1241 643 3082 3489 Fragments arranged by size: 3489 3082 1241 643 BsrBI CCG'CTC Cuts at: 1394 1823 3127 3141 3762 5384 5685 6342 1394 Size: 429 1304 14 621 1622 301 657 3507 Fragments arranged by size: 3507 1622 1304 657 621 429 301 14 BsrDI GCAATG_nn' Cuts at: 65 1248 3496 4319 5076 6950 7124 65 Size: 1183 2248 823 757 1874 174 1396 Fragments arranged by size: 2248 1874 1396 1183 823 757 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2877 3093 3154 5307 7099 7286 1700 Size: 110 1067 216 61 2153 1792 187 2869 Fragments arranged by size: 2869 2153 1792 1067 216 187 110 61 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8455 BssHII G'CGCG_C Cuts at: 1925 2119 2147 1925 Size: 194 28 8233 Fragments arranged by size: 8233 194 28 BssSI C'ACGA_G Cuts at: 2771 3526 4597 6515 7527 2771 Size: 755 1071 1918 1012 3699 Fragments arranged by size: 3699 1918 1071 1012 755 BstAPI GCAn_nnn'nTGC Cuts at: 794 3560 4672 5144 794 Size: 2766 1112 472 4105 Fragments arranged by size: 4105 2766 1112 472 BstBI TT'CG_AA Cuts at: 2007 7259 2007 Size: 5252 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8455 BstXI CCAn_nnnn'nTGG Cuts at: 2539 2911 2971 5359 2539 Size: 372 60 2388 5635 Fragments arranged by size: 5635 2388 372 60 BstYI r'GATC_y Cuts at: 1972 3568 4328 4615 5785 6152 6537 6554 8006 Size: 1596 760 287 1170 367 385 17 1452 Cuts at: 8006 8092 8104 8450 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1596 1452 1170 760 385 367 346 287 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4281 5598 4281 Size: 1317 7138 BtgI C'CryG_G Cuts at: 513 1065 2022 2631 3220 3248 3391 5112 513 Size: 552 957 609 589 28 143 1721 3856 Fragments arranged by size: 3856 1721 957 609 589 552 143 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2058 2290 3300 487 Size: 683 56 832 232 1010 5642 Fragments arranged by size: 5642 1010 832 683 232 56 BtsI GCAGTG_nn' Cuts at: 3358 3718 3913 4971 5971 6765 6785 3358 Size: 360 195 1058 1000 794 20 5028 Fragments arranged by size: 5028 1058 1000 794 360 195 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8455 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 5503 5538 529 Size: 35 4939 35 3446 Fragments arranged by size: 4939 3446 35 35 DraI TTT'AAA Cuts at: 5759 5759 Size: 8455 DraIII CAC_nnn'GTG Cuts at: 2272 5348 2272 Size: 3076 5379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7462 809 Size: 507 6146 1802 Fragments arranged by size: 6146 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 2628 2665 Size: 54 1425 50 275 18 294 504 37 Cuts at: 2665 2961 3177 3217 3493 3895 5902 6791 8420 Size: 296 216 40 276 402 2007 889 1629 Cuts at: 8420 8 Size: 43 Fragments arranged by size: 2007 1629 1425 889 504 402 296 294 276 275 216 54 50 43 40 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2628 5902 1537 Size: 275 816 3274 4090 Fragments arranged by size: 4090 3274 816 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3248 4453 4818 6385 1353 Size: 1895 1205 365 1567 3423 Fragments arranged by size: 3423 1895 1567 1205 365 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2327 2804 3733 4379 5326 7042 Size: 1064 138 738 477 929 646 947 1716 Cuts at: 7042 7416 7562 387 Size: 374 146 1280 Fragments arranged by size: 1716 1280 1064 947 929 738 646 477 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2546 2804 2992 3304 4517 4668 4787 Size: 393 914 258 188 312 1213 151 119 Cuts at: 4787 6518 7115 7902 8170 1239 Size: 1731 597 787 268 1524 Fragments arranged by size: 1731 1524 1213 914 787 597 393 312 268 258 188 151 119 EcoNI CCTnn'n_nnAGG Cuts at: 2450 4206 2450 Size: 1756 6699 EcoO109I rG'GnC_Cy Cuts at: 2140 2492 2536 2717 3437 3438 4285 4919 5206 Size: 352 44 181 720 1 847 634 287 Cuts at: 5206 5216 2140 Size: 10 5379 Fragments arranged by size: 5379 847 720 634 352 287 181 44 10 1 EcoRI G'AATT_C Cuts at: 5764 6142 5764 Size: 378 8077 FspI TGC'GCA Cuts at: 2063 2081 6961 2063 Size: 18 4880 3557 Fragments arranged by size: 4880 3557 18 HaeII r_GCGC'y Cuts at: 1678 2199 2662 3111 4351 5235 5706 7602 1678 Size: 521 463 449 1240 884 471 1896 2531 Fragments arranged by size: 2531 1896 1240 884 521 471 463 449 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2036 2068 3949 3981 4430 Size: 32 135 32 464 32 1881 32 449 Cuts at: 4430 4462 4993 5025 5146 5178 7096 7128 7170 Size: 32 531 32 121 32 1918 32 42 Cuts at: 7170 7202 1373 Size: 32 2626 Fragments arranged by size: 2626 1918 1881 531 464 449 135 121 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5799 6045 669 Size: 988 281 3861 246 3079 Fragments arranged by size: 3861 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 8455 HpaI GTT'AAC Cuts at: 6045 6045 Size: 8455 KpnI G_GTAC'C Cuts at: 2344 5780 2344 Size: 3436 5019 MfeI C'AATT_G Cuts at: 6054 6054 Size: 8455 MluI A'CGCG_T Cuts at: 8331 8331 Size: 8455 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2868 4269 4287 7569 7753 1305 Size: 1563 1401 18 3282 184 2007 Fragments arranged by size: 3282 2007 1563 1401 184 18 MscI TGG'CCA Cuts at: 10 64 1489 1832 2667 3219 3495 10 Size: 54 1425 343 835 552 276 4970 Fragments arranged by size: 4970 1425 835 552 343 276 54 MslI CAynn'nnrTG Cuts at: 518 1166 2367 2693 3149 3680 3846 3863 4013 Size: 648 1201 326 456 531 166 17 150 Cuts at: 4013 4146 4223 4850 4994 5000 5243 5302 5357 Size: 133 77 627 144 6 243 59 55 Cuts at: 5357 6413 6772 6931 518 Size: 1056 359 159 2042 Fragments arranged by size: 2042 1201 1056 648 627 531 456 359 326 243 166 159 150 144 133 77 59 55 17 6 MspA1I CmG'CkG Cuts at: 1606 2337 2359 2427 2433 2627 2644 3165 3393 Size: 731 22 68 6 194 17 521 228 Cuts at: 3393 3501 4026 4185 4565 5042 5661 5871 6548 Size: 108 525 159 380 477 619 210 677 Cuts at: 6548 7696 7941 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 731 677 619 525 521 477 380 245 228 210 194 159 108 68 22 17 6 NaeI GCC'GGC Cuts at: 2879 3156 5309 2879 Size: 277 2153 6025 Fragments arranged by size: 6025 2153 277 NcoI C'CATG_G Cuts at: 513 1065 2022 3220 5112 513 Size: 552 957 1198 1892 3856 Fragments arranged by size: 3856 1892 1198 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8455 NgoMIV G'CCGG_C Cuts at: 2877 3154 5307 2877 Size: 277 2153 6025 Fragments arranged by size: 6025 2153 277 NheI G'CTAG_C Cuts at: 1052 4940 1052 Size: 3888 4567 NotI GC'GGCC_GC Cuts at: 5902 5902 Size: 8455 NspI r_CATG'y Cuts at: 1132 1229 2098 2770 3862 4228 5068 5813 7358 Size: 97 869 672 1092 366 840 745 1545 Cuts at: 7358 1132 Size: 2229 Fragments arranged by size: 2229 1545 1092 869 840 745 672 366 97 PasI CC'CwG_GG Cuts at: 1348 3370 3872 4609 5103 5653 1348 Size: 2022 502 737 494 550 4150 Fragments arranged by size: 4150 2022 737 550 502 494 PciI A'CATG_T Cuts at: 4224 7354 4224 Size: 3130 5325 PflMI CCAn_nnn'nTGG Cuts at: 1108 3707 3991 1108 Size: 2599 284 5572 Fragments arranged by size: 5572 2599 284 PfoI T'CCnGG_A Cuts at: 2781 4094 2781 Size: 1313 7142 PmeI GTTT'AAAC Cuts at: 5759 5759 Size: 8455 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6524 6556 8037 8069 6524 Size: 32 1481 32 6910 Fragments arranged by size: 6910 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2492 4285 5216 2492 Size: 1793 931 5731 Fragments arranged by size: 5731 1793 931 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8455 PsiI TTA'TAA Cuts at: 6025 6025 Size: 8455 PspOMI G'GGCC_C Cuts at: 2140 2717 3437 4641 4918 5206 2140 Size: 577 720 1204 277 288 5389 Fragments arranged by size: 5389 1204 720 577 288 277 PspXI vC'TCGA_Gb Cuts at: 2153 2153 Size: 8455 PstI C_TGCA'G Cuts at: 830 2432 2643 3568 4536 5086 5807 830 Size: 1602 211 925 968 550 721 3478 Fragments arranged by size: 3478 1602 968 925 721 550 211 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8455 PvuII CAG'CTG Cuts at: 2337 2359 2427 2644 4565 5042 2337 Size: 22 68 217 1921 477 5750 Fragments arranged by size: 5750 1921 477 217 68 22 SacII CC_GC'GG Cuts at: 3394 3394 Size: 8455 SalI G'TCGA_C Cuts at: 1936 5797 1936 Size: 3861 4594 SbfI CC_TGCA'GG Cuts at: 5807 5807 Size: 8455 ScaI AGT'ACT Cuts at: 2159 6703 8415 2159 Size: 4544 1712 2199 Fragments arranged by size: 4544 2199 1712 SexAI A'CCwGG_T Cuts at: 2268 2268 Size: 8455 SfcI C'TryA_G Cuts at: 826 1045 2354 2428 2639 3564 4532 5082 5803 Size: 219 1309 74 211 925 968 550 721 Cuts at: 5803 6938 7619 7810 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1309 1135 968 925 721 681 550 219 211 191 74 SfiI GGCCn_nnn'nGGCC Cuts at: 2795 2795 Size: 8455 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8455 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8455 SmaI CCC'GGG Cuts at: 1872 2288 2722 4354 4701 4933 4961 5243 5357 Size: 416 434 1632 347 232 28 282 114 Cuts at: 5357 5782 1872 Size: 425 4545 Fragments arranged by size: 4545 1632 434 425 416 347 282 232 114 28 SmlI C'TyrA_G Cuts at: 820 1017 1943 2153 2219 2467 5163 5686 6559 Size: 197 926 210 66 248 2696 523 873 Cuts at: 6559 7460 7722 820 Size: 901 262 1553 Fragments arranged by size: 2696 1553 926 901 873 523 262 248 210 197 66 SnaBI TAC'GTA Cuts at: 493 5752 6246 493 Size: 5259 494 2702 Fragments arranged by size: 5259 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8455 SphI G_CATG'C Cuts at: 1132 2098 5813 1132 Size: 966 3715 3774 Fragments arranged by size: 3774 3715 966 SspI AAT'ATT Cuts at: 5 52 4717 6379 5 Size: 47 4665 1662 2081 Fragments arranged by size: 4665 2081 1662 47 StuI AGG'CCT Cuts at: 1517 4954 5479 1517 Size: 3437 525 4493 Fragments arranged by size: 4493 3437 525 StyI C'CwwG_G Cuts at: 513 1065 2022 2446 2532 3220 3537 3795 4620 Size: 552 957 424 86 688 317 258 825 Cuts at: 4620 5112 5502 5894 513 Size: 492 390 392 3074 Fragments arranged by size: 3074 957 825 688 552 492 424 392 390 317 258 86 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3416 4358 4460 5359 6482 6499 6652 Size: 1255 1836 942 102 899 1123 17 153 Cuts at: 6652 6837 7282 325 Size: 185 445 1498 Fragments arranged by size: 1836 1498 1255 1123 942 899 445 185 153 102 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2157 3672 4842 Size: 274 80 33 51 1443 180 1515 1170 Cuts at: 4842 6701 8413 96 Size: 1859 1712 138 Fragments arranged by size: 1859 1712 1515 1443 1170 274 180 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3125 3157 3725 3757 8415 8447 3125 Size: 32 568 32 4658 32 3133 Fragments arranged by size: 4658 3133 568 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3640 1316 Size: 2324 6131 XbaI T'CTAG_A Cuts at: 5791 5791 Size: 8455 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3848 4171 4582 5110 3848 Size: 323 411 528 7193 Fragments arranged by size: 7193 528 411 323 XhoI C'TCGA_G Cuts at: 1943 2153 1943 Size: 210 8245 XmaI C'CCGG_G Cuts at: 1870 2286 2720 4352 4699 4931 4959 5241 5355 Size: 416 434 1632 347 232 28 282 114 Cuts at: 5355 5780 1870 Size: 425 4545 Fragments arranged by size: 4545 1632 434 425 416 347 282 232 114 28 XmnI GAAnn'nnTTC Cuts at: 6584 6584 Size: 8455 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6263 276 Size: 53 83 186 936 4729 2468 Fragments arranged by size: 4729 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI RsrII SacI SanDI SapI SfoI SrfI SwaI