Restriction map for
pFN21ASDB0002
(Linear) (Six-base) MAPSORT of: KIBB0002.seq Check: 4987 from: 1 to: 3726
KIBB0002.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 705 3726
Size: 705 3021
Acc65I G'GTAC_C
Cuts at: 0 317 3726
Size: 317 3409
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 3726
Size: 523 3203
AflIII A'CryG_T
Cuts at: 0 2201 3726
Size: 2201 1525
AleI CACnn'nnGTG
Cuts at: 0 344 3220 3279 3334 3726
Size: 344 2876 59 55 392
Fragments arranged by size:
2876 392 344 59 55
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 362 3364 3726
Size: 362 3002 362
Fragments arranged by size:
3002 362 362
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1543 1575 3726
Size: 1543 32 2151
Fragments arranged by size:
2151 1543 32
AlwNI CAG_nnn'CTG
Cuts at: 0 638 1304 2508 3121 3726
Size: 638 666 1204 613 605
Fragments arranged by size:
1204 666 638 613 605
ApaI G_GGCC'C
Cuts at: 0 121 698 1418 2622 2899 3187 3726
Size: 121 577 720 1204 277 288 539
Fragments arranged by size:
1204 720 577 539 288 277 121
ApaLI G'TGCA_C
Cuts at: 0 3582 3726
Size: 3582 144
ApoI r'AATT_y
Cuts at: 0 2698 3726
Size: 2698 1028
AscI GG'CGCG_CC
Cuts at: 0 124 3726
Size: 124 3602
AvaI C'yCGr_G
Cuts at: 0 130 263 379 697 1457 2329 2615 2621
Size: 130 133 116 318 760 872 286 6
Cuts at: 2621 2676 2908 2936 3218 3332 3726
Size: 55 232 28 282 114 394
Fragments arranged by size:
872 760 394 318 286 282 232 133
130 116 114 55 28 6
BamHI G'GATC_C
Cuts at: 0 2592 3726
Size: 2592 1134
BanI G'GyrC_C
Cuts at: 0 317 857 1000 1790 2347 3281 3726
Size: 317 540 143 790 557 934 445
Fragments arranged by size:
934 790 557 540 445 317 143
BanII G_rGCy'C
Cuts at: 0 10 121 698 1418 1909 2480 2622 2669
Size: 10 111 577 720 491 571 142 47
Cuts at: 2669 2677 2899 3187 3477 3726
Size: 8 222 288 290 249
Fragments arranged by size:
720 577 571 491 290 288 249 222
142 111 47 10 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 1618 3726
Size: 1618 2108
BbvCI CC'TCA_GC
Cuts at: 0 3111 3451 3726
Size: 3111 340 275
Fragments arranged by size:
3111 340 275
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 259 293 352 386 2859 2893 3726
Size: 259 34 59 34 2473 34 833
Fragments arranged by size:
2473 833 259 59 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2449 3726
Size: 2449 1277
BclI T'GATC_A
Cuts at: 0 2709 3726
Size: 2709 1017
BglI GCCn_nnn'nGGC
Cuts at: 0 109 286 382 772 1880 2577 3091 3726
Size: 109 177 96 390 1108 697 514 635
Fragments arranged by size:
1108 697 635 514 390 177 109 96
BglII A'GATC_T
Cuts at: 0 1545 3726
Size: 1545 2181
BlpI GC'TnA_GC
Cuts at: 0 1384 3726
Size: 1384 2342
Bme1580I G_kGCm'C
Cuts at: 0 121 464 698 1418 1504 1733 1793 1868
Size: 121 343 234 720 86 229 60 75
Cuts at: 1868 2352 2622 2899 2948 3187 3353 3574 3586
Size: 484 270 277 49 239 166 221 12
Cuts at: 3586 3726
Size: 140
Fragments arranged by size:
720 484 343 277 270 239 234 229
221 166 140 121 86 75 60 49
12
BmgBI CAC'GTC
Cuts at: 0 36 459 3726
Size: 36 423 3267
Fragments arranged by size:
3267 423 36
BmrI ACTGGGnnnn_n'
Cuts at: 0 1617 1855 1862 2143 2882 2902 3143 3317
Size: 1617 238 7 281 739 20 241 174
Cuts at: 3317 3726
Size: 409
Fragments arranged by size:
1617 739 409 281 241 238 174 20
7
BmtI G_CTAG'C
Cuts at: 0 2921 3726
Size: 2921 805
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1311 3327 3666 3726
Size: 1311 2016 339 60
Fragments arranged by size:
2016 1311 339 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 781 969 1281 2494 2645 2764 3726
Size: 781 188 312 1213 151 119 962
Fragments arranged by size:
1213 962 781 312 188 151 119
Bpu10I CC'TnA_GC
Cuts at: 0 350 3111 3451 3726
Size: 350 2761 340 275
Fragments arranged by size:
2761 350 340 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 217 465 3125 3648 3726
Size: 217 248 2660 523 78
Fragments arranged by size:
2660 523 248 217 78
BsaI GGTCTCn'nnnn_
Cuts at: 0 745 3726
Size: 745 2981
BsaAI yAC'GTr
Cuts at: 0 3003 3726
Size: 3003 723
BsaBI GATnn'nnATC
Cuts at: 0 541 628 3726
Size: 541 87 3098
Fragments arranged by size:
3098 541 87
BsaHI Gr'CG_yC
Cuts at: 0 1953 3726
Size: 1953 1773
BsaWI w'CCGG_w
Cuts at: 0 12 1313 1338 3726
Size: 12 1301 25 2388
Fragments arranged by size:
2388 1301 25 12
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1102 1132 1702 1732 3726
Size: 1102 30 570 30 1994
Fragments arranged by size:
1994 1102 570 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1269 1390 1393 1660 1765 2356 2830 3079
Size: 1269 121 3 267 105 591 474 249
Cuts at: 3079 3093 3303 3726
Size: 14 210 423
Fragments arranged by size:
1269 591 474 423 267 249 210 121
105 14 3
BseYI C'CCAG_C
Cuts at: 0 112 433 880 1014 1045 1258 1519 1999
Size: 112 321 447 134 31 213 261 480
Cuts at: 1999 2035 2267 2321 2479 2774 2947 3177 3508
Size: 36 232 54 158 295 173 230 331
Cuts at: 3508 3726
Size: 218
Fragments arranged by size:
480 447 331 321 295 261 232 230
218 213 173 158 134 112 54 36
31
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 1078 1464 2638 2885 3287 3726
Size: 322 756 386 1174 247 402 439
Fragments arranged by size:
1174 756 439 402 386 322 247
BsiEI CG_ry'CG
Cuts at: 0 608 3726
Size: 608 3118
BsiHKAI G_wGCw'C
Cuts at: 0 275 2872 3586 3726
Size: 275 2597 714 140
Fragments arranged by size:
2597 714 275 140
BsmI GAATG_Cn'
Cuts at: 0 475 3726
Size: 475 3251
Bsp1286I G_dGCh'C
Cuts at: 0 10 121 275 464 698 1418 1504 1733
Size: 10 111 154 189 234 720 86 229
Cuts at: 1733 1793 1868 1909 2352 2480 2622 2669 2677
Size: 60 75 41 443 128 142 47 8
Cuts at: 2677 2872 2899 2948 3187 3353 3477 3574 3586
Size: 195 27 49 239 166 124 97 12
Cuts at: 3586 3726
Size: 140
Fragments arranged by size:
720 443 239 234 229 195 189 166
154 142 140 128 124 111 97 86
75 60 49 47 41 27 12 10
8
BspEI T'CCGG_A
Cuts at: 0 12 1313 1338 3726
Size: 12 1301 25 2388
Fragments arranged by size:
2388 1301 25 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 62 705 3726
Size: 62 643 3021
Fragments arranged by size:
3021 643 62
BsrBI CCG'CTC
Cuts at: 0 1104 1118 1739 3361 3662 3726
Size: 1104 14 621 1622 301 64
Fragments arranged by size:
1622 1104 621 301 64 14
BsrDI GCAATG_nn'
Cuts at: 0 1473 2296 3053 3726
Size: 1473 823 757 673
Fragments arranged by size:
1473 823 757 673
BsrFI r'CCGG_y
Cuts at: 0 854 1070 1131 3284 3726
Size: 854 216 61 2153 442
Fragments arranged by size:
2153 854 442 216 61
BssHII G'CGCG_C
Cuts at: 0 96 124 3726
Size: 96 28 3602
Fragments arranged by size:
3602 96 28
BssSI C'ACGA_G
Cuts at: 0 748 1503 2574 3726
Size: 748 755 1071 1152
Fragments arranged by size:
1152 1071 755 748
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1537 2649 3121 3726
Size: 1537 1112 472 605
Fragments arranged by size:
1537 1112 605 472
BstXI CCAn_nnnn'nTGG
Cuts at: 0 516 888 948 3336 3726
Size: 516 372 60 2388 390
Fragments arranged by size:
2388 516 390 372 60
BstYI r'GATC_y
Cuts at: 0 1545 2305 2592 3726
Size: 1545 760 287 1134
Fragments arranged by size:
1545 1134 760 287
Bsu36I CC'TnA_GG
Cuts at: 0 2258 3575 3726
Size: 2258 1317 151
Fragments arranged by size:
2258 1317 151
BtgI C'CryG_G
Cuts at: 0 608 1197 1225 1368 3089 3726
Size: 608 589 28 143 1721 637
Fragments arranged by size:
1721 637 608 589 143 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 35 267 1277 3726
Size: 35 232 1010 2449
Fragments arranged by size:
2449 1010 232 35
BtsI GCAGTG_nn'
Cuts at: 0 1335 1695 1890 2948 3726
Size: 1335 360 195 1058 778
Fragments arranged by size:
1335 1058 778 360 195
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3480 3515 3726
Size: 3480 35 211
Fragments arranged by size:
3480 211 35
DraIII CAC_nnn'GTG
Cuts at: 0 249 3325 3726
Size: 249 3076 401
Fragments arranged by size:
3076 401 249
EaeI y'GGCC_r
Cuts at: 0 101 605 642 938 1154 1194 1470 1872
Size: 101 504 37 296 216 40 276 402
Cuts at: 1872 3726
Size: 1854
Fragments arranged by size:
1854 504 402 296 276 216 101 40
37
EagI C'GGCC_G
Cuts at: 0 605 3726
Size: 605 3121
EarI CTCTTCn'nnn_
Cuts at: 0 1225 2430 2795 3726
Size: 1225 1205 365 931
Fragments arranged by size:
1225 1205 931 365
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 304 781 1710 2356 3303 3726
Size: 304 477 929 646 947 423
Fragments arranged by size:
947 929 646 477 423 304
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 781 969 1281 2494 2645 2764 3726
Size: 523 258 188 312 1213 151 119 962
Fragments arranged by size:
1213 962 523 312 258 188 151 119
EcoNI CCTnn'n_nnAGG
Cuts at: 0 427 2183 3726
Size: 427 1756 1543
Fragments arranged by size:
1756 1543 427
EcoO109I rG'GnC_Cy
Cuts at: 0 117 469 513 694 1414 1415 2262 2896
Size: 117 352 44 181 720 1 847 634
Cuts at: 2896 3183 3193 3726
Size: 287 10 533
Fragments arranged by size:
847 720 634 533 352 287 181 117
44 10 1
FspI TGC'GCA
Cuts at: 0 40 58 3726
Size: 40 18 3668
Fragments arranged by size:
3668 40 18
HaeII r_GCGC'y
Cuts at: 0 176 639 1088 2328 3212 3683 3726
Size: 176 463 449 1240 884 471 43
Fragments arranged by size:
1240 884 471 463 449 176 43
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 1926 1958 2407 2439 2970 3002
Size: 13 32 1881 32 449 32 531 32
Cuts at: 3002 3123 3155 3726
Size: 121 32 571
Fragments arranged by size:
1881 571 531 449 121 32 32 32
32 32 13
KpnI G_GTAC'C
Cuts at: 0 321 3726
Size: 321 3405
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 845 2246 2264 3726
Size: 845 1401 18 1462
Fragments arranged by size:
1462 1401 845 18
MscI TGG'CCA
Cuts at: 0 644 1196 1472 3726
Size: 644 552 276 2254
Fragments arranged by size:
2254 644 552 276
MslI CAynn'nnrTG
Cuts at: 0 344 670 1126 1657 1823 1840 1990 2123
Size: 344 326 456 531 166 17 150 133
Cuts at: 2123 2200 2827 2971 2977 3220 3279 3334 3726
Size: 77 627 144 6 243 59 55 392
Fragments arranged by size:
627 531 456 392 344 326 243 166
150 144 133 77 59 55 17 6
MspA1I CmG'CkG
Cuts at: 0 314 336 404 410 604 621 1142 1370
Size: 314 22 68 6 194 17 521 228
Cuts at: 1370 1478 2003 2162 2542 3019 3638 3726
Size: 108 525 159 380 477 619 88
Fragments arranged by size:
619 525 521 477 380 314 228 194
159 108 88 68 22 17 6
NaeI GCC'GGC
Cuts at: 0 856 1133 3286 3726
Size: 856 277 2153 440
Fragments arranged by size:
2153 856 440 277
NcoI C'CATG_G
Cuts at: 0 1197 3089 3726
Size: 1197 1892 637
Fragments arranged by size:
1892 1197 637
NgoMIV G'CCGG_C
Cuts at: 0 854 1131 3284 3726
Size: 854 277 2153 442
Fragments arranged by size:
2153 854 442 277
NheI G'CTAG_C
Cuts at: 0 2917 3726
Size: 2917 809
NspI r_CATG'y
Cuts at: 0 75 747 1839 2205 3045 3726
Size: 75 672 1092 366 840 681
Fragments arranged by size:
1092 840 681 672 366 75
PasI CC'CwG_GG
Cuts at: 0 1347 1849 2586 3080 3630 3726
Size: 1347 502 737 494 550 96
Fragments arranged by size:
1347 737 550 502 494 96
PciI A'CATG_T
Cuts at: 0 2201 3726
Size: 2201 1525
PflMI CCAn_nnn'nTGG
Cuts at: 0 1684 1968 3726
Size: 1684 284 1758
Fragments arranged by size:
1758 1684 284
PfoI T'CCnGG_A
Cuts at: 0 758 2071 3726
Size: 758 1313 1655
Fragments arranged by size:
1655 1313 758
PpuMI rG'GwC_Cy
Cuts at: 0 469 2262 3193 3726
Size: 469 1793 931 533
Fragments arranged by size:
1793 931 533 469
PspOMI G'GGCC_C
Cuts at: 0 117 694 1414 2618 2895 3183 3726
Size: 117 577 720 1204 277 288 543
Fragments arranged by size:
1204 720 577 543 288 277 117
PspXI vC'TCGA_Gb
Cuts at: 0 130 3726
Size: 130 3596
PstI C_TGCA'G
Cuts at: 0 409 620 1545 2513 3063 3726
Size: 409 211 925 968 550 663
Fragments arranged by size:
968 925 663 550 409 211
PvuII CAG'CTG
Cuts at: 0 314 336 404 621 2542 3019 3726
Size: 314 22 68 217 1921 477 707
Fragments arranged by size:
1921 707 477 314 217 68 22
SacII CC_GC'GG
Cuts at: 0 1371 3726
Size: 1371 2355
ScaI AGT'ACT
Cuts at: 0 136 3726
Size: 136 3590
SexAI A'CCwGG_T
Cuts at: 0 245 3726
Size: 245 3481
SfcI C'TryA_G
Cuts at: 0 331 405 616 1541 2509 3059 3726
Size: 331 74 211 925 968 550 667
Fragments arranged by size:
968 925 667 550 331 211 74
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 772 3726
Size: 772 2954
SmaI CCC'GGG
Cuts at: 0 265 699 2331 2678 2910 2938 3220 3334
Size: 265 434 1632 347 232 28 282 114
Cuts at: 3334 3726
Size: 392
Fragments arranged by size:
1632 434 392 347 282 265 232 114
28
SmlI C'TyrA_G
Cuts at: 0 130 196 444 3140 3663 3726
Size: 130 66 248 2696 523 63
Fragments arranged by size:
2696 523 248 130 66 63
SphI G_CATG'C
Cuts at: 0 75 3726
Size: 75 3651
SspI AAT'ATT
Cuts at: 0 2694 3726
Size: 2694 1032
StuI AGG'CCT
Cuts at: 0 2931 3456 3726
Size: 2931 525 270
Fragments arranged by size:
2931 525 270
StyI C'CwwG_G
Cuts at: 0 423 509 1197 1514 1772 2597 3089 3479
Size: 423 86 688 317 258 825 492 390
Cuts at: 3479 3726
Size: 247
Fragments arranged by size:
825 688 492 423 390 317 258 247
86
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1393 2335 2437 3336 3726
Size: 1393 942 102 899 390
Fragments arranged by size:
1393 942 899 390 102
TatI w'GTAC_w
Cuts at: 0 134 1649 2819 3726
Size: 134 1515 1170 907
Fragments arranged by size:
1515 1170 907 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1102 1134 1702 1734 3726
Size: 1102 32 568 32 1992
Fragments arranged by size:
1992 1102 568 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1617 3726
Size: 1617 2109
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1825 2148 2559 3087 3726
Size: 1825 323 411 528 639
Fragments arranged by size:
1825 639 528 411 323
XhoI C'TCGA_G
Cuts at: 0 130 3726
Size: 130 3596
XmaI C'CCGG_G
Cuts at: 0 263 697 2329 2676 2908 2936 3218 3332
Size: 263 434 1632 347 232 28 282 114
Cuts at: 3332 3726
Size: 394
Fragments arranged by size:
1632 434 394 347 282 263 232 114
28
Enzymes that do cut:
AarI Acc65I AcuI AflIII AleI AlfI AloI AlwNI
ApaI ApaLI ApoI AscI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtgZI BtsI CspCI DraIII EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NaeI NcoI NgoMIV NheI
NspI PasI PciI PflMI PfoI PpuMI PspOMI PspXI
PstI PvuII SacII ScaI SexAI SfcI SfiI SmaI
SmlI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AgeI AhdI AseI
AsiSI AvrII BaeI BbeI BfrBI BsiWI BsmBI BspHI
BsrGI BstBI BstEII BstZ17I ClaI DraI DrdI EcoICRI
EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI
KasI MfeI MluI NarI NdeI NotI NruI NsiI
PacI PmeI PmlI PpiI PshAI PsiI PsrI PvuI
RsrII SacI SalI SanDI SapI SbfI SfoI SgfI
SgrAI SnaBI SpeI SrfI SwaI XbaI XmnI ZraI