Restriction map for
pFN21ASDA2005
(Linear) (Six-base) MAPSORT of: KIAA2005.seq Check: 876 from: 1 to: 4752
KIAA2005.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 2432 3225 3377 3844 4752
Size: 2432 793 152 467 908
Fragments arranged by size:
2432 908 793 467 152
Acc65I G'GTAC_C
Cuts at: 0 2239 4752
Size: 2239 2513
AclI AA'CG_TT
Cuts at: 0 207 4752
Size: 207 4545
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1160 1550 1637 1907 2102 2268 2638 3616
Size: 1160 390 87 270 195 166 370 978
Cuts at: 3616 4359 4607 4752
Size: 743 248 145
Fragments arranged by size:
1160 978 743 390 370 270 248 195
166 145 87
AflII C'TTAA_G
Cuts at: 0 79 4752
Size: 79 4673
AflIII A'CryG_T
Cuts at: 0 1436 3569 4752
Size: 1436 2133 1183
Fragments arranged by size:
2133 1436 1183
AleI CACnn'nnGTG
Cuts at: 0 628 2950 4752
Size: 628 2322 1802
Fragments arranged by size:
2322 1802 628
ApoI r'AATT_y
Cuts at: 0 104 336 646 687 977 1104 1624 2023
Size: 104 232 310 41 290 127 520 399
Cuts at: 2023 3054 3088 3345 3774 4143 4509 4752
Size: 1031 34 257 429 369 366 243
Fragments arranged by size:
1031 520 429 399 369 366 310 290
257 243 232 127 104 41 34
AseI AT'TA_AT
Cuts at: 0 86 3172 4752
Size: 86 3086 1580
Fragments arranged by size:
3086 1580 86
AvrII C'CTAG_G
Cuts at: 0 4704 4752
Size: 4704 48
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2934 2967 4752
Size: 2934 33 1785
Fragments arranged by size:
2934 1785 33
BamHI G'GATC_C
Cuts at: 0 3930 4752
Size: 3930 822
BanI G'GyrC_C
Cuts at: 0 703 2239 2608 4752
Size: 703 1536 369 2144
Fragments arranged by size:
2144 1536 703 369
BbsI GAAGACnn'nnnn_
Cuts at: 0 80 654 1427 2888 4752
Size: 80 574 773 1461 1864
Fragments arranged by size:
1864 1461 773 574 80
BciVI GTATCCnnnnn_n'
Cuts at: 0 2088 4752
Size: 2088 2664
BclI T'GATC_A
Cuts at: 0 504 800 1352 2342 2669 4752
Size: 504 296 552 990 327 2083
Fragments arranged by size:
2083 990 552 504 327 296
BfrBI ATG'CAT
Cuts at: 0 2256 3244 4752
Size: 2256 988 1508
Fragments arranged by size:
2256 1508 988
BglII A'GATC_T
Cuts at: 0 1773 3786 4752
Size: 1773 2013 966
Fragments arranged by size:
2013 1773 966
Bme1580I G_kGCm'C
Cuts at: 0 3318 4752
Size: 3318 1434
BmgBI CAC'GTC
Cuts at: 0 3319 4752
Size: 3319 1433
BmrI ACTGGGnnnn_n'
Cuts at: 0 3321 4752
Size: 3321 1431
BmtI G_CTAG'C
Cuts at: 0 1043 4752
Size: 1043 3709
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1693 4752
Size: 1693 3059
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2050 3952 4752
Size: 2050 1902 800
Fragments arranged by size:
2050 1902 800
Bpu10I CC'TnA_GC
Cuts at: 0 3261 4752
Size: 3261 1491
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3231 3462 4752
Size: 3231 231 1290
Fragments arranged by size:
3231 1290 231
BsaBI GATnn'nnATC
Cuts at: 0 362 672 4752
Size: 362 310 4080
Fragments arranged by size:
4080 362 310
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 731 761 847 877 3699 3729 4752
Size: 731 30 86 30 2822 30 1023
Fragments arranged by size:
2822 1023 731 86 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3988 4752
Size: 3988 764
BseYI C'CCAG_C
Cuts at: 0 1477 2171 4752
Size: 1477 694 2581
Fragments arranged by size:
2581 1477 694
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1946 3236 4419 4752
Size: 1946 1290 1183 333
Fragments arranged by size:
1946 1290 1183 333
BsiHKAI G_wGCw'C
Cuts at: 0 567 4752
Size: 567 4185
BsmI GAATG_Cn'
Cuts at: 0 3244 4752
Size: 3244 1508
BsmBI CGTCTCn'nnnn_
Cuts at: 0 3552 4752
Size: 3552 1200
Bsp1286I G_dGCh'C
Cuts at: 0 567 3318 4752
Size: 567 2751 1434
Fragments arranged by size:
2751 1434 567
BspHI T'CATG_A
Cuts at: 0 252 2666 2672 4752
Size: 252 2414 6 2080
Fragments arranged by size:
2414 2080 252 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 4391 4752
Size: 4391 361
BsrDI GCAATG_nn'
Cuts at: 0 660 1169 4752
Size: 660 509 3583
Fragments arranged by size:
3583 660 509
BsrGI T'GTAC_A
Cuts at: 0 1990 4752
Size: 1990 2762
BstAPI GCAn_nnn'nTGC
Cuts at: 0 108 2329 4752
Size: 108 2221 2423
Fragments arranged by size:
2423 2221 108
BstEII G'GTnAC_C
Cuts at: 0 2352 3539 4752
Size: 2352 1187 1213
Fragments arranged by size:
2352 1213 1187
BstXI CCAn_nnnn'nTGG
Cuts at: 0 630 1547 4752
Size: 630 917 3205
Fragments arranged by size:
3205 917 630
BstYI r'GATC_y
Cuts at: 0 1671 1773 1915 2521 3786 3930 4369 4752
Size: 1671 102 142 606 1265 144 439 383
Fragments arranged by size:
1671 1265 606 439 383 144 142 102
BstZ17I GTA'TAC
Cuts at: 0 3845 4752
Size: 3845 907
BtgI C'CryG_G
Cuts at: 0 181 623 735 1911 4067 4752
Size: 181 442 112 1176 2156 685
Fragments arranged by size:
2156 1176 685 442 181 112
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 508 4752
Size: 508 4244
BtsI GCAGTG_nn'
Cuts at: 0 2166 4752
Size: 2166 2586
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 848 883 4752
Size: 848 35 3869
Fragments arranged by size:
3869 848 35
DraI TTT'AAA
Cuts at: 0 1109 1304 1835 4752
Size: 1109 195 531 2917
Fragments arranged by size:
2917 1109 531 195
DraIII CAC_nnn'GTG
Cuts at: 0 2824 4752
Size: 2824 1928
DrdI GACnn_nn'nnGTC
Cuts at: 0 907 4752
Size: 907 3845
EaeI y'GGCC_r
Cuts at: 0 1058 4154 4303 4752
Size: 1058 3096 149 449
Fragments arranged by size:
3096 1058 449 149
EarI CTCTTCn'nnn_
Cuts at: 0 1139 1656 3044 3075 3116 4752
Size: 1139 517 1388 31 41 1636
Fragments arranged by size:
1636 1388 1139 517 41 31
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1160 1550 1637 1907 2050 2102 2268 2638
Size: 1160 390 87 270 143 52 166 370
Cuts at: 2638 3616 3952 4359 4607 4752
Size: 978 336 407 248 145
Fragments arranged by size:
1160 978 407 390 370 336 270 248
166 145 143 87 52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 557 4752
Size: 557 4195
EcoO109I rG'GnC_Cy
Cuts at: 0 159 4273 4723 4752
Size: 159 4114 450 29
Fragments arranged by size:
4114 450 159 29
EcoRI G'AATT_C
Cuts at: 0 687 4752
Size: 687 4065
EcoRV GAT'ATC
Cuts at: 0 384 1053 4752
Size: 384 669 3699
Fragments arranged by size:
3699 669 384
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 792 2298 3628 4712 4752
Size: 792 1506 1330 1084 40
Fragments arranged by size:
1506 1330 1084 792 40
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1330 1362 1440 1472 3523 3555 3699 3731
Size: 1330 32 78 32 2051 32 144 32
Cuts at: 3731 4752
Size: 1021
Fragments arranged by size:
2051 1330 1021 144 78 32 32 32
32
HincII GTy'rAC
Cuts at: 0 2742 4752
Size: 2742 2010
HindIII A'AGCT_T
Cuts at: 0 1370 2149 2874 3179 4752
Size: 1370 779 725 305 1573
Fragments arranged by size:
1573 1370 779 725 305
KpnI G_GTAC'C
Cuts at: 0 2243 4752
Size: 2243 2509
MfeI C'AATT_G
Cuts at: 0 478 4752
Size: 478 4274
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1310 3383 4752
Size: 1310 2073 1369
Fragments arranged by size:
2073 1369 1310
MscI TGG'CCA
Cuts at: 0 1060 4156 4305 4752
Size: 1060 3096 149 447
Fragments arranged by size:
3096 1060 447 149
MslI CAynn'nnrTG
Cuts at: 0 628 640 791 970 1545 2389 2671 2950
Size: 628 12 151 179 575 844 282 279
Cuts at: 2950 4752
Size: 1802
Fragments arranged by size:
1802 844 628 575 282 279 179 151
12
MspA1I CmG'CkG
Cuts at: 0 1481 4752
Size: 1481 3271
NcoI C'CATG_G
Cuts at: 0 181 735 4067 4752
Size: 181 554 3332 685
Fragments arranged by size:
3332 685 554 181
NdeI CA'TA_TG
Cuts at: 0 3680 4734 4752
Size: 3680 1054 18
Fragments arranged by size:
3680 1054 18
NheI G'CTAG_C
Cuts at: 0 1039 4752
Size: 1039 3713
NsiI A_TGCA'T
Cuts at: 0 2258 3246 4752
Size: 2258 988 1506
Fragments arranged by size:
2258 1506 988
NspI r_CATG'y
Cuts at: 0 55 1440 2260 3573 4398 4752
Size: 55 1385 820 1313 825 354
Fragments arranged by size:
1385 1313 825 820 354 55
PasI CC'CwG_GG
Cuts at: 0 2861 4752
Size: 2861 1891
PciI A'CATG_T
Cuts at: 0 1436 3569 4752
Size: 1436 2133 1183
Fragments arranged by size:
2133 1436 1183
PflMI CCAn_nnn'nTGG
Cuts at: 0 2233 4752
Size: 2233 2519
PfoI T'CCnGG_A
Cuts at: 0 258 2027 2524 3968 4752
Size: 258 1769 497 1444 784
Fragments arranged by size:
1769 1444 784 497 258
PpuMI rG'GwC_Cy
Cuts at: 0 159 4273 4752
Size: 159 4114 479
Fragments arranged by size:
4114 479 159
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2886 2918 4752
Size: 2886 32 1834
Fragments arranged by size:
2886 1834 32
PstI C_TGCA'G
Cuts at: 0 143 884 4106 4752
Size: 143 741 3222 646
Fragments arranged by size:
3222 741 646 143
PvuII CAG'CTG
Cuts at: 0 1481 4752
Size: 1481 3271
SbfI CC_TGCA'GG
Cuts at: 0 143 4752
Size: 143 4609
ScaI AGT'ACT
Cuts at: 0 4486 4752
Size: 4486 266
SfcI C'TryA_G
Cuts at: 0 139 880 2358 3420 4102 4604 4752
Size: 139 741 1478 1062 682 502 148
Fragments arranged by size:
1478 1062 741 682 502 148 139
SmlI C'TyrA_G
Cuts at: 0 79 3210 3477 4752
Size: 79 3131 267 1275
Fragments arranged by size:
3131 1275 267 79
SpeI A'CTAG_T
Cuts at: 0 2851 4752
Size: 2851 1901
SspI AAT'ATT
Cuts at: 0 354 2195 2831 4752
Size: 354 1841 636 1921
Fragments arranged by size:
1921 1841 636 354
StyI C'CwwG_G
Cuts at: 0 181 735 859 2588 4067 4704 4752
Size: 181 554 124 1729 1479 637 48
Fragments arranged by size:
1729 1479 637 554 181 124 48
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1874 3583 4752
Size: 1874 1709 1169
Fragments arranged by size:
1874 1709 1169
TatI w'GTAC_w
Cuts at: 0 1990 2854 2981 4385 4484 4752
Size: 1990 864 127 1404 99 268
Fragments arranged by size:
1990 1404 864 268 127 99
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1810 1842 1955 1987 4752
Size: 1810 32 113 32 2765
Fragments arranged by size:
2765 1810 113 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1884 4256 4752
Size: 1884 2372 496
Fragments arranged by size:
2372 1884 496
XbaI T'CTAG_A
Cuts at: 0 1126 1533 4752
Size: 1126 407 3219
Fragments arranged by size:
3219 1126 407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3678 4752
Size: 3678 1074
XmnI GAAnn'nnTTC
Cuts at: 0 401 875 2281 2898 3058 4752
Size: 401 474 1406 617 160 1694
Fragments arranged by size:
1694 1406 617 474 401 160
Enzymes that do cut:
AccI Acc65I AclI AcuI AflII AflIII AleI ApoI
AseI AvrII BaeI BamHI BanI BbsI BciVI BclI
BfrBI BglII Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaBI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspHI BspMI BsrDI BsrGI BstAPI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI CspCI
DraI DraIII DrdI EaeI EarI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI Hin4I HincII HindIII KpnI MfeI
MmeI MscI MslI MspA1I NcoI NdeI NheI NsiI
NspI PasI PciI PflMI PfoI PpuMI PsrI PstI
PvuII SbfI ScaI SfcI SmlI SpeI SspI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AfeI AgeI AhdI AlfI AloI AlwNI
ApaI ApaLI AscI AsiSI AvaI BanII BbeI BbvCI
BcgI BglI BlpI BsaI BsaAI BsaHI BsaWI BsiEI
BsiWI BspEI BsrBI BsrFI BssHII BssSI BstBI Bsu36I
ClaI EagI EciI EcoICRI FseI FspI FspAI HaeII
HpaI KasI MluI NaeI NarI NgoMIV NotI NruI
PacI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI
PvuI RsrII SacI SacII SalI SanDI SapI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SphI SrfI
StuI SwaI XhoI XmaI ZraI