Restriction map for
pFN21ASDA2003
(Linear) (Six-base) MAPSORT of: KIAA2003.seq Check: 1821 from: 1 to: 1311
KIAA2003.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 277 1311
Size: 277 1034
ApaLI G'TGCA_C
Cuts at: 0 1242 1311
Size: 1242 69
ApoI r'AATT_y
Cuts at: 0 1188 1298 1311
Size: 1188 110 13
Fragments arranged by size:
1188 110 13
BamHI G'GATC_C
Cuts at: 0 889 1311
Size: 889 422
BbsI GAAGACnn'nnnn_
Cuts at: 0 238 1311
Size: 238 1073
Bme1580I G_kGCm'C
Cuts at: 0 37 1246 1311
Size: 37 1209 65
Fragments arranged by size:
1209 65 37
BmrI ACTGGGnnnn_n'
Cuts at: 0 344 728 812 1064 1232 1311
Size: 344 384 84 252 168 79
Fragments arranged by size:
384 344 252 168 84 79
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 572 992 1092 1260 1311
Size: 151 421 420 100 168 51
Fragments arranged by size:
421 420 168 151 100 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 91 1311
Size: 91 1220
BsaBI GATnn'nnATC
Cuts at: 0 164 1311
Size: 164 1147
BsaHI Gr'CG_yC
Cuts at: 0 22 1311
Size: 22 1289
BsaWI w'CCGG_w
Cuts at: 0 620 1311
Size: 620 691
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 758 759 843 927 1010 1011 1095
Size: 222 536 1 84 84 83 1 84
Cuts at: 1095 1179 1311
Size: 84 132
Fragments arranged by size:
536 222 132 84 84 84 84 83
1 1
BsiHKAI G_wGCw'C
Cuts at: 0 1246 1311
Size: 1246 65
BsmI GAATG_Cn'
Cuts at: 0 421 1311
Size: 421 890
Bsp1286I G_dGCh'C
Cuts at: 0 37 1246 1311
Size: 37 1209 65
Fragments arranged by size:
1209 65 37
BspHI T'CATG_A
Cuts at: 0 648 1311
Size: 648 663
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 244 277 1311
Size: 244 33 1034
Fragments arranged by size:
1034 244 33
BsrDI GCAATG_nn'
Cuts at: 0 213 1311
Size: 213 1098
BstYI r'GATC_y
Cuts at: 0 889 1311
Size: 889 422
BtsI GCAGTG_nn'
Cuts at: 0 495 632 747 831 1083 1167 1311
Size: 495 137 115 84 252 84 144
Fragments arranged by size:
495 252 144 137 115 84 84
EaeI y'GGCC_r
Cuts at: 0 8 56 296 1311
Size: 8 48 240 1015
Fragments arranged by size:
1015 240 48 8
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 572 992 1092 1260 1311
Size: 151 421 420 100 168 51
Fragments arranged by size:
421 420 168 151 100 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 1311
Size: 94 1217
EcoRI G'AATT_C
Cuts at: 0 1298 1311
Size: 1298 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 12 44 1311
Size: 12 32 1267
Fragments arranged by size:
1267 32 12
MscI TGG'CCA
Cuts at: 0 298 1311
Size: 298 1013
MslI CAynn'nnrTG
Cuts at: 0 653 735 1311
Size: 653 82 576
Fragments arranged by size:
653 576 82
MspA1I CmG'CkG
Cuts at: 0 7 428 1311
Size: 7 421 883
Fragments arranged by size:
883 421 7
NdeI CA'TA_TG
Cuts at: 0 484 732 1311
Size: 484 248 579
Fragments arranged by size:
579 484 248
PsiI TTA'TAA
Cuts at: 0 986 1311
Size: 986 325
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 450 482 1311
Size: 450 32 829
Fragments arranged by size:
829 450 32
PstI C_TGCA'G
Cuts at: 0 274 639 1311
Size: 274 365 672
Fragments arranged by size:
672 365 274
SfcI C'TryA_G
Cuts at: 0 270 467 519 635 1101 1311
Size: 270 197 52 116 466 210
Fragments arranged by size:
466 270 210 197 116 52
StuI AGG'CCT
Cuts at: 0 813 897 981 1065 1311
Size: 813 84 84 84 246
Fragments arranged by size:
813 246 84 84 84
StyI C'CwwG_G
Cuts at: 0 183 1311
Size: 183 1128
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 874 906 1311
Size: 874 32 405
Fragments arranged by size:
874 405 32
XbaI T'CTAG_A
Cuts at: 0 156 1311
Size: 156 1155
XmnI GAAnn'nnTTC
Cuts at: 0 289 1311
Size: 289 1022
Enzymes that do cut:
AarI ApaLI ApoI BamHI BbsI Bme1580I BmrI BpmI
BsaI BsaBI BsaHI BsaWI BsgI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrDI BstYI BtsI EaeI Eco57MI EcoNI
EcoRI Hin4I MscI MslI MspA1I NdeI PsiI PsrI
PstI SfcI StuI StyI TstI XbaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI AscI
AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaXI BseRI
BseYI BsiEI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoO109I EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
Tth111I XcmI XhoI XmaI ZraI