(Circular) (Six-base) MAPSORT of: pFN21ASDA1970.seq Check: 5933 from: 1 to: 6298 pFN21ASDA1970 6298 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2533 2590 2802 2850 2533 Size: 57 212 48 5981 Fragments arranged by size: 5981 212 57 48 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4108 278 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3213 3282 3641 1937 Size: 1276 69 359 4594 Fragments arranged by size: 4594 1276 359 69 Acc65I G'GTAC_C Cuts at: 3619 3619 Size: 6298 AclI AA'CG_TT Cuts at: 1563 4425 4798 1563 Size: 2862 373 3063 Fragments arranged by size: 3063 2862 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2438 2492 3449 4361 5745 6013 1632 Size: 806 54 957 912 1384 268 1917 Fragments arranged by size: 1917 1384 957 912 806 268 54 AfeI AGC'GCT Cuts at: 2412 2412 Size: 6298 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6101 AflIII A'CryG_T Cuts at: 1203 5197 6174 1203 Size: 3994 977 1327 Fragments arranged by size: 3994 1327 977 AgeI A'CCGG_T Cuts at: 5129 5129 Size: 6298 AhdI GACnn_n'nnGTC Cuts at: 3271 5027 3271 Size: 1756 4542 AleI CACnn'nnGTG Cuts at: 1166 2175 1166 Size: 1009 5289 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2931 3017 1184 Size: 1747 86 4465 Fragments arranged by size: 4465 1747 86 AlwNI CAG_nnn'CTG Cuts at: 2461 2557 2623 2812 3106 4041 5613 2461 Size: 96 66 189 294 935 1572 3146 Fragments arranged by size: 3146 1572 935 294 189 96 66 ApaI G_GGCC'C Cuts at: 2364 2364 Size: 6298 ApaLI G'TGCA_C Cuts at: 4355 5511 4355 Size: 1156 5142 ApoI r'AATT_y Cuts at: 1493 3080 3607 3833 3985 6094 1493 Size: 1587 527 226 152 2109 1697 Fragments arranged by size: 2109 1697 1587 527 226 152 AseI AT'TA_AT Cuts at: 160 1032 4852 160 Size: 872 3820 1606 Fragments arranged by size: 3820 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 AvaI C'yCGr_G Cuts at: 1870 1943 3623 1870 Size: 73 1680 4545 Fragments arranged by size: 4545 1680 73 BamHI G'GATC_C Cuts at: 3225 3570 3628 3995 3225 Size: 345 58 367 5528 Fragments arranged by size: 5528 367 345 58 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2605 3129 3455 3619 5074 Size: 325 217 627 818 524 326 164 1455 Cuts at: 5074 618 Size: 1842 Fragments arranged by size: 1842 1455 818 627 524 326 325 217 164 BanII G_rGCy'C Cuts at: 1391 2339 2364 2469 3617 1391 Size: 948 25 105 1148 4072 Fragments arranged by size: 4072 1148 948 105 25 BbeI G_GCGC'C Cuts at: 3133 3133 Size: 6298 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2426 928 Size: 971 527 4800 Fragments arranged by size: 4800 971 527 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4489 4523 1903 Size: 34 2552 34 3678 Fragments arranged by size: 3678 2552 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4190 5400 4190 Size: 1210 5088 BclI T'GATC_A Cuts at: 1548 3012 1548 Size: 1464 4834 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2160 4909 136 Size: 107 122 71 631 605 488 2749 1525 Fragments arranged by size: 2749 1525 631 605 488 122 107 71 BglII A'GATC_T Cuts at: 3386 6293 3386 Size: 2907 3391 BlpI GC'TnA_GC Cuts at: 3715 3715 Size: 6298 Bme1580I G_kGCm'C Cuts at: 1790 2114 2364 4359 5515 1790 Size: 324 250 1995 1156 2573 Fragments arranged by size: 2573 1995 1156 324 250 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6298 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2666 3203 3272 Size: 416 53 83 399 24 1249 537 69 Cuts at: 3272 4029 4987 442 Size: 757 958 1753 Fragments arranged by size: 1753 1249 958 757 537 416 399 83 69 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6298 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3420 3420 Size: 6298 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2483 2615 2924 3083 3086 4958 1239 Size: 1088 156 132 309 159 3 1872 2579 Fragments arranged by size: 2579 1872 1088 309 159 156 132 3 Bpu10I CC'TnA_GC Cuts at: 2968 2968 Size: 6298 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4423 5288 5586 4423 Size: 865 298 5135 Fragments arranged by size: 5135 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2661 4961 882 Size: 617 1162 2300 2219 Fragments arranged by size: 2300 2219 1162 617 BsaAI yAC'GTr Cuts at: 493 1204 2752 3217 3595 4089 493 Size: 711 1548 465 378 494 2702 Fragments arranged by size: 2702 1548 711 494 465 378 BsaBI GATnn'nnATC Cuts at: 6292 6292 Size: 6298 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2501 3130 3330 4105 Size: 53 83 186 936 968 629 200 775 Cuts at: 4105 4487 5122 275 Size: 382 635 1451 Fragments arranged by size: 1451 968 936 775 635 629 382 200 186 83 53 BsaWI w'CCGG_w Cuts at: 2423 4731 5129 5403 5550 5961 2423 Size: 2308 398 274 147 411 2760 Fragments arranged by size: 2760 2308 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2658 2688 3081 3111 1178 Size: 30 165 30 1255 30 393 30 4365 Fragments arranged by size: 4365 1255 393 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2053 2789 2843 3179 1186 Size: 867 736 54 336 4305 Fragments arranged by size: 4305 867 736 336 54 BseYI C'CCAG_C Cuts at: 2150 2357 2454 3370 5501 5997 2150 Size: 207 97 916 2131 496 2451 Fragments arranged by size: 2451 2131 916 496 207 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2542 3077 1163 Size: 573 806 535 4384 Fragments arranged by size: 4384 806 573 535 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3748 4509 5537 1540 Size: 275 186 1747 761 1028 2301 Fragments arranged by size: 2301 1747 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2469 2910 3322 4359 4444 5515 2469 Size: 441 412 1037 85 1071 3252 Fragments arranged by size: 3252 1071 1037 441 412 85 BsmI GAATG_Cn' Cuts at: 3809 3902 3809 Size: 93 6205 BsmBI CGTCTCn'nnnn_ Cuts at: 2782 2782 Size: 6298 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2114 2339 2364 2469 2910 3322 3617 Size: 399 324 225 25 105 441 412 295 Cuts at: 3617 4359 4444 5515 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 441 412 399 324 295 225 105 85 25 BspEI T'CCGG_A Cuts at: 5961 5961 Size: 6298 BspHI T'CATG_A Cuts at: 2679 4187 5917 2679 Size: 1508 1730 3060 Fragments arranged by size: 3060 1730 1508 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2533 2590 2802 2850 3142 3653 844 Size: 1689 57 212 48 292 511 3489 Fragments arranged by size: 3489 1689 511 292 212 57 48 BsrBI CCG'CTC Cuts at: 1394 1823 4185 1394 Size: 429 2362 3507 Fragments arranged by size: 3507 2362 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3121 4793 4967 65 Size: 1183 1873 1672 174 1396 Fragments arranged by size: 1873 1672 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4942 5129 1700 Size: 110 3132 187 2869 Fragments arranged by size: 3132 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2196 96 Size: 2100 4198 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6298 BssSI C'ACGA_G Cuts at: 4358 5370 4358 Size: 1012 5286 BstAPI GCAn_nnn'nTGC Cuts at: 794 2605 794 Size: 1811 4487 BstBI TT'CG_AA Cuts at: 2007 5102 2007 Size: 3095 3203 BstEII G'GTnAC_C Cuts at: 1184 3037 1184 Size: 1853 4445 BstYI r'GATC_y Cuts at: 1972 3225 3386 3570 3628 3995 4380 4397 5849 Size: 1253 161 184 58 367 385 17 1452 Cuts at: 5849 5935 5947 6293 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1253 385 367 346 184 161 86 58 17 12 BstZ17I GTA'TAC Cuts at: 3283 3283 Size: 6298 Bsu36I CC'TnA_GG Cuts at: 2036 2240 2036 Size: 204 6094 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4789 Fragments arranged by size: 4789 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5559 Fragments arranged by size: 5559 683 56 BtsI GCAGTG_nn' Cuts at: 2187 2781 3814 4608 4628 2187 Size: 594 1033 794 20 3857 Fragments arranged by size: 3857 1033 794 594 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6298 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6263 DraI TTT'AAA Cuts at: 3602 3602 Size: 6298 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5305 809 Size: 507 3989 1802 Fragments arranged by size: 3989 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2077 2559 2652 Size: 54 1425 50 275 18 247 482 93 Cuts at: 2652 3745 4634 6263 8 Size: 1093 889 1629 43 Fragments arranged by size: 1629 1425 1093 889 482 275 247 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3745 1537 Size: 275 1933 4090 Fragments arranged by size: 4090 1933 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2678 4228 1353 Size: 1325 1550 3423 Fragments arranged by size: 3423 1550 1325 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2579 4885 5259 5405 387 Size: 1064 138 990 2306 374 146 1280 Fragments arranged by size: 2306 1280 1064 990 374 146 138 EcoICRI GAG'CTC Cuts at: 2467 2467 Size: 6298 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2438 2483 2492 2615 2924 3083 Size: 393 695 111 45 9 123 309 159 Cuts at: 3083 3086 3449 4361 4958 5745 6013 1239 Size: 3 363 912 597 787 268 1524 Fragments arranged by size: 1524 912 787 695 597 393 363 309 268 159 123 111 45 9 3 EcoNI CCTnn'n_nnAGG Cuts at: 2474 2597 2804 3032 3161 3309 2474 Size: 123 207 228 129 148 5463 Fragments arranged by size: 5463 228 207 148 129 123 EcoO109I rG'GnC_Cy Cuts at: 2361 2566 2620 2809 2997 3154 3515 2361 Size: 205 54 189 188 157 361 5144 Fragments arranged by size: 5144 361 205 189 188 157 54 EcoRI G'AATT_C Cuts at: 3080 3607 3985 3080 Size: 527 378 5393 Fragments arranged by size: 5393 527 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2500 2500 Size: 6298 FspI TGC'GCA Cuts at: 4804 4804 Size: 6298 HaeII r_GCGC'y Cuts at: 1678 2033 2414 3133 5445 1678 Size: 355 381 719 2312 2531 Fragments arranged by size: 2531 2312 719 381 355 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2658 2690 3081 3113 3229 Size: 32 135 32 1086 32 391 32 116 Cuts at: 3229 3257 3261 3289 3320 3352 4939 4971 5013 Size: 28 4 28 31 32 1587 32 42 Cuts at: 5013 5045 1373 Size: 32 2626 Fragments arranged by size: 2626 1587 1086 391 135 116 42 32 32 32 32 32 32 32 31 28 28 4 HincII GTy'rAC Cuts at: 669 1657 1938 3642 3888 669 Size: 988 281 1704 246 3079 Fragments arranged by size: 3079 1704 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6298 HpaI GTT'AAC Cuts at: 3888 3888 Size: 6298 KasI G'GCGC_C Cuts at: 3129 3129 Size: 6298 KpnI G_GTAC'C Cuts at: 3623 3623 Size: 6298 MfeI C'AATT_G Cuts at: 3897 3897 Size: 6298 MluI A'CGCG_T Cuts at: 6174 6174 Size: 6298 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2364 2925 5412 5596 1305 Size: 1059 561 2487 184 2007 Fragments arranged by size: 2487 2007 1059 561 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2561 2654 10 Size: 54 1425 343 729 93 3654 Fragments arranged by size: 3654 1425 729 343 93 54 MslI CAynn'nnrTG Cuts at: 518 1166 2175 4256 4615 4774 518 Size: 648 1009 2081 359 159 2042 Fragments arranged by size: 2081 2042 1009 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2285 2380 2458 3103 3136 3184 3325 3714 Size: 679 95 78 645 33 48 141 389 Cuts at: 3714 4391 5539 5784 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 679 677 645 389 245 141 95 78 48 33 NarI GG'CG_CC Cuts at: 3130 3130 Size: 6298 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4789 Fragments arranged by size: 4789 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6298 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6298 NotI GC'GGCC_GC Cuts at: 3745 3745 Size: 6298 NspI r_CATG'y Cuts at: 1132 1229 2305 3656 5201 1132 Size: 97 1076 1351 1545 2229 Fragments arranged by size: 2229 1545 1351 1076 97 PasI CC'CwG_GG Cuts at: 1348 2812 1348 Size: 1464 4834 PciI A'CATG_T Cuts at: 5197 5197 Size: 6298 PflMI CCAn_nnn'nTGG Cuts at: 1108 2461 2557 2623 2737 2812 3106 3139 3580 Size: 1353 96 66 114 75 294 33 441 Cuts at: 3580 1108 Size: 3826 Fragments arranged by size: 3826 1353 441 294 114 96 75 66 33 PfoI T'CCnGG_A Cuts at: 2616 2901 2616 Size: 285 6013 PmeI GTTT'AAAC Cuts at: 3602 3602 Size: 6298 PmlI CAC'GTG Cuts at: 2752 2752 Size: 6298 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4367 4399 5880 5912 4367 Size: 32 1481 32 4753 Fragments arranged by size: 4753 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2566 2620 2997 3154 3515 2566 Size: 54 377 157 361 5349 Fragments arranged by size: 5349 377 361 157 54 PshAI GACnn'nnGTC Cuts at: 1613 3035 1613 Size: 1422 4876 PsiI TTA'TAA Cuts at: 3868 3868 Size: 6298 PspOMI G'GGCC_C Cuts at: 2360 2360 Size: 6298 PstI C_TGCA'G Cuts at: 830 2052 3153 3264 3310 3650 830 Size: 1222 1101 111 46 340 3478 Fragments arranged by size: 3478 1222 1101 340 111 46 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6298 PvuII CAG'CTG Cuts at: 3136 3184 3325 3136 Size: 48 141 6109 Fragments arranged by size: 6109 141 48 SacI G_AGCT'C Cuts at: 2469 2469 Size: 6298 SalI G'TCGA_C Cuts at: 1936 3640 1936 Size: 1704 4594 SapI GCTCTTCn'nnn_ Cuts at: 2678 2678 Size: 6298 SbfI CC_TGCA'GG Cuts at: 3264 3650 3264 Size: 386 5912 ScaI AGT'ACT Cuts at: 4546 6258 4546 Size: 1712 4586 SexAI A'CCwGG_T Cuts at: 2622 2622 Size: 6298 SfcI C'TryA_G Cuts at: 826 1045 2048 3149 3260 3306 3522 3646 4781 Size: 219 1003 1101 111 46 216 124 1135 Cuts at: 4781 5462 5653 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1101 1003 681 219 216 191 124 111 46 SfoI GGC'GCC Cuts at: 3131 3131 Size: 6298 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6298 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6298 SmaI CCC'GGG Cuts at: 1872 3625 1872 Size: 1753 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4402 5303 5565 820 Size: 197 926 2459 901 262 1553 Fragments arranged by size: 2459 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3595 4089 493 Size: 3102 494 2702 Fragments arranged by size: 3102 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6298 SphI G_CATG'C Cuts at: 1132 3656 1132 Size: 2524 3774 SspI AAT'ATT Cuts at: 5 52 2293 4222 5 Size: 47 2241 1929 2081 Fragments arranged by size: 2241 2081 1929 47 StuI AGG'CCT Cuts at: 1517 2062 2483 3170 1517 Size: 545 421 687 4645 Fragments arranged by size: 4645 687 545 421 StyI C'CwwG_G Cuts at: 513 1065 2022 2562 3543 3737 513 Size: 552 957 540 981 194 3074 Fragments arranged by size: 3074 981 957 552 540 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2165 4325 4342 4495 4680 5125 325 Size: 1255 585 2160 17 153 185 445 1498 Fragments arranged by size: 2160 1498 1255 585 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2196 3462 4544 Size: 274 80 33 51 1443 219 1266 1082 Cuts at: 4544 6256 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1266 1082 274 219 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6258 6290 6258 Size: 32 6266 Tth111I GACn'n_nGTC Cuts at: 1316 2624 2672 3243 1316 Size: 1308 48 571 4371 Fragments arranged by size: 4371 1308 571 48 XbaI T'CTAG_A Cuts at: 3379 3634 3379 Size: 255 6043 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2707 3367 2707 Size: 660 5638 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6298 XmaI C'CCGG_G Cuts at: 1870 3623 1870 Size: 1753 4545 XmnI GAAnn'nnTTC Cuts at: 4427 4427 Size: 6298 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4106 276 Size: 53 83 186 936 2572 2468 Fragments arranged by size: 2572 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbvCI BfrBI BsiWI BstXI DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI