(Circular) (Six-base) MAPSORT of: pFN21ASDA1969.seq Check: 9555 from: 1 to: 6457 pFN21ASDA1969 6457 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4267 278 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3800 1937 Size: 1863 4594 Acc65I G'GTAC_C Cuts at: 3778 3778 Size: 6457 AclI AA'CG_TT Cuts at: 1563 4584 4957 1563 Size: 3021 373 3063 Fragments arranged by size: 3063 3021 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4520 5904 6172 1632 Size: 2888 1384 268 1917 Fragments arranged by size: 2888 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6260 AflIII A'CryG_T Cuts at: 1203 5356 6333 1203 Size: 4153 977 1327 Fragments arranged by size: 4153 1327 977 AgeI A'CCGG_T Cuts at: 2916 3336 5288 2916 Size: 420 1952 4085 Fragments arranged by size: 4085 1952 420 AhdI GACnn_n'nnGTC Cuts at: 5186 5186 Size: 6457 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6457 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2086 1184 Size: 902 5555 AlwNI CAG_nnn'CTG Cuts at: 4200 5772 4200 Size: 1572 4885 ApaLI G'TGCA_C Cuts at: 2489 4514 5670 2489 Size: 2025 1156 3276 Fragments arranged by size: 3276 2025 1156 ApoI r'AATT_y Cuts at: 1493 2150 2194 2443 2582 2694 2710 2778 3198 Size: 657 44 249 139 112 16 68 420 Cuts at: 3198 3450 3618 3766 3992 4144 6253 1493 Size: 252 168 148 226 152 2109 1697 Fragments arranged by size: 2109 1697 657 420 252 249 226 168 152 148 139 112 68 44 16 AseI AT'TA_AT Cuts at: 160 1032 5011 160 Size: 872 3979 1606 Fragments arranged by size: 3979 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 AvaI C'yCGr_G Cuts at: 1870 1943 3782 1870 Size: 73 1839 4545 Fragments arranged by size: 4545 1839 73 BamHI G'GATC_C Cuts at: 3787 4154 3787 Size: 367 6090 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3778 5233 618 Size: 325 217 627 1991 1455 1842 Fragments arranged by size: 1991 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2301 3776 1391 Size: 910 1475 4072 Fragments arranged by size: 4072 1475 910 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2230 2997 928 Size: 971 331 767 4388 Fragments arranged by size: 4388 971 767 331 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4648 4682 1903 Size: 34 2711 34 3678 Fragments arranged by size: 3678 2711 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2058 4349 5559 2058 Size: 2291 1210 2956 Fragments arranged by size: 2956 2291 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6457 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5068 136 Size: 107 122 71 631 605 3396 1525 Fragments arranged by size: 3396 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6452 6452 Size: 6457 BlpI GC'TnA_GC Cuts at: 2187 3874 2187 Size: 1687 4770 Bme1580I G_kGCm'C Cuts at: 1790 2493 4518 5674 1790 Size: 703 2025 1156 2573 Fragments arranged by size: 2573 2025 1156 703 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6457 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2264 2880 4188 Size: 416 53 83 399 24 847 616 1308 Cuts at: 4188 5146 442 Size: 958 1753 Fragments arranged by size: 1753 1308 958 847 616 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6457 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3735 3735 Size: 6457 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2180 2303 2808 2976 3060 3144 3228 3312 Size: 941 123 505 168 84 84 84 84 Cuts at: 3312 3396 3480 3730 5117 1239 Size: 84 84 250 1387 2579 Fragments arranged by size: 2579 1387 941 505 250 168 123 84 84 84 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2039 4582 5447 5745 2039 Size: 2543 865 298 2751 Fragments arranged by size: 2751 2543 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5120 882 Size: 617 3621 2219 Fragments arranged by size: 3621 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3754 4248 493 Size: 711 2550 494 2702 Fragments arranged by size: 2702 2550 711 494 BsaBI GATnn'nnATC Cuts at: 6451 6451 Size: 6457 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4264 4646 5281 275 Size: 53 83 186 936 2731 382 635 1451 Fragments arranged by size: 2731 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2916 3336 4890 5288 5562 5709 6120 2916 Size: 420 1554 398 274 147 411 3253 Fragments arranged by size: 3253 1554 420 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6232 Fragments arranged by size: 6232 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2177 1186 Size: 991 5466 BseYI C'CCAG_C Cuts at: 2338 5660 6156 2338 Size: 3322 496 2639 Fragments arranged by size: 3322 2639 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3339 1163 Size: 573 1603 4281 Fragments arranged by size: 4281 1603 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3907 4668 5696 1540 Size: 275 186 1906 761 1028 2301 Fragments arranged by size: 2301 1906 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2493 4518 4603 5674 2493 Size: 2025 85 1071 3276 Fragments arranged by size: 3276 2025 1071 85 BsmI GAATG_Cn' Cuts at: 2177 3968 4061 2177 Size: 1791 93 4573 Fragments arranged by size: 4573 1791 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2301 2493 3776 4518 4603 5674 1391 Size: 399 511 192 1283 742 85 1071 2174 Fragments arranged by size: 2174 1283 1071 742 511 399 192 85 BspEI T'CCGG_A Cuts at: 6120 6120 Size: 6457 BspHI T'CATG_A Cuts at: 4346 6076 4346 Size: 1730 4727 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3812 844 Size: 2968 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4344 1394 Size: 429 2521 3507 Fragments arranged by size: 3507 2521 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4952 5126 65 Size: 1183 3704 174 1396 Fragments arranged by size: 3704 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2916 3336 5101 5288 1700 Size: 110 1106 420 1765 187 2869 Fragments arranged by size: 2869 1765 1106 420 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6457 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6457 BssSI C'ACGA_G Cuts at: 4517 5529 4517 Size: 1012 5445 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6457 BstBI TT'CG_AA Cuts at: 2007 5261 2007 Size: 3254 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6457 BstYI r'GATC_y Cuts at: 1972 3787 4154 4539 4556 6008 6094 6106 6452 Size: 1815 367 385 17 1452 86 12 346 Cuts at: 6452 1972 Size: 1977 Fragments arranged by size: 1977 1815 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4948 Fragments arranged by size: 4948 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5718 Fragments arranged by size: 5718 683 56 BtsI GCAGTG_nn' Cuts at: 3973 4767 4787 3973 Size: 794 20 5643 Fragments arranged by size: 5643 794 20 ClaI AT'CG_AT Cuts at: 1554 3085 1554 Size: 1531 4926 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6422 DraI TTT'AAA Cuts at: 2589 2746 3761 2589 Size: 157 1015 5285 Fragments arranged by size: 5285 1015 157 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5464 809 Size: 507 4148 1802 Fragments arranged by size: 4148 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2142 2372 3904 Size: 54 1425 50 275 18 312 230 1532 Cuts at: 3904 4793 6422 8 Size: 889 1629 43 Fragments arranged by size: 1629 1532 1425 889 312 275 230 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3904 1537 Size: 275 2092 4090 Fragments arranged by size: 4090 2092 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2306 4387 1353 Size: 953 2081 3423 Fragments arranged by size: 3423 2081 953 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5044 5418 5564 387 Size: 1064 138 3455 374 146 1280 Fragments arranged by size: 3455 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2180 2303 2808 2976 3060 3144 3228 Size: 393 548 123 505 168 84 84 84 Cuts at: 3228 3312 3396 3480 3730 4520 5117 5904 6172 Size: 84 84 84 250 790 597 787 268 Cuts at: 6172 1239 Size: 1524 Fragments arranged by size: 1524 790 787 597 548 505 393 268 250 168 123 84 84 84 84 84 84 EcoRI G'AATT_C Cuts at: 2150 2694 2710 2778 3198 3450 3766 4144 2150 Size: 544 16 68 420 252 316 378 4463 Fragments arranged by size: 4463 544 420 378 316 252 68 16 FspI TGC'GCA Cuts at: 4963 4963 Size: 6457 HaeII r_GCGC'y Cuts at: 1678 5604 1678 Size: 3926 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 5098 5130 5172 5204 1373 Size: 32 135 32 3526 32 42 32 2626 Fragments arranged by size: 3526 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2684 3801 4047 669 Size: 988 281 746 1117 246 3079 Fragments arranged by size: 3079 1117 988 746 281 246 HindIII A'AGCT_T Cuts at: 748 2739 2823 2991 3075 3327 3411 3495 748 Size: 1991 84 168 84 252 84 84 3710 Fragments arranged by size: 3710 1991 252 168 84 84 84 84 HpaI GTT'AAC Cuts at: 4047 4047 Size: 6457 KpnI G_GTAC'C Cuts at: 3782 3782 Size: 6457 MfeI C'AATT_G Cuts at: 4056 4056 Size: 6457 MluI A'CGCG_T Cuts at: 6333 6333 Size: 6457 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5571 5755 1305 Size: 4266 184 2007 Fragments arranged by size: 4266 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2144 2374 10 Size: 54 1425 343 312 230 4093 Fragments arranged by size: 4093 1425 343 312 230 54 MslI CAynn'nnrTG Cuts at: 518 1166 2887 4415 4774 4933 518 Size: 648 1721 1528 359 159 2042 Fragments arranged by size: 2042 1721 1528 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3873 4550 5698 5943 1606 Size: 2267 677 1148 245 2120 Fragments arranged by size: 2267 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4948 Fragments arranged by size: 4948 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6457 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6457 NotI GC'GGCC_GC Cuts at: 3904 3904 Size: 6457 NspI r_CATG'y Cuts at: 1132 1229 2668 3815 5360 1132 Size: 97 1439 1147 1545 2229 Fragments arranged by size: 2229 1545 1439 1147 97 PasI CC'CwG_GG Cuts at: 1348 2075 1348 Size: 727 5730 PciI A'CATG_T Cuts at: 5356 5356 Size: 6457 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6457 PmeI GTTT'AAAC Cuts at: 3761 3761 Size: 6457 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4526 4558 6039 6071 4526 Size: 32 1481 32 4912 Fragments arranged by size: 4912 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6457 PsiI TTA'TAA Cuts at: 2505 4027 2505 Size: 1522 4935 PstI C_TGCA'G Cuts at: 830 3809 830 Size: 2979 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6457 SalI G'TCGA_C Cuts at: 1936 3799 1936 Size: 1863 4594 SbfI CC_TGCA'GG Cuts at: 3809 3809 Size: 6457 ScaI AGT'ACT Cuts at: 4705 6417 4705 Size: 1712 4745 SexAI A'CCwGG_T Cuts at: 2232 2232 Size: 6457 SfcI C'TryA_G Cuts at: 826 1045 3805 4940 5621 5812 826 Size: 219 2760 1135 681 191 1471 Fragments arranged by size: 2760 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6457 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6457 SmaI CCC'GGG Cuts at: 1872 3784 1872 Size: 1912 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2054 4561 5462 5724 820 Size: 197 926 111 2507 901 262 1553 Fragments arranged by size: 2507 1553 926 901 262 197 111 SnaBI TAC'GTA Cuts at: 493 3754 4248 493 Size: 3261 494 2702 Fragments arranged by size: 3261 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6457 SphI G_CATG'C Cuts at: 1132 2668 3815 1132 Size: 1536 1147 3774 Fragments arranged by size: 3774 1536 1147 SspI AAT'ATT Cuts at: 5 52 2547 4381 5 Size: 47 2495 1834 2081 Fragments arranged by size: 2495 2081 1834 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6457 StyI C'CwwG_G Cuts at: 513 1065 2022 3896 513 Size: 552 957 1874 3074 Fragments arranged by size: 3074 1874 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2234 4484 4501 4654 4839 5284 325 Size: 1255 654 2250 17 153 185 445 1498 Fragments arranged by size: 2250 1498 1255 654 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3017 4703 6415 Size: 274 80 33 51 1443 1040 1686 1712 Cuts at: 6415 96 Size: 138 Fragments arranged by size: 1712 1686 1443 1040 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6417 6449 6417 Size: 32 6425 Tth111I GACn'n_nGTC Cuts at: 1316 2270 1316 Size: 954 5503 XbaI T'CTAG_A Cuts at: 3793 3793 Size: 6457 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6457 XmaI C'CCGG_G Cuts at: 1870 3782 1870 Size: 1912 4545 XmnI GAAnn'nnTTC Cuts at: 2630 2798 4586 2630 Size: 168 1788 4501 Fragments arranged by size: 4501 1788 168 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4265 276 Size: 53 83 186 936 2731 2468 Fragments arranged by size: 2731 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI