(Circular) (Six-base) MAPSORT of: pFN21ASDA1963.seq Check: 2108 from: 1 to: 5698 pFN21ASDA1963 5698 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2448 2448 Size: 5698 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2991 3041 1937 Size: 1054 50 4594 Fragments arranged by size: 4594 1054 50 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3761 5145 5413 1632 Size: 2129 1384 268 1917 Fragments arranged by size: 2129 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 2331 4597 5574 1203 Size: 1128 2266 977 1327 Fragments arranged by size: 2266 1327 1128 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2129 2456 2914 1184 Size: 945 327 458 3968 Fragments arranged by size: 3968 945 458 327 AlwNI CAG_nnn'CTG Cuts at: 3441 5013 3441 Size: 1572 4126 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 2474 3023 1870 Size: 73 531 549 4545 Fragments arranged by size: 4545 549 531 73 BamHI G'GATC_C Cuts at: 2067 2361 2819 3028 3395 2067 Size: 294 458 209 367 4370 Fragments arranged by size: 4370 458 367 294 209 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2168 2532 3019 4474 618 Size: 325 217 627 381 364 487 1455 1842 Fragments arranged by size: 1842 1455 627 487 381 364 325 217 BanII G_rGCy'C Cuts at: 1391 2604 3017 1391 Size: 1213 413 4072 Fragments arranged by size: 4072 1213 413 BbeI G_GCGC'C Cuts at: 2172 2172 Size: 5698 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2765 928 Size: 971 866 3861 Fragments arranged by size: 3861 971 866 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2166 4309 136 Size: 107 122 71 631 605 494 2143 1525 Fragments arranged by size: 2143 1525 631 605 494 122 107 71 BglII A'GATC_T Cuts at: 5693 5693 Size: 5698 BlpI GC'TnA_GC Cuts at: 3115 3115 Size: 5698 Bme1580I G_kGCm'C Cuts at: 1790 2118 2451 3759 4915 1790 Size: 328 333 1308 1156 2573 Fragments arranged by size: 2573 1308 1156 333 328 BmgBI CAC'GTC Cuts at: 1318 2095 1318 Size: 777 4921 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3429 4387 442 Size: 416 53 83 399 24 2012 958 1753 Fragments arranged by size: 2012 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5698 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2618 2743 4358 1239 Size: 1379 125 1615 2579 Fragments arranged by size: 2579 1615 1379 125 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3823 4688 4986 3823 Size: 865 298 4535 Fragments arranged by size: 4535 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2638 4361 882 Size: 617 1139 1723 2219 Fragments arranged by size: 2219 1723 1139 617 BsaAI yAC'GTr Cuts at: 493 1204 2446 2995 3489 493 Size: 711 1242 549 494 2702 Fragments arranged by size: 2702 1242 711 549 494 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2169 3505 3887 4522 Size: 53 83 186 936 636 1336 382 635 Cuts at: 4522 275 Size: 1451 Fragments arranged by size: 1451 1336 936 636 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4131 4529 4803 4950 5361 4131 Size: 398 274 147 411 4468 Fragments arranged by size: 4468 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2166 2196 1178 Size: 30 165 30 763 30 4680 Fragments arranged by size: 4680 763 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5698 BseYI C'CCAG_C Cuts at: 2429 2823 4901 5397 2429 Size: 394 2078 496 2730 Fragments arranged by size: 2730 2078 496 394 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2321 2339 2426 2884 1163 Size: 573 585 18 87 458 3977 Fragments arranged by size: 3977 585 573 458 87 18 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2234 3148 3909 4937 1540 Size: 275 186 233 914 761 1028 2301 Fragments arranged by size: 2301 1028 914 761 275 233 186 BsiHKAI G_wGCw'C Cuts at: 2565 2604 3759 3844 4915 2565 Size: 39 1155 85 1071 3348 Fragments arranged by size: 3348 1155 1071 85 39 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2118 2451 2565 2604 3017 3759 3844 Size: 399 328 333 114 39 413 742 85 Cuts at: 3844 4915 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 413 399 333 328 114 85 39 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 3587 5317 3587 Size: 1730 3968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2448 3053 844 Size: 1604 605 3489 Fragments arranged by size: 3489 1604 605 BsrBI CCG'CTC Cuts at: 1394 1823 2122 2182 3585 1394 Size: 429 299 60 1403 3507 Fragments arranged by size: 3507 1403 429 299 60 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2717 4342 4529 1700 Size: 110 907 1625 187 2869 Fragments arranged by size: 2869 1625 907 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 2384 2485 2545 2547 1925 Size: 459 101 60 2 5076 Fragments arranged by size: 5076 459 101 60 2 BssSI C'ACGA_G Cuts at: 2912 3758 4770 2912 Size: 846 1012 3840 Fragments arranged by size: 3840 1012 846 BstAPI GCAn_nnn'nTGC Cuts at: 794 2762 794 Size: 1968 3730 BstBI TT'CG_AA Cuts at: 2007 4502 2007 Size: 2495 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstXI CCAn_nnnn'nTGG Cuts at: 2752 2752 Size: 5698 BstYI r'GATC_y Cuts at: 1972 2067 2361 2819 3028 3395 3780 3797 5249 Size: 95 294 458 209 367 385 17 1452 Cuts at: 5249 5335 5347 5693 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 458 385 367 346 294 209 95 86 17 12 BstZ17I GTA'TAC Cuts at: 2992 2992 Size: 5698 BtgI C'CryG_G Cuts at: 513 1065 2161 2456 513 Size: 552 1096 295 3755 Fragments arranged by size: 3755 1096 552 295 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4959 Fragments arranged by size: 4959 683 56 BtsI GCAGTG_nn' Cuts at: 3214 4008 4028 3214 Size: 794 20 4884 Fragments arranged by size: 4884 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2231 2325 3145 Size: 54 1425 50 275 18 401 94 820 Cuts at: 3145 4034 5663 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 820 401 275 94 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2231 3145 1537 Size: 275 419 914 4090 Fragments arranged by size: 4090 914 419 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2570 2609 3628 1353 Size: 1217 39 1019 3423 Fragments arranged by size: 3423 1217 1019 39 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2327 4285 4659 4805 387 Size: 1064 138 738 1958 374 146 1280 Fragments arranged by size: 1958 1280 1064 738 374 146 138 EcoICRI GAG'CTC Cuts at: 2602 2602 Size: 5698 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2618 2743 3761 4358 5145 5413 1239 Size: 393 986 125 1018 597 787 268 1524 Fragments arranged by size: 1524 1018 986 787 597 393 268 125 EcoNI CCTnn'n_nnAGG Cuts at: 2369 2369 Size: 5698 EcoO109I rG'GnC_Cy Cuts at: 2208 2208 Size: 5698 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 2172 2464 2671 4845 1678 Size: 494 292 207 2174 2531 Fragments arranged by size: 2531 2174 494 292 207 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2166 2198 4339 4371 4413 Size: 32 135 32 594 32 2141 32 42 Cuts at: 4413 4445 1373 Size: 32 2626 Fragments arranged by size: 2626 2141 594 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3042 3288 669 Size: 988 281 1104 246 3079 Fragments arranged by size: 3079 1104 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5698 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KasI G'GCGC_C Cuts at: 2168 2168 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 3297 3297 Size: 5698 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4812 4996 1305 Size: 3507 184 2007 Fragments arranged by size: 3507 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2327 10 Size: 54 1425 343 495 3381 Fragments arranged by size: 3381 1425 495 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2084 2750 3656 4015 4174 518 Size: 648 918 666 906 359 159 2042 Fragments arranged by size: 2042 918 906 666 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2163 2257 2353 2458 2683 3114 3791 4939 Size: 557 94 96 105 225 431 677 1148 Cuts at: 4939 5184 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 557 431 245 225 105 96 94 NarI GG'CG_CC Cuts at: 2169 2169 Size: 5698 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5146 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5698 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 3056 4601 1132 Size: 97 1827 1545 2229 Fragments arranged by size: 2229 1827 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5698 PfoI T'CCnGG_A Cuts at: 2607 2607 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PmlI CAC'GTG Cuts at: 2446 2446 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2208 2208 Size: 5698 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PstI C_TGCA'G Cuts at: 830 3050 830 Size: 2220 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2257 2353 2257 Size: 96 5602 SacI G_AGCT'C Cuts at: 2604 2604 Size: 5698 SacII CC_GC'GG Cuts at: 2164 2459 2164 Size: 295 5403 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SanDI GG'GwC_CC Cuts at: 2208 2208 Size: 5698 SapI GCTCTTCn'nnn_ Cuts at: 2570 2609 2570 Size: 39 5659 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SfcI C'TryA_G Cuts at: 826 1045 2287 2590 3046 4181 4862 5053 826 Size: 219 1242 303 456 1135 681 191 1471 Fragments arranged by size: 1471 1242 1135 681 456 303 219 191 SfoI GGC'GCC Cuts at: 2170 2170 Size: 5698 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 2476 3025 1872 Size: 604 549 4545 Fragments arranged by size: 4545 604 549 SmlI C'TyrA_G Cuts at: 820 1017 1943 3802 4703 4965 820 Size: 197 926 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2995 3489 493 Size: 2502 494 2702 Fragments arranged by size: 2702 2502 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SrfI GCCC'GGGC Cuts at: 2476 2476 Size: 5698 SspI AAT'ATT Cuts at: 5 52 3622 5 Size: 47 3570 2081 Fragments arranged by size: 3570 2081 47 StuI AGG'CCT Cuts at: 1517 2737 1517 Size: 1220 4478 StyI C'CwwG_G Cuts at: 513 1065 3137 513 Size: 552 2072 3074 Fragments arranged by size: 3074 2072 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3725 3742 3895 4080 4525 325 Size: 1255 2145 17 153 185 445 1498 Fragments arranged by size: 2145 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3944 5656 96 Size: 274 80 33 51 1443 1967 1712 138 Fragments arranged by size: 1967 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5658 5690 5658 Size: 32 5666 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5698 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 2474 3023 1870 Size: 604 549 4545 Fragments arranged by size: 4545 604 549 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SexAI SfiI SwaI XcmI