(Circular) (Six-base) MAPSORT of: pFN21ASDA1959.seq Check: 9182 from: 1 to: 6676 pFN21ASDA1959 6676 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2730 4486 278 Size: 53 83 186 936 1194 1756 2468 Fragments arranged by size: 2468 1756 1194 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4019 1937 Size: 2082 4594 Acc65I G'GTAC_C Cuts at: 3997 3997 Size: 6676 AclI AA'CG_TT Cuts at: 1563 4803 5176 1563 Size: 3240 373 3063 Fragments arranged by size: 3240 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3767 4739 6123 6391 1632 Size: 2135 972 1384 268 1917 Fragments arranged by size: 2135 1917 1384 972 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6479 AflIII A'CryG_T Cuts at: 1203 2656 5575 6552 1203 Size: 1453 2919 977 1327 Fragments arranged by size: 2919 1453 1327 977 AgeI A'CCGG_T Cuts at: 5507 5507 Size: 6676 AhdI GACnn_n'nnGTC Cuts at: 5405 5405 Size: 6676 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6676 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6676 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2202 2234 2202 Size: 32 6644 AlwNI CAG_nnn'CTG Cuts at: 2481 2609 2988 3139 3790 4419 5991 2481 Size: 128 379 151 651 629 1572 3166 Fragments arranged by size: 3166 1572 651 629 379 151 128 ApaLI G'TGCA_C Cuts at: 4733 5889 4733 Size: 1156 5520 ApoI r'AATT_y Cuts at: 1493 3985 4211 4363 6472 1493 Size: 2492 226 152 2109 1697 Fragments arranged by size: 2492 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5230 160 Size: 872 4198 1606 Fragments arranged by size: 4198 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 AvaI C'yCGr_G Cuts at: 1870 1943 2283 2766 2852 3092 4001 1870 Size: 73 340 483 86 240 909 4545 Fragments arranged by size: 4545 909 483 340 240 86 73 BamHI G'GATC_C Cuts at: 4006 4373 4006 Size: 367 6309 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2120 3997 5452 618 Size: 325 217 627 333 1877 1455 1842 Fragments arranged by size: 1877 1842 1455 627 333 325 217 BanII G_rGCy'C Cuts at: 1391 2179 2520 3093 3513 3995 1391 Size: 788 341 573 420 482 4072 Fragments arranged by size: 4072 788 573 482 420 341 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2565 928 Size: 971 666 5039 Fragments arranged by size: 5039 971 666 BbvCI CC'TCA_GC Cuts at: 3140 3300 3140 Size: 160 6516 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3317 3351 4867 4901 1903 Size: 34 1380 34 1516 34 3678 Fragments arranged by size: 3678 1516 1380 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3558 4568 5778 3558 Size: 1010 1210 4456 Fragments arranged by size: 4456 1210 1010 BclI T'GATC_A Cuts at: 1548 1548 Size: 6676 BfrBI ATG'CAT Cuts at: 3360 3360 Size: 6676 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3159 5287 136 Size: 107 122 71 631 605 1487 2128 1525 Fragments arranged by size: 2128 1525 1487 631 605 122 107 71 BglII A'GATC_T Cuts at: 2361 6671 2361 Size: 4310 2366 BlpI GC'TnA_GC Cuts at: 4093 4093 Size: 6676 Bme1580I G_kGCm'C Cuts at: 1790 4737 5893 1790 Size: 2947 1156 2573 Fragments arranged by size: 2947 2573 1156 BmgBI CAC'GTC Cuts at: 1318 2149 2919 1318 Size: 831 770 5075 Fragments arranged by size: 5075 831 770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2037 2755 3232 Size: 416 53 83 399 24 620 718 477 Cuts at: 3232 3942 4407 5365 442 Size: 710 465 958 1753 Fragments arranged by size: 1753 958 718 710 620 477 465 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6676 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2534 2861 3967 5336 1239 Size: 1295 327 1106 1369 2579 Fragments arranged by size: 2579 1369 1295 1106 327 Bpu10I CC'TnA_GC Cuts at: 2320 3140 3300 2320 Size: 820 160 5696 Fragments arranged by size: 5696 820 160 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2561 4801 5666 5964 2561 Size: 2240 865 298 3273 Fragments arranged by size: 3273 2240 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3947 5339 882 Size: 617 2448 1392 2219 Fragments arranged by size: 2448 2219 1392 617 BsaAI yAC'GTr Cuts at: 493 1204 3973 4467 493 Size: 711 2769 494 2702 Fragments arranged by size: 2769 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6670 6670 Size: 6676 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2727 4483 4865 5500 Size: 53 83 186 936 1194 1756 382 635 Cuts at: 5500 275 Size: 1451 Fragments arranged by size: 1756 1451 1194 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5109 5507 5781 5928 6339 5109 Size: 398 274 147 411 5446 Fragments arranged by size: 5446 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3298 3328 1178 Size: 30 165 30 1895 30 4526 Fragments arranged by size: 4526 1895 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2084 2144 3096 1186 Size: 898 60 952 4766 Fragments arranged by size: 4766 952 898 60 BseYI C'CCAG_C Cuts at: 3542 5879 6375 3542 Size: 2337 496 3843 Fragments arranged by size: 3843 2337 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3438 1163 Size: 573 1702 4401 Fragments arranged by size: 4401 1702 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4126 4887 5915 1540 Size: 275 186 2125 761 1028 2301 Fragments arranged by size: 2301 2125 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2154 2520 3551 4737 4822 5893 2154 Size: 366 1031 1186 85 1071 2937 Fragments arranged by size: 2937 1186 1071 1031 366 85 BsmI GAATG_Cn' Cuts at: 2984 4187 4280 2984 Size: 1203 93 5380 Fragments arranged by size: 5380 1203 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3092 3092 Size: 6676 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2154 2179 2520 3093 3513 3551 3995 Size: 399 364 25 341 573 420 38 444 Cuts at: 3995 4737 4822 5893 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 573 444 420 399 364 341 85 38 25 BspEI T'CCGG_A Cuts at: 6339 6339 Size: 6676 BspHI T'CATG_A Cuts at: 4565 6295 4565 Size: 1730 4946 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3565 3782 4031 844 Size: 2721 217 249 3489 Fragments arranged by size: 3489 2721 249 217 BsrBI CCG'CTC Cuts at: 1394 1823 2050 4563 1394 Size: 429 227 2513 3507 Fragments arranged by size: 3507 2513 429 227 BsrDI GCAATG_nn' Cuts at: 65 1248 5171 5345 65 Size: 1183 3923 174 1396 Fragments arranged by size: 3923 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2312 2930 5320 5507 1700 Size: 110 502 618 2390 187 2869 Fragments arranged by size: 2869 2390 618 502 187 110 BsrGI T'GTAC_A Cuts at: 96 2076 96 Size: 1980 4696 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6676 BssSI C'ACGA_G Cuts at: 4736 5748 4736 Size: 1012 5664 BstAPI GCAn_nnn'nTGC Cuts at: 794 2609 794 Size: 1815 4861 BstBI TT'CG_AA Cuts at: 2007 5480 2007 Size: 3473 3203 BstEII G'GTnAC_C Cuts at: 1184 2255 1184 Size: 1071 5605 BstYI r'GATC_y Cuts at: 1972 2361 2907 3498 3834 3898 4006 4373 4758 Size: 389 546 591 336 64 108 367 385 Cuts at: 4758 4775 6227 6313 6325 6671 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 591 546 389 385 367 346 336 108 86 64 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2160 2202 2469 3922 513 Size: 552 957 138 42 267 1453 3267 Fragments arranged by size: 3267 1453 957 552 267 138 42 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3187 487 Size: 683 56 1961 3976 Fragments arranged by size: 3976 1961 683 56 BtsI GCAGTG_nn' Cuts at: 4192 4986 5006 4192 Size: 794 20 5862 Fragments arranged by size: 5862 794 20 ClaI AT'CG_AT Cuts at: 1554 3411 1554 Size: 1857 4819 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3357 3392 529 Size: 35 2793 35 3813 Fragments arranged by size: 3813 2793 35 35 DraI TTT'AAA Cuts at: 3980 3980 Size: 6676 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5683 809 Size: 507 4367 1802 Fragments arranged by size: 4367 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2035 4123 5012 Size: 54 1425 50 275 18 205 2088 889 Cuts at: 5012 6641 8 Size: 1629 43 Fragments arranged by size: 2088 1629 1425 889 275 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4123 1537 Size: 275 2311 4090 Fragments arranged by size: 4090 2311 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4606 1353 Size: 3253 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5263 5637 5783 387 Size: 1064 138 3674 374 146 1280 Fragments arranged by size: 3674 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2518 2518 Size: 6676 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2534 2861 3767 3967 4739 5336 6123 Size: 393 902 327 906 200 772 597 787 Cuts at: 6123 6391 1239 Size: 268 1524 Fragments arranged by size: 1524 906 902 787 772 597 393 327 268 200 EcoO109I rG'GnC_Cy Cuts at: 2572 3787 2572 Size: 1215 5461 EcoRI G'AATT_C Cuts at: 3985 4363 3985 Size: 378 6298 EcoRV GAT'ATC Cuts at: 2773 2773 Size: 6676 FspI TGC'GCA Cuts at: 5182 5182 Size: 6676 HaeII r_GCGC'y Cuts at: 1678 2116 2144 5823 1678 Size: 438 28 3679 2531 Fragments arranged by size: 3679 2531 438 28 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2597 2629 3091 3123 3826 Size: 32 135 32 1025 32 462 32 703 Cuts at: 3826 3858 5317 5349 5391 5423 1373 Size: 32 1459 32 42 32 2626 Fragments arranged by size: 2626 1459 1025 703 462 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3148 4020 4266 669 Size: 988 281 1210 872 246 3079 Fragments arranged by size: 3079 1210 988 872 281 246 HindIII A'AGCT_T Cuts at: 748 2330 748 Size: 1582 5094 HpaI GTT'AAC Cuts at: 4266 4266 Size: 6676 KpnI G_GTAC'C Cuts at: 4001 4001 Size: 6676 MfeI C'AATT_G Cuts at: 4275 4275 Size: 6676 MluI A'CGCG_T Cuts at: 6552 6552 Size: 6676 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2361 2556 2885 3057 5790 5974 1305 Size: 1056 195 329 172 2733 184 2007 Fragments arranged by size: 2733 2007 1056 329 195 184 172 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4854 Fragments arranged by size: 4854 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2253 2867 3347 4634 4993 5152 518 Size: 648 1087 614 480 1287 359 159 2042 Fragments arranged by size: 2042 1287 1087 648 614 480 359 159 MspA1I CmG'CkG Cuts at: 1606 2506 3139 3304 3892 4092 4769 5917 6162 Size: 900 633 165 588 200 677 1148 245 Cuts at: 6162 1606 Size: 2120 Fragments arranged by size: 2120 1148 900 677 633 588 245 200 165 NcoI C'CATG_G Cuts at: 513 1065 2022 2160 3922 513 Size: 552 957 138 1762 3267 Fragments arranged by size: 3267 1762 957 552 138 NdeI CA'TA_TG Cuts at: 387 387 Size: 6676 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6676 NotI GC'GGCC_GC Cuts at: 4123 4123 Size: 6676 NsiI A_TGCA'T Cuts at: 3362 3362 Size: 6676 NspI r_CATG'y Cuts at: 1132 1229 2232 2633 2660 3286 3364 4034 5579 Size: 97 1003 401 27 626 78 670 1545 Cuts at: 5579 1132 Size: 2229 Fragments arranged by size: 2229 1545 1003 670 626 401 97 78 27 PasI CC'CwG_GG Cuts at: 1348 2449 1348 Size: 1101 5575 PciI A'CATG_T Cuts at: 2656 5575 2656 Size: 2919 3757 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6676 PfoI T'CCnGG_A Cuts at: 2265 2265 Size: 6676 PmeI GTTT'AAAC Cuts at: 3980 3980 Size: 6676 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2011 2043 4745 4777 6258 6290 2011 Size: 32 2702 32 1481 32 2397 Fragments arranged by size: 2702 2397 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2572 2572 Size: 6676 PshAI GACnn'nnGTC Cuts at: 1613 2471 1613 Size: 858 5818 PsiI TTA'TAA Cuts at: 4246 4246 Size: 6676 PstI C_TGCA'G Cuts at: 830 2466 2605 3589 4028 830 Size: 1636 139 984 439 3478 Fragments arranged by size: 3478 1636 984 439 139 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6676 PvuII CAG'CTG Cuts at: 3892 3892 Size: 6676 SacI G_AGCT'C Cuts at: 2520 2520 Size: 6676 SalI G'TCGA_C Cuts at: 1936 4018 1936 Size: 2082 4594 SbfI CC_TGCA'GG Cuts at: 4028 4028 Size: 6676 ScaI AGT'ACT Cuts at: 3227 4924 6636 3227 Size: 1697 1712 3267 Fragments arranged by size: 3267 1712 1697 SfcI C'TryA_G Cuts at: 826 1045 2462 2601 3585 3613 4024 5159 5840 Size: 219 1417 139 984 28 411 1135 681 Cuts at: 5840 6031 826 Size: 191 1471 Fragments arranged by size: 1471 1417 1135 984 681 411 219 191 139 28 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6676 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6676 SmaI CCC'GGG Cuts at: 1872 2285 2854 4003 1872 Size: 413 569 1149 4545 Fragments arranged by size: 4545 1149 569 413 SmlI C'TyrA_G Cuts at: 820 1017 1943 2576 4780 5681 5943 820 Size: 197 926 633 2204 901 262 1553 Fragments arranged by size: 2204 1553 926 901 633 262 197 SnaBI TAC'GTA Cuts at: 493 3973 4467 493 Size: 3480 494 2702 Fragments arranged by size: 3480 2702 494 SpeI A'CTAG_T Cuts at: 152 3373 152 Size: 3221 3455 SphI G_CATG'C Cuts at: 1132 3364 4034 1132 Size: 2232 670 3774 Fragments arranged by size: 3774 2232 670 SspI AAT'ATT Cuts at: 5 52 4600 5 Size: 47 4548 2081 Fragments arranged by size: 4548 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6676 StyI C'CwwG_G Cuts at: 513 1065 2022 2160 2193 2967 3807 3922 4115 Size: 552 957 138 33 774 840 115 193 Cuts at: 4115 513 Size: 3074 Fragments arranged by size: 3074 957 840 774 552 193 138 115 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2737 3368 4703 4720 4873 5058 5503 Size: 1255 1157 631 1335 17 153 185 445 Cuts at: 5503 325 Size: 1498 Fragments arranged by size: 1498 1335 1255 1157 631 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2076 3225 4922 Size: 274 80 33 51 1443 99 1149 1697 Cuts at: 4922 6634 96 Size: 1712 138 Fragments arranged by size: 1712 1697 1443 1149 274 138 99 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3740 3772 6636 6668 3740 Size: 32 2864 32 3748 Fragments arranged by size: 3748 2864 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6676 XbaI T'CTAG_A Cuts at: 4012 4012 Size: 6676 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3929 3929 Size: 6676 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6676 XmaI C'CCGG_G Cuts at: 1870 2283 2852 4001 1870 Size: 413 569 1149 4545 Fragments arranged by size: 4545 1149 569 413 XmnI GAAnn'nnTTC Cuts at: 4805 4805 Size: 6676 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2728 4484 276 Size: 53 83 186 936 1194 1756 2468 Fragments arranged by size: 2468 1756 1194 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI