(Circular) (Six-base) MAPSORT of: pFN21ASDA1949.seq Check: 6537 from: 1 to: 6568 pFN21ASDA1949 6568 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4378 278 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2686 3911 1937 Size: 749 1225 4594 Fragments arranged by size: 4594 1225 749 Acc65I G'GTAC_C Cuts at: 3537 3889 3537 Size: 352 6216 AclI AA'CG_TT Cuts at: 1563 4695 5068 1563 Size: 3132 373 3063 Fragments arranged by size: 3132 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3631 4631 6015 6283 1632 Size: 1999 1000 1384 268 1917 Fragments arranged by size: 1999 1917 1384 1000 268 AflII C'TTAA_G Cuts at: 820 1017 3635 820 Size: 197 2618 3753 Fragments arranged by size: 3753 2618 197 AflIII A'CryG_T Cuts at: 1203 5467 6444 1203 Size: 4264 977 1327 Fragments arranged by size: 4264 1327 977 AgeI A'CCGG_T Cuts at: 5399 5399 Size: 6568 AhdI GACnn_n'nnGTC Cuts at: 5297 5297 Size: 6568 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6568 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6568 AlwNI CAG_nnn'CTG Cuts at: 4311 5883 4311 Size: 1572 4996 ApaI G_GGCC'C Cuts at: 3415 3716 3415 Size: 301 6267 ApaLI G'TGCA_C Cuts at: 2276 4625 5781 2276 Size: 2349 1156 3063 Fragments arranged by size: 3063 2349 1156 ApoI r'AATT_y Cuts at: 1493 3877 4103 4255 6364 1493 Size: 2384 226 152 2109 1697 Fragments arranged by size: 2384 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5122 160 Size: 872 4090 1606 Fragments arranged by size: 4090 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 AvaI C'yCGr_G Cuts at: 1870 1943 2379 2529 2649 3045 3517 3893 1870 Size: 73 436 150 120 396 472 376 4545 Fragments arranged by size: 4545 472 436 396 376 150 120 73 BamHI G'GATC_C Cuts at: 3358 3898 4265 3358 Size: 540 367 5661 Fragments arranged by size: 5661 540 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2064 3537 3889 5344 618 Size: 325 217 627 277 1473 352 1455 1842 Fragments arranged by size: 1842 1473 1455 627 352 325 277 217 BanII G_rGCy'C Cuts at: 1391 2151 2423 2500 2573 2696 3178 3199 3291 Size: 760 272 77 73 123 482 21 92 Cuts at: 3291 3415 3716 3810 3887 1391 Size: 124 301 94 77 4072 Fragments arranged by size: 4072 760 482 301 272 124 123 94 92 77 77 73 21 BbeI G_GCGC'C Cuts at: 2068 2068 Size: 6568 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5597 BbvCI CC'TCA_GC Cuts at: 2810 2810 Size: 6568 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4759 4793 1903 Size: 34 2822 34 3678 Fragments arranged by size: 3678 2822 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4460 5670 4460 Size: 1210 5358 BclI T'GATC_A Cuts at: 1548 1548 Size: 6568 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5179 136 Size: 107 122 71 631 605 3507 1525 Fragments arranged by size: 3507 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3555 3639 6563 3555 Size: 84 2924 3560 Fragments arranged by size: 3560 2924 84 BlpI GC'TnA_GC Cuts at: 3985 3985 Size: 6568 Bme1580I G_kGCm'C Cuts at: 1790 2280 3222 3415 3465 3716 3739 4629 5785 Size: 490 942 193 50 251 23 890 1156 Cuts at: 5785 1790 Size: 2573 Fragments arranged by size: 2573 1156 942 890 490 251 193 50 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6568 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3726 4299 5257 Size: 416 53 83 399 24 2309 573 958 Cuts at: 5257 442 Size: 1753 Fragments arranged by size: 2309 1753 958 573 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2060 2351 1056 Size: 1004 291 5273 Fragments arranged by size: 5273 1004 291 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2174 2214 2279 2393 2543 2557 2604 2631 Size: 935 40 65 114 150 14 47 27 Cuts at: 2631 2740 2810 3081 3162 3273 3275 3477 3683 Size: 109 70 271 81 111 2 202 206 Cuts at: 3683 3722 3732 3794 5228 1239 Size: 39 10 62 1434 2579 Fragments arranged by size: 2579 1434 935 271 206 202 150 114 111 109 81 70 65 62 47 40 39 27 14 10 2 Bpu10I CC'TnA_GC Cuts at: 2810 3177 2810 Size: 367 6201 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4693 5558 5856 4693 Size: 865 298 5405 Fragments arranged by size: 5405 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2383 2459 2740 2906 3659 5231 882 Size: 617 884 76 281 166 753 1572 2219 Fragments arranged by size: 2219 1572 884 753 617 281 166 76 BsaAI yAC'GTr Cuts at: 493 1204 3672 3865 4359 493 Size: 711 2468 193 494 2702 Fragments arranged by size: 2702 2468 711 494 193 BsaBI GATnn'nnATC Cuts at: 6562 6562 Size: 6568 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2065 2081 4375 4757 Size: 53 83 186 936 532 16 2294 382 Cuts at: 4757 5392 275 Size: 635 1451 Fragments arranged by size: 2294 1451 936 635 532 382 186 83 53 16 BsaWI w'CCGG_w Cuts at: 5001 5399 5673 5820 6231 5001 Size: 398 274 147 411 5338 Fragments arranged by size: 5338 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2066 2096 2105 2133 2135 Size: 30 165 30 663 30 9 28 2 Cuts at: 2135 2163 2555 2585 2590 2620 3656 3686 1178 Size: 28 392 30 5 30 1036 30 4060 Fragments arranged by size: 4060 1036 663 392 165 30 30 30 30 30 30 28 28 9 5 2 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 2491 3179 3566 3719 1186 Size: 904 401 688 387 153 4035 Fragments arranged by size: 4035 904 688 401 387 153 BseYI C'CCAG_C Cuts at: 2393 2482 3245 3328 3475 3524 3709 5771 6267 Size: 89 763 83 147 49 185 2062 496 Cuts at: 6267 2393 Size: 2694 Fragments arranged by size: 2694 2062 763 496 185 147 89 83 49 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5995 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4018 4779 5807 1540 Size: 275 186 2017 761 1028 2301 Fragments arranged by size: 2301 2017 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2280 2573 3291 3810 4629 4714 5785 2280 Size: 293 718 519 819 85 1071 3063 Fragments arranged by size: 3063 1071 819 718 519 293 85 BsmI GAATG_Cn' Cuts at: 4079 4172 4079 Size: 93 6475 BsmBI CGTCTCn'nnnn_ Cuts at: 3587 3587 Size: 6568 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2280 2423 2500 2573 2696 3178 Size: 399 361 129 143 77 73 123 482 Cuts at: 3178 3199 3222 3291 3415 3465 3716 3739 3810 Size: 21 23 69 124 50 251 23 71 Cuts at: 3810 3887 4629 4714 5785 1391 Size: 77 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 482 399 361 251 143 129 124 123 85 77 77 73 71 69 50 23 23 21 BspEI T'CCGG_A Cuts at: 6231 6231 Size: 6568 BspHI T'CATG_A Cuts at: 3366 4457 6187 3366 Size: 1091 1730 3747 Fragments arranged by size: 3747 1730 1091 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3923 844 Size: 3079 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2305 2386 2626 4455 1394 Size: 429 482 81 240 1829 3507 Fragments arranged by size: 3507 1829 482 429 240 81 BsrDI GCAATG_nn' Cuts at: 65 1248 5063 5237 65 Size: 1183 3815 174 1396 Fragments arranged by size: 3815 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2067 3855 5212 5399 1700 Size: 110 257 1788 1357 187 2869 Fragments arranged by size: 2869 1788 1357 257 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6568 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6568 BssSI C'ACGA_G Cuts at: 4628 5640 4628 Size: 1012 5556 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6568 BstBI TT'CG_AA Cuts at: 2007 5372 2007 Size: 3365 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6568 BstXI CCAn_nnnn'nTGG Cuts at: 3353 3353 Size: 6568 BstYI r'GATC_y Cuts at: 1972 3358 3555 3639 3898 4265 4650 4667 6119 Size: 1386 197 84 259 367 385 17 1452 Cuts at: 6119 6205 6217 6563 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1386 385 367 346 259 197 86 84 17 12 Bsu36I CC'TnA_GG Cuts at: 2513 3723 2513 Size: 1210 5358 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5059 Fragments arranged by size: 5059 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5829 Fragments arranged by size: 5829 683 56 BtsI GCAGTG_nn' Cuts at: 3288 3430 4084 4878 4898 3288 Size: 142 654 794 20 4958 Fragments arranged by size: 4958 794 654 142 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6568 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6533 DraI TTT'AAA Cuts at: 3872 3872 Size: 6568 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5575 809 Size: 507 4259 1802 Fragments arranged by size: 4259 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2867 4015 Size: 54 1425 50 275 18 195 842 1148 Cuts at: 4015 4904 6533 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1148 889 842 275 195 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4015 1537 Size: 275 2203 4090 Fragments arranged by size: 4090 2203 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2334 2425 2439 2856 3267 3765 3777 4498 Size: 981 91 14 417 411 498 12 721 Cuts at: 4498 1353 Size: 3423 Fragments arranged by size: 3423 981 721 498 417 411 91 14 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5155 5529 5675 387 Size: 1064 138 3566 374 146 1280 Fragments arranged by size: 3566 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2571 3289 3808 2571 Size: 718 519 5331 Fragments arranged by size: 5331 718 519 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2174 2214 2279 2393 2543 2557 2604 Size: 393 542 40 65 114 150 14 47 Cuts at: 2604 2631 2740 2810 3081 3162 3273 3275 3477 Size: 27 109 70 271 81 111 2 202 Cuts at: 3477 3631 3683 3722 3732 3794 4631 5228 6015 Size: 154 52 39 10 62 837 597 787 Cuts at: 6015 6283 1239 Size: 268 1524 Fragments arranged by size: 1524 837 787 597 542 393 271 268 202 154 150 114 111 109 81 70 65 62 52 47 40 39 27 14 10 2 EcoNI CCTnn'n_nnAGG Cuts at: 2205 2258 2729 3602 2205 Size: 53 471 873 5171 Fragments arranged by size: 5171 873 471 53 EcoO109I rG'GnC_Cy Cuts at: 3411 3412 3609 3727 3832 3411 Size: 1 197 118 105 6147 Fragments arranged by size: 6147 197 118 105 1 EcoRI G'AATT_C Cuts at: 3877 4255 3877 Size: 378 6190 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3661 3661 Size: 6568 FspI TGC'GCA Cuts at: 3824 5074 3824 Size: 1250 5318 HaeII r_GCGC'y Cuts at: 1678 2068 2119 2161 5715 1678 Size: 390 51 42 3554 2531 Fragments arranged by size: 3554 2531 390 51 42 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2066 2098 2105 2137 2398 Size: 32 135 32 494 32 7 32 261 Cuts at: 2398 2430 2440 2472 2555 2587 2590 2622 5209 Size: 32 10 32 83 32 3 32 2587 Cuts at: 5209 5241 5283 5315 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2587 494 261 135 83 42 32 32 32 32 32 32 32 32 32 32 10 7 3 HincII GTy'rAC Cuts at: 669 1657 1938 3445 3912 4158 669 Size: 988 281 1507 467 246 3079 Fragments arranged by size: 3079 1507 988 467 281 246 HindIII A'AGCT_T Cuts at: 748 2171 3113 748 Size: 1423 942 4203 Fragments arranged by size: 4203 1423 942 HpaI GTT'AAC Cuts at: 4158 4158 Size: 6568 KasI G'GCGC_C Cuts at: 2064 2064 Size: 6568 KpnI G_GTAC'C Cuts at: 3541 3893 3541 Size: 352 6216 MfeI C'AATT_G Cuts at: 4167 4167 Size: 6568 MluI A'CGCG_T Cuts at: 6444 6444 Size: 6568 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5682 5866 1305 Size: 4377 184 2007 Fragments arranged by size: 4377 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2869 10 Size: 54 1425 343 195 842 3709 Fragments arranged by size: 3709 1425 842 343 195 54 MslI CAynn'nnrTG Cuts at: 518 1166 2593 4526 4885 5044 518 Size: 648 1427 1933 359 159 2042 Fragments arranged by size: 2042 1933 1427 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2053 3594 3984 4661 5809 6054 1606 Size: 447 1541 390 677 1148 245 2120 Fragments arranged by size: 2120 1541 1148 677 447 390 245 NaeI GCC'GGC Cuts at: 2069 3857 2069 Size: 1788 4780 NarI GG'CG_CC Cuts at: 2065 2065 Size: 6568 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5059 Fragments arranged by size: 5059 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6568 NgoMIV G'CCGG_C Cuts at: 2067 3855 2067 Size: 1788 4780 NheI G'CTAG_C Cuts at: 1052 2056 2347 1052 Size: 1004 291 5273 Fragments arranged by size: 5273 1004 291 NotI GC'GGCC_GC Cuts at: 4015 4015 Size: 6568 NspI r_CATG'y Cuts at: 1132 1229 3926 5471 1132 Size: 97 2697 1545 2229 Fragments arranged by size: 2697 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2186 2399 2696 3060 1348 Size: 838 213 297 364 4856 Fragments arranged by size: 4856 838 364 297 213 PciI A'CATG_T Cuts at: 5467 5467 Size: 6568 PflMI CCAn_nnn'nTGG Cuts at: 1108 2719 1108 Size: 1611 4957 PfoI T'CCnGG_A Cuts at: 2151 2608 3035 2151 Size: 457 427 5684 Fragments arranged by size: 5684 457 427 PmeI GTTT'AAAC Cuts at: 3872 3872 Size: 6568 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3197 3229 4637 4669 6150 6182 3197 Size: 32 1408 32 1481 32 3583 Fragments arranged by size: 3583 1481 1408 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3609 3727 3609 Size: 118 6450 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6568 PsiI TTA'TAA Cuts at: 4138 4138 Size: 6568 PspOMI G'GGCC_C Cuts at: 3411 3712 3411 Size: 301 6267 PstI C_TGCA'G Cuts at: 830 2902 3204 3514 3801 3920 830 Size: 2072 302 310 287 119 3478 Fragments arranged by size: 3478 2072 310 302 287 119 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6568 PvuII CAG'CTG Cuts at: 2053 3594 2053 Size: 1541 5027 SacI G_AGCT'C Cuts at: 2573 3291 3810 2573 Size: 718 519 5331 Fragments arranged by size: 5331 718 519 SalI G'TCGA_C Cuts at: 1936 3910 1936 Size: 1974 4594 SanDI GG'GwC_CC Cuts at: 3609 3609 Size: 6568 SapI GCTCTTCn'nnn_ Cuts at: 2334 3777 2334 Size: 1443 5125 SbfI CC_TGCA'GG Cuts at: 3920 3920 Size: 6568 ScaI AGT'ACT Cuts at: 4816 6528 4816 Size: 1712 4856 SfcI C'TryA_G Cuts at: 826 1045 2048 2214 2898 3200 3510 3797 3916 Size: 219 1003 166 684 302 310 287 119 Cuts at: 3916 5051 5732 5923 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1003 684 681 310 302 287 219 191 166 119 SfoI GGC'GCC Cuts at: 2066 2066 Size: 6568 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6568 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6568 SmaI CCC'GGG Cuts at: 1872 2381 3519 3895 1872 Size: 509 1138 376 4545 Fragments arranged by size: 4545 1138 509 376 SmlI C'TyrA_G Cuts at: 820 1017 1943 2649 3045 3635 4672 5573 5835 Size: 197 926 706 396 590 1037 901 262 Cuts at: 5835 820 Size: 1553 Fragments arranged by size: 1553 1037 926 901 706 590 396 262 197 SnaBI TAC'GTA Cuts at: 493 3865 4359 493 Size: 3372 494 2702 Fragments arranged by size: 3372 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6568 SphI G_CATG'C Cuts at: 1132 3926 1132 Size: 2794 3774 SspI AAT'ATT Cuts at: 5 52 4492 5 Size: 47 4440 2081 Fragments arranged by size: 4440 2081 47 StuI AGG'CCT Cuts at: 1517 2518 2974 3187 3258 1517 Size: 1001 456 213 71 4827 Fragments arranged by size: 4827 1001 456 213 71 StyI C'CwwG_G Cuts at: 513 1065 2022 2424 2932 3603 3828 4007 513 Size: 552 957 402 508 671 225 179 3074 Fragments arranged by size: 3074 957 671 552 508 402 225 179 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3073 4595 4612 4765 4950 5395 325 Size: 1255 1493 1522 17 153 185 445 1498 Fragments arranged by size: 1522 1498 1493 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4814 6526 96 Size: 274 80 33 51 1443 2837 1712 138 Fragments arranged by size: 2837 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6528 6560 6528 Size: 32 6536 Tth111I GACn'n_nGTC Cuts at: 1316 2454 1316 Size: 1138 5430 XbaI T'CTAG_A Cuts at: 3904 3904 Size: 6568 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3012 3012 Size: 6568 XhoI C'TCGA_G Cuts at: 1943 2649 3045 1943 Size: 706 396 5466 Fragments arranged by size: 5466 706 396 XmaI C'CCGG_G Cuts at: 1870 2379 3517 3893 1870 Size: 509 1138 376 4545 Fragments arranged by size: 4545 1138 509 376 XmnI GAAnn'nnTTC Cuts at: 2288 4697 2288 Size: 2409 4159 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4376 276 Size: 53 83 186 936 2842 2468 Fragments arranged by size: 2842 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstZ17I DraIII EcoRV FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI