(Circular) (Six-base) MAPSORT of: pFN21ASDA1916.seq Check: 858 from: 1 to: 6364 pFN21ASDA1916 6364 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2098 2098 Size: 6364 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4174 278 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3707 1937 Size: 1770 4594 Acc65I G'GTAC_C Cuts at: 3685 3685 Size: 6364 AclI AA'CG_TT Cuts at: 1563 4491 4864 1563 Size: 2928 373 3063 Fragments arranged by size: 3063 2928 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2464 4427 5811 6079 1632 Size: 832 1963 1384 268 1917 Fragments arranged by size: 1963 1917 1384 832 268 AfeI AGC'GCT Cuts at: 2052 2052 Size: 6364 AflII C'TTAA_G Cuts at: 820 1017 3649 820 Size: 197 2632 3535 Fragments arranged by size: 3535 2632 197 AflIII A'CryG_T Cuts at: 1203 5263 6240 1203 Size: 4060 977 1327 Fragments arranged by size: 4060 1327 977 AgeI A'CCGG_T Cuts at: 5195 5195 Size: 6364 AhdI GACnn_n'nnGTC Cuts at: 5093 5093 Size: 6364 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6364 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6364 AlwNI CAG_nnn'CTG Cuts at: 3121 4107 5679 3121 Size: 986 1572 3806 Fragments arranged by size: 3806 1572 986 ApaLI G'TGCA_C Cuts at: 4421 5577 4421 Size: 1156 5208 ApoI r'AATT_y Cuts at: 1493 2299 2525 2578 2815 2954 2963 3673 3899 Size: 806 226 53 237 139 9 710 226 Cuts at: 3899 4051 6160 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 806 710 237 226 226 152 139 53 9 AseI AT'TA_AT Cuts at: 160 1032 4918 160 Size: 872 3886 1606 Fragments arranged by size: 3886 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 AvaI C'yCGr_G Cuts at: 1870 1943 2054 3351 3405 3689 1870 Size: 73 111 1297 54 284 4545 Fragments arranged by size: 4545 1297 284 111 73 54 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2603 2636 2603 Size: 33 6331 BamHI G'GATC_C Cuts at: 3694 4061 3694 Size: 367 5997 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2078 2185 3547 3685 5140 Size: 325 217 627 291 107 1362 138 1455 Cuts at: 5140 618 Size: 1842 Fragments arranged by size: 1842 1455 1362 627 325 291 217 138 107 BanII G_rGCy'C Cuts at: 1391 2177 3683 1391 Size: 786 1506 4072 Fragments arranged by size: 4072 1506 786 BbeI G_GCGC'C Cuts at: 2082 3551 2082 Size: 1469 4895 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2470 928 Size: 971 571 4822 Fragments arranged by size: 4822 971 571 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3533 3567 4555 4589 1903 Size: 34 1596 34 988 34 3678 Fragments arranged by size: 3678 1596 988 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2716 4256 5466 2716 Size: 1540 1210 3614 Fragments arranged by size: 3614 1540 1210 BclI T'GATC_A Cuts at: 1548 2881 1548 Size: 1333 5031 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4975 136 Size: 107 122 71 631 605 3303 1525 Fragments arranged by size: 3303 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6359 6359 Size: 6364 BlpI GC'TnA_GC Cuts at: 3781 3781 Size: 6364 Bme1580I G_kGCm'C Cuts at: 1790 2190 4425 5581 1790 Size: 400 2235 1156 2573 Fragments arranged by size: 2573 2235 1156 400 BmgBI CAC'GTC Cuts at: 1318 2644 1318 Size: 1326 5038 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4095 5053 442 Size: 416 53 83 399 24 2678 958 1753 Fragments arranged by size: 2678 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6364 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2813 3203 5024 1239 Size: 1574 390 1821 2579 Fragments arranged by size: 2579 1821 1574 390 Bpu10I CC'TnA_GC Cuts at: 2219 2219 Size: 6364 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4489 5354 5652 4489 Size: 865 298 5201 Fragments arranged by size: 5201 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2986 5027 882 Size: 617 1487 2041 2219 Fragments arranged by size: 2219 2041 1487 617 BsaAI yAC'GTr Cuts at: 493 1204 2752 3661 4155 493 Size: 711 1548 909 494 2702 Fragments arranged by size: 2702 1548 909 711 494 BsaBI GATnn'nnATC Cuts at: 6358 6358 Size: 6364 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2079 3548 4171 4553 Size: 53 83 186 936 546 1469 623 382 Cuts at: 4553 5188 275 Size: 635 1451 Fragments arranged by size: 1469 1451 936 635 623 546 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2820 4797 5195 5469 5616 6027 2820 Size: 1977 398 274 147 411 3157 Fragments arranged by size: 3157 1977 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2198 2228 1178 Size: 30 165 30 795 30 5314 Fragments arranged by size: 5314 795 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6364 BseYI C'CCAG_C Cuts at: 2074 2768 2904 5567 6063 2074 Size: 694 136 2663 496 2375 Fragments arranged by size: 2663 2375 694 496 136 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3107 3563 1163 Size: 573 1371 456 3964 Fragments arranged by size: 3964 1371 573 456 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3814 4575 5603 1540 Size: 275 186 1813 761 1028 2301 Fragments arranged by size: 2301 1813 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4425 4510 5581 4425 Size: 85 1071 5208 Fragments arranged by size: 5208 1071 85 BsmI GAATG_Cn' Cuts at: 2669 3309 3875 3968 2669 Size: 640 566 93 5065 Fragments arranged by size: 5065 640 566 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3026 3026 Size: 6364 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2190 3683 4425 4510 5581 1391 Size: 399 387 13 1493 742 85 1071 2174 Fragments arranged by size: 2174 1493 1071 742 399 387 85 13 BspEI T'CCGG_A Cuts at: 2820 6027 2820 Size: 3207 3157 BspHI T'CATG_A Cuts at: 3357 4253 5983 3357 Size: 896 1730 3738 Fragments arranged by size: 3738 1730 896 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2098 3719 844 Size: 1254 1621 3489 Fragments arranged by size: 3489 1621 1254 BsrBI CCG'CTC Cuts at: 1394 1823 3198 4251 1394 Size: 429 1375 1053 3507 Fragments arranged by size: 3507 1375 1053 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4859 5033 65 Size: 1183 3611 174 1396 Fragments arranged by size: 3611 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5008 5195 1700 Size: 110 3198 187 2869 Fragments arranged by size: 3198 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6364 BssHII G'CGCG_C Cuts at: 1925 2103 2123 1925 Size: 178 20 6166 Fragments arranged by size: 6166 178 20 BssSI C'ACGA_G Cuts at: 3125 4424 5436 3125 Size: 1299 1012 4053 Fragments arranged by size: 4053 1299 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6364 BstBI TT'CG_AA Cuts at: 2007 5168 2007 Size: 3161 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6364 BstYI r'GATC_y Cuts at: 1972 3387 3694 4061 4446 4463 5915 6001 6013 Size: 1415 307 367 385 17 1452 86 12 Cuts at: 6013 6359 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1415 385 367 346 307 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2096 3253 513 Size: 552 957 74 1157 3624 Fragments arranged by size: 3624 1157 957 552 74 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2743 3031 487 Size: 683 56 1517 288 3820 Fragments arranged by size: 3820 1517 683 288 56 BtsI GCAGTG_nn' Cuts at: 3119 3880 4674 4694 3119 Size: 761 794 20 4789 Fragments arranged by size: 4789 794 761 20 ClaI AT'CG_AT Cuts at: 1554 2877 3165 1554 Size: 1323 288 4753 Fragments arranged by size: 4753 1323 288 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6329 DraI TTT'AAA Cuts at: 3439 3668 3439 Size: 229 6135 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2978 3353 5371 809 Size: 507 1662 375 2018 1802 Fragments arranged by size: 2018 1802 1662 507 375 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2442 2754 3811 Size: 54 1425 50 275 18 612 312 1057 Cuts at: 3811 4700 6329 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1057 889 612 312 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3811 1537 Size: 275 1999 4090 Fragments arranged by size: 4090 1999 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2182 2915 3287 3402 4294 1353 Size: 829 733 372 115 892 3423 Fragments arranged by size: 3423 892 829 733 372 115 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4951 5325 5471 387 Size: 1064 138 3362 374 146 1280 Fragments arranged by size: 3362 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2464 2813 3203 4427 5024 5811 6079 Size: 393 832 349 390 1224 597 787 268 Cuts at: 6079 1239 Size: 1524 Fragments arranged by size: 1524 1224 832 787 597 393 390 349 268 EcoO109I rG'GnC_Cy Cuts at: 3205 3205 Size: 6364 EcoRI G'AATT_C Cuts at: 2299 3673 4051 2299 Size: 1374 378 4612 Fragments arranged by size: 4612 1374 378 EcoRV GAT'ATC Cuts at: 3163 3163 Size: 6364 FspI TGC'GCA Cuts at: 4870 4870 Size: 6364 HaeII r_GCGC'y Cuts at: 1678 2030 2054 2082 3551 5511 1678 Size: 352 24 28 1469 1960 2531 Fragments arranged by size: 2531 1960 1469 352 28 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 2477 2509 3158 Size: 32 135 32 626 32 247 32 649 Cuts at: 3158 3190 3379 3411 5005 5037 5079 5111 1373 Size: 32 189 32 1594 32 42 32 2626 Fragments arranged by size: 2626 1594 649 626 247 189 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3646 3708 3954 669 Size: 988 281 1708 62 246 3079 Fragments arranged by size: 3079 1708 988 281 246 62 HindIII A'AGCT_T Cuts at: 748 748 Size: 6364 HpaI GTT'AAC Cuts at: 3954 3954 Size: 6364 KasI G'GCGC_C Cuts at: 2078 3547 2078 Size: 1469 4895 KpnI G_GTAC'C Cuts at: 3689 3689 Size: 6364 MfeI C'AATT_G Cuts at: 3963 3963 Size: 6364 MluI A'CGCG_T Cuts at: 6240 6240 Size: 6364 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2927 5478 5662 1305 Size: 1622 2551 184 2007 Fragments arranged by size: 2551 2007 1622 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2444 2756 10 Size: 54 1425 343 612 312 3618 Fragments arranged by size: 3618 1425 612 343 312 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2810 4322 4681 4840 518 Size: 648 1002 642 1512 359 159 2042 Fragments arranged by size: 2042 1512 1002 648 642 359 159 MspA1I CmG'CkG Cuts at: 1606 2098 2145 2287 3566 3780 4457 5605 5850 Size: 492 47 142 1279 214 677 1148 245 Cuts at: 5850 1606 Size: 2120 Fragments arranged by size: 2120 1279 1148 677 492 245 214 142 47 NarI GG'CG_CC Cuts at: 2079 3548 2079 Size: 1469 4895 NcoI C'CATG_G Cuts at: 513 1065 2022 3253 513 Size: 552 957 1231 3624 Fragments arranged by size: 3624 1231 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6364 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6364 NotI GC'GGCC_GC Cuts at: 3811 3811 Size: 6364 NspI r_CATG'y Cuts at: 1132 1229 3722 5267 1132 Size: 97 2493 1545 2229 Fragments arranged by size: 2493 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3460 1348 Size: 2112 4252 PciI A'CATG_T Cuts at: 5263 5263 Size: 6364 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6364 PfoI T'CCnGG_A Cuts at: 2322 2322 Size: 6364 PmeI GTTT'AAAC Cuts at: 3668 3668 Size: 6364 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4433 4465 5946 5978 4433 Size: 32 1481 32 4819 Fragments arranged by size: 4819 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3205 3205 Size: 6364 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6364 PsiI TTA'TAA Cuts at: 3084 3934 3084 Size: 850 5514 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2732 2764 2732 Size: 32 6332 PstI C_TGCA'G Cuts at: 830 2286 3426 3716 830 Size: 1456 1140 290 3478 Fragments arranged by size: 3478 1456 1140 290 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6364 PvuII CAG'CTG Cuts at: 2145 2287 3566 2145 Size: 142 1279 4943 Fragments arranged by size: 4943 1279 142 SacII CC_GC'GG Cuts at: 2099 2099 Size: 6364 SalI G'TCGA_C Cuts at: 1936 3706 1936 Size: 1770 4594 SapI GCTCTTCn'nnn_ Cuts at: 2182 2915 3287 3402 2182 Size: 733 372 115 5144 Fragments arranged by size: 5144 733 372 115 SbfI CC_TGCA'GG Cuts at: 3716 3716 Size: 6364 ScaI AGT'ACT Cuts at: 4612 6324 4612 Size: 1712 4652 SfcI C'TryA_G Cuts at: 826 1045 2282 2673 3422 3712 4847 5528 5719 Size: 219 1237 391 749 290 1135 681 191 Cuts at: 5719 826 Size: 1471 Fragments arranged by size: 1471 1237 1135 749 681 391 290 219 191 SfoI GGC'GCC Cuts at: 2080 3549 2080 Size: 1469 4895 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6364 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6364 SmaI CCC'GGG Cuts at: 1872 3353 3407 3691 1872 Size: 1481 54 284 4545 Fragments arranged by size: 4545 1481 284 54 SmlI C'TyrA_G Cuts at: 820 1017 1943 3649 4468 5369 5631 820 Size: 197 926 1706 819 901 262 1553 Fragments arranged by size: 1706 1553 926 901 819 262 197 SnaBI TAC'GTA Cuts at: 493 3661 4155 493 Size: 3168 494 2702 Fragments arranged by size: 3168 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6364 SphI G_CATG'C Cuts at: 1132 3722 1132 Size: 2590 3774 SspI AAT'ATT Cuts at: 5 52 4288 5 Size: 47 4236 2081 Fragments arranged by size: 4236 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6364 StyI C'CwwG_G Cuts at: 513 1065 2022 2151 2468 3253 3803 513 Size: 552 957 129 317 785 550 3074 Fragments arranged by size: 3074 957 785 552 550 317 129 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2173 2271 4391 4408 4561 4746 5191 Size: 1255 593 98 2120 17 153 185 445 Cuts at: 5191 325 Size: 1498 Fragments arranged by size: 2120 1498 1255 593 445 185 153 98 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4610 6322 96 Size: 274 80 33 51 1443 2633 1712 138 Fragments arranged by size: 2633 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6324 6356 6324 Size: 32 6332 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6364 XbaI T'CTAG_A Cuts at: 3700 3700 Size: 6364 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2912 2912 Size: 6364 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6364 XmaI C'CCGG_G Cuts at: 1870 3351 3405 3689 1870 Size: 1481 54 284 4545 Fragments arranged by size: 4545 1481 284 54 XmnI GAAnn'nnTTC Cuts at: 2263 3296 4493 2263 Size: 1033 1197 4134 Fragments arranged by size: 4134 1197 1033 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4172 276 Size: 53 83 186 936 2638 2468 Fragments arranged by size: 2638 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbvCI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI RsrII SacI SanDI SexAI SfiI SrfI SwaI