Restriction map for
pFN21ASDA1916
(Linear) (Six-base) MAPSORT of: KIAA1916.seq Check: 5497 from: 1 to: 1635
KIAA1916.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 75 1635
Size: 75 1560
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 1635
Size: 441 1194
AfeI AGC'GCT
Cuts at: 0 29 1635
Size: 29 1606
AflII C'TTAA_G
Cuts at: 0 1626 1635
Size: 1626 9
AlwNI CAG_nnn'CTG
Cuts at: 0 1098 1635
Size: 1098 537
ApoI r'AATT_y
Cuts at: 0 276 502 555 792 931 940 1635
Size: 276 226 53 237 139 9 695
Fragments arranged by size:
695 276 237 226 139 53 9
AvaI C'yCGr_G
Cuts at: 0 31 1328 1382 1635
Size: 31 1297 54 253
Fragments arranged by size:
1297 253 54 31
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 580 613 1635
Size: 580 33 1022
Fragments arranged by size:
1022 580 33
BanI G'GyrC_C
Cuts at: 0 55 162 1524 1635
Size: 55 107 1362 111
Fragments arranged by size:
1362 111 107 55
BanII G_rGCy'C
Cuts at: 0 154 1635
Size: 154 1481
BbeI G_GCGC'C
Cuts at: 0 59 1528 1635
Size: 59 1469 107
Fragments arranged by size:
1469 107 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 447 1635
Size: 447 1188
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1510 1544 1635
Size: 1510 34 91
Fragments arranged by size:
1510 91 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 693 1635
Size: 693 942
BclI T'GATC_A
Cuts at: 0 858 1635
Size: 858 777
Bme1580I G_kGCm'C
Cuts at: 0 167 1635
Size: 167 1468
BmgBI CAC'GTC
Cuts at: 0 621 1635
Size: 621 1014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 790 1180 1635
Size: 790 390 455
Fragments arranged by size:
790 455 390
Bpu10I CC'TnA_GC
Cuts at: 0 196 1635
Size: 196 1439
BsaI GGTCTCn'nnnn_
Cuts at: 0 963 1635
Size: 963 672
BsaAI yAC'GTr
Cuts at: 0 729 1635
Size: 729 906
BsaHI Gr'CG_yC
Cuts at: 0 56 1525 1635
Size: 56 1469 110
Fragments arranged by size:
1469 110 56
BsaWI w'CCGG_w
Cuts at: 0 797 1635
Size: 797 838
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 175 205 1635
Size: 175 30 1430
Fragments arranged by size:
1430 175 30
BseYI C'CCAG_C
Cuts at: 0 51 745 881 1635
Size: 51 694 136 754
Fragments arranged by size:
754 694 136 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1084 1540 1635
Size: 1084 456 95
Fragments arranged by size:
1084 456 95
BsmI GAATG_Cn'
Cuts at: 0 646 1286 1635
Size: 646 640 349
Fragments arranged by size:
646 640 349
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1003 1635
Size: 1003 632
Bsp1286I G_dGCh'C
Cuts at: 0 154 167 1635
Size: 154 13 1468
Fragments arranged by size:
1468 154 13
BspEI T'CCGG_A
Cuts at: 0 797 1635
Size: 797 838
BspHI T'CATG_A
Cuts at: 0 1334 1635
Size: 1334 301
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 75 1635
Size: 75 1560
BsrBI CCG'CTC
Cuts at: 0 1175 1635
Size: 1175 460
BssHII G'CGCG_C
Cuts at: 0 80 100 1635
Size: 80 20 1535
Fragments arranged by size:
1535 80 20
BssSI C'ACGA_G
Cuts at: 0 1102 1635
Size: 1102 533
BstYI r'GATC_y
Cuts at: 0 1364 1635
Size: 1364 271
BtgI C'CryG_G
Cuts at: 0 73 1230 1635
Size: 73 1157 405
Fragments arranged by size:
1157 405 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 720 1008 1635
Size: 720 288 627
Fragments arranged by size:
720 627 288
BtsI GCAGTG_nn'
Cuts at: 0 1096 1635
Size: 1096 539
ClaI AT'CG_AT
Cuts at: 0 854 1142 1635
Size: 854 288 493
Fragments arranged by size:
854 493 288
DraI TTT'AAA
Cuts at: 0 1416 1635
Size: 1416 219
DrdI GACnn_nn'nnGTC
Cuts at: 0 955 1330 1635
Size: 955 375 305
Fragments arranged by size:
955 375 305
EaeI y'GGCC_r
Cuts at: 0 419 731 1635
Size: 419 312 904
Fragments arranged by size:
904 419 312
EarI CTCTTCn'nnn_
Cuts at: 0 159 892 1264 1379 1635
Size: 159 733 372 115 256
Fragments arranged by size:
733 372 256 159 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 441 790 1180 1635
Size: 441 349 390 455
Fragments arranged by size:
455 441 390 349
EcoO109I rG'GnC_Cy
Cuts at: 0 1182 1635
Size: 1182 453
EcoRI G'AATT_C
Cuts at: 0 276 1635
Size: 276 1359
EcoRV GAT'ATC
Cuts at: 0 1140 1635
Size: 1140 495
HaeII r_GCGC'y
Cuts at: 0 7 31 59 1528 1635
Size: 7 24 28 1469 107
Fragments arranged by size:
1469 107 28 24 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 454 486 1135 1167 1356 1388
Size: 175 32 247 32 649 32 189 32
Cuts at: 1388 1635
Size: 247
Fragments arranged by size:
649 247 247 189 175 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1623 1635
Size: 1623 12
KasI G'GCGC_C
Cuts at: 0 55 1524 1635
Size: 55 1469 111
Fragments arranged by size:
1469 111 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 904 1635
Size: 904 731
MscI TGG'CCA
Cuts at: 0 421 733 1635
Size: 421 312 902
Fragments arranged by size:
902 421 312
MslI CAynn'nnrTG
Cuts at: 0 145 787 1635
Size: 145 642 848
Fragments arranged by size:
848 642 145
MspA1I CmG'CkG
Cuts at: 0 75 122 264 1543 1635
Size: 75 47 142 1279 92
Fragments arranged by size:
1279 142 92 75 47
NarI GG'CG_CC
Cuts at: 0 56 1525 1635
Size: 56 1469 110
Fragments arranged by size:
1469 110 56
NcoI C'CATG_G
Cuts at: 0 1230 1635
Size: 1230 405
PasI CC'CwG_GG
Cuts at: 0 1437 1635
Size: 1437 198
PfoI T'CCnGG_A
Cuts at: 0 299 1635
Size: 299 1336
PpuMI rG'GwC_Cy
Cuts at: 0 1182 1635
Size: 1182 453
PsiI TTA'TAA
Cuts at: 0 1061 1635
Size: 1061 574
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 709 741 1635
Size: 709 32 894
Fragments arranged by size:
894 709 32
PstI C_TGCA'G
Cuts at: 0 263 1403 1635
Size: 263 1140 232
Fragments arranged by size:
1140 263 232
PvuII CAG'CTG
Cuts at: 0 122 264 1543 1635
Size: 122 142 1279 92
Fragments arranged by size:
1279 142 122 92
SacII CC_GC'GG
Cuts at: 0 76 1635
Size: 76 1559
SapI GCTCTTCn'nnn_
Cuts at: 0 159 892 1264 1379 1635
Size: 159 733 372 115 256
Fragments arranged by size:
733 372 256 159 115
SfcI C'TryA_G
Cuts at: 0 259 650 1399 1635
Size: 259 391 749 236
Fragments arranged by size:
749 391 259 236
SfoI GGC'GCC
Cuts at: 0 57 1526 1635
Size: 57 1469 109
Fragments arranged by size:
1469 109 57
SmaI CCC'GGG
Cuts at: 0 1330 1384 1635
Size: 1330 54 251
Fragments arranged by size:
1330 251 54
SmlI C'TyrA_G
Cuts at: 0 1626 1635
Size: 1626 9
StyI C'CwwG_G
Cuts at: 0 128 445 1230 1635
Size: 128 317 785 405
Fragments arranged by size:
785 405 317 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 150 248 1635
Size: 150 98 1387
Fragments arranged by size:
1387 150 98
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 889 1635
Size: 889 746
XmaI C'CCGG_G
Cuts at: 0 1328 1382 1635
Size: 1328 54 253
Fragments arranged by size:
1328 253 54
XmnI GAAnn'nnTTC
Cuts at: 0 240 1273 1635
Size: 240 1033 362
Fragments arranged by size:
1033 362 240
Enzymes that do cut:
AarI AcuI AfeI AflII AlwNI ApoI AvaI BaeI
BanI BanII BbeI BbsI BcgI BciVI BclI Bme1580I
BmgBI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI
BseYI BsgI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BssHII BssSI BstYI BtgI BtgZI BtsI ClaI
DraI DrdI EaeI EarI Eco57MI EcoO109I EcoRI EcoRV
HaeII Hin4I HincII KasI MmeI MscI MslI MspA1I
NarI NcoI PasI PfoI PpuMI PsiI PsrI PstI
PvuII SacII SapI SfcI SfoI SmaI SmlI StyI
TaqII XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BamHI BbvCI BfrBI BglI BglII BlpI BmrI BmtI
BplI BpuEI BsaBI BseRI BsiEI BsiHKAI BsiWI BsrDI
BsrFI BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
CspCI DraIII EagI EciI EcoICRI EcoNI FalI FseI
FspI FspAI HindIII HpaI KpnI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PciI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI
PvuI RsrII SacI SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI TstI Tth111I XbaI XhoI ZraI