(Circular) (Six-base) MAPSORT of: pFN21ASDA1912.seq Check: 8954 from: 1 to: 6388 pFN21ASDA1912 6388 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2739 2739 Size: 6388 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4198 278 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3246 3731 1937 Size: 1309 485 4594 Fragments arranged by size: 4594 1309 485 Acc65I G'GTAC_C Cuts at: 3709 3709 Size: 6388 AclI AA'CG_TT Cuts at: 1563 4515 4888 1563 Size: 2952 373 3063 Fragments arranged by size: 3063 2952 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3023 3523 4451 5835 6103 1632 Size: 1391 500 928 1384 268 1917 Fragments arranged by size: 1917 1391 1384 928 500 268 AflII C'TTAA_G Cuts at: 820 1017 3590 820 Size: 197 2573 3618 Fragments arranged by size: 3618 2573 197 AflIII A'CryG_T Cuts at: 1203 5287 6264 1203 Size: 4084 977 1327 Fragments arranged by size: 4084 1327 977 AgeI A'CCGG_T Cuts at: 2982 5219 2982 Size: 2237 4151 AhdI GACnn_n'nnGTC Cuts at: 5117 5117 Size: 6388 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6388 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6388 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3499 3531 3499 Size: 32 6356 AlwNI CAG_nnn'CTG Cuts at: 2335 2407 2464 2574 2814 3500 4131 5703 2335 Size: 72 57 110 240 686 631 1572 3020 Fragments arranged by size: 3020 1572 686 631 240 110 72 57 ApaLI G'TGCA_C Cuts at: 4445 5601 4445 Size: 1156 5232 ApoI r'AATT_y Cuts at: 1493 3697 3923 4075 6184 1493 Size: 2204 226 152 2109 1697 Fragments arranged by size: 2204 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2194 2194 Size: 6388 AseI AT'TA_AT Cuts at: 160 1032 4942 160 Size: 872 3910 1606 Fragments arranged by size: 3910 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 AvaI C'yCGr_G Cuts at: 1870 1943 2391 2789 3472 3713 1870 Size: 73 448 398 683 241 4545 Fragments arranged by size: 4545 683 448 398 241 73 BamHI G'GATC_C Cuts at: 3718 4085 3718 Size: 367 6021 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2904 3089 3194 3438 3709 Size: 325 217 627 1117 185 105 244 271 Cuts at: 3709 5164 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1117 627 325 271 244 217 185 105 BanII G_rGCy'C Cuts at: 1391 2517 2526 2963 3029 3131 3412 3707 1391 Size: 1126 9 437 66 102 281 295 4072 Fragments arranged by size: 4072 1126 437 295 281 102 66 9 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2328 928 Size: 971 429 4988 Fragments arranged by size: 4988 971 429 BbvCI CC'TCA_GC Cuts at: 2080 2080 Size: 6388 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4579 4613 1903 Size: 34 2642 34 3678 Fragments arranged by size: 3678 2642 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4280 5490 4280 Size: 1210 5178 BclI T'GATC_A Cuts at: 1548 3133 1548 Size: 1585 4803 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4999 136 Size: 107 122 71 631 605 3327 1525 Fragments arranged by size: 3327 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6383 6383 Size: 6388 BlpI GC'TnA_GC Cuts at: 3805 3805 Size: 6388 Bme1580I G_kGCm'C Cuts at: 1790 3092 3197 4449 5605 1790 Size: 1302 105 1252 1156 2573 Fragments arranged by size: 2573 1302 1252 1156 105 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6388 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2417 4119 5077 Size: 416 53 83 399 24 1000 1702 958 Cuts at: 5077 442 Size: 1753 Fragments arranged by size: 1753 1702 1000 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3537 1056 Size: 2481 3907 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2406 2406 Size: 6388 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2495 2647 3311 3332 5048 1239 Size: 1061 195 152 664 21 1716 2579 Fragments arranged by size: 2579 1716 1061 664 195 152 21 Bpu10I CC'TnA_GC Cuts at: 2080 2080 Size: 6388 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2279 2501 4513 5378 5676 2279 Size: 222 2012 865 298 2991 Fragments arranged by size: 2991 2012 865 298 222 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5051 882 Size: 617 3552 2219 Fragments arranged by size: 3552 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2632 2947 3685 4179 493 Size: 711 1428 315 738 494 2702 Fragments arranged by size: 2702 1428 738 711 494 315 BsaBI GATnn'nnATC Cuts at: 6382 6382 Size: 6388 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4195 4577 5212 275 Size: 53 83 186 936 2662 382 635 1451 Fragments arranged by size: 2662 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2982 3237 4821 5219 5493 5640 6051 2982 Size: 255 1584 398 274 147 411 3319 Fragments arranged by size: 3319 1584 411 398 274 255 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2114 2144 3209 3239 3499 Size: 30 165 30 711 30 1065 30 260 Cuts at: 3499 3529 1178 Size: 30 4037 Fragments arranged by size: 4037 1065 711 260 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 2186 2558 3162 1186 Size: 976 24 372 604 4412 Fragments arranged by size: 4412 976 604 372 24 BseYI C'CCAG_C Cuts at: 3396 3561 5591 6087 3396 Size: 165 2030 496 3697 Fragments arranged by size: 3697 2030 496 165 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2255 2574 1163 Size: 573 519 319 4977 Fragments arranged by size: 4977 573 519 319 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2037 2091 3838 4599 5627 1540 Size: 275 186 36 54 1747 761 1028 2301 Fragments arranged by size: 2301 1747 1028 761 275 186 54 36 BsiHKAI G_wGCw'C Cuts at: 2517 2526 2730 2766 2865 3105 4449 4534 5605 Size: 9 204 36 99 240 1344 85 1071 Cuts at: 5605 2517 Size: 3300 Fragments arranged by size: 3300 1344 1071 240 204 99 85 36 9 BsmI GAATG_Cn' Cuts at: 3899 3992 3899 Size: 93 6295 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2517 2526 2730 2766 2865 2963 3029 Size: 399 727 9 204 36 99 98 66 Cuts at: 3029 3092 3105 3131 3197 3412 3707 4449 4534 Size: 63 13 26 66 215 295 742 85 Cuts at: 4534 5605 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 727 399 295 215 204 99 98 85 66 66 63 36 26 13 9 BspEI T'CCGG_A Cuts at: 6051 6051 Size: 6388 BspHI T'CATG_A Cuts at: 4277 6007 4277 Size: 1730 4658 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2463 2739 3614 3743 844 Size: 1619 276 875 129 3489 Fragments arranged by size: 3489 1619 875 276 129 BsrBI CCG'CTC Cuts at: 1394 1823 4275 1394 Size: 429 2452 3507 Fragments arranged by size: 3507 2452 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4883 5057 65 Size: 1183 3635 174 1396 Fragments arranged by size: 3635 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2090 2116 2367 2982 5032 5219 1700 Size: 110 280 26 251 615 2050 187 2869 Fragments arranged by size: 2869 2050 615 280 251 187 110 26 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6388 BssHII G'CGCG_C Cuts at: 1925 2194 1925 Size: 269 6119 BssSI C'ACGA_G Cuts at: 4448 5460 4448 Size: 1012 5376 BstAPI GCAn_nnn'nTGC Cuts at: 794 2574 794 Size: 1780 4608 BstBI TT'CG_AA Cuts at: 2007 5192 2007 Size: 3185 3203 BstEII G'GTnAC_C Cuts at: 1184 3225 1184 Size: 2041 4347 BstYI r'GATC_y Cuts at: 1972 2283 3718 4085 4470 4487 5939 6025 6037 Size: 311 1435 367 385 17 1452 86 12 Cuts at: 6037 6383 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1435 385 367 346 311 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2100 2287 513 Size: 552 1035 187 4614 Fragments arranged by size: 4614 1035 552 187 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2234 487 Size: 683 56 1008 4641 Fragments arranged by size: 4641 1008 683 56 BtsI GCAGTG_nn' Cuts at: 2165 2418 2719 3160 3431 3904 4698 4718 2165 Size: 253 301 441 271 473 794 20 3835 Fragments arranged by size: 3835 794 473 441 301 271 253 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6388 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6353 DraI TTT'AAA Cuts at: 3692 3692 Size: 6388 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5395 809 Size: 507 4079 1802 Fragments arranged by size: 4079 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 2088 3835 Size: 54 1425 50 275 18 204 54 1747 Cuts at: 3835 4724 6353 8 Size: 889 1629 43 Fragments arranged by size: 1747 1629 1425 889 275 204 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2034 2088 3835 1537 Size: 275 222 54 1747 4090 Fragments arranged by size: 4090 1747 275 222 54 EarI CTCTTCn'nnn_ Cuts at: 1353 4318 1353 Size: 2965 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2081 2219 3192 4975 5349 5495 Size: 1064 138 492 138 973 1783 374 146 Cuts at: 5495 387 Size: 1280 Fragments arranged by size: 1783 1280 1064 973 492 374 146 138 138 EcoICRI GAG'CTC Cuts at: 2515 2524 2515 Size: 9 6379 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2495 2647 3023 3311 3332 3523 Size: 393 668 195 152 376 288 21 191 Cuts at: 3523 4451 5048 5835 6103 1239 Size: 928 597 787 268 1524 Fragments arranged by size: 1524 928 787 668 597 393 376 288 268 195 191 152 21 EcoNI CCTnn'n_nnAGG Cuts at: 3280 3280 Size: 6388 EcoO109I rG'GnC_Cy Cuts at: 2125 2341 2849 2125 Size: 216 508 5664 Fragments arranged by size: 5664 508 216 EcoRI G'AATT_C Cuts at: 3697 4075 3697 Size: 378 6010 EcoRV GAT'ATC Cuts at: 2302 2302 Size: 6388 FspI TGC'GCA Cuts at: 2563 4894 2563 Size: 2331 4057 HaeII r_GCGC'y Cuts at: 1678 2784 5535 1678 Size: 1106 2751 2531 Fragments arranged by size: 2751 2531 1106 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2114 2146 2395 2427 3118 Size: 32 135 32 542 32 249 32 691 Cuts at: 3118 3150 3209 3241 5029 5061 5103 5135 1373 Size: 32 59 32 1788 32 42 32 2626 Fragments arranged by size: 2626 1788 691 542 249 135 59 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3732 3978 669 Size: 988 281 1794 246 3079 Fragments arranged by size: 3079 1794 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6388 HpaI GTT'AAC Cuts at: 3978 3978 Size: 6388 KpnI G_GTAC'C Cuts at: 3713 3713 Size: 6388 MfeI C'AATT_G Cuts at: 3987 3987 Size: 6388 MluI A'CGCG_T Cuts at: 6264 6264 Size: 6388 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2121 3389 5502 5686 1305 Size: 816 1268 2113 184 2007 Fragments arranged by size: 2113 2007 1268 816 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4566 Fragments arranged by size: 4566 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3240 4346 4705 4864 518 Size: 648 2074 1106 359 159 2042 Fragments arranged by size: 2074 2042 1106 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2102 2269 2289 2574 3174 3289 3310 3804 Size: 496 167 20 285 600 115 21 494 Cuts at: 3804 4481 5629 5874 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 600 496 494 285 245 167 115 21 20 NaeI GCC'GGC Cuts at: 2092 2092 Size: 6388 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5836 NdeI CA'TA_TG Cuts at: 387 387 Size: 6388 NgoMIV G'CCGG_C Cuts at: 2090 2090 Size: 6388 NheI G'CTAG_C Cuts at: 1052 3533 1052 Size: 2481 3907 NotI GC'GGCC_GC Cuts at: 3835 3835 Size: 6388 NspI r_CATG'y Cuts at: 1132 1229 3331 3551 3746 5291 1132 Size: 97 2102 220 195 1545 2229 Fragments arranged by size: 2229 2102 1545 220 195 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6388 PciI A'CATG_T Cuts at: 5287 5287 Size: 6388 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6388 PfoI T'CCnGG_A Cuts at: 2316 2355 3634 2316 Size: 39 1279 5070 Fragments arranged by size: 5070 1279 39 PmeI GTTT'AAAC Cuts at: 3692 3692 Size: 6388 PmlI CAC'GTG Cuts at: 2947 2947 Size: 6388 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3499 3531 4457 4489 5970 6002 3499 Size: 32 926 32 1481 32 3885 Fragments arranged by size: 3885 1481 926 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2125 2341 2849 2125 Size: 216 508 5664 Fragments arranged by size: 5664 508 216 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6388 PsiI TTA'TAA Cuts at: 3958 3958 Size: 6388 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3564 3596 3564 Size: 32 6356 PstI C_TGCA'G Cuts at: 830 2160 2268 2460 2736 3532 3625 3740 830 Size: 1330 108 192 276 796 93 115 3478 Fragments arranged by size: 3478 1330 796 276 192 115 108 93 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6388 PvuII CAG'CTG Cuts at: 2269 3289 3310 2269 Size: 1020 21 5347 Fragments arranged by size: 5347 1020 21 SacI G_AGCT'C Cuts at: 2517 2526 2517 Size: 9 6379 SacII CC_GC'GG Cuts at: 2103 2290 2103 Size: 187 6201 SalI G'TCGA_C Cuts at: 1936 3730 1936 Size: 1794 4594 SbfI CC_TGCA'GG Cuts at: 3740 3740 Size: 6388 ScaI AGT'ACT Cuts at: 4636 6348 4636 Size: 1712 4676 SfcI C'TryA_G Cuts at: 826 1045 2156 2264 2456 2732 3247 3528 3621 Size: 219 1111 108 192 276 515 281 93 Cuts at: 3621 3736 4871 5552 5743 826 Size: 115 1135 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 515 281 276 219 192 191 115 108 93 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6388 SgrAI Cr'CCGG_yG Cuts at: 1700 2116 1700 Size: 416 5972 SmaI CCC'GGG Cuts at: 1872 3474 3715 1872 Size: 1602 241 4545 Fragments arranged by size: 4545 1602 241 SmlI C'TyrA_G Cuts at: 820 1017 1943 2294 2516 3590 4492 5393 5655 Size: 197 926 351 222 1074 902 901 262 Cuts at: 5655 820 Size: 1553 Fragments arranged by size: 1553 1074 926 902 901 351 262 222 197 SnaBI TAC'GTA Cuts at: 493 3685 4179 493 Size: 3192 494 2702 Fragments arranged by size: 3192 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6388 SphI G_CATG'C Cuts at: 1132 3331 3746 1132 Size: 2199 415 3774 Fragments arranged by size: 3774 2199 415 SspI AAT'ATT Cuts at: 5 52 4312 5 Size: 47 4260 2081 Fragments arranged by size: 4260 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6388 StyI C'CwwG_G Cuts at: 513 1065 3827 513 Size: 552 2762 3074 Fragments arranged by size: 3074 2762 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2633 3535 4415 4432 4585 4770 5215 Size: 1255 1053 902 880 17 153 185 445 Cuts at: 5215 325 Size: 1498 Fragments arranged by size: 1498 1255 1053 902 880 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3666 4634 6346 Size: 274 80 33 51 1443 1689 968 1712 Cuts at: 6346 96 Size: 138 Fragments arranged by size: 1712 1689 1443 968 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6348 6380 6348 Size: 32 6356 Tth111I GACn'n_nGTC Cuts at: 1316 2409 1316 Size: 1093 5295 XbaI T'CTAG_A Cuts at: 3724 3724 Size: 6388 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2711 2711 Size: 6388 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6388 XmaI C'CCGG_G Cuts at: 1870 3472 3713 1870 Size: 1602 241 4545 Fragments arranged by size: 4545 1602 241 XmnI GAAnn'nnTTC Cuts at: 4517 4517 Size: 6388 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4196 276 Size: 53 83 186 936 2662 2468 Fragments arranged by size: 2662 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI RsrII SanDI SapI SexAI SfiI SfoI SrfI SwaI