(Circular) (Six-base) MAPSORT of: pFN21ASDA1895.seq Check: 7361 from: 1 to: 6562 pFN21ASDA1895 6562 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3143 3143 Size: 6562 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4372 278 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3566 3905 1937 Size: 1629 339 4594 Fragments arranged by size: 4594 1629 339 Acc65I G'GTAC_C Cuts at: 3883 3883 Size: 6562 AclI AA'CG_TT Cuts at: 1563 3020 4689 5062 1563 Size: 1457 1669 373 3063 Fragments arranged by size: 3063 1669 1457 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2487 3377 4625 6009 6277 1632 Size: 855 890 1248 1384 268 1917 Fragments arranged by size: 1917 1384 1248 890 855 268 AflII C'TTAA_G Cuts at: 820 1017 2303 820 Size: 197 1286 5079 Fragments arranged by size: 5079 1286 197 AflIII A'CryG_T Cuts at: 1203 3520 5461 6438 1203 Size: 2317 1941 977 1327 Fragments arranged by size: 2317 1941 1327 977 AgeI A'CCGG_T Cuts at: 5393 5393 Size: 6562 AhdI GACnn_n'nnGTC Cuts at: 5291 5291 Size: 6562 AleI CACnn'nnGTG Cuts at: 1166 2891 1166 Size: 1725 4837 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3486 1184 Size: 2302 4260 AlwNI CAG_nnn'CTG Cuts at: 4305 5877 4305 Size: 1572 4990 ApaLI G'TGCA_C Cuts at: 4619 5775 4619 Size: 1156 5406 ApoI r'AATT_y Cuts at: 1493 2570 3040 3626 3871 4097 4249 6358 1493 Size: 1077 470 586 245 226 152 2109 1697 Fragments arranged by size: 2109 1697 1077 586 470 245 226 152 AseI AT'TA_AT Cuts at: 160 1032 5116 160 Size: 872 4084 1606 Fragments arranged by size: 4084 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 AvaI C'yCGr_G Cuts at: 1870 1943 3887 1870 Size: 73 1944 4545 Fragments arranged by size: 4545 1944 73 BamHI G'GATC_C Cuts at: 3892 4259 3892 Size: 367 6195 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3883 5338 618 Size: 325 217 627 2096 1455 1842 Fragments arranged by size: 2096 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3881 1391 Size: 2490 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2232 928 Size: 971 333 5258 Fragments arranged by size: 5258 971 333 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4753 4787 1903 Size: 34 2816 34 3678 Fragments arranged by size: 3678 2816 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4454 5664 4454 Size: 1210 5352 BclI T'GATC_A Cuts at: 1548 1548 Size: 6562 BfrBI ATG'CAT Cuts at: 3688 3688 Size: 6562 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5173 136 Size: 107 122 71 631 605 3501 1525 Fragments arranged by size: 3501 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6557 6557 Size: 6562 BlpI GC'TnA_GC Cuts at: 3979 3979 Size: 6562 Bme1580I G_kGCm'C Cuts at: 1790 4623 5779 1790 Size: 2833 1156 2573 Fragments arranged by size: 2833 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6562 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2838 2919 4293 Size: 416 53 83 399 24 1421 81 1374 Cuts at: 4293 5251 442 Size: 958 1753 Fragments arranged by size: 1753 1421 1374 958 416 399 83 81 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6562 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3763 3763 Size: 6562 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2339 2747 5222 1239 Size: 1100 408 2475 2579 Fragments arranged by size: 2579 2475 1100 408 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4687 5552 5850 4687 Size: 865 298 5399 Fragments arranged by size: 5399 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2110 2157 5225 882 Size: 617 611 47 3068 2219 Fragments arranged by size: 3068 2219 617 611 47 BsaAI yAC'GTr Cuts at: 493 1204 3859 4353 493 Size: 711 2655 494 2702 Fragments arranged by size: 2702 2655 711 494 BsaBI GATnn'nnATC Cuts at: 6556 6556 Size: 6562 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4369 4751 5386 275 Size: 53 83 186 936 2836 382 635 1451 Fragments arranged by size: 2836 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2101 4995 5393 5667 5814 6225 2101 Size: 2894 398 274 147 411 2438 Fragments arranged by size: 2894 2438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6337 Fragments arranged by size: 6337 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2175 1186 Size: 989 5573 BseYI C'CCAG_C Cuts at: 2781 5765 6261 2781 Size: 2984 496 3082 Fragments arranged by size: 3082 2984 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2868 1163 Size: 573 1132 4857 Fragments arranged by size: 4857 1132 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4012 4773 5801 1540 Size: 275 186 2011 761 1028 2301 Fragments arranged by size: 2301 2011 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2091 4623 4708 5779 2091 Size: 2532 85 1071 2874 Fragments arranged by size: 2874 2532 1071 85 BsmI GAATG_Cn' Cuts at: 2451 4073 4166 2451 Size: 1622 93 4847 Fragments arranged by size: 4847 1622 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3083 3192 3083 Size: 109 6453 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2091 3881 4623 4708 5779 1391 Size: 399 301 1790 742 85 1071 2174 Fragments arranged by size: 2174 1790 1071 742 399 301 85 BspEI T'CCGG_A Cuts at: 6225 6225 Size: 6562 BspHI T'CATG_A Cuts at: 4451 6181 4451 Size: 1730 4832 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3143 3917 844 Size: 2299 774 3489 Fragments arranged by size: 3489 2299 774 BsrBI CCG'CTC Cuts at: 1394 1823 4449 1394 Size: 429 2626 3507 Fragments arranged by size: 3507 2626 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5057 5231 65 Size: 1183 3809 174 1396 Fragments arranged by size: 3809 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5206 5393 1700 Size: 110 3396 187 2869 Fragments arranged by size: 3396 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6562 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6562 BssSI C'ACGA_G Cuts at: 4622 5634 4622 Size: 1012 5550 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6562 BstBI TT'CG_AA Cuts at: 2007 5366 2007 Size: 3359 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6562 BstXI CCAn_nnnn'nTGG Cuts at: 2534 3219 3449 2534 Size: 685 230 5647 Fragments arranged by size: 5647 685 230 BstYI r'GATC_y Cuts at: 1972 3892 4259 4644 4661 6113 6199 6211 6557 Size: 1920 367 385 17 1452 86 12 346 Cuts at: 6557 1972 Size: 1977 Fragments arranged by size: 1977 1920 1452 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3567 3567 Size: 6562 Bsu36I CC'TnA_GG Cuts at: 3489 3489 Size: 6562 BtgI C'CryG_G Cuts at: 513 1065 3753 3794 513 Size: 552 2688 41 3281 Fragments arranged by size: 3281 2688 552 41 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5823 Fragments arranged by size: 5823 683 56 BtsI GCAGTG_nn' Cuts at: 4078 4872 4892 4078 Size: 794 20 5748 Fragments arranged by size: 5748 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6562 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3121 3156 529 Size: 35 2557 35 3935 Fragments arranged by size: 3935 2557 35 35 DraI TTT'AAA Cuts at: 3148 3866 3148 Size: 718 5844 DraIII CAC_nnn'GTG Cuts at: 2318 3419 2318 Size: 1101 5461 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3344 5569 809 Size: 507 2028 2225 1802 Fragments arranged by size: 2225 2028 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2473 3439 3681 Size: 54 1425 50 275 18 643 966 242 Cuts at: 3681 4009 4898 6527 8 Size: 328 889 1629 43 Fragments arranged by size: 1629 1425 966 889 643 328 275 242 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4009 1537 Size: 275 2197 4090 Fragments arranged by size: 4090 2197 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3261 3324 4492 1353 Size: 1908 63 1168 3423 Fragments arranged by size: 3423 1908 1168 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5149 5523 5669 387 Size: 1064 138 3560 374 146 1280 Fragments arranged by size: 3560 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 2487 2747 3377 4625 5222 6009 Size: 393 707 148 260 630 1248 597 787 Cuts at: 6009 6277 1239 Size: 268 1524 Fragments arranged by size: 1524 1248 787 707 630 597 393 268 260 148 EcoRI G'AATT_C Cuts at: 3871 4249 3871 Size: 378 6184 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2417 2417 Size: 6562 FspI TGC'GCA Cuts at: 5068 5068 Size: 6562 HaeII r_GCGC'y Cuts at: 1678 5709 1678 Size: 4031 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2365 2397 2537 2569 2667 Size: 32 135 32 793 32 140 32 98 Cuts at: 2667 2699 2892 2924 5203 5235 5277 5309 1373 Size: 32 193 32 2279 32 42 32 2626 Fragments arranged by size: 2626 2279 793 193 140 135 98 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3906 4152 669 Size: 988 281 1968 246 3079 Fragments arranged by size: 3079 1968 988 281 246 HindIII A'AGCT_T Cuts at: 748 2630 748 Size: 1882 4680 HpaI GTT'AAC Cuts at: 4152 4152 Size: 6562 KpnI G_GTAC'C Cuts at: 3887 3887 Size: 6562 MfeI C'AATT_G Cuts at: 2586 4161 2586 Size: 1575 4987 MluI A'CGCG_T Cuts at: 6438 6438 Size: 6562 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5676 5860 1305 Size: 4371 184 2007 Fragments arranged by size: 4371 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2475 3441 3683 10 Size: 54 1425 343 643 966 242 2889 Fragments arranged by size: 2889 1425 966 643 343 242 54 MslI CAynn'nnrTG Cuts at: 518 1166 2891 3447 4520 4879 5038 518 Size: 648 1725 556 1073 359 159 2042 Fragments arranged by size: 2042 1725 1073 648 556 359 159 MspA1I CmG'CkG Cuts at: 1606 3978 4655 5803 6048 1606 Size: 2372 677 1148 245 2120 Fragments arranged by size: 2372 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3753 3794 513 Size: 552 2688 41 3281 Fragments arranged by size: 3281 2688 552 41 NdeI CA'TA_TG Cuts at: 387 3448 3561 387 Size: 3061 113 3388 Fragments arranged by size: 3388 3061 113 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6562 NotI GC'GGCC_GC Cuts at: 4009 4009 Size: 6562 NsiI A_TGCA'T Cuts at: 3690 3690 Size: 6562 NspI r_CATG'y Cuts at: 1132 1229 3179 3692 3920 5465 1132 Size: 97 1950 513 228 1545 2229 Fragments arranged by size: 2229 1950 1545 513 228 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6562 PciI A'CATG_T Cuts at: 5461 5461 Size: 6562 PflMI CCAn_nnn'nTGG Cuts at: 1108 3419 1108 Size: 2311 4251 PmeI GTTT'AAAC Cuts at: 3866 3866 Size: 6562 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4631 4663 6144 6176 4631 Size: 32 1481 32 5017 Fragments arranged by size: 5017 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6562 PsiI TTA'TAA Cuts at: 4132 4132 Size: 6562 PstI C_TGCA'G Cuts at: 830 3914 830 Size: 3084 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6562 SalI G'TCGA_C Cuts at: 1936 3904 1936 Size: 1968 4594 SapI GCTCTTCn'nnn_ Cuts at: 3261 3324 3261 Size: 63 6499 SbfI CC_TGCA'GG Cuts at: 3914 3914 Size: 6562 ScaI AGT'ACT Cuts at: 2134 4810 6522 2134 Size: 2676 1712 2174 Fragments arranged by size: 2676 2174 1712 SfcI C'TryA_G Cuts at: 826 1045 3910 5045 5726 5917 826 Size: 219 2865 1135 681 191 1471 Fragments arranged by size: 2865 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6562 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6562 SmaI CCC'GGG Cuts at: 1872 3889 1872 Size: 2017 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2303 4666 5567 5829 820 Size: 197 926 360 2363 901 262 1553 Fragments arranged by size: 2363 1553 926 901 360 262 197 SnaBI TAC'GTA Cuts at: 493 3859 4353 493 Size: 3366 494 2702 Fragments arranged by size: 3366 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6562 SphI G_CATG'C Cuts at: 1132 3692 3920 1132 Size: 2560 228 3774 Fragments arranged by size: 3774 2560 228 SspI AAT'ATT Cuts at: 5 52 4486 5 Size: 47 4434 2081 Fragments arranged by size: 4434 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6562 StyI C'CwwG_G Cuts at: 513 1065 2386 2440 3308 3753 3794 4001 513 Size: 552 1321 54 868 445 41 207 3074 Fragments arranged by size: 3074 1321 868 552 445 207 54 41 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2795 4589 4606 4759 4944 5389 325 Size: 1255 1215 1794 17 153 185 445 1498 Fragments arranged by size: 1794 1498 1255 1215 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2132 2430 4808 Size: 274 80 33 51 1443 155 298 2378 Cuts at: 4808 6520 96 Size: 1712 138 Fragments arranged by size: 2378 1712 1443 298 274 155 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2681 2713 6522 6554 2681 Size: 32 3809 32 2689 Fragments arranged by size: 3809 2689 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6562 XbaI T'CTAG_A Cuts at: 2156 3898 2156 Size: 1742 4820 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6562 XmaI C'CCGG_G Cuts at: 1870 3887 1870 Size: 2017 4545 XmnI GAAnn'nnTTC Cuts at: 3522 4691 3522 Size: 1169 5393 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4370 276 Size: 53 83 186 936 2836 2468 Fragments arranged by size: 2836 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI