Restriction map for
pFN21ASDA1886
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# NO CUT ENZYMES ( 90 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AocI ApoI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BbvCI BcgI BclI BfrI
BglII BlnI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BspDI
BspEI BsrDI BssHII Bst1107I BstBI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI EagI EclXI Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MfeI MluI MroI MunI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacI
SalI SapI ScaI SexAI SfuI
SgrAI SnaBI SpeI SphI SspI
SwaI TfiI XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AflIII 1157 ACATGT A^C[GA][CT]GT
Alw44I 1621 GTGCAC G^TGCAC
Aor51HI 2102 AGCGCT AGC^GCT
ApaLI 1621 GTGCAC G^TGCAC
BamHI 1817 GGATCC G^GATCC
BbsI 1342 GTCTTC GAAGAC(2/6)
BpuAI 1342 GTCTTC GAAGAC(2/6)
BsaBI 2013 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 323 TGTACA T^GTACA
BspHI 1839 TCATGA T^CATGA
BspLU11I 1157 ACATGT A^CATGT
BsrGI 323 TGTACA T^GTACA
BssSI 1420 CACGAG CACGAG(-5/-1)
BstEII 2425 GGTCACC G^GT[ACGT]ACC
BstPI 2425 GGTCACC G^GT[ACGT]ACC
BstYI 1817 GGATCC [GA]^GATC[CT]
DraIII 44 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 1603 CTCTTC CTCTTC(1/4)
Eco47III 2102 AGCGCT AGC^GCT
EcoO65I 2425 GGTCACC G^GT[ACGT]ACC
Ksp632I 1603 CTCTTC CTCTTC(1/4)
KspI 330 CCGCGG CCGC^GG
MamI 2013 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1817 GGATCC [GA]^GATC[CT]
MseI 572 TTAA T^TAA
NaeI 102 GCCGGC GCC^GGC
NcoI 179 CCATGG C^CATGG
NdeI 65 CATATG CA^TATG
NgoMIV 102 GCCGGC G^CCGGC
PflMI 162 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 1839 TCATGA T^CATGA
SacII 330 CCGCGG CCGC^GG
SbfI 187 CCTGCAGG CCTGCA^GG
SmlI 1201 CTCAAG C^T[CT][GA]AG
Sse8387I 187 CCTGCAGG CCTGCA^GG
SspBI 323 TGTACA T^GTACA
StuI 1384 AGGCCT AGG^CCT
Tru9I 572 TTAA T^TAA
Tsp509I 1403 AATT ^AATT
Van91I 162 CCAGGTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1817 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 36 enzymes) #
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AccI 1363 GTCTAC GT^[AC][GT]AC
2388 GTCTAC
AcyI 292 GGCGCC G[GA]^CG[CT]C
1018 GGCGCC
AhdI 785 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1867 GACCTGGAGTC
AspEI 785 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1867 GACCTGGAGTC
AspI 948 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1357 GACTCAGTC
BalI 1447 TGGCCA TGG^CCA
2235 TGGCCA
BbeI 292 GGCGCC GGCGC^C
1018 GGCGCC
BbrPI 2028 CACGTG CAC^GTG
2128 CACGTG
BciVI 750 GTATCC GTATCC(6/5)
816 GTATCC
BfaI 611 CTAG C^TAG
1618 CTAG
BlpI 534 GCTGAGC GC^T[ACGT]AGC
1681 GCTGAGC
Bpu1102I 534 GCTGAGC GC^T[ACGT]AGC
1681 GCTGAGC
BsaHI 292 GGCGCC G[GA]^CG[CT]C
1018 GGCGCC
BsmAI 1355 GAGAC GTCTC(1/5)
1182 GTCTC
BsrFI 102 GCCGGC [GA]^CCGG[CT]
1427 ACCGGC
BstXI 2141 CCAGCGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2246 CCAGCTTCCTGG
CelII 534 GCTGAGC GC^T[ACGT]AGC
1681 GCTGAGC
Cfr10I 102 GCCGGC [GA]^CCGG[CT]
1427 ACCGGC
EaeI 1447 TGGCCA [CT]^GGCC[GA]
2235 TGGCCA
Eam1105I 785 GACAGGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1867 GACCTGGAGTC
Hin1I 292 GGCGCC G[GA]^CG[CT]C
1018 GGCGCC
KasI 292 GGCGCC G^GCGCC
1018 GGCGCC
MaeI 611 CTAG C^TAG
1618 CTAG
MboII 1343 TCTTC GAAGA(8/7)
1604 TCTTC
MluNI 1447 TGGCCA TGG^CCA
2235 TGGCCA
MscI 1447 TGGCCA TGG^CCA
2235 TGGCCA
NarI 292 GGCGCC GG^CGCC
1018 GGCGCC
PflFI 948 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1357 GACTCAGTC
PmaCI 2028 CACGTG CAC^GTG
2128 CACGTG
PmlI 2028 CACGTG CAC^GTG
2128 CACGTG
SfiI 379 GGCCCGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
658 GGCCCCTCTGGCC
SfoI 292 GGCGCC GGC^GCC
1018 GGCGCC
TaqI 1493 TCGA T^CGA
2021 TCGA
Tth111I 948 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1357 GACTCAGTC
TthHB8I 1493 TCGA T^CGA
2021 TCGA
XcmI 491 CCACAGCCACCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2591 CCACAGGCGTGATGG
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# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BanI BanII BbvI BcnI BglI
BmyI BpmI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TseI Tsp45I TspRI XmaCI
XmaI