Restriction map for
pFN21ASDA1886
(Linear) (Six-base) MAPSORT of: KIAA1886.seq Check: 2866 from: 1 to: 2760
KIAA1886.orf
With 182 enzymes: SgfI *
October 24, 2006 15:18 ..
AccI GT'mk_AC
Cuts at: 0 1364 2389 2760
Size: 1364 1025 371
Fragments arranged by size:
1364 1025 371
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 1555 1698 1813 2119 2363 2565 2760
Size: 305 1250 143 115 306 244 202 195
Fragments arranged by size:
1250 306 305 244 202 195 143 115
AfeI AGC'GCT
Cuts at: 0 2104 2760
Size: 2104 656
AflIII A'CryG_T
Cuts at: 0 1157 2760
Size: 1157 1603
AhdI GACnn_n'nnGTC
Cuts at: 0 790 1872 2760
Size: 790 1082 888
Fragments arranged by size:
1082 888 790
AleI CACnn'nnGTG
Cuts at: 0 130 2596 2760
Size: 130 2466 164
Fragments arranged by size:
2466 164 130
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 296 911 2760
Size: 296 615 1849
Fragments arranged by size:
1849 615 296
AlwNI CAG_nnn'CTG
Cuts at: 0 264 448 475 534 585 735 777 834
Size: 264 184 27 59 51 150 42 57
Cuts at: 834 957 2215 2760
Size: 123 1258 545
Fragments arranged by size:
1258 545 264 184 150 123 59 57
51 42 27
ApaI G_GGCC'C
Cuts at: 0 187 351 661 1748 2173 2487 2609 2704
Size: 187 164 310 1087 425 314 122 95
Cuts at: 2704 2760
Size: 56
Fragments arranged by size:
1087 425 314 310 187 164 122 95
56
ApaLI G'TGCA_C
Cuts at: 0 1621 2760
Size: 1621 1139
AvaI C'yCGr_G
Cuts at: 0 381 602 695 1086 1832 2325 2760
Size: 381 221 93 391 746 493 435
Fragments arranged by size:
746 493 435 391 381 221 93
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 733 766 2760
Size: 733 33 1994
Fragments arranged by size:
1994 733 33
BamHI G'GATC_C
Cuts at: 0 1817 2760
Size: 1817 943
BanI G'GyrC_C
Cuts at: 0 292 1018 1723 1735 2415 2760
Size: 292 726 705 12 680 345
Fragments arranged by size:
726 705 680 345 292 12
BanII G_rGCy'C
Cuts at: 0 187 351 661 1304 1748 2122 2173 2487
Size: 187 164 310 643 444 374 51 314
Cuts at: 2487 2609 2704 2760
Size: 122 95 56
Fragments arranged by size:
643 444 374 314 310 187 164 122
95 56 51
BbeI G_GCGC'C
Cuts at: 0 296 1022 2760
Size: 296 726 1738
Fragments arranged by size:
1738 726 296
BbsI GAAGACnn'nnnn_
Cuts at: 0 1335 2760
Size: 1335 1425
BciVI GTATCCnnnnn_n'
Cuts at: 0 761 827 2760
Size: 761 66 1933
Fragments arranged by size:
1933 761 66
BglI GCCn_nnn'nGGC
Cuts at: 0 335 386 502 665 1058 1553 2088 2632
Size: 335 51 116 163 393 495 535 544
Cuts at: 2632 2760
Size: 128
Fragments arranged by size:
544 535 495 393 335 163 128 116
51
BlpI GC'TnA_GC
Cuts at: 0 535 1682 2760
Size: 535 1147 1078
Fragments arranged by size:
1147 1078 535
Bme1580I G_kGCm'C
Cuts at: 0 187 351 661 923 1625 1748 1801 2051
Size: 187 164 310 262 702 123 53 250
Cuts at: 2051 2173 2487 2609 2704 2760
Size: 122 314 122 95 56
Fragments arranged by size:
702 314 310 262 250 187 164 123
122 122 95 56 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 861 945 1091 2760
Size: 861 84 146 1669
Fragments arranged by size:
1669 861 146 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 496 1318 1414 1891 1963 1972 2474 2642
Size: 496 822 96 477 72 9 502 168
Cuts at: 2642 2760
Size: 118
Fragments arranged by size:
822 502 496 477 168 118 96 72
9
Bpu10I CC'TnA_GC
Cuts at: 0 2556 2760
Size: 2556 204
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1186 2760
Size: 1186 1574
BsaAI yAC'GTr
Cuts at: 0 309 2030 2130 2760
Size: 309 1721 100 630
Fragments arranged by size:
1721 630 309 100
BsaBI GATnn'nnATC
Cuts at: 0 2017 2760
Size: 2017 743
BsaHI Gr'CG_yC
Cuts at: 0 293 1019 2760
Size: 293 726 1741
Fragments arranged by size:
1741 726 293
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1201 1231 2696 2726 2760
Size: 1201 30 1465 30 34
Fragments arranged by size:
1465 1201 34 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1648 2023 2092 2760
Size: 1648 375 69 668
Fragments arranged by size:
1648 668 375 69
BseYI C'CCAG_C
Cuts at: 0 770 856 877 1971 2140 2221 2296 2362
Size: 770 86 21 1094 169 81 75 66
Cuts at: 2362 2498 2523 2611 2760
Size: 136 25 88 149
Fragments arranged by size:
1094 770 169 149 136 88 86 81
75 66 25 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 331 1480 2052 2488 2760
Size: 331 1149 572 436 272
Fragments arranged by size:
1149 572 436 331 272
BsiHKAI G_wGCw'C
Cuts at: 0 1625 1688 2135 2760
Size: 1625 63 447 625
Fragments arranged by size:
1625 625 447 63
Bsp1286I G_dGCh'C
Cuts at: 0 187 351 661 923 1304 1625 1688 1748
Size: 187 164 310 262 381 321 63 60
Cuts at: 1748 1801 2051 2122 2135 2173 2487 2609 2704
Size: 53 250 71 13 38 314 122 95
Cuts at: 2704 2760
Size: 56
Fragments arranged by size:
381 321 314 310 262 250 187 164
122 95 71 63 60 56 53 38
13
BspHI T'CATG_A
Cuts at: 0 1839 2760
Size: 1839 921
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 33 1452 1889 2413 2663 2744 2760
Size: 33 1419 437 524 250 81 16
Fragments arranged by size:
1419 524 437 250 81 33 16
BsrBI CCG'CTC
Cuts at: 0 1485 1640 1899 2760
Size: 1485 155 259 861
Fragments arranged by size:
1485 861 259 155
BsrFI r'CCGG_y
Cuts at: 0 102 1427 2760
Size: 102 1325 1333
Fragments arranged by size:
1333 1325 102
BsrGI T'GTAC_A
Cuts at: 0 323 2760
Size: 323 2437
BssSI C'ACGA_G
Cuts at: 0 1420 2760
Size: 1420 1340
BstAPI GCAn_nnn'nTGC
Cuts at: 0 448 475 735 834 927 1620 2760
Size: 448 27 260 99 93 693 1140
Fragments arranged by size:
1140 693 448 260 99 93 27
BstEII G'GTnAC_C
Cuts at: 0 2425 2760
Size: 2425 335
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2148 2253 2760
Size: 2148 105 507
Fragments arranged by size:
2148 507 105
BstYI r'GATC_y
Cuts at: 0 1817 2760
Size: 1817 943
BtgI C'CryG_G
Cuts at: 0 179 330 500 974 2760
Size: 179 151 170 474 1786
Fragments arranged by size:
1786 474 179 170 151
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 565 1214 1298 2760
Size: 565 649 84 1462
Fragments arranged by size:
1462 649 565 84
BtsI GCAGTG_nn'
Cuts at: 0 473 775 1105 1686 2760
Size: 473 302 330 581 1074
Fragments arranged by size:
1074 581 473 330 302
DraIII CAC_nnn'GTG
Cuts at: 0 49 2760
Size: 49 2711
EaeI y'GGCC_r
Cuts at: 0 1447 2235 2760
Size: 1447 788 525
Fragments arranged by size:
1447 788 525
EarI CTCTTCn'nnn_
Cuts at: 0 1609 2760
Size: 1609 1151
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 231 1297 2760
Size: 231 1066 1463
Fragments arranged by size:
1463 1066 231
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 496 1318 1414 1555 1698 1813 1891
Size: 305 191 822 96 141 143 115 78
Cuts at: 1891 1963 1972 2119 2363 2474 2565 2642 2760
Size: 72 9 147 244 111 91 77 118
Fragments arranged by size:
822 305 244 191 147 143 141 118
115 111 96 91 78 77 72 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 937 1812 1832 2591 2760
Size: 937 875 20 759 169
Fragments arranged by size:
937 875 759 169 20
EcoO109I rG'GnC_Cy
Cuts at: 0 184 347 348 387 598 607 658 1653
Size: 184 163 1 39 211 9 51 995
Cuts at: 1653 1947 2170 2257 2483 2484 2494 2560 2605
Size: 294 223 87 226 1 10 66 45
Cuts at: 2605 2606 2634 2760
Size: 1 28 126
Fragments arranged by size:
995 294 226 223 211 184 163 126
87 66 51 45 39 28 10 9
1 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1203 1265 1728 2760
Size: 1203 62 463 1032
Fragments arranged by size:
1203 1032 463 62
HaeII r_GCGC'y
Cuts at: 0 95 296 1022 1671 2106 2180 2760
Size: 95 201 726 649 435 74 580
Fragments arranged by size:
726 649 580 435 201 95 74
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 776 808 1165 1197 1346 1378 1858 1890
Size: 776 32 357 32 149 32 480 32
Cuts at: 1890 2674 2706 2760
Size: 784 32 54
Fragments arranged by size:
784 776 480 357 149 54 32 32
32 32 32
KasI G'GCGC_C
Cuts at: 0 292 1018 2760
Size: 292 726 1742
Fragments arranged by size:
1742 726 292
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2498 2760
Size: 2498 262
MscI TGG'CCA
Cuts at: 0 1449 2237 2760
Size: 1449 788 523
Fragments arranged by size:
1449 788 523
MslI CAynn'nnrTG
Cuts at: 0 50 130 2596 2760
Size: 50 80 2466 164
Fragments arranged by size:
2466 164 80 50
MspA1I CmG'CkG
Cuts at: 0 332 445 454 622 1350 1721 2036 2502
Size: 332 113 9 168 728 371 315 466
Cuts at: 2502 2760
Size: 258
Fragments arranged by size:
728 466 371 332 315 258 168 113
9
NaeI GCC'GGC
Cuts at: 0 104 2760
Size: 104 2656
NarI GG'CG_CC
Cuts at: 0 293 1019 2760
Size: 293 726 1741
Fragments arranged by size:
1741 726 293
NcoI C'CATG_G
Cuts at: 0 179 2760
Size: 179 2581
NdeI CA'TA_TG
Cuts at: 0 66 2760
Size: 66 2694
NgoMIV G'CCGG_C
Cuts at: 0 102 2760
Size: 102 2658
NspI r_CATG'y
Cuts at: 0 409 1161 1543 1971 2067 2619 2760
Size: 409 752 382 428 96 552 141
Fragments arranged by size:
752 552 428 409 382 141 96
PasI CC'CwG_GG
Cuts at: 0 343 883 916 1741 2166 2760
Size: 343 540 33 825 425 594
Fragments arranged by size:
825 594 540 425 343 33
PciI A'CATG_T
Cuts at: 0 1157 2760
Size: 1157 1603
PflMI CCAn_nnn'nTGG
Cuts at: 0 168 2760
Size: 168 2592
PfoI T'CCnGG_A
Cuts at: 0 512 1295 2660 2760
Size: 512 783 1365 100
Fragments arranged by size:
1365 783 512 100
PmlI CAC'GTG
Cuts at: 0 2030 2130 2760
Size: 2030 100 630
Fragments arranged by size:
2030 630 100
PpuMI rG'GwC_Cy
Cuts at: 0 598 2494 2560 2760
Size: 598 1896 66 200
Fragments arranged by size:
1896 598 200 66
PsiI TTA'TAA
Cuts at: 0 989 2760
Size: 989 1771
PspOMI G'GGCC_C
Cuts at: 0 183 347 657 1744 2169 2483 2605 2700
Size: 183 164 310 1087 425 314 122 95
Cuts at: 2700 2760
Size: 60
Fragments arranged by size:
1087 425 314 310 183 164 122 95
60
PstI C_TGCA'G
Cuts at: 0 192 453 590 938 1316 1616 1652 1886
Size: 192 261 137 348 378 300 36 234
Cuts at: 1886 2201 2760
Size: 315 559
Fragments arranged by size:
559 378 348 315 300 261 234 192
137 36
PvuII CAG'CTG
Cuts at: 0 445 454 1350 2036 2502 2760
Size: 445 9 896 686 466 258
Fragments arranged by size:
896 686 466 445 258 9
SacII CC_GC'GG
Cuts at: 0 333 2760
Size: 333 2427
SbfI CC_TGCA'GG
Cuts at: 0 192 2760
Size: 192 2568
SfcI C'TryA_G
Cuts at: 0 188 449 586 934 1047 1312 1612 1648
Size: 188 261 137 348 113 265 300 36
Cuts at: 1648 1882 2197 2760
Size: 234 315 563
Fragments arranged by size:
563 348 315 300 265 261 234 188
137 113 36
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 386 665 2760
Size: 386 279 2095
Fragments arranged by size:
2095 386 279
SfoI GGC'GCC
Cuts at: 0 294 1020 2760
Size: 294 726 1740
Fragments arranged by size:
1740 726 294
SmaI CCC'GGG
Cuts at: 0 383 604 697 1088 2327 2760
Size: 383 221 93 391 1239 433
Fragments arranged by size:
1239 433 391 383 221 93
SmlI C'TyrA_G
Cuts at: 0 1201 2760
Size: 1201 1559
StuI AGG'CCT
Cuts at: 0 1386 2760
Size: 1386 1374
StyI C'CwwG_G
Cuts at: 0 79 179 677 2478 2540 2760
Size: 79 100 498 1801 62 220
Fragments arranged by size:
1801 498 220 100 79 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 870 1143 2301 2760
Size: 870 273 1158 459
Fragments arranged by size:
1158 870 459 273
TatI w'GTAC_w
Cuts at: 0 323 1964 2760
Size: 323 1641 796
Fragments arranged by size:
1641 796 323
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1615 1647 2430 2462 2696 2728 2760
Size: 1615 32 783 32 234 32 32
Fragments arranged by size:
1615 783 234 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 951 1360 2760
Size: 951 409 1400
Fragments arranged by size:
1400 951 409
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 498 2598 2760
Size: 498 2100 162
Fragments arranged by size:
2100 498 162
XmaI C'CCGG_G
Cuts at: 0 381 602 695 1086 2325 2760
Size: 381 221 93 391 1239 435
Fragments arranged by size:
1239 435 391 381 221 93
Enzymes that do cut:
AccI AcuI AfeI AflIII AhdI AleI AlfI AlwNI
ApaI ApaLI AvaI BaeI BamHI BanI BanII BbeI
BbsI BciVI BglI BlpI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BstEII BstXI BstYI BtgI BtgZI BtsI DraIII
EaeI EarI EciI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I KasI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI
PmlI PpuMI PsiI PspOMI PstI PvuII SacII SbfI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AflII AgeI AloI ApoI
AscI AseI AsiSI AvrII BbvCI BcgI BclI BfrBI
BglII BmgBI BmtI BplI BsaI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BsrDI BssHII BstBI BstZ17I Bsu36I
ClaI CspCI DraI DrdI EagI EcoICRI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI NheI NotI NruI NsiI PacI PmeI PpiI
PshAI PspXI PsrI PvuI RsrII SacI SalI SanDI
SapI ScaI SexAI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI XbaI XhoI XmnI ZraI