(Circular) (Six-base) MAPSORT of: pFN21ASDA1874.seq Check: 8333 from: 1 to: 6292 pFN21ASDA1874 6292 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4102 278 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2601 3635 1937 Size: 664 1034 4594 Fragments arranged by size: 4594 1034 664 Acc65I G'GTAC_C Cuts at: 3613 3613 Size: 6292 AclI AA'CG_TT Cuts at: 1563 4419 4792 1563 Size: 2856 373 3063 Fragments arranged by size: 3063 2856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2376 2661 3346 3594 4355 5739 6007 1632 Size: 744 285 685 248 761 1384 268 1917 Fragments arranged by size: 1917 1384 761 744 685 285 268 248 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 5191 6168 1203 Size: 3988 977 1327 Fragments arranged by size: 3988 1327 977 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 5021 5021 Size: 6292 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2437 1184 Size: 1253 5039 AlwNI CAG_nnn'CTG Cuts at: 4035 5607 4035 Size: 1572 4720 ApaLI G'TGCA_C Cuts at: 4349 5505 4349 Size: 1156 5136 ApoI r'AATT_y Cuts at: 1493 2563 2907 2976 3312 3396 3601 3827 3979 Size: 1070 344 69 336 84 205 226 152 Cuts at: 3979 6088 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 1070 344 336 226 205 152 84 69 AseI AT'TA_AT Cuts at: 160 1032 4846 160 Size: 872 3814 1606 Fragments arranged by size: 3814 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 3617 1870 Size: 73 1674 4545 Fragments arranged by size: 4545 1674 73 BamHI G'GATC_C Cuts at: 2209 3622 3989 2209 Size: 1413 367 4512 Fragments arranged by size: 4512 1413 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3613 5068 618 Size: 325 217 627 1826 1455 1842 Fragments arranged by size: 1842 1826 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2062 3374 3611 1391 Size: 671 1312 237 4072 Fragments arranged by size: 4072 1312 671 237 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2058 2649 928 Size: 971 159 591 4571 Fragments arranged by size: 4571 971 591 159 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4483 4517 1903 Size: 34 2546 34 3678 Fragments arranged by size: 3678 2546 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4184 5394 4184 Size: 1210 5082 BclI T'GATC_A Cuts at: 1548 1548 Size: 6292 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4903 136 Size: 107 122 71 631 605 3231 1525 Fragments arranged by size: 3231 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6287 6287 Size: 6292 BlpI GC'TnA_GC Cuts at: 3709 3709 Size: 6292 Bme1580I G_kGCm'C Cuts at: 1790 4353 5509 1790 Size: 2563 1156 2573 Fragments arranged by size: 2573 2563 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6292 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4023 4981 442 Size: 416 53 83 399 24 2606 958 1753 Fragments arranged by size: 2606 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2255 2841 3090 3174 3258 3342 4952 1239 Size: 1016 586 249 84 84 84 1610 2579 Fragments arranged by size: 2579 1610 1016 586 249 84 84 84 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2345 4417 5282 5580 2345 Size: 2072 865 298 3057 Fragments arranged by size: 3057 2072 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2986 4955 882 Size: 617 1487 1969 2219 Fragments arranged by size: 2219 1969 1487 617 BsaAI yAC'GTr Cuts at: 493 1204 3589 4083 493 Size: 711 2385 494 2702 Fragments arranged by size: 2702 2385 711 494 BsaBI GATnn'nnATC Cuts at: 6286 6286 Size: 6292 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4099 4481 5116 275 Size: 53 83 186 936 2566 382 635 1451 Fragments arranged by size: 2566 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3488 4725 5123 5397 5544 5955 3488 Size: 1237 398 274 147 411 3825 Fragments arranged by size: 3825 1237 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6067 Fragments arranged by size: 6067 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2207 1186 Size: 1021 5271 BseYI C'CCAG_C Cuts at: 5495 5991 5495 Size: 496 5796 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2199 3068 3556 1163 Size: 573 463 869 488 3899 Fragments arranged by size: 3899 869 573 488 463 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3742 4503 5531 1540 Size: 275 186 1741 761 1028 2301 Fragments arranged by size: 2301 1741 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2062 2102 3290 3374 4353 4438 5509 2062 Size: 40 1188 84 979 85 1071 2845 Fragments arranged by size: 2845 1188 1071 979 85 84 40 BsmI GAATG_Cn' Cuts at: 2924 2933 3008 3260 3803 3896 2924 Size: 9 75 252 543 93 5320 Fragments arranged by size: 5320 543 252 93 75 9 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2062 2102 3290 3374 3611 4353 4438 Size: 399 272 40 1188 84 237 742 85 Cuts at: 4438 5509 1391 Size: 1071 2174 Fragments arranged by size: 2174 1188 1071 742 399 272 237 85 84 40 BspEI T'CCGG_A Cuts at: 5955 5955 Size: 6292 BspHI T'CATG_A Cuts at: 4181 5911 4181 Size: 1730 4562 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2846 3647 844 Size: 2002 801 3489 Fragments arranged by size: 3489 2002 801 BsrBI CCG'CTC Cuts at: 1394 1823 4179 1394 Size: 429 2356 3507 Fragments arranged by size: 3507 2356 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2848 3013 3199 4787 4961 65 Size: 1183 1600 165 186 1588 174 1396 Fragments arranged by size: 1600 1588 1396 1183 186 174 165 BsrFI r'CCGG_y Cuts at: 1700 1810 4936 5123 1700 Size: 110 3126 187 2869 Fragments arranged by size: 3126 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6292 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 4352 5364 4352 Size: 1012 5280 BstAPI GCAn_nnn'nTGC Cuts at: 794 2928 794 Size: 2134 4158 BstBI TT'CG_AA Cuts at: 2007 5096 2007 Size: 3089 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6292 BstXI CCAn_nnnn'nTGG Cuts at: 2196 2196 Size: 6292 BstYI r'GATC_y Cuts at: 1972 2141 2209 2660 3622 3989 4374 4391 5843 Size: 169 68 451 962 367 385 17 1452 Cuts at: 5843 5929 5941 6287 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 962 451 385 367 346 169 86 68 17 12 BstZ17I GTA'TAC Cuts at: 2602 2602 Size: 6292 BtgI C'CryG_G Cuts at: 513 1065 2022 2175 513 Size: 552 957 153 4630 Fragments arranged by size: 4630 957 552 153 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5553 Fragments arranged by size: 5553 683 56 BtsI GCAGTG_nn' Cuts at: 2929 3265 3808 4602 4622 2929 Size: 336 543 794 20 4599 Fragments arranged by size: 4599 794 543 336 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2830 2865 2995 3030 529 Size: 35 2266 35 130 35 3791 Fragments arranged by size: 3791 2266 130 35 35 35 DraI TTT'AAA Cuts at: 3596 3596 Size: 6292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5299 809 Size: 507 3983 1802 Fragments arranged by size: 3983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2136 2172 3739 Size: 54 1425 50 275 18 306 36 1567 Cuts at: 3739 4628 6257 8 Size: 889 1629 43 Fragments arranged by size: 1629 1567 1425 889 306 275 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 3739 1537 Size: 275 1927 4090 Fragments arranged by size: 4090 1927 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2090 2100 2780 3570 4222 1353 Size: 737 10 680 790 652 3423 Fragments arranged by size: 3423 790 737 680 652 10 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4879 5253 5399 387 Size: 1064 138 3290 374 146 1280 Fragments arranged by size: 3290 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2060 3372 2060 Size: 1312 4980 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 2376 2661 2841 3090 3174 3258 Size: 393 623 121 285 180 249 84 84 Cuts at: 3258 3342 3346 3594 4355 4952 5739 6007 1239 Size: 84 4 248 761 597 787 268 1524 Fragments arranged by size: 1524 787 761 623 597 393 285 268 249 248 180 121 84 84 84 4 EcoNI CCTnn'n_nnAGG Cuts at: 2052 2052 Size: 6292 EcoRI G'AATT_C Cuts at: 2563 2907 2976 3312 3396 3601 3979 2563 Size: 344 69 336 84 205 378 4876 Fragments arranged by size: 4876 378 344 336 205 84 69 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2504 2956 2504 Size: 452 5840 FspI TGC'GCA Cuts at: 4798 4798 Size: 6292 HaeII r_GCGC'y Cuts at: 1678 5439 1678 Size: 3761 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3277 3309 4933 4965 5007 Size: 32 135 32 1705 32 1624 32 42 Cuts at: 5007 5039 1373 Size: 32 2626 Fragments arranged by size: 2626 1705 1624 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3636 3882 669 Size: 988 281 1698 246 3079 Fragments arranged by size: 3079 1698 988 281 246 HindIII A'AGCT_T Cuts at: 748 2321 2611 2689 3105 3526 748 Size: 1573 290 78 416 421 3514 Fragments arranged by size: 3514 1573 421 416 290 78 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KpnI G_GTAC'C Cuts at: 3617 3617 Size: 6292 MfeI C'AATT_G Cuts at: 3891 3891 Size: 6292 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2965 5406 5590 1305 Size: 1660 2441 184 2007 Fragments arranged by size: 2441 2007 1660 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2174 10 Size: 54 1425 343 342 4128 Fragments arranged by size: 4128 1425 343 342 54 MslI CAynn'nnrTG Cuts at: 518 1166 2558 2592 3253 4250 4609 4768 518 Size: 648 1392 34 661 997 359 159 2042 Fragments arranged by size: 2042 1392 997 661 648 359 159 34 MspA1I CmG'CkG Cuts at: 1606 3708 4385 5533 5778 1606 Size: 2102 677 1148 245 2120 Fragments arranged by size: 2120 2102 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2175 513 Size: 552 957 153 4630 Fragments arranged by size: 4630 957 552 153 NdeI CA'TA_TG Cuts at: 387 3250 387 Size: 2863 3429 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 3650 5195 1132 Size: 97 2421 1545 2229 Fragments arranged by size: 2421 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6292 PciI A'CATG_T Cuts at: 5191 5191 Size: 6292 PflMI CCAn_nnn'nTGG Cuts at: 1108 2296 1108 Size: 1188 5104 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2240 2272 4361 4393 5874 5906 2240 Size: 32 2089 32 1481 32 2626 Fragments arranged by size: 2626 2089 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 2733 3168 3862 2733 Size: 435 694 5163 Fragments arranged by size: 5163 694 435 PstI C_TGCA'G Cuts at: 830 3644 830 Size: 2814 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 SacI G_AGCT'C Cuts at: 2062 3374 2062 Size: 1312 4980 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SapI GCTCTTCn'nnn_ Cuts at: 2090 3570 2090 Size: 1480 4812 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 4540 6252 4540 Size: 1712 4580 SfcI C'TryA_G Cuts at: 826 1045 2242 3640 4775 5456 5647 826 Size: 219 1197 1398 1135 681 191 1471 Fragments arranged by size: 1471 1398 1197 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 3619 1872 Size: 1747 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2324 4396 5297 5559 820 Size: 197 926 381 2072 901 262 1553 Fragments arranged by size: 2072 1553 926 901 381 262 197 SnaBI TAC'GTA Cuts at: 493 3589 4083 493 Size: 3096 494 2702 Fragments arranged by size: 3096 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 3650 1132 Size: 2518 3774 SspI AAT'ATT Cuts at: 5 52 4216 5 Size: 47 4164 2081 Fragments arranged by size: 4164 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6292 StyI C'CwwG_G Cuts at: 513 1065 2022 2175 3731 513 Size: 552 957 153 1556 3074 Fragments arranged by size: 3074 1556 957 552 153 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4319 4336 4489 4674 5119 325 Size: 1255 2739 17 153 185 445 1498 Fragments arranged by size: 2739 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3035 4538 6250 Size: 274 80 33 51 1443 1058 1503 1712 Cuts at: 6250 96 Size: 138 Fragments arranged by size: 1712 1503 1443 1058 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6252 6284 6252 Size: 32 6260 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6292 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6292 XmaI C'CCGG_G Cuts at: 1870 3617 1870 Size: 1747 4545 XmnI GAAnn'nnTTC Cuts at: 2612 2939 3359 4421 2612 Size: 327 420 1062 4483 Fragments arranged by size: 4483 1062 420 327 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4100 276 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI Bsu36I DraIII EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI