(Circular) (Six-base) MAPSORT of: pFN21ASDA1862.seq Check: 1002 from: 1 to: 7819 pFN21ASDA1862 7819 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5629 278 Size: 53 83 186 936 4093 2468 Fragments arranged by size: 4093 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 5162 1937 Size: 3225 4594 Acc65I G'GTAC_C Cuts at: 5140 5140 Size: 7819 AclI AA'CG_TT Cuts at: 1563 5946 6319 1563 Size: 4383 373 3063 Fragments arranged by size: 4383 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2687 2870 3215 3949 3996 5882 7266 7534 Size: 1055 183 345 734 47 1886 1384 268 Cuts at: 7534 1632 Size: 1917 Fragments arranged by size: 1917 1886 1384 1055 734 345 268 183 47 AfeI AGC'GCT Cuts at: 4482 4482 Size: 7819 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7622 AflIII A'CryG_T Cuts at: 1203 6718 7695 1203 Size: 5515 977 1327 Fragments arranged by size: 5515 1327 977 AgeI A'CCGG_T Cuts at: 6650 6650 Size: 7819 AhdI GACnn_n'nnGTC Cuts at: 3277 6548 3277 Size: 3271 4548 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7819 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2169 1184 Size: 985 6834 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2926 2958 2926 Size: 32 7787 AlwNI CAG_nnn'CTG Cuts at: 2105 2836 3053 3098 3529 3630 4001 4149 4429 Size: 731 217 45 431 101 371 148 280 Cuts at: 4429 4822 4825 5562 7134 2105 Size: 393 3 737 1572 2790 Fragments arranged by size: 2790 1572 737 731 431 393 371 280 217 148 101 45 3 ApaI G_GGCC'C Cuts at: 2691 3137 4307 5059 2691 Size: 446 1170 752 5451 Fragments arranged by size: 5451 1170 752 446 ApaLI G'TGCA_C Cuts at: 5876 7032 5876 Size: 1156 6663 ApoI r'AATT_y Cuts at: 1493 2622 5128 5354 5506 7615 1493 Size: 1129 2506 226 152 2109 1697 Fragments arranged by size: 2506 2109 1697 1129 226 152 AseI AT'TA_AT Cuts at: 160 1032 6373 160 Size: 872 5341 1606 Fragments arranged by size: 5341 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7819 AvaI C'yCGr_G Cuts at: 1870 1943 2279 2327 2699 2861 3399 3683 3958 Size: 73 336 48 372 162 538 284 275 Cuts at: 3958 3988 5095 5144 1870 Size: 30 1107 49 4545 Fragments arranged by size: 4545 1107 538 372 336 284 275 162 73 49 48 30 BamHI G'GATC_C Cuts at: 4180 5004 5149 5516 4180 Size: 824 145 367 6483 Fragments arranged by size: 6483 824 367 145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2694 4542 4993 5140 6595 Size: 325 217 627 907 1848 451 147 1455 Cuts at: 6595 618 Size: 1842 Fragments arranged by size: 1848 1842 1455 907 627 451 325 217 147 BanII G_rGCy'C Cuts at: 1391 2327 2352 2536 2550 2691 2783 2859 3044 Size: 936 25 184 14 141 92 76 185 Cuts at: 3044 3137 3624 4008 4143 4307 4615 4800 4901 Size: 93 487 384 135 164 308 185 101 Cuts at: 4901 5059 5138 1391 Size: 158 79 4072 Fragments arranged by size: 4072 936 487 384 308 185 185 184 164 158 141 135 101 93 92 79 76 25 14 BbeI G_GCGC'C Cuts at: 4546 4546 Size: 7819 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2804 3990 4128 4264 928 Size: 971 905 1186 138 136 4483 Fragments arranged by size: 4483 1186 971 905 138 136 BbvCI CC'TCA_GC Cuts at: 3091 3589 3660 3091 Size: 498 71 7250 Fragments arranged by size: 7250 498 71 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2318 2352 4141 4175 5017 5051 6010 Size: 34 381 34 1789 34 842 34 959 Cuts at: 6010 6044 1903 Size: 34 3678 Fragments arranged by size: 3678 1789 959 842 381 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2951 5711 6921 2951 Size: 2760 1210 3849 Fragments arranged by size: 3849 2760 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7819 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3073 3577 3898 Size: 107 122 71 631 605 1401 504 321 Cuts at: 3898 4551 6430 136 Size: 653 1879 1525 Fragments arranged by size: 1879 1525 1401 653 631 605 504 321 122 107 71 BglII A'GATC_T Cuts at: 7814 7814 Size: 7819 BlpI GC'TnA_GC Cuts at: 2064 2372 5236 2064 Size: 308 2864 4647 Fragments arranged by size: 4647 2864 308 Bme1580I G_kGCm'C Cuts at: 1790 2691 2744 3137 4307 4756 5059 5880 7036 Size: 901 53 393 1170 449 303 821 1156 Cuts at: 7036 1790 Size: 2573 Fragments arranged by size: 2573 1170 1156 901 821 449 393 303 53 BmgBI CAC'GTC Cuts at: 1318 2745 4757 4975 1318 Size: 1427 2012 218 4162 Fragments arranged by size: 4162 2012 1427 218 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2877 3451 4314 Size: 416 53 83 399 24 1460 574 863 Cuts at: 4314 5550 6508 442 Size: 1236 958 1753 Fragments arranged by size: 1753 1460 1236 958 863 574 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7819 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2387 2387 Size: 7819 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2813 2967 3120 3551 3733 3887 3937 4223 Size: 1574 154 153 431 182 154 50 286 Cuts at: 4223 4262 4666 4717 4752 5045 6479 1239 Size: 39 404 51 35 293 1434 2579 Fragments arranged by size: 2579 1574 1434 431 404 293 286 182 154 154 153 51 50 39 35 Bpu10I CC'TnA_GC Cuts at: 3091 3589 3660 4002 4176 4311 4497 3091 Size: 498 71 342 174 135 186 6413 Fragments arranged by size: 6413 498 342 186 174 135 71 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3129 3145 3527 5944 6809 7107 3129 Size: 16 382 2417 865 298 3841 Fragments arranged by size: 3841 2417 865 382 298 16 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2053 3285 6482 882 Size: 617 554 1232 3197 2219 Fragments arranged by size: 3197 2219 1232 617 554 BsaAI yAC'GTr Cuts at: 493 1204 5116 5610 493 Size: 711 3912 494 2702 Fragments arranged by size: 3912 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7813 7813 Size: 7819 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4543 5626 6008 6643 Size: 53 83 186 936 3010 1083 382 635 Cuts at: 6643 275 Size: 1451 Fragments arranged by size: 3010 1451 1083 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4336 4351 6252 6650 6924 7071 7482 4336 Size: 15 1901 398 274 147 411 4673 Fragments arranged by size: 4673 1901 411 398 274 147 15 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2959 2989 4854 4884 1178 Size: 30 165 30 1556 30 1865 30 4113 Fragments arranged by size: 4113 1865 1556 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2964 3412 3577 3625 4372 4862 4938 5058 Size: 1778 448 165 48 747 490 76 120 Cuts at: 5058 1186 Size: 3947 Fragments arranged by size: 3947 1778 747 490 448 165 120 76 48 BseYI C'CCAG_C Cuts at: 2549 2570 3030 3125 3129 3228 3261 3380 3929 Size: 21 460 95 4 99 33 119 549 Cuts at: 3929 3935 3941 4043 4534 4608 4815 4838 5038 Size: 6 6 102 491 74 207 23 200 Cuts at: 5038 7022 7518 2549 Size: 1984 496 2850 Fragments arranged by size: 2850 1984 549 496 491 460 207 200 119 102 99 95 74 33 23 21 6 6 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2183 2237 3842 4185 4415 4845 4983 Size: 573 447 54 1605 343 230 430 138 Cuts at: 4983 5069 1163 Size: 86 3913 Fragments arranged by size: 3913 1605 573 447 430 343 230 138 86 54 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5269 6030 7058 1540 Size: 275 186 3268 761 1028 2301 Fragments arranged by size: 3268 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2859 4008 5088 5880 5965 7036 2859 Size: 1149 1080 792 85 1071 3642 Fragments arranged by size: 3642 1149 1080 1071 792 85 BsmI GAATG_Cn' Cuts at: 3201 5330 5423 3201 Size: 2129 93 5597 Fragments arranged by size: 5597 2129 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2033 3686 2033 Size: 1653 6166 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2327 2352 2536 2550 2691 2744 2783 Size: 399 537 25 184 14 141 53 39 Cuts at: 2783 2859 3044 3137 3624 4008 4143 4307 4615 Size: 76 185 93 487 384 135 164 308 Cuts at: 4615 4756 4800 4901 5059 5088 5138 5880 5965 Size: 141 44 101 158 29 50 742 85 Cuts at: 5965 7036 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 537 487 399 384 308 185 184 164 158 141 141 135 101 93 85 76 53 50 44 39 29 25 14 BspEI T'CCGG_A Cuts at: 4336 4351 7482 4336 Size: 15 3131 4673 Fragments arranged by size: 4673 3131 15 BspHI T'CATG_A Cuts at: 5708 7438 5708 Size: 1730 6089 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3061 4157 4728 5174 844 Size: 2217 1096 571 446 3489 Fragments arranged by size: 3489 2217 1096 571 446 BsrBI CCG'CTC Cuts at: 1394 1823 4018 4745 5014 5706 1394 Size: 429 2195 727 269 692 3507 Fragments arranged by size: 3507 2195 727 692 429 269 BsrDI GCAATG_nn' Cuts at: 65 1248 6314 6488 65 Size: 1183 5066 174 1396 Fragments arranged by size: 5066 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6463 6650 1700 Size: 110 4653 187 2869 Fragments arranged by size: 4653 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7819 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7819 BssSI C'ACGA_G Cuts at: 2434 4663 5879 6891 2434 Size: 2229 1216 1012 3362 Fragments arranged by size: 3362 2229 1216 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2197 4429 4961 794 Size: 1403 2232 532 3652 Fragments arranged by size: 3652 2232 1403 532 BstBI TT'CG_AA Cuts at: 2007 6623 2007 Size: 4616 3203 BstEII G'GTnAC_C Cuts at: 1184 4715 1184 Size: 3531 4288 BstXI CCAn_nnnn'nTGG Cuts at: 2931 3813 4240 4483 4556 2931 Size: 882 427 243 73 6194 Fragments arranged by size: 6194 882 427 243 73 BstYI r'GATC_y Cuts at: 1972 2167 2232 3885 4180 4680 5004 5149 5516 Size: 195 65 1653 295 500 324 145 367 Cuts at: 5516 5901 5918 7370 7456 7468 7814 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1653 1452 500 385 367 346 324 295 195 145 86 65 17 12 Bsu36I CC'TnA_GG Cuts at: 2890 3138 3878 4984 2890 Size: 248 740 1106 5725 Fragments arranged by size: 5725 1106 740 248 BtgI C'CryG_G Cuts at: 513 1065 2504 4595 513 Size: 552 1439 2091 3737 Fragments arranged by size: 3737 2091 1439 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7080 Fragments arranged by size: 7080 683 56 BtsI GCAGTG_nn' Cuts at: 2799 3518 3859 4108 4801 5335 6129 6149 2799 Size: 719 341 249 693 534 794 20 4469 Fragments arranged by size: 4469 794 719 693 534 341 249 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7819 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7784 DraI TTT'AAA Cuts at: 5123 5123 Size: 7819 DraIII CAC_nnn'GTG Cuts at: 3317 3317 Size: 7819 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6826 809 Size: 507 5510 1802 Fragments arranged by size: 5510 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2412 4506 4690 Size: 54 1425 50 275 18 582 2094 184 Cuts at: 4690 5266 6155 7784 8 Size: 576 889 1629 43 Fragments arranged by size: 2094 1629 1425 889 582 576 275 184 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5266 1537 Size: 275 3454 4090 Fragments arranged by size: 4090 3454 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2104 2576 4816 5749 1353 Size: 751 472 2240 933 3423 Fragments arranged by size: 3423 2240 933 751 472 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6406 6780 6926 387 Size: 1064 138 4817 374 146 1280 Fragments arranged by size: 4817 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2857 4006 2857 Size: 1149 6670 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2687 2813 2870 2967 3120 3215 3551 Size: 393 1055 126 57 97 153 95 336 Cuts at: 3551 3733 3887 3937 3949 3996 4223 4262 4666 Size: 182 154 50 12 47 227 39 404 Cuts at: 4666 4717 4752 5045 5882 6479 7266 7534 1239 Size: 51 35 293 837 597 787 268 1524 Fragments arranged by size: 1524 1055 837 787 597 404 393 336 293 268 227 182 154 153 126 97 95 57 51 50 47 39 35 12 EcoNI CCTnn'n_nnAGG Cuts at: 2361 2935 3492 4421 4496 4645 2361 Size: 574 557 929 75 149 5535 Fragments arranged by size: 5535 929 574 557 149 75 EcoO109I rG'GnC_Cy Cuts at: 2607 2688 2771 2951 3050 3115 3133 3134 3387 Size: 81 83 180 99 65 18 1 253 Cuts at: 3387 3445 3900 3926 4086 4303 4518 4553 4601 Size: 58 455 26 160 217 215 35 48 Cuts at: 4601 4658 5055 5056 5092 2607 Size: 57 397 1 36 5334 Fragments arranged by size: 5334 455 397 253 217 215 180 160 99 83 81 65 58 57 48 36 35 26 18 1 1 EcoRI G'AATT_C Cuts at: 5128 5506 5128 Size: 378 7441 FspI TGC'GCA Cuts at: 6325 6325 Size: 7819 HaeII r_GCGC'y Cuts at: 1678 2268 3707 3876 4484 4546 6966 1678 Size: 590 1439 169 608 62 2420 2531 Fragments arranged by size: 2531 2420 1439 608 590 169 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2959 2991 3263 3295 3706 Size: 32 135 32 1387 32 272 32 411 Cuts at: 3706 3738 6460 6492 6534 6566 1373 Size: 32 2722 32 42 32 2626 Fragments arranged by size: 2722 2626 1387 411 272 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 5163 5409 669 Size: 988 281 3225 246 3079 Fragments arranged by size: 3225 3079 988 281 246 HindIII A'AGCT_T Cuts at: 748 2713 4572 748 Size: 1965 1859 3995 Fragments arranged by size: 3995 1965 1859 HpaI GTT'AAC Cuts at: 5409 5409 Size: 7819 KasI G'GCGC_C Cuts at: 4542 4542 Size: 7819 KpnI G_GTAC'C Cuts at: 5144 5144 Size: 7819 MfeI C'AATT_G Cuts at: 5418 5418 Size: 7819 MluI A'CGCG_T Cuts at: 7695 7695 Size: 7819 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6933 7117 1305 Size: 5628 184 2007 Fragments arranged by size: 5628 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2414 4508 4692 10 Size: 54 1425 343 582 2094 184 3137 Fragments arranged by size: 3137 2094 1425 582 343 184 54 MslI CAynn'nnrTG Cuts at: 518 1166 2136 5777 6136 6295 518 Size: 648 970 3641 359 159 2042 Fragments arranged by size: 3641 2042 970 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2063 2221 2336 2376 3030 3129 3172 3232 Size: 457 158 115 40 654 99 43 60 Cuts at: 3232 3753 3775 3856 3969 4047 4155 4201 4762 Size: 521 22 81 113 78 108 46 561 Cuts at: 4762 4822 4842 5235 5912 7060 7305 1606 Size: 60 20 393 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 654 561 521 457 393 245 158 115 113 108 99 81 78 60 60 46 43 40 22 20 NarI GG'CG_CC Cuts at: 4543 4543 Size: 7819 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7267 NdeI CA'TA_TG Cuts at: 387 387 Size: 7819 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7819 NotI GC'GGCC_GC Cuts at: 5266 5266 Size: 7819 NspI r_CATG'y Cuts at: 1132 1229 5177 6722 1132 Size: 97 3948 1545 2229 Fragments arranged by size: 3948 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2173 2536 2787 3791 4215 4300 4556 1348 Size: 825 363 251 1004 424 85 256 4611 Fragments arranged by size: 4611 1004 825 424 363 256 251 85 PciI A'CATG_T Cuts at: 6718 6718 Size: 7819 PflMI CCAn_nnn'nTGG Cuts at: 1108 3630 4079 1108 Size: 2522 449 4848 Fragments arranged by size: 4848 2522 449 PfoI T'CCnGG_A Cuts at: 2923 3487 4577 5061 2923 Size: 564 1090 484 5681 Fragments arranged by size: 5681 1090 564 484 PmeI GTTT'AAAC Cuts at: 5123 5123 Size: 7819 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3324 3356 5888 5920 7401 7433 3324 Size: 32 2532 32 1481 32 3710 Fragments arranged by size: 3710 2532 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2607 3050 3115 3445 4086 4518 4601 4658 5092 Size: 443 65 330 641 432 83 57 434 Cuts at: 5092 2607 Size: 5334 Fragments arranged by size: 5334 641 443 434 432 330 83 65 57 PshAI GACnn'nnGTC Cuts at: 1613 2045 1613 Size: 432 7387 PsiI TTA'TAA Cuts at: 5389 5389 Size: 7819 PspOMI G'GGCC_C Cuts at: 2687 3133 4303 5055 2687 Size: 446 1170 752 5451 Fragments arranged by size: 5451 1170 752 446 PspXI vC'TCGA_Gb Cuts at: 3958 3958 Size: 7819 PstI C_TGCA'G Cuts at: 830 3360 3525 4154 4392 4422 4709 4808 4938 Size: 2530 165 629 238 30 287 99 130 Cuts at: 4938 4957 5171 830 Size: 19 214 3478 Fragments arranged by size: 3478 2530 629 287 238 214 165 130 99 30 19 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7819 PvuII CAG'CTG Cuts at: 2063 2221 2336 2376 3030 3129 3232 3753 4047 Size: 158 115 40 654 99 103 521 294 Cuts at: 4047 4201 4822 4842 2063 Size: 154 621 20 5040 Fragments arranged by size: 5040 654 621 521 294 158 154 115 103 99 40 20 SacI G_AGCT'C Cuts at: 2859 4008 2859 Size: 1149 6670 SalI G'TCGA_C Cuts at: 1936 5161 1936 Size: 3225 4594 SanDI GG'GwC_CC Cuts at: 3115 4086 4658 5092 3115 Size: 971 572 434 5842 Fragments arranged by size: 5842 971 572 434 SapI GCTCTTCn'nnn_ Cuts at: 2104 2576 4816 2104 Size: 472 2240 5107 Fragments arranged by size: 5107 2240 472 SbfI CC_TGCA'GG Cuts at: 4938 5171 4938 Size: 233 7586 ScaI AGT'ACT Cuts at: 6067 7779 6067 Size: 1712 6107 SfcI C'TryA_G Cuts at: 826 1045 2912 3356 3521 4150 4388 4418 4705 Size: 219 1867 444 165 629 238 30 287 Cuts at: 4705 4804 4934 4953 5167 6302 6983 7174 826 Size: 99 130 19 214 1135 681 191 1471 Fragments arranged by size: 1867 1471 1135 681 629 444 287 238 219 214 191 165 130 99 30 19 SfiI GGCCn_nnn'nGGCC Cuts at: 3577 3577 Size: 7819 SfoI GGC'GCC Cuts at: 4544 4544 Size: 7819 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7819 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7819 SmaI CCC'GGG Cuts at: 1872 5146 1872 Size: 3274 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3144 3160 3542 3958 5923 6824 Size: 197 926 1201 16 382 416 1965 901 Cuts at: 6824 7086 820 Size: 262 1553 Fragments arranged by size: 1965 1553 1201 926 901 416 382 262 197 16 SnaBI TAC'GTA Cuts at: 493 5116 5610 493 Size: 4623 494 2702 Fragments arranged by size: 4623 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7819 SphI G_CATG'C Cuts at: 1132 5177 1132 Size: 4045 3774 SspI AAT'ATT Cuts at: 5 52 5743 5 Size: 47 5691 2081 Fragments arranged by size: 5691 2081 47 StuI AGG'CCT Cuts at: 1517 2367 2652 3665 4239 4491 4669 1517 Size: 850 285 1013 574 252 178 4667 Fragments arranged by size: 4667 1013 850 574 285 252 178 StyI C'CwwG_G Cuts at: 513 1065 4549 5019 5258 513 Size: 552 3484 470 239 3074 Fragments arranged by size: 3484 3074 552 470 239 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2576 2816 3253 3622 4852 5846 5863 Size: 1255 996 240 437 369 1230 994 17 Cuts at: 5863 6016 6201 6646 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 1230 996 994 445 437 369 240 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6065 7777 96 Size: 274 80 33 51 1443 4088 1712 138 Fragments arranged by size: 4088 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4854 4886 5057 5089 7779 7811 4854 Size: 32 171 32 2690 32 4862 Fragments arranged by size: 4862 2690 171 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2462 1316 Size: 1146 6673 XbaI T'CTAG_A Cuts at: 5155 5155 Size: 7819 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2764 2987 3388 2764 Size: 223 401 7195 Fragments arranged by size: 7195 401 223 XhoI C'TCGA_G Cuts at: 1943 3958 1943 Size: 2015 5804 XmaI C'CCGG_G Cuts at: 1870 5144 1870 Size: 3274 4545 XmnI GAAnn'nnTTC Cuts at: 5948 5948 Size: 7819 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5627 276 Size: 53 83 186 936 4093 2468 Fragments arranged by size: 4093 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacII SexAI SrfI SwaI