(Circular) (Six-base) MAPSORT of: pFN21ASDA1859.seq Check: 949 from: 1 to: 6952 pFN21ASDA1859 6952 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2738 2738 Size: 6952 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4762 278 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4295 1937 Size: 2358 4594 Acc65I G'GTAC_C Cuts at: 4273 4273 Size: 6952 AclI AA'CG_TT Cuts at: 1563 5079 5452 1563 Size: 3516 373 3063 Fragments arranged by size: 3516 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2023 2389 3362 3452 5015 6399 6667 1632 Size: 391 366 973 90 1563 1384 268 1917 Fragments arranged by size: 1917 1563 1384 973 391 366 268 90 AfeI AGC'GCT Cuts at: 3948 3948 Size: 6952 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6755 AflIII A'CryG_T Cuts at: 1203 5851 6828 1203 Size: 4648 977 1327 Fragments arranged by size: 4648 1327 977 AgeI A'CCGG_T Cuts at: 5783 5783 Size: 6952 AhdI GACnn_n'nnGTC Cuts at: 5681 5681 Size: 6952 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6952 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6952 AlwNI CAG_nnn'CTG Cuts at: 2275 2951 2954 3005 4695 6267 2275 Size: 676 3 51 1690 1572 2960 Fragments arranged by size: 2960 1690 1572 676 51 3 ApaI G_GGCC'C Cuts at: 2499 2636 2819 3913 3925 2499 Size: 137 183 1094 12 5526 Fragments arranged by size: 5526 1094 183 137 12 ApaLI G'TGCA_C Cuts at: 5009 6165 5009 Size: 1156 5796 ApoI r'AATT_y Cuts at: 1493 2135 3740 4261 4487 4639 6748 1493 Size: 642 1605 521 226 152 2109 1697 Fragments arranged by size: 2109 1697 1605 642 521 226 152 AseI AT'TA_AT Cuts at: 160 1032 5506 160 Size: 872 4474 1606 Fragments arranged by size: 4474 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 AvaI C'yCGr_G Cuts at: 1870 1943 2373 2567 3199 3825 4277 1870 Size: 73 430 194 632 626 452 4545 Fragments arranged by size: 4545 632 626 452 430 194 73 BamHI G'GATC_C Cuts at: 2296 3423 4035 4233 4282 4649 2296 Size: 1127 612 198 49 367 4599 Fragments arranged by size: 4599 1127 612 367 198 49 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 3636 4130 4214 4273 Size: 325 217 627 372 1477 494 84 59 Cuts at: 4273 5728 618 Size: 1455 1842 Fragments arranged by size: 1842 1477 1455 627 494 372 325 217 84 59 BanII G_rGCy'C Cuts at: 1391 2241 2499 2636 2819 3033 3172 3763 3913 Size: 850 258 137 183 214 139 591 150 Cuts at: 3913 3925 3990 4271 1391 Size: 12 65 281 4072 Fragments arranged by size: 4072 850 591 281 258 214 183 150 139 137 65 12 BbeI G_GCGC'C Cuts at: 4218 4218 Size: 6952 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2076 2719 928 Size: 971 177 643 5161 Fragments arranged by size: 5161 971 643 177 BbvCI CC'TCA_GC Cuts at: 2028 3487 2028 Size: 1459 5493 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3884 3918 5143 5177 1903 Size: 34 1947 34 1225 34 3678 Fragments arranged by size: 3678 1947 1225 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2452 4844 6054 2452 Size: 2392 1210 3350 Fragments arranged by size: 3350 2392 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6952 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2486 2570 2618 Size: 107 122 71 631 605 814 84 48 Cuts at: 2618 2801 2969 3584 5563 136 Size: 183 168 615 1979 1525 Fragments arranged by size: 1979 1525 814 631 615 605 183 168 122 107 84 71 48 BglII A'GATC_T Cuts at: 6947 6947 Size: 6952 BlpI GC'TnA_GC Cuts at: 3015 3027 4369 3015 Size: 12 1342 5598 Fragments arranged by size: 5598 1342 12 Bme1580I G_kGCm'C Cuts at: 1790 2499 2636 2819 2841 3913 3925 5013 6169 Size: 709 137 183 22 1072 12 1088 1156 Cuts at: 6169 1790 Size: 2573 Fragments arranged by size: 2573 1156 1088 1072 709 183 137 22 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6952 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2814 4683 5641 Size: 416 53 83 399 24 1397 1869 958 Cuts at: 5641 442 Size: 1753 Fragments arranged by size: 1869 1753 1397 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2424 1056 Size: 1368 5584 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3132 3132 Size: 6952 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3157 5612 1239 Size: 1918 2455 2579 Fragments arranged by size: 2579 2455 1918 Bpu10I CC'TnA_GC Cuts at: 2028 2490 2505 2985 3487 2028 Size: 462 15 480 502 5493 Fragments arranged by size: 5493 502 480 462 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3202 5077 5942 6240 3202 Size: 1875 865 298 3914 Fragments arranged by size: 3914 1875 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2452 2776 3764 3813 5615 882 Size: 617 953 324 988 49 1802 2219 Fragments arranged by size: 2219 1802 988 953 617 324 49 BsaAI yAC'GTr Cuts at: 493 1204 3226 4107 4249 4743 493 Size: 711 2022 881 142 494 2702 Fragments arranged by size: 2702 2022 881 711 494 142 BsaBI GATnn'nnATC Cuts at: 3320 3932 6946 3320 Size: 612 3014 3326 Fragments arranged by size: 3326 3014 612 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2685 3552 4119 4215 Size: 53 83 186 936 1152 867 567 96 Cuts at: 4215 4759 5141 5776 275 Size: 544 382 635 1451 Fragments arranged by size: 1451 1152 936 867 635 567 544 382 186 96 83 53 BsaWI w'CCGG_w Cuts at: 2299 4188 5385 5783 6057 6204 6615 2299 Size: 1889 1197 398 274 147 411 2636 Fragments arranged by size: 2636 1889 1197 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6727 Fragments arranged by size: 6727 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3116 3119 3475 3524 1186 Size: 1930 3 356 49 4614 Fragments arranged by size: 4614 1930 356 49 3 BseYI C'CCAG_C Cuts at: 2463 2696 2791 3966 4169 6155 6651 2463 Size: 233 95 1175 203 1986 496 2764 Fragments arranged by size: 2764 1986 1175 496 233 203 95 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3018 3457 1163 Size: 573 1282 439 4658 Fragments arranged by size: 4658 1282 573 439 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2325 4402 5163 6191 1540 Size: 275 186 324 2077 761 1028 2301 Fragments arranged by size: 2301 2077 1028 761 324 275 186 BsiHKAI G_wGCw'C Cuts at: 3990 5013 5098 6169 3990 Size: 1023 85 1071 4773 Fragments arranged by size: 4773 1071 1023 85 BsmI GAATG_Cn' Cuts at: 4463 4556 4463 Size: 93 6859 BsmBI CGTCTCn'nnnn_ Cuts at: 2312 2312 Size: 6952 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2241 2499 2636 2819 2841 3033 3172 Size: 399 451 258 137 183 22 192 139 Cuts at: 3172 3763 3913 3925 3990 4271 5013 5098 6169 Size: 591 150 12 65 281 742 85 1071 Cuts at: 6169 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 591 451 399 281 258 192 183 150 139 137 85 65 22 12 BspEI T'CCGG_A Cuts at: 2299 4188 6615 2299 Size: 1889 2427 2636 Fragments arranged by size: 2636 2427 1889 BspHI T'CATG_A Cuts at: 3561 4841 6571 3561 Size: 1280 1730 3942 Fragments arranged by size: 3942 1730 1280 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2434 2738 2794 4307 844 Size: 1590 304 56 1513 3489 Fragments arranged by size: 3489 1590 1513 304 56 BsrBI CCG'CTC Cuts at: 1394 1823 4839 1394 Size: 429 3016 3507 Fragments arranged by size: 3507 3016 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2285 5447 5621 65 Size: 1183 1037 3162 174 1396 Fragments arranged by size: 3162 1396 1183 1037 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2691 2743 5596 5783 1700 Size: 110 881 52 2853 187 2869 Fragments arranged by size: 2869 2853 881 187 110 52 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6952 BssHII G'CGCG_C Cuts at: 1925 3328 1925 Size: 1403 5549 BssSI C'ACGA_G Cuts at: 2974 5012 6024 2974 Size: 2038 1012 3902 Fragments arranged by size: 3902 2038 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2951 2954 3005 794 Size: 2157 3 51 4741 Fragments arranged by size: 4741 2157 51 3 BstBI TT'CG_AA Cuts at: 2007 5756 2007 Size: 3749 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6952 BstXI CCAn_nnnn'nTGG Cuts at: 2557 2680 3076 3135 2557 Size: 123 396 59 6374 Fragments arranged by size: 6374 396 123 59 BstYI r'GATC_y Cuts at: 1972 2296 3315 3423 3711 4035 4233 4282 4649 Size: 324 1019 108 288 324 198 49 367 Cuts at: 4649 5034 5051 6503 6589 6601 6947 1972 Size: 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1019 385 367 346 324 324 288 198 108 86 49 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3120 513 Size: 552 957 1098 4345 Fragments arranged by size: 4345 1098 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3784 4035 487 Size: 683 56 2558 251 3404 Fragments arranged by size: 3404 2558 683 251 56 BtsI GCAGTG_nn' Cuts at: 3009 4132 4468 5262 5282 3009 Size: 1123 336 794 20 4679 Fragments arranged by size: 4679 1123 794 336 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6952 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3864 3899 529 Size: 35 3300 35 3582 Fragments arranged by size: 3582 3300 35 35 DraI TTT'AAA Cuts at: 4256 4256 Size: 6952 DraIII CAC_nnn'GTG Cuts at: 2431 2431 Size: 6952 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2890 3064 3980 5959 809 Size: 507 1574 174 916 1979 1802 Fragments arranged by size: 1979 1802 1574 916 507 174 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2322 2433 2907 Size: 54 1425 50 275 18 492 111 474 Cuts at: 2907 2970 3891 4399 5288 6917 8 Size: 63 921 508 889 1629 43 Fragments arranged by size: 1629 1425 921 889 508 492 474 275 111 63 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2322 4399 1537 Size: 275 510 2077 4090 Fragments arranged by size: 4090 2077 510 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2100 4882 1353 Size: 747 2782 3423 Fragments arranged by size: 3423 2782 747 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5539 5913 6059 387 Size: 1064 138 3950 374 146 1280 Fragments arranged by size: 3950 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3988 3988 Size: 6952 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2023 2389 3157 3362 3452 5015 5612 Size: 393 391 366 768 205 90 1563 597 Cuts at: 5612 6399 6667 1239 Size: 787 268 1524 Fragments arranged by size: 1563 1524 787 768 597 393 391 366 268 205 90 EcoNI CCTnn'n_nnAGG Cuts at: 2279 3037 3260 3596 4030 2279 Size: 758 223 336 434 5201 Fragments arranged by size: 5201 758 434 336 223 EcoO109I rG'GnC_Cy Cuts at: 2495 2632 2633 2815 2816 2849 3204 3751 3787 Size: 137 1 182 1 33 355 547 36 Cuts at: 3787 3909 3921 2495 Size: 122 12 5526 Fragments arranged by size: 5526 547 355 182 137 122 36 33 12 1 1 EcoRI G'AATT_C Cuts at: 2135 3740 4261 4639 2135 Size: 1605 521 378 4448 Fragments arranged by size: 4448 1605 521 378 FspI TGC'GCA Cuts at: 5458 5458 Size: 6952 HaeII r_GCGC'y Cuts at: 1678 2646 2906 3950 4218 6099 1678 Size: 968 260 1044 268 1881 2531 Fragments arranged by size: 2531 1881 1044 968 268 260 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4104 4136 5593 5625 5667 Size: 32 135 32 2532 32 1457 32 42 Cuts at: 5667 5699 1373 Size: 32 2626 Fragments arranged by size: 2626 2532 1457 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4296 4542 669 Size: 988 281 2358 246 3079 Fragments arranged by size: 3079 2358 988 281 246 HindIII A'AGCT_T Cuts at: 748 2035 2203 748 Size: 1287 168 5497 Fragments arranged by size: 5497 1287 168 HpaI GTT'AAC Cuts at: 4542 4542 Size: 6952 KasI G'GCGC_C Cuts at: 4214 4214 Size: 6952 KpnI G_GTAC'C Cuts at: 4277 4277 Size: 6952 MfeI C'AATT_G Cuts at: 4551 4551 Size: 6952 MluI A'CGCG_T Cuts at: 6828 6828 Size: 6952 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2385 6066 6250 1305 Size: 1080 3681 184 2007 Fragments arranged by size: 3681 2007 1080 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2435 2909 2972 10 Size: 54 1425 343 603 474 63 3990 Fragments arranged by size: 3990 1425 603 474 343 63 54 MslI CAynn'nnrTG Cuts at: 518 1166 3078 3560 4910 5269 5428 518 Size: 648 1912 482 1350 359 159 2042 Fragments arranged by size: 2042 1912 1350 648 482 359 159 MspA1I CmG'CkG Cuts at: 1606 2293 2381 2390 2951 3002 3122 4368 5045 Size: 687 88 9 561 51 120 1246 677 Cuts at: 5045 6193 6438 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1246 1148 687 677 561 245 120 88 51 9 NaeI GCC'GGC Cuts at: 2745 2745 Size: 6952 NarI GG'CG_CC Cuts at: 4215 4215 Size: 6952 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5443 Fragments arranged by size: 5443 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6952 NgoMIV G'CCGG_C Cuts at: 2743 2743 Size: 6952 NheI G'CTAG_C Cuts at: 1052 2420 1052 Size: 1368 5584 NotI GC'GGCC_GC Cuts at: 4399 4399 Size: 6952 NspI r_CATG'y Cuts at: 1132 1229 3340 3905 4310 5855 1132 Size: 97 2111 565 405 1545 2229 Fragments arranged by size: 2229 2111 1545 565 405 97 PasI CC'CwG_GG Cuts at: 1348 2241 3033 3626 1348 Size: 893 792 593 4674 Fragments arranged by size: 4674 893 792 593 PciI A'CATG_T Cuts at: 5851 5851 Size: 6952 PflMI CCAn_nnn'nTGG Cuts at: 1108 2275 2431 1108 Size: 1167 156 5629 Fragments arranged by size: 5629 1167 156 PfoI T'CCnGG_A Cuts at: 4229 4229 Size: 6952 PmeI GTTT'AAAC Cuts at: 4256 4256 Size: 6952 PmlI CAC'GTG Cuts at: 4107 4107 Size: 6952 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5021 5053 6534 6566 5021 Size: 32 1481 32 5407 Fragments arranged by size: 5407 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2849 3204 3787 2849 Size: 355 583 6014 Fragments arranged by size: 6014 583 355 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6952 PsiI TTA'TAA Cuts at: 4522 4522 Size: 6952 PspOMI G'GGCC_C Cuts at: 2495 2632 2815 3909 3921 2495 Size: 137 183 1094 12 5526 Fragments arranged by size: 5526 1094 183 137 12 PstI C_TGCA'G Cuts at: 830 3264 4304 830 Size: 2434 1040 3478 Fragments arranged by size: 3478 2434 1040 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6952 PvuII CAG'CTG Cuts at: 2293 2951 3002 2293 Size: 658 51 6243 Fragments arranged by size: 6243 658 51 SacI G_AGCT'C Cuts at: 3990 3990 Size: 6952 SacII CC_GC'GG Cuts at: 3123 3123 Size: 6952 SalI G'TCGA_C Cuts at: 1936 4294 1936 Size: 2358 4594 SbfI CC_TGCA'GG Cuts at: 4304 4304 Size: 6952 ScaI AGT'ACT Cuts at: 5200 6912 5200 Size: 1712 5240 SfcI C'TryA_G Cuts at: 826 1045 2059 3260 4300 5435 6116 6307 826 Size: 219 1014 1201 1040 1135 681 191 1471 Fragments arranged by size: 1471 1201 1135 1040 1014 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2969 2969 Size: 6952 SfoI GGC'GCC Cuts at: 4216 4216 Size: 6952 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6952 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6952 SmaI CCC'GGG Cuts at: 1872 3827 4279 1872 Size: 1955 452 4545 Fragments arranged by size: 4545 1955 452 SmlI C'TyrA_G Cuts at: 820 1017 1943 3217 5056 5957 6219 820 Size: 197 926 1274 1839 901 262 1553 Fragments arranged by size: 1839 1553 1274 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4249 4743 493 Size: 3756 494 2702 Fragments arranged by size: 3756 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6952 SphI G_CATG'C Cuts at: 1132 4310 1132 Size: 3178 3774 SspI AAT'ATT Cuts at: 5 52 4876 5 Size: 47 4824 2081 Fragments arranged by size: 4824 2081 47 StuI AGG'CCT Cuts at: 1517 2217 1517 Size: 700 6252 StyI C'CwwG_G Cuts at: 513 1065 2022 2958 3499 3510 4391 513 Size: 552 957 936 541 11 881 3074 Fragments arranged by size: 3074 957 936 881 552 541 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2911 3145 3654 4979 4996 5149 5334 Size: 1255 1331 234 509 1325 17 153 185 Cuts at: 5334 5779 325 Size: 445 1498 Fragments arranged by size: 1498 1331 1325 1255 509 445 234 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4068 5198 6910 Size: 274 80 33 51 1443 2091 1130 1712 Cuts at: 6910 96 Size: 138 Fragments arranged by size: 2091 1712 1443 1130 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6912 6944 6912 Size: 32 6920 Tth111I GACn'n_nGTC Cuts at: 1316 2093 1316 Size: 777 6175 XbaI T'CTAG_A Cuts at: 4288 4288 Size: 6952 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2849 2965 2849 Size: 116 6836 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6952 XmaI C'CCGG_G Cuts at: 1870 3825 4277 1870 Size: 1955 452 4545 Fragments arranged by size: 4545 1955 452 XmnI GAAnn'nnTTC Cuts at: 5081 5081 Size: 6952 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4760 276 Size: 53 83 186 936 3226 2468 Fragments arranged by size: 3226 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SapI SexAI SrfI SwaI