(Circular) (Six-base) MAPSORT of: pFN21ASDA1835.seq Check: 3365 from: 1 to: 6490 pFN21ASDA1835 6490 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2806 2806 Size: 6490 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3324 4300 278 Size: 53 83 186 936 1788 976 2468 Fragments arranged by size: 2468 1788 976 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3783 3833 1937 Size: 1846 50 4594 Fragments arranged by size: 4594 1846 50 Acc65I G'GTAC_C Cuts at: 3811 3811 Size: 6490 AclI AA'CG_TT Cuts at: 1563 4617 4990 1563 Size: 3054 373 3063 Fragments arranged by size: 3063 3054 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2588 3029 4553 5937 6205 1632 Size: 956 441 1524 1384 268 1917 Fragments arranged by size: 1917 1524 1384 956 441 268 AfeI AGC'GCT Cuts at: 2264 2264 Size: 6490 AflII C'TTAA_G Cuts at: 820 1017 2377 820 Size: 197 1360 4933 Fragments arranged by size: 4933 1360 197 AflIII A'CryG_T Cuts at: 1203 2280 3408 5389 6366 1203 Size: 1077 1128 1981 977 1327 Fragments arranged by size: 1981 1327 1128 1077 977 AgeI A'CCGG_T Cuts at: 5321 5321 Size: 6490 AhdI GACnn_n'nnGTC Cuts at: 2526 5219 2526 Size: 2693 3797 AleI CACnn'nnGTG Cuts at: 1166 2274 1166 Size: 1108 5382 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6490 AlwNI CAG_nnn'CTG Cuts at: 3772 4233 5805 3772 Size: 461 1572 4457 Fragments arranged by size: 4457 1572 461 ApaI G_GGCC'C Cuts at: 2400 3375 2400 Size: 975 5515 ApaLI G'TGCA_C Cuts at: 3575 4547 5703 3575 Size: 972 1156 4362 Fragments arranged by size: 4362 1156 972 ApoI r'AATT_y Cuts at: 1493 3799 4025 4177 6286 1493 Size: 2306 226 152 2109 1697 Fragments arranged by size: 2306 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2829 5044 160 Size: 872 1797 2215 1606 Fragments arranged by size: 2215 1797 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 AvaI C'yCGr_G Cuts at: 1870 1943 3419 3815 1870 Size: 73 1476 396 4545 Fragments arranged by size: 4545 1476 396 73 AvrII C'CTAG_G Cuts at: 3367 3367 Size: 6490 BamHI G'GATC_C Cuts at: 3820 4187 3820 Size: 367 6123 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2609 2777 3811 5266 618 Size: 325 217 627 822 168 1034 1455 1842 Fragments arranged by size: 1842 1455 1034 822 627 325 217 168 BanII G_rGCy'C Cuts at: 1391 2359 2400 2438 3375 3383 3809 1391 Size: 968 41 38 937 8 426 4072 Fragments arranged by size: 4072 968 937 426 41 38 8 BbeI G_GCGC'C Cuts at: 2613 2781 2613 Size: 168 6322 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2040 2166 2810 3606 928 Size: 971 141 126 644 796 3812 Fragments arranged by size: 3812 971 796 644 141 126 BbvCI CC'TCA_GC Cuts at: 2964 3216 2964 Size: 252 6238 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4681 4715 1903 Size: 34 2744 34 3678 Fragments arranged by size: 3678 2744 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4382 5592 4382 Size: 1210 5280 BclI T'GATC_A Cuts at: 1548 1548 Size: 6490 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2048 5101 136 Size: 107 122 71 631 605 376 3053 1525 Fragments arranged by size: 3053 1525 631 605 376 122 107 71 BglII A'GATC_T Cuts at: 6485 6485 Size: 6490 BlpI GC'TnA_GC Cuts at: 2358 2437 2985 3907 2358 Size: 79 548 922 4941 Fragments arranged by size: 4941 922 548 79 Bme1580I G_kGCm'C Cuts at: 1790 2400 2548 3375 3579 4551 5707 1790 Size: 610 148 827 204 972 1156 2573 Fragments arranged by size: 2573 1156 972 827 610 204 148 BmgBI CAC'GTC Cuts at: 1318 3411 1318 Size: 2093 4397 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3288 4221 5179 Size: 416 53 83 399 24 1871 933 958 Cuts at: 5179 442 Size: 1753 Fragments arranged by size: 1871 1753 958 933 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6490 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2293 2618 2672 3011 3077 3333 5150 1239 Size: 1054 325 54 339 66 256 1817 2579 Fragments arranged by size: 2579 1817 1054 339 325 256 66 54 Bpu10I CC'TnA_GC Cuts at: 2964 3216 2964 Size: 252 6238 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2414 2447 3575 4615 5480 5778 2414 Size: 33 1128 1040 865 298 3126 Fragments arranged by size: 3126 1128 1040 865 298 33 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2789 3436 5153 882 Size: 617 1290 647 1717 2219 Fragments arranged by size: 2219 1717 1290 647 617 BsaAI yAC'GTr Cuts at: 493 1204 3787 4281 493 Size: 711 2583 494 2702 Fragments arranged by size: 2702 2583 711 494 BsaBI GATnn'nnATC Cuts at: 6484 6484 Size: 6490 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2610 2778 3086 3321 Size: 53 83 186 936 1077 168 308 235 Cuts at: 3321 4297 4679 5314 275 Size: 976 382 635 1451 Fragments arranged by size: 1451 1077 976 936 635 382 308 235 186 168 83 53 BsaWI w'CCGG_w Cuts at: 2532 4923 5321 5595 5742 6153 2532 Size: 2391 398 274 147 411 2869 Fragments arranged by size: 2869 2391 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6265 Fragments arranged by size: 6265 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2669 3125 3128 3131 3134 3173 3176 3194 Size: 1483 456 3 3 3 39 3 18 Cuts at: 3194 3206 3209 3212 3215 3523 3560 1186 Size: 12 3 3 3 308 37 4116 Fragments arranged by size: 4116 1483 456 308 39 37 18 12 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2351 3396 5693 6189 2351 Size: 1045 2297 496 2652 Fragments arranged by size: 2652 2297 1045 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2883 3512 3680 1163 Size: 573 1147 629 168 3973 Fragments arranged by size: 3973 1147 629 573 168 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3940 4701 5729 1540 Size: 275 186 1939 761 1028 2301 Fragments arranged by size: 2301 1939 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2438 3383 3579 4551 4636 5707 2438 Size: 945 196 972 85 1071 3221 Fragments arranged by size: 3221 1071 972 945 196 85 BsmI GAATG_Cn' Cuts at: 2390 4001 4094 2390 Size: 1611 93 4786 Fragments arranged by size: 4786 1611 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3328 3328 Size: 6490 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2359 2400 2438 2548 3375 3383 3579 Size: 399 569 41 38 110 827 8 196 Cuts at: 3579 3809 4551 4636 5707 1391 Size: 230 742 85 1071 2174 Fragments arranged by size: 2174 1071 827 742 569 399 230 196 110 85 41 38 8 BspEI T'CCGG_A Cuts at: 6153 6153 Size: 6490 BspHI T'CATG_A Cuts at: 4379 6109 4379 Size: 1730 4760 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2806 3845 844 Size: 1962 1039 3489 Fragments arranged by size: 3489 1962 1039 BsrBI CCG'CTC Cuts at: 1394 1823 4377 1394 Size: 429 2554 3507 Fragments arranged by size: 3507 2554 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2037 2871 3615 4985 5159 65 Size: 1183 789 834 744 1370 174 1396 Fragments arranged by size: 1396 1370 1183 834 789 744 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5134 5321 1700 Size: 110 3324 187 2869 Fragments arranged by size: 3324 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3774 96 Size: 3678 2812 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6490 BssSI C'ACGA_G Cuts at: 3572 4550 5562 3572 Size: 978 1012 4500 Fragments arranged by size: 4500 1012 978 BstAPI GCAn_nnn'nTGC Cuts at: 794 2132 3506 794 Size: 1338 1374 3778 Fragments arranged by size: 3778 1374 1338 BstBI TT'CG_AA Cuts at: 2007 5294 2007 Size: 3287 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6490 BstXI CCAn_nnnn'nTGG Cuts at: 2620 3082 3334 2620 Size: 462 252 5776 Fragments arranged by size: 5776 462 252 BstYI r'GATC_y Cuts at: 1972 2307 2502 3820 4187 4572 4589 6041 6127 Size: 335 195 1318 367 385 17 1452 86 Cuts at: 6127 6139 6485 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1318 385 367 346 335 195 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2781 2787 2887 2970 513 Size: 552 957 759 6 100 83 4033 Fragments arranged by size: 4033 957 759 552 100 83 6 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2212 487 Size: 683 56 986 4765 Fragments arranged by size: 4765 986 683 56 BtsI GCAGTG_nn' Cuts at: 2121 2613 2697 3495 4006 4800 4820 2121 Size: 492 84 798 511 794 20 3791 Fragments arranged by size: 3791 798 794 511 492 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6490 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2458 2493 529 Size: 35 1894 35 4526 Fragments arranged by size: 4526 1894 35 35 DraI TTT'AAA Cuts at: 3794 3794 Size: 6490 DraIII CAC_nnn'GTG Cuts at: 3658 3658 Size: 6490 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3409 5497 809 Size: 507 2093 2088 1802 Fragments arranged by size: 2093 2088 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2784 2790 Size: 54 1425 50 275 18 246 708 6 Cuts at: 2790 3072 3937 4826 6455 8 Size: 282 865 889 1629 43 Fragments arranged by size: 1629 1425 889 865 708 282 275 246 54 50 43 18 6 EagI C'GGCC_G Cuts at: 1537 1812 3937 1537 Size: 275 2125 4090 Fragments arranged by size: 4090 2125 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3126 3135 3150 3171 3183 3204 4420 1353 Size: 1773 9 15 21 12 21 1216 3423 Fragments arranged by size: 3423 1773 1216 21 21 15 12 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5077 5451 5597 387 Size: 1064 138 3488 374 146 1280 Fragments arranged by size: 3488 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2436 3381 2436 Size: 945 5545 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2293 2588 2618 2672 3011 3029 3077 Size: 393 661 295 30 54 339 18 48 Cuts at: 3077 3333 4553 5150 5937 6205 1239 Size: 256 1220 597 787 268 1524 Fragments arranged by size: 1524 1220 787 661 597 393 339 295 268 256 54 48 30 18 EcoNI CCTnn'n_nnAGG Cuts at: 2654 2708 2654 Size: 54 6436 EcoO109I rG'GnC_Cy Cuts at: 2422 2646 3371 3682 2422 Size: 224 725 311 5230 Fragments arranged by size: 5230 725 311 224 EcoRI G'AATT_C Cuts at: 3799 4177 3799 Size: 378 6112 FspI TGC'GCA Cuts at: 4996 4996 Size: 6490 HaeII r_GCGC'y Cuts at: 1678 2266 2613 2781 3647 3729 5637 1678 Size: 588 347 168 866 82 1908 2531 Fragments arranged by size: 2531 1908 866 588 347 168 82 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2234 2266 2512 2544 5131 Size: 32 135 32 662 32 246 32 2587 Cuts at: 5131 5163 5205 5237 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2587 662 246 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2725 3834 4080 669 Size: 988 281 787 1109 246 3079 Fragments arranged by size: 3079 1109 988 787 281 246 HindIII A'AGCT_T Cuts at: 748 2628 748 Size: 1880 4610 HpaI GTT'AAC Cuts at: 4080 4080 Size: 6490 KasI G'GCGC_C Cuts at: 2609 2777 2609 Size: 168 6322 KpnI G_GTAC'C Cuts at: 3815 3815 Size: 6490 MfeI C'AATT_G Cuts at: 4089 4089 Size: 6490 MluI A'CGCG_T Cuts at: 6366 6366 Size: 6490 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2232 3573 5604 5788 1305 Size: 927 1341 2031 184 2007 Fragments arranged by size: 2031 2007 1341 927 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2786 3074 10 Size: 54 1425 343 954 288 3426 Fragments arranged by size: 3426 1425 954 343 288 54 MslI CAynn'nnrTG Cuts at: 518 1166 2274 4448 4807 4966 518 Size: 648 1108 2174 359 159 2042 Fragments arranged by size: 2174 2042 1108 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2089 2351 2362 2889 3906 4583 5731 5976 Size: 483 262 11 527 1017 677 1148 245 Cuts at: 5976 1606 Size: 2120 Fragments arranged by size: 2120 1148 1017 677 527 483 262 245 11 NarI GG'CG_CC Cuts at: 2610 2778 2610 Size: 168 6322 NcoI C'CATG_G Cuts at: 513 1065 2022 2787 2970 513 Size: 552 957 765 183 4033 Fragments arranged by size: 4033 957 765 552 183 NdeI CA'TA_TG Cuts at: 387 387 Size: 6490 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6490 NotI GC'GGCC_GC Cuts at: 3937 3937 Size: 6490 NspI r_CATG'y Cuts at: 1132 1229 2284 2668 3848 5393 1132 Size: 97 1055 384 1180 1545 2229 Fragments arranged by size: 2229 1545 1180 1055 384 97 PasI CC'CwG_GG Cuts at: 1348 3019 1348 Size: 1671 4819 PciI A'CATG_T Cuts at: 2280 5389 2280 Size: 3109 3381 PflMI CCAn_nnn'nTGG Cuts at: 1108 2675 1108 Size: 1567 4923 PmeI GTTT'AAAC Cuts at: 3794 3794 Size: 6490 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2451 2483 4559 4591 6072 6104 2451 Size: 32 2076 32 1481 32 2837 Fragments arranged by size: 2837 2076 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2646 2646 Size: 6490 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6490 PsiI TTA'TAA Cuts at: 4060 4060 Size: 6490 PspOMI G'GGCC_C Cuts at: 2396 3371 2396 Size: 975 5515 PstI C_TGCA'G Cuts at: 830 2128 2457 2515 3319 3768 3842 830 Size: 1298 329 58 804 449 74 3478 Fragments arranged by size: 3478 1298 804 449 329 74 58 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6490 PvuII CAG'CTG Cuts at: 2089 2351 2362 2089 Size: 262 11 6217 Fragments arranged by size: 6217 262 11 RsrII CG'GwC_CG Cuts at: 2301 2301 Size: 6490 SacI G_AGCT'C Cuts at: 2438 3383 2438 Size: 945 5545 SacII CC_GC'GG Cuts at: 2890 2890 Size: 6490 SalI G'TCGA_C Cuts at: 1936 3832 1936 Size: 1896 4594 SanDI GG'GwC_CC Cuts at: 2646 2646 Size: 6490 SapI GCTCTTCn'nnn_ Cuts at: 3204 3204 Size: 6490 SbfI CC_TGCA'GG Cuts at: 2457 3842 2457 Size: 1385 5105 ScaI AGT'ACT Cuts at: 4738 6450 4738 Size: 1712 4778 SfcI C'TryA_G Cuts at: 826 1045 2124 2453 2511 3315 3764 3838 4973 Size: 219 1079 329 58 804 449 74 1135 Cuts at: 4973 5654 5845 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1079 804 681 449 329 219 191 74 58 SfoI GGC'GCC Cuts at: 2611 2779 2611 Size: 168 6322 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6490 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6490 SmaI CCC'GGG Cuts at: 1872 3817 1872 Size: 1945 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2377 2429 2462 3590 4594 5495 Size: 197 926 434 52 33 1128 1004 901 Cuts at: 5495 5757 820 Size: 262 1553 Fragments arranged by size: 1553 1128 1004 926 901 434 262 197 52 33 SnaBI TAC'GTA Cuts at: 493 3787 4281 493 Size: 3294 494 2702 Fragments arranged by size: 3294 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6490 SphI G_CATG'C Cuts at: 1132 3848 1132 Size: 2716 3774 SspI AAT'ATT Cuts at: 5 52 4414 5 Size: 47 4362 2081 Fragments arranged by size: 4362 2081 47 StuI AGG'CCT Cuts at: 1517 2237 1517 Size: 720 5770 StyI C'CwwG_G Cuts at: 513 1065 2022 2232 2548 2619 2787 2856 2970 Size: 552 957 210 316 71 168 69 114 Cuts at: 2970 3075 3367 3678 3929 513 Size: 105 292 311 251 3074 Fragments arranged by size: 3074 957 552 316 311 292 251 210 168 114 105 71 69 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2318 2537 4517 4534 4687 4872 5317 Size: 1255 738 219 1980 17 153 185 445 Cuts at: 5317 325 Size: 1498 Fragments arranged by size: 1980 1498 1255 738 445 219 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3774 4736 6448 Size: 274 80 33 51 1443 1797 962 1712 Cuts at: 6448 96 Size: 138 Fragments arranged by size: 1797 1712 1443 962 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2522 2527 2554 2559 6450 6482 2522 Size: 5 27 5 3891 32 2530 Fragments arranged by size: 3891 2530 32 27 5 5 Tth111I GACn'n_nGTC Cuts at: 1316 3605 1316 Size: 2289 4201 XbaI T'CTAG_A Cuts at: 3826 3826 Size: 6490 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2071 3727 2071 Size: 1656 4834 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6490 XmaI C'CCGG_G Cuts at: 1870 3815 1870 Size: 1945 4545 XmnI GAAnn'nnTTC Cuts at: 3042 3524 4619 3042 Size: 482 1095 4913 Fragments arranged by size: 4913 1095 482 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3322 4298 276 Size: 53 83 186 936 1788 976 2468 Fragments arranged by size: 2468 1788 976 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI BaeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI SexAI SfiI SrfI SwaI