(Circular) (Six-base) MAPSORT of: pFN21ASDA1785.seq Check: 915 from: 1 to: 6580 pFN21ASDA1785 6580 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4390 278 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2790 3923 1937 Size: 853 1133 4594 Fragments arranged by size: 4594 1133 853 Acc65I G'GTAC_C Cuts at: 3519 3901 3519 Size: 382 6198 AclI AA'CG_TT Cuts at: 1563 4707 5080 1563 Size: 3144 373 3063 Fragments arranged by size: 3144 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2330 3454 4643 6027 6295 1632 Size: 698 1124 1189 1384 268 1917 Fragments arranged by size: 1917 1384 1189 1124 698 268 AflII C'TTAA_G Cuts at: 820 1017 3440 820 Size: 197 2423 3960 Fragments arranged by size: 3960 2423 197 AflIII A'CryG_T Cuts at: 1203 3506 5479 6456 1203 Size: 2303 1973 977 1327 Fragments arranged by size: 2303 1973 1327 977 AgeI A'CCGG_T Cuts at: 5411 5411 Size: 6580 AhdI GACnn_n'nnGTC Cuts at: 5309 5309 Size: 6580 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6580 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2232 2604 3642 3804 1184 Size: 1048 372 1038 162 3960 Fragments arranged by size: 3960 1048 1038 372 162 AlwNI CAG_nnn'CTG Cuts at: 2597 3074 4323 5895 2597 Size: 477 1249 1572 3282 Fragments arranged by size: 3282 1572 1249 477 ApaLI G'TGCA_C Cuts at: 3675 4637 5793 3675 Size: 962 1156 4462 Fragments arranged by size: 4462 1156 962 ApoI r'AATT_y Cuts at: 1493 2195 3079 3533 3748 3889 4115 4267 6376 Size: 702 884 454 215 141 226 152 2109 Cuts at: 6376 1493 Size: 1697 Fragments arranged by size: 2109 1697 884 702 454 226 215 152 141 AseI AT'TA_AT Cuts at: 160 1032 2757 2993 5134 160 Size: 872 1725 236 2141 1606 Fragments arranged by size: 2141 1725 1606 872 236 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 AvaI C'yCGr_G Cuts at: 1870 1943 2055 3905 1870 Size: 73 112 1850 4545 Fragments arranged by size: 4545 1850 112 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3222 3255 3222 Size: 33 6547 BamHI G'GATC_C Cuts at: 3139 3910 4277 3139 Size: 771 367 5442 Fragments arranged by size: 5442 771 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2178 3519 3901 5356 618 Size: 325 217 627 391 1341 382 1455 1842 Fragments arranged by size: 1842 1455 1341 627 391 382 325 217 BanII G_rGCy'C Cuts at: 1391 2240 2275 3899 1391 Size: 849 35 1624 4072 Fragments arranged by size: 4072 1624 849 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5609 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4771 4805 1903 Size: 34 2834 34 3678 Fragments arranged by size: 3678 2834 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4472 5682 4472 Size: 1210 5370 BclI T'GATC_A Cuts at: 1548 1548 Size: 6580 BfrBI ATG'CAT Cuts at: 3093 3093 Size: 6580 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5191 136 Size: 107 122 71 631 605 3519 1525 Fragments arranged by size: 3519 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2803 6575 2803 Size: 3772 2808 BlpI GC'TnA_GC Cuts at: 3997 3997 Size: 6580 Bme1580I G_kGCm'C Cuts at: 1790 2181 3679 4641 5797 1790 Size: 391 1498 962 1156 2573 Fragments arranged by size: 2573 1498 1156 962 391 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6580 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4311 5269 442 Size: 416 53 83 399 24 2894 958 1753 Fragments arranged by size: 2894 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6580 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2134 2134 Size: 6580 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 5240 1239 Size: 4001 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4705 5570 5868 4705 Size: 865 298 5417 Fragments arranged by size: 5417 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5243 882 Size: 617 3744 2219 Fragments arranged by size: 3744 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2475 3877 4371 493 Size: 711 1271 1402 494 2702 Fragments arranged by size: 2702 1402 1271 711 494 BsaBI GATnn'nnATC Cuts at: 6574 6574 Size: 6580 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4387 4769 5404 275 Size: 53 83 186 936 2854 382 635 1451 Fragments arranged by size: 2854 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5013 5411 5685 5832 6243 5013 Size: 398 274 147 411 5350 Fragments arranged by size: 5350 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2331 2361 1178 Size: 30 165 30 928 30 5397 Fragments arranged by size: 5397 928 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2123 2367 1186 Size: 937 244 5399 Fragments arranged by size: 5399 937 244 BseYI C'CCAG_C Cuts at: 3226 5783 6279 3226 Size: 2557 496 3527 Fragments arranged by size: 3527 2557 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2610 2955 1163 Size: 573 874 345 4788 Fragments arranged by size: 4788 874 573 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4030 4791 5819 1540 Size: 275 186 2029 761 1028 2301 Fragments arranged by size: 2301 2029 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3679 4641 4726 5797 3679 Size: 962 85 1071 4462 Fragments arranged by size: 4462 1071 962 85 BsmI GAATG_Cn' Cuts at: 2985 4091 4184 2985 Size: 1106 93 5381 Fragments arranged by size: 5381 1106 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2181 2240 2275 3679 3899 4641 4726 Size: 399 391 59 35 1404 220 742 85 Cuts at: 4726 5797 1391 Size: 1071 2174 Fragments arranged by size: 2174 1404 1071 742 399 391 220 85 59 35 BspEI T'CCGG_A Cuts at: 6243 6243 Size: 6580 BspHI T'CATG_A Cuts at: 2613 3651 4469 6199 2613 Size: 1038 818 1730 2994 Fragments arranged by size: 2994 1730 1038 818 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3935 844 Size: 3091 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4467 1394 Size: 429 2644 3507 Fragments arranged by size: 3507 2644 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2216 2576 2844 3776 5075 5249 65 Size: 1183 968 360 268 932 1299 174 1396 Fragments arranged by size: 1396 1299 1183 968 932 360 268 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5224 5411 1700 Size: 110 3414 187 2869 Fragments arranged by size: 3414 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6580 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6580 BssSI C'ACGA_G Cuts at: 3795 4640 5652 3795 Size: 845 1012 4723 Fragments arranged by size: 4723 1012 845 BstAPI GCAn_nnn'nTGC Cuts at: 794 3267 794 Size: 2473 4107 BstBI TT'CG_AA Cuts at: 2007 5384 2007 Size: 3377 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6580 BstXI CCAn_nnnn'nTGG Cuts at: 2089 2089 Size: 6580 BstYI r'GATC_y Cuts at: 1972 2026 2803 3139 3910 4277 4662 4679 6131 Size: 54 777 336 771 367 385 17 1452 Cuts at: 6131 6217 6229 6575 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 777 771 385 367 346 336 86 54 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5071 Fragments arranged by size: 5071 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5841 Fragments arranged by size: 5841 683 56 BtsI GCAGTG_nn' Cuts at: 2221 3788 4096 4890 4910 2221 Size: 1567 308 794 20 3891 Fragments arranged by size: 3891 1567 794 308 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6580 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2691 2726 529 Size: 35 2127 35 4383 Fragments arranged by size: 4383 2127 35 35 DraI TTT'AAA Cuts at: 3884 3884 Size: 6580 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5587 809 Size: 507 4271 1802 Fragments arranged by size: 4271 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2163 2401 4027 Size: 54 1425 50 275 18 333 238 1626 Cuts at: 4027 4916 6545 8 Size: 889 1629 43 Fragments arranged by size: 1629 1626 1425 889 333 275 238 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4027 1537 Size: 275 2215 4090 Fragments arranged by size: 4090 2215 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2385 2934 3483 4510 1353 Size: 1032 549 549 1027 3423 Fragments arranged by size: 3423 1032 1027 549 549 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5167 5541 5687 387 Size: 1064 138 3578 374 146 1280 Fragments arranged by size: 3578 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2330 3454 4643 5240 6027 6295 1239 Size: 393 698 1124 1189 597 787 268 1524 Fragments arranged by size: 1524 1189 1124 787 698 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 3343 3343 Size: 6580 EcoRI G'AATT_C Cuts at: 2195 3889 4267 2195 Size: 1694 378 4508 Fragments arranged by size: 4508 1694 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3484 3484 Size: 6580 FspI TGC'GCA Cuts at: 5086 5086 Size: 6580 HaeII r_GCGC'y Cuts at: 1678 3060 5727 1678 Size: 1382 2667 2531 Fragments arranged by size: 2667 2531 1382 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2331 2363 3045 3077 5221 Size: 32 135 32 759 32 682 32 2144 Cuts at: 5221 5253 5295 5327 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2144 759 682 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2350 3924 4170 669 Size: 988 281 412 1574 246 3079 Fragments arranged by size: 3079 1574 988 412 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6580 HpaI GTT'AAC Cuts at: 4170 4170 Size: 6580 KpnI G_GTAC'C Cuts at: 3523 3905 3523 Size: 382 6198 MfeI C'AATT_G Cuts at: 4179 4179 Size: 6580 MluI A'CGCG_T Cuts at: 6456 6456 Size: 6580 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5694 5878 1305 Size: 4389 184 2007 Fragments arranged by size: 4389 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2403 10 Size: 54 1425 343 571 4187 Fragments arranged by size: 4187 1425 571 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2586 2618 3684 3781 4538 4897 5056 Size: 648 1420 32 1066 97 757 359 159 Cuts at: 5056 518 Size: 2042 Fragments arranged by size: 2042 1420 1066 757 648 359 159 97 32 MspA1I CmG'CkG Cuts at: 1606 3320 3996 4673 5821 6066 1606 Size: 1714 676 677 1148 245 2120 Fragments arranged by size: 2120 1714 1148 677 676 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5071 Fragments arranged by size: 5071 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6580 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6580 NotI GC'GGCC_GC Cuts at: 4027 4027 Size: 6580 NsiI A_TGCA'T Cuts at: 3095 3095 Size: 6580 NspI r_CATG'y Cuts at: 1132 1229 3510 3938 5483 1132 Size: 97 2281 428 1545 2229 Fragments arranged by size: 2281 2229 1545 428 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6580 PciI A'CATG_T Cuts at: 3506 5479 3506 Size: 1973 4607 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6580 PmeI GTTT'AAAC Cuts at: 3884 3884 Size: 6580 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4649 4681 6162 6194 4649 Size: 32 1481 32 5035 Fragments arranged by size: 5035 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3609 1613 Size: 1996 4584 PsiI TTA'TAA Cuts at: 3825 4150 3825 Size: 325 6255 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2834 2866 2834 Size: 32 6548 PstI C_TGCA'G Cuts at: 830 2299 3932 830 Size: 1469 1633 3478 Fragments arranged by size: 3478 1633 1469 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6580 PvuII CAG'CTG Cuts at: 3320 3320 Size: 6580 SalI G'TCGA_C Cuts at: 1936 3922 1936 Size: 1986 4594 SapI GCTCTTCn'nnn_ Cuts at: 2934 2934 Size: 6580 SbfI CC_TGCA'GG Cuts at: 3932 3932 Size: 6580 ScaI AGT'ACT Cuts at: 4828 6540 4828 Size: 1712 4868 SfcI C'TryA_G Cuts at: 826 1045 2295 3203 3928 5063 5744 5935 826 Size: 219 1250 908 725 1135 681 191 1471 Fragments arranged by size: 1471 1250 1135 908 725 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6580 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6580 SmaI CCC'GGG Cuts at: 1872 3907 1872 Size: 2035 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3440 4684 5585 5847 820 Size: 197 926 1497 1244 901 262 1553 Fragments arranged by size: 1553 1497 1244 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3877 4371 493 Size: 3384 494 2702 Fragments arranged by size: 3384 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6580 SphI G_CATG'C Cuts at: 1132 3938 1132 Size: 2806 3774 SspI AAT'ATT Cuts at: 5 52 2707 2869 4504 5 Size: 47 2655 162 1635 2081 Fragments arranged by size: 2655 2081 1635 162 47 StuI AGG'CCT Cuts at: 1517 3222 1517 Size: 1705 4875 StyI C'CwwG_G Cuts at: 513 1065 2022 2919 3452 4019 513 Size: 552 957 897 533 567 3074 Fragments arranged by size: 3074 957 897 567 552 533 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4607 4624 4777 4962 5407 325 Size: 1255 3027 17 153 185 445 1498 Fragments arranged by size: 3027 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3392 4826 6538 Size: 274 80 33 51 1443 1415 1434 1712 Cuts at: 6538 96 Size: 138 Fragments arranged by size: 1712 1443 1434 1415 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6540 6572 6540 Size: 32 6548 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6580 XbaI T'CTAG_A Cuts at: 2764 3397 3916 2764 Size: 633 519 5428 Fragments arranged by size: 5428 633 519 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6580 XmaI C'CCGG_G Cuts at: 1870 3905 1870 Size: 2035 4545 XmnI GAAnn'nnTTC Cuts at: 3006 3467 4709 3006 Size: 461 1242 4877 Fragments arranged by size: 4877 1242 461 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4388 276 Size: 53 83 186 936 2854 2468 Fragments arranged by size: 2854 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BbvCI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI