(Circular) (Six-base) MAPSORT of: pFN21ASDA1778.seq Check: 9970 from: 1 to: 5704 pFN21ASDA1778 5704 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2927 2927 Size: 5704 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2750 3514 278 Size: 53 83 186 936 1214 764 2468 Fragments arranged by size: 2468 1214 936 764 186 83 53 AccI GT'mk_AC Cuts at: 1937 3047 1937 Size: 1110 4594 Acc65I G'GTAC_C Cuts at: 3025 3025 Size: 5704 AclI AA'CG_TT Cuts at: 1563 3831 4204 1563 Size: 2268 373 3063 Fragments arranged by size: 3063 2268 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3767 5151 5419 1632 Size: 2135 1384 268 1917 Fragments arranged by size: 2135 1917 1384 268 AfeI AGC'GCT Cuts at: 2816 2816 Size: 5704 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5507 AflIII A'CryG_T Cuts at: 1203 2042 4603 5580 1203 Size: 839 2561 977 1327 Fragments arranged by size: 2561 1327 977 839 AgeI A'CCGG_T Cuts at: 4535 4535 Size: 5704 AhdI GACnn_n'nnGTC Cuts at: 2872 4433 2872 Size: 1561 4143 AleI CACnn'nnGTG Cuts at: 1166 2177 1166 Size: 1011 4693 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2375 1184 Size: 1191 4513 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2693 2725 2693 Size: 32 5672 AlwNI CAG_nnn'CTG Cuts at: 2236 2623 3447 5019 2236 Size: 387 824 1572 2921 Fragments arranged by size: 2921 1572 824 387 ApaI G_GGCC'C Cuts at: 2600 2600 Size: 5704 ApaLI G'TGCA_C Cuts at: 2459 3761 4917 2459 Size: 1302 1156 3246 Fragments arranged by size: 3246 1302 1156 ApoI r'AATT_y Cuts at: 1493 3013 3239 3391 5500 1493 Size: 1520 226 152 2109 1697 Fragments arranged by size: 2109 1697 1520 226 152 AseI AT'TA_AT Cuts at: 160 1032 4258 160 Size: 872 3226 1606 Fragments arranged by size: 3226 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 AvaI C'yCGr_G Cuts at: 1870 1943 2241 2345 2408 2431 2641 3029 1870 Size: 73 298 104 63 23 210 388 4545 Fragments arranged by size: 4545 388 298 210 104 73 63 23 BamHI G'GATC_C Cuts at: 3034 3401 3034 Size: 367 5337 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2185 2413 2528 2542 2915 Size: 325 217 627 398 228 115 14 373 Cuts at: 2915 3025 4480 618 Size: 110 1455 1842 Fragments arranged by size: 1842 1455 627 398 373 325 228 217 115 110 14 BanII G_rGCy'C Cuts at: 1391 2397 2474 2514 2600 3023 1391 Size: 1006 77 40 86 423 4072 Fragments arranged by size: 4072 1006 423 86 77 40 BbeI G_GCGC'C Cuts at: 2417 2532 2546 2417 Size: 115 14 5575 Fragments arranged by size: 5575 115 14 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2977 928 Size: 971 1078 3655 Fragments arranged by size: 3655 1078 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3895 3929 1903 Size: 34 1958 34 3678 Fragments arranged by size: 3678 1958 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3596 4806 3596 Size: 1210 4494 BclI T'GATC_A Cuts at: 1548 1548 Size: 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2449 4315 136 Size: 107 122 71 631 605 777 1866 1525 Fragments arranged by size: 1866 1525 777 631 605 122 107 71 BglII A'GATC_T Cuts at: 2958 5699 2958 Size: 2741 2963 BlpI GC'TnA_GC Cuts at: 3121 3121 Size: 5704 Bme1580I G_kGCm'C Cuts at: 1790 2190 2463 2600 2918 3765 4921 1790 Size: 400 273 137 318 847 1156 2573 Fragments arranged by size: 2573 1156 847 400 318 273 137 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2611 3435 4393 Size: 416 53 83 399 24 1194 824 958 Cuts at: 4393 442 Size: 1753 Fragments arranged by size: 1753 1194 958 824 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2571 1056 Size: 1515 4189 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2548 2548 Size: 5704 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2579 2873 4364 1239 Size: 1340 294 1491 2579 Fragments arranged by size: 2579 1491 1340 294 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2822 3829 4694 4992 2822 Size: 1007 865 298 3534 Fragments arranged by size: 3534 1007 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4367 882 Size: 617 2868 2219 Fragments arranged by size: 2868 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2045 2158 3001 3495 493 Size: 711 841 113 843 494 2702 Fragments arranged by size: 2702 843 841 711 494 113 BsaBI GATnn'nnATC Cuts at: 5698 5698 Size: 5704 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2414 2529 2543 2703 Size: 53 83 186 936 881 115 14 160 Cuts at: 2703 2747 3511 3893 4528 275 Size: 44 764 382 635 1451 Fragments arranged by size: 1451 936 881 764 635 382 186 160 115 83 53 44 14 BsaWI w'CCGG_w Cuts at: 4137 4535 4809 4956 5367 4137 Size: 398 274 147 411 4474 Fragments arranged by size: 4474 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2654 2684 1178 Size: 30 165 30 1251 30 4198 Fragments arranged by size: 4198 1251 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2077 1186 Size: 891 4813 BseYI C'CCAG_C Cuts at: 2418 2476 4907 5403 2418 Size: 58 2431 496 2719 Fragments arranged by size: 2719 2431 496 58 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2155 2923 1163 Size: 573 419 768 3944 Fragments arranged by size: 3944 768 573 419 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2803 3154 3915 4943 1540 Size: 275 186 802 351 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 351 275 186 BsiHKAI G_wGCw'C Cuts at: 2397 2463 3765 3850 4921 2397 Size: 66 1302 85 1071 3180 Fragments arranged by size: 3180 1302 1071 85 66 BsmI GAATG_Cn' Cuts at: 3215 3308 3215 Size: 93 5611 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2397 2463 2474 2514 2600 2918 Size: 399 400 207 66 11 40 86 318 Cuts at: 2918 3023 3765 3850 4921 1391 Size: 105 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 400 399 318 207 105 86 85 66 40 11 BspEI T'CCGG_A Cuts at: 5367 5367 Size: 5704 BspHI T'CATG_A Cuts at: 3593 5323 3593 Size: 1730 3974 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2316 2927 3059 844 Size: 1472 611 132 3489 Fragments arranged by size: 3489 1472 611 132 BsrBI CCG'CTC Cuts at: 1394 1823 2113 3591 1394 Size: 429 290 1478 3507 Fragments arranged by size: 3507 1478 429 290 BsrDI GCAATG_nn' Cuts at: 65 1248 4199 4373 65 Size: 1183 2951 174 1396 Fragments arranged by size: 2951 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2355 2634 4348 4535 1700 Size: 110 545 279 1714 187 2869 Fragments arranged by size: 2869 1714 545 279 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5704 BssHII G'CGCG_C Cuts at: 1925 2723 1925 Size: 798 4906 BssSI C'ACGA_G Cuts at: 2324 2396 3764 4776 2324 Size: 72 1368 1012 3252 Fragments arranged by size: 3252 1368 1012 72 BstAPI GCAn_nnn'nTGC Cuts at: 794 2326 794 Size: 1532 4172 BstBI TT'CG_AA Cuts at: 2007 4508 2007 Size: 2501 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5704 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2029 Size: 5704 BstYI r'GATC_y Cuts at: 1972 2958 3034 3401 3786 3803 5255 5341 5353 Size: 986 76 367 385 17 1452 86 12 Cuts at: 5353 5699 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 986 385 367 346 86 76 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2100 2665 2910 513 Size: 552 957 78 565 245 3307 Fragments arranged by size: 3307 957 565 552 245 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4965 Fragments arranged by size: 4965 683 56 BtsI GCAGTG_nn' Cuts at: 2086 3220 4014 4034 2086 Size: 1134 794 20 3756 Fragments arranged by size: 3756 1134 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5704 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5669 DraI TTT'AAA Cuts at: 3008 3008 Size: 5704 DraIII CAC_nnn'GTG Cuts at: 2896 2896 Size: 5704 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4711 809 Size: 507 3395 1802 Fragments arranged by size: 3395 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2754 3151 4040 Size: 54 1425 50 275 18 924 397 889 Cuts at: 4040 5669 8 Size: 1629 43 Fragments arranged by size: 1629 1425 924 889 397 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3151 1537 Size: 275 1339 4090 Fragments arranged by size: 4090 1339 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2120 2291 3634 1353 Size: 767 171 1343 3423 Fragments arranged by size: 3423 1343 767 171 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2048 2714 4291 4665 4811 387 Size: 1064 138 459 666 1577 374 146 1280 Fragments arranged by size: 1577 1280 1064 666 459 374 146 138 EcoICRI GAG'CTC Cuts at: 2395 2395 Size: 5704 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2579 2873 3767 4364 5151 5419 1239 Size: 393 947 294 894 597 787 268 1524 Fragments arranged by size: 1524 947 894 787 597 393 294 268 EcoNI CCTnn'n_nnAGG Cuts at: 2855 2855 Size: 5704 EcoO109I rG'GnC_Cy Cuts at: 2794 2794 Size: 5704 EcoRI G'AATT_C Cuts at: 3013 3391 3013 Size: 378 5326 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2863 2863 Size: 5704 FspI TGC'GCA Cuts at: 4210 4210 Size: 5704 HaeII r_GCGC'y Cuts at: 1678 2170 2417 2532 2546 2641 2818 4851 1678 Size: 492 247 115 14 95 177 2033 2531 Fragments arranged by size: 2531 2033 492 247 177 115 95 14 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2858 2890 4345 4377 4419 Size: 32 135 32 1286 32 1455 32 42 Cuts at: 4419 4451 1373 Size: 32 2626 Fragments arranged by size: 2626 1455 1286 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3048 3294 669 Size: 988 281 1110 246 3079 Fragments arranged by size: 3079 1110 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5704 HpaI GTT'AAC Cuts at: 3294 3294 Size: 5704 KasI G'GCGC_C Cuts at: 2413 2528 2542 2413 Size: 115 14 5575 Fragments arranged by size: 5575 115 14 KpnI G_GTAC'C Cuts at: 3029 3029 Size: 5704 MfeI C'AATT_G Cuts at: 3303 3303 Size: 5704 MluI A'CGCG_T Cuts at: 5580 5580 Size: 5704 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4818 5002 1305 Size: 3513 184 2007 Fragments arranged by size: 3513 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2756 10 Size: 54 1425 343 924 2958 Fragments arranged by size: 2958 1425 924 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2177 3662 4021 4180 518 Size: 648 1011 1485 359 159 2042 Fragments arranged by size: 2042 1485 1011 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2102 2602 2667 3120 3797 4945 5190 1606 Size: 496 500 65 453 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 500 496 453 245 65 NaeI GCC'GGC Cuts at: 2357 2636 2357 Size: 279 5425 NarI GG'CG_CC Cuts at: 2414 2529 2543 2414 Size: 115 14 5575 Fragments arranged by size: 5575 115 14 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4195 Fragments arranged by size: 4195 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5704 NgoMIV G'CCGG_C Cuts at: 2355 2634 2355 Size: 279 5425 NheI G'CTAG_C Cuts at: 1052 2567 1052 Size: 1515 4189 NotI GC'GGCC_GC Cuts at: 3151 3151 Size: 5704 NspI r_CATG'y Cuts at: 1132 1229 2212 3062 4607 1132 Size: 97 983 850 1545 2229 Fragments arranged by size: 2229 1545 983 850 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5704 PciI A'CATG_T Cuts at: 4603 4603 Size: 5704 PflMI CCAn_nnn'nTGG Cuts at: 1108 2623 2896 1108 Size: 1515 273 3916 Fragments arranged by size: 3916 1515 273 PfoI T'CCnGG_A Cuts at: 2298 2850 2298 Size: 552 5152 PmeI GTTT'AAAC Cuts at: 3008 3008 Size: 5704 PmlI CAC'GTG Cuts at: 2045 2045 Size: 5704 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2011 2043 3773 3805 5286 5318 2011 Size: 32 1730 32 1481 32 2397 Fragments arranged by size: 2397 1730 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2794 2794 Size: 5704 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5704 PsiI TTA'TAA Cuts at: 3274 3274 Size: 5704 PspOMI G'GGCC_C Cuts at: 2596 2596 Size: 5704 PstI C_TGCA'G Cuts at: 830 2364 2993 3056 830 Size: 1534 629 63 3478 Fragments arranged by size: 3478 1534 629 63 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5704 SacI G_AGCT'C Cuts at: 2397 2397 Size: 5704 SacII CC_GC'GG Cuts at: 2103 2668 2103 Size: 565 5139 SalI G'TCGA_C Cuts at: 1936 3046 1936 Size: 1110 4594 SanDI GG'GwC_CC Cuts at: 2794 2794 Size: 5704 SapI GCTCTTCn'nnn_ Cuts at: 2120 2120 Size: 5704 SbfI CC_TGCA'GG Cuts at: 3056 3056 Size: 5704 ScaI AGT'ACT Cuts at: 2378 3952 5664 2378 Size: 1574 1712 2418 Fragments arranged by size: 2418 1712 1574 SfcI C'TryA_G Cuts at: 826 1045 2360 2989 3052 4187 4868 5059 826 Size: 219 1315 629 63 1135 681 191 1471 Fragments arranged by size: 1471 1315 1135 681 629 219 191 63 SfoI GGC'GCC Cuts at: 2415 2530 2544 2415 Size: 115 14 5575 Fragments arranged by size: 5575 115 14 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5704 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5704 SmaI CCC'GGG Cuts at: 1872 2243 2433 3031 1872 Size: 371 190 598 4545 Fragments arranged by size: 4545 598 371 190 SmlI C'TyrA_G Cuts at: 820 1017 1943 2837 3808 4709 4971 820 Size: 197 926 894 971 901 262 1553 Fragments arranged by size: 1553 971 926 901 894 262 197 SnaBI TAC'GTA Cuts at: 493 3001 3495 493 Size: 2508 494 2702 Fragments arranged by size: 2702 2508 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5704 SphI G_CATG'C Cuts at: 1132 3062 1132 Size: 1930 3774 SspI AAT'ATT Cuts at: 5 52 3628 5 Size: 47 3576 2081 Fragments arranged by size: 3576 2081 47 StuI AGG'CCT Cuts at: 1517 2861 1517 Size: 1344 4360 StyI C'CwwG_G Cuts at: 513 1065 2022 2757 3143 513 Size: 552 957 735 386 3074 Fragments arranged by size: 3074 957 735 552 386 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2468 3731 3748 3901 4086 4531 325 Size: 1255 888 1263 17 153 185 445 1498 Fragments arranged by size: 1498 1263 1255 888 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2376 3950 5662 Size: 274 80 33 51 1443 399 1574 1712 Cuts at: 5662 96 Size: 138 Fragments arranged by size: 1712 1574 1443 399 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2100 2132 5664 5696 2100 Size: 32 3532 32 2108 Fragments arranged by size: 3532 2108 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5704 XbaI T'CTAG_A Cuts at: 3040 3040 Size: 5704 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2560 2560 Size: 5704 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5704 XmaI C'CCGG_G Cuts at: 1870 2241 2431 3029 1870 Size: 371 190 598 4545 Fragments arranged by size: 4545 598 371 190 XmnI GAAnn'nnTTC Cuts at: 3833 3833 Size: 5704 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2748 3512 276 Size: 53 83 186 936 1214 764 2468 Fragments arranged by size: 2468 1214 936 764 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI PvuII RsrII SexAI SfiI SrfI SwaI