(Circular) (Six-base) MAPSORT of: pFN21ASDA1759.seq Check: 740 from: 1 to: 7645 pFN21ASDA1759 7645 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3918 4405 3918 Size: 487 7158 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5455 278 Size: 53 83 186 936 3919 2468 Fragments arranged by size: 3919 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2077 4797 4988 1937 Size: 140 2720 191 4594 Fragments arranged by size: 4594 2720 191 140 Acc65I G'GTAC_C Cuts at: 4966 4966 Size: 7645 AclI AA'CG_TT Cuts at: 1563 5772 6145 1563 Size: 4209 373 3063 Fragments arranged by size: 4209 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3223 4652 4872 5708 7092 7360 1632 Size: 1591 1429 220 836 1384 268 1917 Fragments arranged by size: 1917 1591 1429 1384 836 268 220 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7448 AflIII A'CryG_T Cuts at: 1203 6544 7521 1203 Size: 5341 977 1327 Fragments arranged by size: 5341 1327 977 AgeI A'CCGG_T Cuts at: 6476 6476 Size: 7645 AhdI GACnn_n'nnGTC Cuts at: 6374 6374 Size: 7645 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7645 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3830 4214 1184 Size: 2646 384 4615 Fragments arranged by size: 4615 2646 384 AlwNI CAG_nnn'CTG Cuts at: 2201 2300 2455 3113 3553 4351 4420 4553 5388 Size: 99 155 658 440 798 69 133 835 Cuts at: 5388 6960 2201 Size: 1572 2886 Fragments arranged by size: 2886 1572 835 798 658 440 155 133 99 69 ApaI G_GGCC'C Cuts at: 2066 2307 3902 4541 4645 4756 4929 2066 Size: 241 1595 639 104 111 173 4782 Fragments arranged by size: 4782 1595 639 241 173 111 104 ApaLI G'TGCA_C Cuts at: 5702 6858 5702 Size: 1156 6489 ApoI r'AATT_y Cuts at: 1493 4820 4954 5180 5332 7441 1493 Size: 3327 134 226 152 2109 1697 Fragments arranged by size: 3327 2109 1697 226 152 134 AseI AT'TA_AT Cuts at: 160 1032 6199 160 Size: 872 5167 1606 Fragments arranged by size: 5167 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7645 AvaI C'yCGr_G Cuts at: 1870 1943 2058 2120 2190 2603 4268 4743 4970 Size: 73 115 62 70 413 1665 475 227 Cuts at: 4970 1870 Size: 4545 Fragments arranged by size: 4545 1665 475 413 227 115 73 70 62 BamHI G'GATC_C Cuts at: 4975 5342 4975 Size: 367 7278 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2070 2693 3016 3408 3976 Size: 325 217 627 283 623 323 392 568 Cuts at: 3976 4966 6421 618 Size: 990 1455 1842 Fragments arranged by size: 1842 1455 990 627 623 568 392 325 323 283 217 BanII G_rGCy'C Cuts at: 1391 2066 2307 2756 2817 3777 3902 4056 4173 Size: 675 241 449 61 960 125 154 117 Cuts at: 4173 4427 4541 4645 4712 4756 4929 4964 1391 Size: 254 114 104 67 44 173 35 4072 Fragments arranged by size: 4072 960 675 449 254 241 173 154 125 117 114 104 67 61 44 35 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2131 2642 2895 3672 4378 928 Size: 971 232 511 253 777 706 4195 Fragments arranged by size: 4195 971 777 706 511 253 232 BbvCI CC'TCA_GC Cuts at: 3257 4187 4533 3257 Size: 930 346 6369 Fragments arranged by size: 6369 930 346 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2171 2204 2205 2238 2265 2299 5836 Size: 34 234 33 1 33 27 34 3537 Cuts at: 5836 5870 1903 Size: 34 3678 Fragments arranged by size: 3678 3537 234 34 34 34 33 33 27 1 BciVI GTATCCnnnnn_n' Cuts at: 5537 6747 5537 Size: 1210 6435 BclI T'GATC_A Cuts at: 1548 1548 Size: 7645 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3449 3558 4331 Size: 107 122 71 631 605 1777 109 773 Cuts at: 4331 4649 6256 136 Size: 318 1607 1525 Fragments arranged by size: 1777 1607 1525 773 631 605 318 122 109 107 71 BglII A'GATC_T Cuts at: 7640 7640 Size: 7645 BlpI GC'TnA_GC Cuts at: 2042 2406 3323 5062 2042 Size: 364 917 1739 4625 Fragments arranged by size: 4625 1739 917 364 Bme1580I G_kGCm'C Cuts at: 1790 2066 2073 2307 2523 2696 2824 3454 3902 Size: 276 7 234 216 173 128 630 448 Cuts at: 3902 4541 4645 4756 4929 5706 6862 1790 Size: 639 104 111 173 777 1156 2573 Fragments arranged by size: 2573 1156 777 639 630 448 276 234 216 173 173 128 111 104 7 BmgBI CAC'GTC Cuts at: 1318 3698 1318 Size: 2380 5265 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2099 2517 3771 Size: 416 53 83 399 24 682 418 1254 Cuts at: 3771 4389 4537 5376 6334 442 Size: 618 148 839 958 1753 Fragments arranged by size: 1753 1254 958 839 682 618 418 416 399 148 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7645 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2460 3460 3781 4030 2460 Size: 1000 321 249 6075 Fragments arranged by size: 6075 1000 321 249 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2195 2333 2655 2954 3555 3601 3675 3698 Size: 956 138 322 299 601 46 74 23 Cuts at: 3698 4713 6305 1239 Size: 1015 1592 2579 Fragments arranged by size: 2579 1592 1015 956 601 322 299 138 74 46 23 Bpu10I CC'TnA_GC Cuts at: 2628 3257 3771 4167 4187 4533 2628 Size: 629 514 396 20 346 5740 Fragments arranged by size: 5740 629 514 396 346 20 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2067 4148 4811 5770 6635 6933 2067 Size: 2081 663 959 865 298 2779 Fragments arranged by size: 2779 2081 959 865 663 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2117 3229 6308 882 Size: 617 618 1112 3079 2219 Fragments arranged by size: 3079 2219 1112 618 617 BsaAI yAC'GTr Cuts at: 493 1204 4942 5436 493 Size: 711 3738 494 2702 Fragments arranged by size: 3738 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7639 7639 Size: 7645 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5452 5834 6469 275 Size: 53 83 186 936 3919 382 635 1451 Fragments arranged by size: 3919 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 6078 6476 6750 6897 7308 6078 Size: 398 274 147 411 6415 Fragments arranged by size: 6415 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7420 Fragments arranged by size: 7420 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3071 3472 3662 3836 3866 3883 4145 4148 Size: 1885 401 190 174 30 17 262 3 Cuts at: 4148 4151 1186 Size: 3 4680 Fragments arranged by size: 4680 1885 401 262 190 174 30 17 3 3 BseYI C'CCAG_C Cuts at: 2530 2747 2896 3419 3577 3862 3881 3921 3992 Size: 217 149 523 158 285 19 40 71 Cuts at: 3992 4326 4344 4420 4474 6848 7344 2530 Size: 334 18 76 54 2374 496 2831 Fragments arranged by size: 2831 2374 523 496 334 285 217 158 149 76 71 54 40 19 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2229 2295 2309 2996 3909 4824 1163 Size: 573 493 66 14 687 913 915 3984 Fragments arranged by size: 3984 915 913 687 573 493 66 14 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5095 5856 6884 1540 Size: 275 186 3094 761 1028 2301 Fragments arranged by size: 3094 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4056 4257 5706 5791 6862 4056 Size: 201 1449 85 1071 4839 Fragments arranged by size: 4839 1449 1071 201 85 BsmI GAATG_Cn' Cuts at: 4795 5156 5249 4795 Size: 361 93 7191 Fragments arranged by size: 7191 361 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4674 4674 Size: 7645 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2066 2073 2307 2523 2696 2756 2817 Size: 399 276 7 234 216 173 60 61 Cuts at: 2817 2824 3454 3777 3902 4056 4173 4257 4427 Size: 7 630 323 125 154 117 84 170 Cuts at: 4427 4541 4645 4712 4756 4929 4964 5706 5791 Size: 114 104 67 44 173 35 742 85 Cuts at: 5791 6862 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 630 399 323 276 234 216 173 173 170 154 125 117 114 104 85 84 67 61 60 44 35 7 7 BspEI T'CCGG_A Cuts at: 7308 7308 Size: 7645 BspHI T'CATG_A Cuts at: 2922 5534 7264 2922 Size: 2612 1730 3303 Fragments arranged by size: 3303 2612 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2135 3918 4405 4561 5000 844 Size: 1291 1783 487 156 439 3489 Fragments arranged by size: 3489 1783 1291 487 439 156 BsrBI CCG'CTC Cuts at: 1394 1823 2179 2642 3760 4359 5532 1394 Size: 429 356 463 1118 599 1173 3507 Fragments arranged by size: 3507 1173 1118 599 463 429 356 BsrDI GCAATG_nn' Cuts at: 65 1248 2372 2597 2950 4087 4506 6140 6314 Size: 1183 1124 225 353 1137 419 1634 174 Cuts at: 6314 65 Size: 1396 Fragments arranged by size: 1634 1396 1183 1137 1124 419 353 225 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3153 3334 6289 6476 1700 Size: 110 1343 181 2955 187 2869 Fragments arranged by size: 2955 2869 1343 187 181 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7645 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7645 BssSI C'ACGA_G Cuts at: 2327 3453 5705 6717 2327 Size: 1126 2252 1012 3255 Fragments arranged by size: 3255 2252 1126 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2878 4553 794 Size: 2084 1675 3886 Fragments arranged by size: 3886 2084 1675 BstBI TT'CG_AA Cuts at: 2007 6449 2007 Size: 4442 3203 BstEII G'GTnAC_C Cuts at: 1184 3905 1184 Size: 2721 4924 BstXI CCAn_nnnn'nTGG Cuts at: 3929 3945 3929 Size: 16 7629 BstYI r'GATC_y Cuts at: 1972 2332 4898 4975 5342 5727 5744 7196 7282 Size: 360 2566 77 367 385 17 1452 86 Cuts at: 7282 7294 7640 1972 Size: 12 346 1977 Fragments arranged by size: 2566 1977 1452 385 367 360 346 86 77 17 12 BstZ17I GTA'TAC Cuts at: 2078 2078 Size: 7645 Bsu36I CC'TnA_GG Cuts at: 3819 3951 3819 Size: 132 7513 BtgI C'CryG_G Cuts at: 513 1065 2022 4513 513 Size: 552 957 2491 3645 Fragments arranged by size: 3645 2491 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2758 487 Size: 683 56 1532 5374 Fragments arranged by size: 5374 1532 683 56 BtsI GCAGTG_nn' Cuts at: 2255 2834 4118 4478 5161 5955 5975 2255 Size: 579 1284 360 683 794 20 3925 Fragments arranged by size: 3925 1284 794 683 579 360 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7645 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7610 DraI TTT'AAA Cuts at: 4949 4949 Size: 7645 DraIII CAC_nnn'GTG Cuts at: 3701 3701 Size: 7645 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3716 6652 809 Size: 507 2400 2936 1802 Fragments arranged by size: 2936 2400 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2509 2805 3625 Size: 54 1425 50 275 18 679 296 820 Cuts at: 3625 5092 5981 7610 8 Size: 1467 889 1629 43 Fragments arranged by size: 1629 1467 1425 889 820 679 296 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5092 1537 Size: 275 3280 4090 Fragments arranged by size: 4090 3280 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2924 3242 3490 3539 3852 4577 4782 5575 Size: 1571 318 248 49 313 725 205 793 Cuts at: 5575 1353 Size: 3423 Fragments arranged by size: 3423 1571 793 725 318 313 248 205 49 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6232 6606 6752 387 Size: 1064 138 4643 374 146 1280 Fragments arranged by size: 4643 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4054 4054 Size: 7645 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2195 2333 2655 2954 3223 3555 3601 Size: 393 563 138 322 299 269 332 46 Cuts at: 3601 3675 3698 4652 4713 4872 5708 6305 7092 Size: 74 23 954 61 159 836 597 787 Cuts at: 7092 7360 1239 Size: 268 1524 Fragments arranged by size: 1524 954 836 787 597 563 393 332 322 299 269 268 159 138 74 61 46 23 EcoNI CCTnn'n_nnAGG Cuts at: 4130 4412 4545 4130 Size: 282 133 7230 Fragments arranged by size: 7230 282 133 EcoO109I rG'GnC_Cy Cuts at: 2062 2063 2186 2297 2304 3029 3243 3458 3898 Size: 1 123 111 7 725 214 215 440 Cuts at: 3898 3899 4537 4538 4550 4641 4752 4753 4926 Size: 1 638 1 12 91 111 1 173 Cuts at: 4926 2062 Size: 4781 Fragments arranged by size: 4781 725 638 440 215 214 173 123 111 111 91 12 7 1 1 1 1 EcoRI G'AATT_C Cuts at: 4954 5332 4954 Size: 378 7267 FspI TGC'GCA Cuts at: 2231 6151 2231 Size: 3920 3725 HaeII r_GCGC'y Cuts at: 1678 6792 1678 Size: 5114 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4883 4915 6286 6318 6360 Size: 32 135 32 3311 32 1371 32 42 Cuts at: 6360 6392 1373 Size: 32 2626 Fragments arranged by size: 3311 2626 1371 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2253 2352 4300 4989 5235 669 Size: 988 281 315 99 1948 689 246 3079 Fragments arranged by size: 3079 1948 988 689 315 281 246 99 HindIII A'AGCT_T Cuts at: 748 748 Size: 7645 HpaI GTT'AAC Cuts at: 5235 5235 Size: 7645 KpnI G_GTAC'C Cuts at: 4970 4970 Size: 7645 MfeI C'AATT_G Cuts at: 4658 5244 4658 Size: 586 7059 MluI A'CGCG_T Cuts at: 7521 7521 Size: 7645 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4503 4658 4860 6759 6943 1305 Size: 3198 155 202 1899 184 2007 Fragments arranged by size: 3198 2007 1899 202 184 155 MscI TGG'CCA Cuts at: 10 64 1489 1832 2807 10 Size: 54 1425 343 975 4848 Fragments arranged by size: 4848 1425 975 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3156 4250 4379 5603 5962 6121 518 Size: 648 1990 1094 129 1224 359 159 2042 Fragments arranged by size: 2042 1990 1224 1094 648 359 159 129 MspA1I CmG'CkG Cuts at: 1606 2362 2569 2747 3261 3283 3371 3714 4216 Size: 756 207 178 514 22 88 343 502 Cuts at: 4216 4330 5061 5738 6886 7131 1606 Size: 114 731 677 1148 245 2120 Fragments arranged by size: 2120 1148 756 731 677 514 502 343 245 207 178 114 88 22 NaeI GCC'GGC Cuts at: 3336 3336 Size: 7645 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 6136 Fragments arranged by size: 6136 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7645 NgoMIV G'CCGG_C Cuts at: 3334 3334 Size: 7645 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7645 NotI GC'GGCC_GC Cuts at: 5092 5092 Size: 7645 NspI r_CATG'y Cuts at: 1132 1229 3443 4279 4625 5003 6548 1132 Size: 97 2214 836 346 378 1545 2229 Fragments arranged by size: 2229 2214 1545 836 378 346 97 PasI CC'CwG_GG Cuts at: 1348 2066 2300 2698 4715 1348 Size: 718 234 398 2017 4278 Fragments arranged by size: 4278 2017 718 398 234 PciI A'CATG_T Cuts at: 6544 6544 Size: 7645 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7645 PfoI T'CCnGG_A Cuts at: 2172 3460 3791 4056 2172 Size: 1288 331 265 5761 Fragments arranged by size: 5761 1288 331 265 PmeI GTTT'AAAC Cuts at: 4949 4949 Size: 7645 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5714 5746 7227 7259 5714 Size: 32 1481 32 6100 Fragments arranged by size: 6100 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3029 3243 3458 4550 3029 Size: 214 215 1092 6124 Fragments arranged by size: 6124 1092 215 214 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7645 PsiI TTA'TAA Cuts at: 5215 5215 Size: 7645 PspOMI G'GGCC_C Cuts at: 2062 2303 3898 4537 4641 4752 4925 2062 Size: 241 1595 639 104 111 173 4782 Fragments arranged by size: 4782 1595 639 241 173 111 104 PspXI vC'TCGA_Gb Cuts at: 2603 2603 Size: 7645 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3078 3110 3078 Size: 32 7613 PstI C_TGCA'G Cuts at: 830 2619 3282 3646 4284 4416 4546 4997 830 Size: 1789 663 364 638 132 130 451 3478 Fragments arranged by size: 3478 1789 663 638 451 364 132 130 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7645 PvuII CAG'CTG Cuts at: 2362 2569 2747 3261 3283 3371 4216 4330 2362 Size: 207 178 514 22 88 845 114 5677 Fragments arranged by size: 5677 845 514 207 178 114 88 22 SacI G_AGCT'C Cuts at: 4056 4056 Size: 7645 SalI G'TCGA_C Cuts at: 1936 4987 1936 Size: 3051 4594 SapI GCTCTTCn'nnn_ Cuts at: 3852 4577 4782 3852 Size: 725 205 6715 Fragments arranged by size: 6715 725 205 SbfI CC_TGCA'GG Cuts at: 4997 4997 Size: 7645 ScaI AGT'ACT Cuts at: 5893 7605 5893 Size: 1712 5933 SexAI A'CCwGG_T Cuts at: 3734 3734 Size: 7645 SfcI C'TryA_G Cuts at: 826 1045 2615 3278 3642 4280 4412 4542 4993 Size: 219 1570 663 364 638 132 130 451 Cuts at: 4993 6128 6809 7000 826 Size: 1135 681 191 1471 Fragments arranged by size: 1570 1471 1135 681 663 638 451 364 219 191 132 130 SfiI GGCCn_nnn'nGGCC Cuts at: 4331 4331 Size: 7645 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7645 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7645 SmaI CCC'GGG Cuts at: 1872 2060 4745 4972 1872 Size: 188 2685 227 4545 Fragments arranged by size: 4545 2685 227 188 SmlI C'TyrA_G Cuts at: 820 1017 1943 2082 2603 4127 4268 4826 5749 Size: 197 926 139 521 1524 141 558 923 Cuts at: 5749 6650 6912 820 Size: 901 262 1553 Fragments arranged by size: 1553 1524 926 923 901 558 521 262 197 141 139 SnaBI TAC'GTA Cuts at: 493 4942 5436 493 Size: 4449 494 2702 Fragments arranged by size: 4449 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7645 SphI G_CATG'C Cuts at: 1132 3443 4625 5003 1132 Size: 2311 1182 378 3774 Fragments arranged by size: 3774 2311 1182 378 SspI AAT'ATT Cuts at: 5 52 5569 5 Size: 47 5517 2081 Fragments arranged by size: 5517 2081 47 StuI AGG'CCT Cuts at: 1517 4142 1517 Size: 2625 5020 StyI C'CwwG_G Cuts at: 513 1065 2022 4599 4765 5084 513 Size: 552 957 2577 166 319 3074 Fragments arranged by size: 3074 2577 957 552 319 166 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3179 3545 5672 5689 5842 6027 6472 Size: 1255 1599 366 2127 17 153 185 445 Cuts at: 6472 325 Size: 1498 Fragments arranged by size: 2127 1599 1498 1255 445 366 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2826 5891 7603 Size: 274 80 33 51 1443 849 3065 1712 Cuts at: 7603 96 Size: 138 Fragments arranged by size: 3065 1712 1443 849 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4647 4679 7605 7637 4647 Size: 32 2926 32 4655 Fragments arranged by size: 4655 2926 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7645 XbaI T'CTAG_A Cuts at: 4981 4981 Size: 7645 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2865 4232 2865 Size: 1367 6278 XhoI C'TCGA_G Cuts at: 1943 2603 4268 1943 Size: 660 1665 5320 Fragments arranged by size: 5320 1665 660 XmaI C'CCGG_G Cuts at: 1870 2058 4743 4970 1870 Size: 188 2685 227 4545 Fragments arranged by size: 4545 2685 227 188 XmnI GAAnn'nnTTC Cuts at: 5774 5774 Size: 7645 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5453 276 Size: 53 83 186 936 3919 2468 Fragments arranged by size: 3919 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI RsrII SacII SanDI SfoI SrfI SwaI