(Circular) (Six-base) MAPSORT of: pFN21ASDA1748.seq Check: 9531 from: 1 to: 6874 pFN21ASDA1748 6874 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2164 2354 2164 Size: 190 6684 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4684 278 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4217 1937 Size: 2280 4594 Acc65I G'GTAC_C Cuts at: 4145 4195 4145 Size: 50 6824 AclI AA'CG_TT Cuts at: 1563 5001 5374 1563 Size: 3438 373 3063 Fragments arranged by size: 3438 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2191 3435 4040 4937 6321 6589 1632 Size: 559 1244 605 897 1384 268 1917 Fragments arranged by size: 1917 1384 1244 897 605 559 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6677 AflIII A'CryG_T Cuts at: 1203 2126 5773 6750 1203 Size: 923 3647 977 1327 Fragments arranged by size: 3647 1327 977 923 AgeI A'CCGG_T Cuts at: 5705 5705 Size: 6874 AhdI GACnn_n'nnGTC Cuts at: 3628 5603 3628 Size: 1975 4899 AleI CACnn'nnGTG Cuts at: 1166 2382 1166 Size: 1216 5658 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2339 1184 Size: 1155 5719 AlwNI CAG_nnn'CTG Cuts at: 2036 2388 2951 3488 4617 6189 2036 Size: 352 563 537 1129 1572 2721 Fragments arranged by size: 2721 1572 1129 563 537 352 ApaLI G'TGCA_C Cuts at: 4931 6087 4931 Size: 1156 5718 ApoI r'AATT_y Cuts at: 1493 2408 2693 4183 4409 4561 6670 1493 Size: 915 285 1490 226 152 2109 1697 Fragments arranged by size: 2109 1697 1490 915 285 226 152 AseI AT'TA_AT Cuts at: 160 1032 5428 160 Size: 872 4396 1606 Fragments arranged by size: 4396 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 AvaI C'yCGr_G Cuts at: 1870 1943 2244 3694 3751 4199 1870 Size: 73 301 1450 57 448 4545 Fragments arranged by size: 4545 1450 448 301 73 57 BamHI G'GATC_C Cuts at: 4204 4571 4204 Size: 367 6507 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3371 3564 3577 4145 4195 Size: 325 217 627 1584 193 13 568 50 Cuts at: 4195 5650 618 Size: 1455 1842 Fragments arranged by size: 1842 1584 1455 627 568 325 217 193 50 13 BanII G_rGCy'C Cuts at: 1391 2287 3222 3606 4193 1391 Size: 896 935 384 587 4072 Fragments arranged by size: 4072 935 896 587 384 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2807 4028 928 Size: 971 908 1221 3774 Fragments arranged by size: 3774 1221 971 908 BbvCI CC'TCA_GC Cuts at: 2250 2894 2250 Size: 644 6230 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3341 3375 5065 5099 1903 Size: 34 1404 34 1690 34 3678 Fragments arranged by size: 3678 1690 1404 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4766 5976 4766 Size: 1210 5664 BclI T'GATC_A Cuts at: 1548 2413 1548 Size: 865 6009 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3539 3859 5485 Size: 107 122 71 631 605 1867 320 1626 Cuts at: 5485 136 Size: 1525 Fragments arranged by size: 1867 1626 1525 631 605 320 122 107 71 BglII A'GATC_T Cuts at: 6869 6869 Size: 6874 BlpI GC'TnA_GC Cuts at: 4066 4291 4066 Size: 225 6649 Bme1580I G_kGCm'C Cuts at: 1790 2166 3329 3374 4935 6091 1790 Size: 376 1163 45 1561 1156 2573 Fragments arranged by size: 2573 1561 1163 1156 376 45 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6874 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3830 4605 5563 Size: 416 53 83 399 24 2413 775 958 Cuts at: 5563 442 Size: 1753 Fragments arranged by size: 2413 1753 958 775 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6874 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2572 3193 2572 Size: 621 6253 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2748 2942 3118 3289 3638 5534 1239 Size: 1509 194 176 171 349 1896 2579 Fragments arranged by size: 2579 1896 1509 349 194 176 171 Bpu10I CC'TnA_GC Cuts at: 2250 2894 2970 2981 3489 3863 4060 2250 Size: 644 76 11 508 374 197 5064 Fragments arranged by size: 5064 644 508 374 197 76 11 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3296 4999 5864 6162 3296 Size: 1703 865 298 4008 Fragments arranged by size: 4008 1703 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2077 2779 5537 882 Size: 617 578 702 2758 2219 Fragments arranged by size: 2758 2219 702 617 578 BsaAI yAC'GTr Cuts at: 493 1204 2127 2644 4171 4665 493 Size: 711 923 517 1527 494 2702 Fragments arranged by size: 2702 1527 923 711 517 494 BsaBI GATnn'nnATC Cuts at: 6868 6868 Size: 6874 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3826 4681 5063 5698 Size: 53 83 186 936 2293 855 382 635 Cuts at: 5698 275 Size: 1451 Fragments arranged by size: 2293 1451 936 855 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5307 5705 5979 6126 6537 5307 Size: 398 274 147 411 5644 Fragments arranged by size: 5644 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3675 3705 1178 Size: 30 165 30 2272 30 4347 Fragments arranged by size: 4347 2272 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 2911 2955 3292 3769 4131 1186 Size: 1081 644 44 337 477 362 3929 Fragments arranged by size: 3929 1081 644 477 362 337 44 BseYI C'CCAG_C Cuts at: 2054 3203 3221 3340 3543 3829 6077 6573 2054 Size: 1149 18 119 203 286 2248 496 2355 Fragments arranged by size: 2355 2248 1149 496 286 203 119 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3497 1163 Size: 573 1761 4540 Fragments arranged by size: 4540 1761 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3089 3923 4324 5085 6113 1540 Size: 275 186 1088 834 401 761 1028 2301 Fragments arranged by size: 2301 1088 1028 834 761 401 275 186 BsiHKAI G_wGCw'C Cuts at: 2287 4935 5020 6091 2287 Size: 2648 85 1071 3070 Fragments arranged by size: 3070 2648 1071 85 BsmI GAATG_Cn' Cuts at: 3127 3732 4385 4478 3127 Size: 605 653 93 5523 Fragments arranged by size: 5523 653 605 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2166 2287 3222 3329 3374 3606 4193 Size: 399 376 121 935 107 45 232 587 Cuts at: 4193 4935 5020 6091 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 935 742 587 399 376 232 121 107 85 45 BspEI T'CCGG_A Cuts at: 6537 6537 Size: 6874 BspHI T'CATG_A Cuts at: 4763 6493 4763 Size: 1730 5144 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2164 2354 4229 844 Size: 1320 190 1875 3489 Fragments arranged by size: 3489 1875 1320 190 BsrBI CCG'CTC Cuts at: 1394 1823 3213 4761 1394 Size: 429 1390 1548 3507 Fragments arranged by size: 3507 1548 1390 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3421 5369 5543 65 Size: 1183 2173 1948 174 1396 Fragments arranged by size: 2173 1948 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5518 5705 1700 Size: 110 3708 187 2869 Fragments arranged by size: 3708 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3075 96 Size: 2979 3895 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6874 BssSI C'ACGA_G Cuts at: 4108 4934 5946 4108 Size: 826 1012 5036 Fragments arranged by size: 5036 1012 826 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6874 BstBI TT'CG_AA Cuts at: 2007 5678 2007 Size: 3671 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6874 BstXI CCAn_nnnn'nTGG Cuts at: 2224 2779 2224 Size: 555 6319 BstYI r'GATC_y Cuts at: 1972 4204 4571 4956 4973 6425 6511 6523 6869 Size: 2232 367 385 17 1452 86 12 346 Cuts at: 6869 1972 Size: 1977 Fragments arranged by size: 2232 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6322 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6135 Fragments arranged by size: 6135 683 56 BtsI GCAGTG_nn' Cuts at: 2166 2377 2389 2419 4390 5184 5204 2166 Size: 211 12 30 1971 794 20 3836 Fragments arranged by size: 3836 1971 794 211 30 20 12 ClaI AT'CG_AT Cuts at: 1554 3923 1554 Size: 2369 4505 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6839 DraI TTT'AAA Cuts at: 4178 4178 Size: 6874 DraIII CAC_nnn'GTG Cuts at: 3643 3643 Size: 6874 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5881 809 Size: 507 4565 1802 Fragments arranged by size: 4565 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2214 2655 Size: 54 1425 50 275 18 369 15 441 Cuts at: 2655 3045 3086 3399 4321 5210 6839 8 Size: 390 41 313 922 889 1629 43 Fragments arranged by size: 1629 1425 922 889 441 390 369 313 275 54 50 43 41 18 15 EagI C'GGCC_G Cuts at: 1537 1812 3086 4321 1537 Size: 275 1274 1235 4090 Fragments arranged by size: 4090 1274 1235 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2117 4804 1353 Size: 764 2687 3423 Fragments arranged by size: 3423 2687 764 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5461 5835 5981 387 Size: 1064 138 3872 374 146 1280 Fragments arranged by size: 3872 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2285 2285 Size: 6874 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2191 2748 2942 3118 3289 3435 3638 Size: 393 559 557 194 176 171 146 203 Cuts at: 3638 4040 4937 5534 6321 6589 1239 Size: 402 897 597 787 268 1524 Fragments arranged by size: 1524 897 787 597 559 557 402 393 268 203 194 176 171 146 EcoNI CCTnn'n_nnAGG Cuts at: 3891 4080 3891 Size: 189 6685 EcoO109I rG'GnC_Cy Cuts at: 3485 3882 3485 Size: 397 6477 EcoRI G'AATT_C Cuts at: 4183 4561 4183 Size: 378 6496 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3444 3444 Size: 6874 FspI TGC'GCA Cuts at: 2327 5380 2327 Size: 3053 3821 FspAI rTGC'GCAy Cuts at: 2327 2327 Size: 6874 HaeII r_GCGC'y Cuts at: 1678 3141 6021 1678 Size: 1463 2880 2531 Fragments arranged by size: 2880 2531 1463 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2227 2259 2924 2956 3181 Size: 32 135 32 655 32 665 32 225 Cuts at: 3181 3213 3269 3301 3455 3487 3614 3646 5515 Size: 32 56 32 154 32 127 32 1869 Cuts at: 5515 5547 5589 5621 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1869 665 655 225 154 135 127 56 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3741 4218 4464 669 Size: 988 281 1803 477 246 3079 Fragments arranged by size: 3079 1803 988 477 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6874 HpaI GTT'AAC Cuts at: 4464 4464 Size: 6874 KpnI G_GTAC'C Cuts at: 4149 4199 4149 Size: 50 6824 MfeI C'AATT_G Cuts at: 2805 3180 4473 2805 Size: 375 1293 5206 Fragments arranged by size: 5206 1293 375 MluI A'CGCG_T Cuts at: 6750 6750 Size: 6874 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4065 5988 6172 1305 Size: 2760 1923 184 2007 Fragments arranged by size: 2760 2007 1923 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2216 2657 3047 3401 Size: 54 1425 343 369 15 441 390 354 Cuts at: 3401 10 Size: 3483 Fragments arranged by size: 3483 1425 441 390 369 354 343 54 15 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2321 2382 4832 5191 5350 518 Size: 648 1056 99 61 2450 359 159 2042 Fragments arranged by size: 2450 2042 1056 648 359 159 99 61 MspA1I CmG'CkG Cuts at: 1606 3116 3833 4018 4290 4967 6115 6360 1606 Size: 1510 717 185 272 677 1148 245 2120 Fragments arranged by size: 2120 1510 1148 717 677 272 245 185 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6322 NdeI CA'TA_TG Cuts at: 387 387 Size: 6874 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6874 NotI GC'GGCC_GC Cuts at: 4321 4321 Size: 6874 NspI r_CATG'y Cuts at: 1132 1229 4232 5777 1132 Size: 97 3003 1545 2229 Fragments arranged by size: 3003 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2231 2427 3322 1348 Size: 883 196 895 4900 Fragments arranged by size: 4900 895 883 196 PciI A'CATG_T Cuts at: 5773 5773 Size: 6874 PflMI CCAn_nnn'nTGG Cuts at: 1108 2503 3643 1108 Size: 1395 1140 4339 Fragments arranged by size: 4339 1395 1140 PfoI T'CCnGG_A Cuts at: 2131 3654 2131 Size: 1523 5351 PmeI GTTT'AAAC Cuts at: 4178 4178 Size: 6874 PmlI CAC'GTG Cuts at: 2644 2644 Size: 6874 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3229 3261 4943 4975 6456 6488 3229 Size: 32 1682 32 1481 32 3615 Fragments arranged by size: 3615 1682 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3882 3882 Size: 6874 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6874 PsiI TTA'TAA Cuts at: 4444 4444 Size: 6874 PspXI vC'TCGA_Gb Cuts at: 2244 2244 Size: 6874 PstI C_TGCA'G Cuts at: 830 2080 2161 2384 2759 2947 4226 830 Size: 1250 81 223 375 188 1279 3478 Fragments arranged by size: 3478 1279 1250 375 223 188 81 PvuI CG_AT'CG Cuts at: 2001 3923 2001 Size: 1922 4952 PvuII CAG'CTG Cuts at: 3116 3833 4018 3116 Size: 717 185 5972 Fragments arranged by size: 5972 717 185 SacI G_AGCT'C Cuts at: 2287 2287 Size: 6874 SalI G'TCGA_C Cuts at: 1936 4216 1936 Size: 2280 4594 SanDI GG'GwC_CC Cuts at: 3882 3882 Size: 6874 SbfI CC_TGCA'GG Cuts at: 4226 4226 Size: 6874 ScaI AGT'ACT Cuts at: 5122 6834 5122 Size: 1712 5162 SexAI A'CCwGG_T Cuts at: 3952 3952 Size: 6874 SfcI C'TryA_G Cuts at: 826 1045 2076 2157 2380 2619 2755 2943 4030 Size: 219 1031 81 223 239 136 188 1087 Cuts at: 4030 4222 5357 6038 6229 826 Size: 192 1135 681 191 1471 Fragments arranged by size: 1471 1135 1087 1031 681 239 223 219 192 191 188 136 81 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6874 SmaI CCC'GGG Cuts at: 1872 3696 4201 1872 Size: 1824 505 4545 Fragments arranged by size: 4545 1824 505 SmlI C'TyrA_G Cuts at: 820 1017 1943 2244 3311 4978 5879 6141 820 Size: 197 926 301 1067 1667 901 262 1553 Fragments arranged by size: 1667 1553 1067 926 901 301 262 197 SnaBI TAC'GTA Cuts at: 493 4171 4665 493 Size: 3678 494 2702 Fragments arranged by size: 3678 2702 494 SpeI A'CTAG_T Cuts at: 152 3770 152 Size: 3618 3256 SphI G_CATG'C Cuts at: 1132 4232 1132 Size: 3100 3774 SspI AAT'ATT Cuts at: 5 52 4798 5 Size: 47 4746 2081 Fragments arranged by size: 4746 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6874 StyI C'CwwG_G Cuts at: 513 1065 3048 4313 513 Size: 552 1983 1265 3074 Fragments arranged by size: 3074 1983 1265 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2419 2495 2785 3582 4015 4901 4918 Size: 1255 839 76 290 797 433 886 17 Cuts at: 4918 5071 5256 5701 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 886 839 797 445 433 290 185 153 76 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3075 4012 5120 Size: 274 80 33 51 1443 1098 937 1108 Cuts at: 5120 6832 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1108 1098 937 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2628 2660 3675 3707 6834 6866 2628 Size: 32 1015 32 3127 32 2636 Fragments arranged by size: 3127 2636 1015 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6874 XbaI T'CTAG_A Cuts at: 4210 4210 Size: 6874 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2092 2209 3413 2092 Size: 117 1204 5553 Fragments arranged by size: 5553 1204 117 XhoI C'TCGA_G Cuts at: 1943 2244 1943 Size: 301 6573 XmaI C'CCGG_G Cuts at: 1870 3694 4199 1870 Size: 1824 505 4545 Fragments arranged by size: 4545 1824 505 XmnI GAAnn'nnTTC Cuts at: 2714 5003 2714 Size: 2289 4585 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4682 276 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoRV FseI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PsrI RsrII SacII SapI SfiI SfoI SrfI SwaI