(Circular) (Six-base) MAPSORT of: pFN21ASDA1733.seq Check: 4726 from: 1 to: 6181 pFN21ASDA1733 6181 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3991 278 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3197 3524 1937 Size: 1260 327 4594 Fragments arranged by size: 4594 1260 327 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 4308 4681 1563 Size: 2745 373 3063 Fragments arranged by size: 3063 2745 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2519 4244 5628 5896 1632 Size: 887 1725 1384 268 1917 Fragments arranged by size: 1917 1725 1384 887 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 2427 5080 6057 1203 Size: 1224 2653 977 1327 Fragments arranged by size: 2653 1327 1224 977 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 4910 4910 Size: 6181 AleI CACnn'nnGTG Cuts at: 1166 3063 1166 Size: 1897 4284 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6181 AlwNI CAG_nnn'CTG Cuts at: 2572 2690 3001 3924 5496 2572 Size: 118 311 923 1572 3257 Fragments arranged by size: 3257 1572 923 311 118 ApaLI G'TGCA_C Cuts at: 4238 5394 4238 Size: 1156 5025 ApoI r'AATT_y Cuts at: 1493 3490 3716 3868 5977 1493 Size: 1997 226 152 2109 1697 Fragments arranged by size: 2109 1997 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2159 4735 160 Size: 872 1127 2576 1606 Fragments arranged by size: 2576 1606 1127 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 2944 3061 3506 1870 Size: 73 1001 117 445 4545 Fragments arranged by size: 4545 1001 445 117 73 BamHI G'GATC_C Cuts at: 2635 3511 3878 2635 Size: 876 367 4938 Fragments arranged by size: 4938 876 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2324 2693 3398 3502 4957 Size: 325 217 627 537 369 705 104 1455 Cuts at: 4957 618 Size: 1842 Fragments arranged by size: 1842 1455 705 627 537 369 325 217 104 BanII G_rGCy'C Cuts at: 1391 2122 2579 2970 3500 1391 Size: 731 457 391 530 4072 Fragments arranged by size: 4072 731 530 457 391 BbeI G_GCGC'C Cuts at: 2697 2697 Size: 6181 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3186 3237 928 Size: 971 1287 51 3872 Fragments arranged by size: 3872 1287 971 51 BbvCI CC'TCA_GC Cuts at: 2274 3383 2274 Size: 1109 5072 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4372 4406 1903 Size: 34 2435 34 3678 Fragments arranged by size: 3678 2435 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4073 5283 4073 Size: 1210 4971 BclI T'GATC_A Cuts at: 1548 1548 Size: 6181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4792 136 Size: 107 122 71 631 605 3120 1525 Fragments arranged by size: 3120 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6176 6176 Size: 6181 BlpI GC'TnA_GC Cuts at: 3419 3598 3419 Size: 179 6002 Bme1580I G_kGCm'C Cuts at: 1790 4242 5398 1790 Size: 2452 1156 2573 Fragments arranged by size: 2573 2452 1156 BmgBI CAC'GTC Cuts at: 1318 2430 1318 Size: 1112 5069 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3912 4870 442 Size: 416 53 83 399 24 2495 958 1753 Fragments arranged by size: 2495 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2563 3398 4841 1239 Size: 1324 835 1443 2579 Fragments arranged by size: 2579 1443 1324 835 Bpu10I CC'TnA_GC Cuts at: 2274 2573 3292 3383 2274 Size: 299 719 91 5072 Fragments arranged by size: 5072 719 299 91 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2106 4306 5171 5469 2106 Size: 2200 865 298 2818 Fragments arranged by size: 2818 2200 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4844 882 Size: 617 3345 2219 Fragments arranged by size: 3345 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3478 3972 493 Size: 711 2274 494 2702 Fragments arranged by size: 2702 2274 711 494 BsaBI GATnn'nnATC Cuts at: 6175 6175 Size: 6181 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2694 3988 4370 5005 Size: 53 83 186 936 1161 1294 382 635 Cuts at: 5005 275 Size: 1451 Fragments arranged by size: 1451 1294 1161 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2202 4614 5012 5286 5433 5844 2202 Size: 2412 398 274 147 411 2539 Fragments arranged by size: 2539 2412 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2897 2927 3242 3272 3302 Size: 30 165 30 1494 30 315 30 30 Cuts at: 3302 3332 1178 Size: 30 4027 Fragments arranged by size: 4027 1494 315 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2505 3224 3233 3236 3383 1186 Size: 1319 719 9 3 147 3984 Fragments arranged by size: 3984 1319 719 147 9 3 BseYI C'CCAG_C Cuts at: 2838 5384 5880 2838 Size: 2546 496 3139 Fragments arranged by size: 3139 2546 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5608 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3631 4392 5420 1540 Size: 275 186 1630 761 1028 2301 Fragments arranged by size: 2301 1630 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2122 2579 2658 2970 4242 4327 5398 2122 Size: 457 79 312 1272 85 1071 2905 Fragments arranged by size: 2905 1272 1071 457 312 85 79 BsmI GAATG_Cn' Cuts at: 3692 3785 3692 Size: 93 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2122 2579 2658 2970 3500 4242 4327 Size: 399 332 457 79 312 530 742 85 Cuts at: 4327 5398 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 530 457 399 332 312 85 79 BspEI T'CCGG_A Cuts at: 2202 5844 2202 Size: 3642 2539 BspHI T'CATG_A Cuts at: 2757 3163 4070 5800 2757 Size: 406 907 1730 3138 Fragments arranged by size: 3138 1730 907 406 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3536 844 Size: 2692 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3373 4068 1394 Size: 429 1550 695 3507 Fragments arranged by size: 3507 1550 695 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4676 4850 65 Size: 1183 3428 174 1396 Fragments arranged by size: 3428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2190 2921 4825 5012 1700 Size: 110 380 731 1904 187 2869 Fragments arranged by size: 2869 1904 731 380 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6181 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6181 BssSI C'ACGA_G Cuts at: 4241 5253 4241 Size: 1012 5169 BstAPI GCAn_nnn'nTGC Cuts at: 794 2881 794 Size: 2087 4094 BstBI TT'CG_AA Cuts at: 2007 4985 2007 Size: 2978 3203 BstEII G'GTnAC_C Cuts at: 1184 3073 1184 Size: 1889 4292 BstXI CCAn_nnnn'nTGG Cuts at: 2161 2819 3050 3262 2161 Size: 658 231 212 5080 Fragments arranged by size: 5080 658 231 212 BstYI r'GATC_y Cuts at: 1972 2250 2635 2686 2868 3511 3878 4263 4280 Size: 278 385 51 182 643 367 385 17 Cuts at: 4280 5732 5818 5830 6176 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 643 385 385 367 346 278 182 86 51 17 12 BstZ17I GTA'TAC Cuts at: 3198 3198 Size: 6181 BtgI C'CryG_G Cuts at: 513 1065 2022 2772 2896 513 Size: 552 957 750 124 3798 Fragments arranged by size: 3798 957 750 552 124 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2312 487 Size: 683 56 1086 4356 Fragments arranged by size: 4356 1086 683 56 BtsI GCAGTG_nn' Cuts at: 3697 4491 4511 3697 Size: 794 20 5367 Fragments arranged by size: 5367 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6146 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2315 5188 809 Size: 507 999 2873 1802 Fragments arranged by size: 2873 1802 999 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2821 2899 2982 Size: 54 1425 50 275 18 991 78 83 Cuts at: 2982 3264 3628 4517 6146 8 Size: 282 364 889 1629 43 Fragments arranged by size: 1629 1425 991 889 364 282 275 83 78 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3628 1537 Size: 275 1816 4090 Fragments arranged by size: 4090 1816 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2469 2538 2810 3190 3201 3210 4111 1353 Size: 1116 69 272 380 11 9 901 3423 Fragments arranged by size: 3423 1116 901 380 272 69 11 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4768 5142 5288 387 Size: 1064 138 3179 374 146 1280 Fragments arranged by size: 3179 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2120 2577 2968 2120 Size: 457 391 5333 Fragments arranged by size: 5333 457 391 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 2563 3398 4244 4841 5628 5896 Size: 393 887 44 835 846 597 787 268 Cuts at: 5896 1239 Size: 1524 Fragments arranged by size: 1524 887 846 835 787 597 393 268 44 EcoNI CCTnn'n_nnAGG Cuts at: 2240 2240 Size: 6181 EcoO109I rG'GnC_Cy Cuts at: 3022 3086 3315 3022 Size: 64 229 5888 Fragments arranged by size: 5888 229 64 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3307 3307 Size: 6181 FspI TGC'GCA Cuts at: 4687 4687 Size: 6181 HaeII r_GCGC'y Cuts at: 1678 2697 5328 1678 Size: 1019 2631 2531 Fragments arranged by size: 2631 2531 1019 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2072 2077 2104 2627 Size: 32 135 32 473 27 5 27 523 Cuts at: 2627 2659 3227 3259 4822 4854 4896 4928 1373 Size: 32 568 32 1563 32 42 32 2626 Fragments arranged by size: 2626 1563 568 523 473 135 42 32 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 3525 3771 669 Size: 988 281 1587 246 3079 Fragments arranged by size: 3079 1587 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6181 HpaI GTT'AAC Cuts at: 3771 3771 Size: 6181 KasI G'GCGC_C Cuts at: 2693 2693 Size: 6181 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 6057 6057 Size: 6181 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2683 5295 5479 1305 Size: 1378 2612 184 2007 Fragments arranged by size: 2612 2007 1378 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2823 2984 3266 10 Size: 54 1425 343 991 161 282 2925 Fragments arranged by size: 2925 1425 991 343 282 161 54 MslI CAynn'nnrTG Cuts at: 518 1166 2159 2990 3063 4139 4498 4657 518 Size: 648 993 831 73 1076 359 159 2042 Fragments arranged by size: 2042 1076 993 831 648 359 159 73 MspA1I CmG'CkG Cuts at: 1606 2273 2587 2881 3019 3597 4274 5422 5667 Size: 667 314 294 138 578 677 1148 245 Cuts at: 5667 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 667 578 314 294 245 138 NarI GG'CG_CC Cuts at: 2694 2694 Size: 6181 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4672 Fragments arranged by size: 4672 957 552 NdeI CA'TA_TG Cuts at: 387 2647 387 Size: 2260 3921 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6181 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 3539 5084 1132 Size: 97 2310 1545 2229 Fragments arranged by size: 2310 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2631 1348 Size: 1283 4898 PciI A'CATG_T Cuts at: 5080 5080 Size: 6181 PflMI CCAn_nnn'nTGG Cuts at: 1108 2690 1108 Size: 1582 4599 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4250 4282 5763 5795 4250 Size: 32 1481 32 4636 Fragments arranged by size: 4636 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3022 3086 3022 Size: 64 6117 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PstI C_TGCA'G Cuts at: 830 2886 2954 3281 3439 3533 830 Size: 2056 68 327 158 94 3478 Fragments arranged by size: 3478 2056 327 158 94 68 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 PvuII CAG'CTG Cuts at: 2273 2587 2881 2273 Size: 314 294 5573 Fragments arranged by size: 5573 314 294 SacI G_AGCT'C Cuts at: 2122 2579 2970 2122 Size: 457 391 5333 Fragments arranged by size: 5333 457 391 SalI G'TCGA_C Cuts at: 1936 3523 1936 Size: 1587 4594 SanDI GG'GwC_CC Cuts at: 3022 3022 Size: 6181 SapI GCTCTTCn'nnn_ Cuts at: 2469 2469 Size: 6181 SbfI CC_TGCA'GG Cuts at: 3533 3533 Size: 6181 ScaI AGT'ACT Cuts at: 4429 6141 4429 Size: 1712 4469 SfcI C'TryA_G Cuts at: 826 1045 2107 2882 2950 3277 3435 3529 4664 Size: 219 1062 775 68 327 158 94 1135 Cuts at: 4664 5345 5536 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1062 775 681 327 219 191 158 94 68 SfoI GGC'GCC Cuts at: 2695 2695 Size: 6181 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 3508 1872 Size: 1636 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2121 4285 5186 5448 820 Size: 197 926 178 2164 901 262 1553 Fragments arranged by size: 2164 1553 926 901 262 197 178 SnaBI TAC'GTA Cuts at: 493 3478 3972 493 Size: 2985 494 2702 Fragments arranged by size: 2985 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 3539 1132 Size: 2407 3774 SspI AAT'ATT Cuts at: 5 52 4105 5 Size: 47 4053 2081 Fragments arranged by size: 4053 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6181 StyI C'CwwG_G Cuts at: 513 1065 2022 2387 3620 513 Size: 552 957 365 1233 3074 Fragments arranged by size: 3074 1233 957 552 365 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2342 2353 4208 4225 4378 4563 5008 Size: 1255 762 11 1855 17 153 185 445 Cuts at: 5008 325 Size: 1498 Fragments arranged by size: 1855 1498 1255 762 445 185 153 17 11 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4427 6139 96 Size: 274 80 33 51 1443 2450 1712 138 Fragments arranged by size: 2450 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2897 2929 6141 6173 2897 Size: 32 3212 32 2905 Fragments arranged by size: 3212 2905 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6181 XbaI T'CTAG_A Cuts at: 3517 3517 Size: 6181 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2998 3328 2998 Size: 330 5851 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6181 XmaI C'CCGG_G Cuts at: 1870 3506 1870 Size: 1636 4545 XmnI GAAnn'nnTTC Cuts at: 4310 4310 Size: 6181 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3989 276 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SrfI SwaI