Restriction map for
pFN21ASDA1724
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AflII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AvaII
AviII BalI BbsI BcgI BfrI
BglI BglII BpmI BpuAI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsoBI Bsp1407I BspDI
BspEI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstUI BstXI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRI EcoRV FauI
FseI FspI HgaI HincII HindII
HindIII HpaI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI MwoI NaeI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AcyI 144 GGCGCC G[GA]^CG[CT]C
AflIII 910 ACATGT A^C[GA][CT]GT
AocI 356 CCTTAGG CC^T[ACGT]AGG
Asp700I 338 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 1005 CCTAGG C^CTAGG
BamHI 827 GGATCC G^GATCC
BanI 144 GGCGCC G^G[CT][GA]CC
BanII 21 GAGCCC G[GA]GC[CT]^C
BbeI 144 GGCGCC GGCGC^C
BbrPI 250 CACGTG CAC^GTG
BbvCI 230 CCTCAGC CCTCAGC(-5/-2)
BciVI 69 GGATAC GTATCC(6/5)
BclI 372 TGATCA T^GATCA
BlnI 1005 CCTAGG C^CTAGG
BlpI 1104 GCTGAGC GC^T[ACGT]AGC
BmyI 21 GAGCCC G[AGT]GC[ACT]^C
Bpu1102I 1104 GCTGAGC GC^T[ACGT]AGC
BsaBI 70 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 144 GGCGCC G[GA]^CG[CT]C
BsaI 656 GAGACC GGTCTC(1/5)
BsmAI 656 GAGAC GTCTC(1/5)
BsmFI 507 GTCCC GGGAC(10/14)
BsmI 1031 GCATTC GAATGC(1/-1)
Bsp1286I 21 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 910 ACATGT A^CATGT
BsrDI 670 GCAATG GCAATG(2/0)
Bst1107I 1041 GTATAC GTA^TAC
BstEII 836 GGTCACC G^GT[ACGT]ACC
BstPI 836 GGTCACC G^GT[ACGT]ACC
BstYI 827 GGATCC [GA]^GATC[CT]
BstZ17I 1041 GTATAC GTA^TAC
Bsu36I 356 CCTTAGG CC^T[ACGT]AGG
CelII 1104 GCTGAGC GC^T[ACGT]AGC
CfoI 145 GCGC GCG^C
Cfr13I 1068 GGCCC G^G[ACGT]CC
DsaI 547 CCATGG C^C[GA][CT]GG
Eco81I 356 CCTTAGG CC^T[ACGT]AGG
EcoO65I 836 GGTCACC G^GT[ACGT]ACC
EcoT22I 97 ATGCAT ATGCA^T
FbaI 372 TGATCA T^GATCA
HaeII 144 GGCGCC [GA]GCGC^[CT]
HhaI 145 GCGC GCG^C
Hin1I 144 GGCGCC G[GA]^CG[CT]C
HinP1I 145 GCGC G^CGC
KasI 144 GGCGCC G^GCGCC
MamI 70 GATACCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 827 GGATCC [GA]^GATC[CT]
MslI 666 CACTGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 144 GGCGCC GG^CGCC
NcoI 547 CCATGG C^CATGG
NsiI 97 ATGCAT ATGCA^T
NspI 910 ACATGT [GA]CATG^[CT]
PleI 699 GACTC GAGTC(4/5)
PmaCI 250 CACGTG CAC^GTG
PmlI 250 CACGTG CAC^GTG
PstI 599 CTGCAG CTGCA^G
Sau96I 1068 GGCCC G^G[ACGT]CC
SexAI 237 ACCAGGT A^CC[AT]GGT
SfoI 144 GGCGCC GGC^GCC
StuI 617 AGGCCT AGG^CCT
TfiI 534 GATTC G^A[AT]TC
XbaI 306 TCTAGA T^CTAGA
XhoII 827 GGATCC [GA]^GATC[CT]
XmnI 338 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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BcnI 24 CCCGG CC^[GC]GG
944 CCCGG
BsaAI 16 TACGTG [CT]AC^GT[GA]
250 CACGTG
BsaJI 547 CCATGG C^C[ACGT][ACGT]GG
1005 CCTAGG
BsiYI 474 CCTGCTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
543 CCTGCCATGGG
BslI 474 CCTGCTATGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
543 CCTGCCATGGG
EcoT14I 547 CCATGG C^C[AT][AT]GG
1005 CCTAGG
HapII 25 CCGG C^CGG
945 CCGG
HinfI 534 GATTC G^A[ACGT]TC
699 GACTC
HpaII 25 CCGG C^CGG
945 CCGG
HphI 570 GGTGA GGTGA(8/7)
838 TCACC
MspI 25 CCGG C^CGG
945 CCGG
NciI 24 CCCGG CC^[GC]GG
944 CCCGG
SfaNI 99 GCATC GCATC(5/9)
281 GCATC
StyI 547 CCATGG C^C[AT][AT]GG
1005 CCTAGG
XcmI 952 CCAACTTTGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1071 CCACATCCATTATGG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AccI AfaI AluI AlwI BbvI
BfaI BsrI BssKI BstF5I BstNI
Cac8I DdeI DpnII EarI EcoRII
Fnu4HI FokI HaeIII ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MspA1I MvaI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
ScrFI SfcI Tru9I TseI Tsp45I
Tsp509I TspRI