(Circular) (Six-base) MAPSORT of: pFN21ASDA1724.seq Check: 610 from: 1 to: 5887 pFN21ASDA1724 5887 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2610 2840 3065 3230 1937 Size: 673 230 225 165 4594 Fragments arranged by size: 4594 673 230 225 165 Acc65I G'GTAC_C Cuts at: 3208 3208 Size: 5887 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2850 3950 5334 5602 1632 Size: 1218 1100 1384 268 1917 Fragments arranged by size: 1917 1384 1218 1100 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 2933 4786 5763 1203 Size: 1730 1853 977 1327 Fragments arranged by size: 1853 1730 1327 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5887 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5887 AlwNI CAG_nnn'CTG Cuts at: 3630 5202 3630 Size: 1572 4315 ApaLI G'TGCA_C Cuts at: 3944 5100 3944 Size: 1156 4731 ApoI r'AATT_y Cuts at: 1493 3196 3422 3574 5683 1493 Size: 1703 226 152 2109 1697 Fragments arranged by size: 2109 1703 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 3212 1870 Size: 73 1269 4545 Fragments arranged by size: 4545 1269 73 AvrII C'CTAG_G Cuts at: 3028 3028 Size: 5887 BamHI G'GATC_C Cuts at: 2850 3217 3584 2850 Size: 367 367 5153 Fragments arranged by size: 5153 367 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2167 3208 4663 618 Size: 325 217 627 380 1041 1455 1842 Fragments arranged by size: 1842 1455 1041 627 380 325 217 BanII G_rGCy'C Cuts at: 1391 2048 3206 1391 Size: 657 1158 4072 Fragments arranged by size: 4072 1158 657 BbeI G_GCGC'C Cuts at: 2171 2171 Size: 5887 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4916 BbvCI CC'TCA_GC Cuts at: 2254 2254 Size: 5887 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4078 4112 1903 Size: 34 2141 34 3678 Fragments arranged by size: 3678 2141 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2086 3779 4989 2086 Size: 1693 1210 2984 Fragments arranged by size: 2984 1693 1210 BclI T'GATC_A Cuts at: 1548 2395 1548 Size: 847 5040 BfrBI ATG'CAT Cuts at: 2122 2122 Size: 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4498 136 Size: 107 122 71 631 605 2826 1525 Fragments arranged by size: 2826 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5882 5882 Size: 5887 BlpI GC'TnA_GC Cuts at: 3128 3304 3128 Size: 176 5711 Bme1580I G_kGCm'C Cuts at: 1790 3948 5104 1790 Size: 2158 1156 2573 Fragments arranged by size: 2573 2158 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5887 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2293 2545 3618 Size: 416 53 83 399 24 876 252 1073 Cuts at: 3618 4576 442 Size: 958 1753 Fragments arranged by size: 1753 1073 958 876 416 399 252 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4547 1239 Size: 3308 2579 Bpu10I CC'TnA_GC Cuts at: 2254 2254 Size: 5887 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4012 4877 5175 4012 Size: 865 298 4724 Fragments arranged by size: 4724 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2673 4550 882 Size: 617 1174 1877 2219 Fragments arranged by size: 2219 1877 1174 617 BsaAI yAC'GTr Cuts at: 493 1204 2041 2275 3184 3678 493 Size: 711 837 234 909 494 2702 Fragments arranged by size: 2702 909 837 711 494 234 BsaBI GATnn'nnATC Cuts at: 2097 5881 2097 Size: 3784 2103 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2168 3694 4076 4711 Size: 53 83 186 936 635 1526 382 635 Cuts at: 4711 275 Size: 1451 Fragments arranged by size: 1526 1451 936 635 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4320 4718 4992 5139 5550 4320 Size: 398 274 147 411 4657 Fragments arranged by size: 4657 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3099 3129 1178 Size: 30 165 30 1696 30 3936 Fragments arranged by size: 3936 1696 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5887 BseYI C'CCAG_C Cuts at: 5090 5586 5090 Size: 496 5391 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5314 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3337 4098 5126 1540 Size: 275 186 1336 761 1028 2301 Fragments arranged by size: 2301 1336 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3948 4033 5104 3948 Size: 85 1071 4731 Fragments arranged by size: 4731 1071 85 BsmI GAATG_Cn' Cuts at: 3054 3398 3491 3054 Size: 344 93 5450 Fragments arranged by size: 5450 344 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2048 3206 3948 4033 5104 1391 Size: 399 258 1158 742 85 1071 2174 Fragments arranged by size: 2174 1158 1071 742 399 258 85 BspEI T'CCGG_A Cuts at: 5550 5550 Size: 5887 BspHI T'CATG_A Cuts at: 3776 5506 3776 Size: 1730 4157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3242 844 Size: 2398 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3774 1394 Size: 429 1951 3507 Fragments arranged by size: 3507 1951 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2700 4382 4556 65 Size: 1183 1452 1682 174 1396 Fragments arranged by size: 1682 1452 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4531 4718 1700 Size: 110 2721 187 2869 Fragments arranged by size: 2869 2721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5887 BssSI C'ACGA_G Cuts at: 3947 4959 3947 Size: 1012 4875 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5887 BstBI TT'CG_AA Cuts at: 2007 4691 2007 Size: 2684 3203 BstEII G'GTnAC_C Cuts at: 1184 2859 1184 Size: 1675 4212 BstYI r'GATC_y Cuts at: 1972 2850 3217 3584 3969 3986 5438 5524 5536 Size: 878 367 367 385 17 1452 86 12 Cuts at: 5536 5882 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 878 385 367 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3066 3066 Size: 5887 Bsu36I CC'TnA_GG Cuts at: 2380 2380 Size: 5887 BtgI C'CryG_G Cuts at: 513 1065 2022 2570 513 Size: 552 957 548 3830 Fragments arranged by size: 3830 957 552 548 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5148 Fragments arranged by size: 5148 683 56 BtsI GCAGTG_nn' Cuts at: 2688 3403 4197 4217 2688 Size: 715 794 20 4358 Fragments arranged by size: 4358 794 715 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5887 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5852 DraI TTT'AAA Cuts at: 3191 3191 Size: 5887 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4894 809 Size: 507 3578 1802 Fragments arranged by size: 3578 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3334 4223 5852 Size: 54 1425 50 275 18 1504 889 1629 Cuts at: 5852 8 Size: 43 Fragments arranged by size: 1629 1504 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2452 2730 3008 3817 1353 Size: 1099 278 278 809 3423 Fragments arranged by size: 3423 1099 809 278 278 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4474 4848 4994 387 Size: 1064 138 2885 374 146 1280 Fragments arranged by size: 2885 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2850 3950 4547 5334 5602 1239 Size: 393 1218 1100 597 787 268 1524 Fragments arranged by size: 1524 1218 1100 787 597 393 268 EcoRI G'AATT_C Cuts at: 3196 3574 3196 Size: 378 5509 FspI TGC'GCA Cuts at: 4393 4393 Size: 5887 HaeII r_GCGC'y Cuts at: 1678 2171 5034 1678 Size: 493 2863 2531 Fragments arranged by size: 2863 2531 493 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2399 2431 4528 4560 4602 Size: 32 135 32 827 32 2097 32 42 Cuts at: 4602 4634 1373 Size: 32 2626 Fragments arranged by size: 2626 2097 827 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3231 3477 669 Size: 988 281 1293 246 3079 Fragments arranged by size: 3079 1293 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5887 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KasI G'GCGC_C Cuts at: 2167 2167 Size: 5887 KpnI G_GTAC'C Cuts at: 3212 3212 Size: 5887 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2890 2999 5001 5185 1305 Size: 1585 109 2002 184 2007 Fragments arranged by size: 2007 2002 1585 184 109 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4065 Fragments arranged by size: 4065 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2693 3845 4204 4363 518 Size: 648 1527 1152 359 159 2042 Fragments arranged by size: 2042 1527 1152 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2056 2941 3127 3303 3980 5128 5373 1606 Size: 450 885 186 176 677 1148 245 2120 Fragments arranged by size: 2120 1148 885 677 450 245 186 176 NarI GG'CG_CC Cuts at: 2168 2168 Size: 5887 NcoI C'CATG_G Cuts at: 513 1065 2022 2570 513 Size: 552 957 548 3830 Fragments arranged by size: 3830 957 552 548 NdeI CA'TA_TG Cuts at: 387 387 Size: 5887 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5887 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NsiI A_TGCA'T Cuts at: 2124 2124 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 2937 3245 4790 1132 Size: 97 1708 308 1545 2229 Fragments arranged by size: 2229 1708 1545 308 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5887 PciI A'CATG_T Cuts at: 2933 4786 2933 Size: 1853 4034 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5887 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PmlI CAC'GTG Cuts at: 2275 2275 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3956 3988 5469 5501 3956 Size: 32 1481 32 4342 Fragments arranged by size: 4342 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PstI C_TGCA'G Cuts at: 830 2626 3239 830 Size: 1796 613 3478 Fragments arranged by size: 3478 1796 613 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 PvuII CAG'CTG Cuts at: 2056 2941 3127 2056 Size: 885 186 4816 Fragments arranged by size: 4816 885 186 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SbfI CC_TGCA'GG Cuts at: 3239 3239 Size: 5887 ScaI AGT'ACT Cuts at: 4135 5847 4135 Size: 1712 4175 SexAI A'CCwGG_T Cuts at: 2260 2260 Size: 5887 SfcI C'TryA_G Cuts at: 826 1045 2139 2622 2841 3235 4370 5051 5242 Size: 219 1094 483 219 394 1135 681 191 Cuts at: 5242 826 Size: 1471 Fragments arranged by size: 1471 1135 1094 681 483 394 219 219 191 SfoI GGC'GCC Cuts at: 2169 2169 Size: 5887 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3214 1872 Size: 1342 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3991 4892 5154 820 Size: 197 926 2048 901 262 1553 Fragments arranged by size: 2048 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3184 3678 493 Size: 2691 494 2702 Fragments arranged by size: 2702 2691 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 3245 1132 Size: 2113 3774 SspI AAT'ATT Cuts at: 5 52 3811 5 Size: 47 3759 2081 Fragments arranged by size: 3759 2081 47 StuI AGG'CCT Cuts at: 1517 2642 1517 Size: 1125 4762 StyI C'CwwG_G Cuts at: 513 1065 2022 2570 3028 3326 513 Size: 552 957 548 458 298 3074 Fragments arranged by size: 3074 957 552 548 458 298 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3914 3931 4084 4269 4714 325 Size: 1255 2334 17 153 185 445 1498 Fragments arranged by size: 2334 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2073 2079 2930 Size: 274 80 33 51 1443 96 6 851 Cuts at: 2930 4133 5845 96 Size: 1203 1712 138 Fragments arranged by size: 1712 1443 1203 851 274 138 96 80 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3099 3131 5847 5879 3099 Size: 32 2716 32 3107 Fragments arranged by size: 3107 2716 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5887 XbaI T'CTAG_A Cuts at: 2329 3223 2329 Size: 894 4993 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2982 3101 2982 Size: 119 5768 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5887 XmaI C'CCGG_G Cuts at: 1870 3212 1870 Size: 1342 4545 XmnI GAAnn'nnTTC Cuts at: 2365 4016 2365 Size: 1651 4236 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BplI BsiWI BsmBI BstXI DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI