Restriction map for
pFN21ASDA1724
(Linear) (Six-base) MAPSORT of: KIAA1724.seq Check: 5338 from: 1 to: 1158
KIAA1724.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AccI GT'mk_AC
Cuts at: 0 587 817 1042 1158
Size: 587 230 225 116
Fragments arranged by size:
587 230 225 116
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1158
Size: 827 331
AflIII A'CryG_T
Cuts at: 0 910 1158
Size: 910 248
AvrII C'CTAG_G
Cuts at: 0 1005 1158
Size: 1005 153
BamHI G'GATC_C
Cuts at: 0 827 1158
Size: 827 331
BanI G'GyrC_C
Cuts at: 0 144 1158
Size: 144 1014
BanII G_rGCy'C
Cuts at: 0 25 1158
Size: 25 1133
BbeI G_GCGC'C
Cuts at: 0 148 1158
Size: 148 1010
BbvCI CC'TCA_GC
Cuts at: 0 231 1158
Size: 231 927
BciVI GTATCCnnnnn_n'
Cuts at: 0 63 1158
Size: 63 1095
BclI T'GATC_A
Cuts at: 0 372 1158
Size: 372 786
BfrBI ATG'CAT
Cuts at: 0 99 1158
Size: 99 1059
BlpI GC'TnA_GC
Cuts at: 0 1105 1158
Size: 1105 53
BmrI ACTGGGnnnn_n'
Cuts at: 0 270 522 1158
Size: 270 252 636
Fragments arranged by size:
636 270 252
Bpu10I CC'TnA_GC
Cuts at: 0 231 1158
Size: 231 927
BsaI GGTCTCn'nnnn_
Cuts at: 0 650 1158
Size: 650 508
BsaAI yAC'GTr
Cuts at: 0 18 252 1158
Size: 18 234 906
Fragments arranged by size:
906 234 18
BsaBI GATnn'nnATC
Cuts at: 0 74 1158
Size: 74 1084
BsaHI Gr'CG_yC
Cuts at: 0 145 1158
Size: 145 1013
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1076 1106 1158
Size: 1076 30 52
Fragments arranged by size:
1076 52 30
BsmI GAATG_Cn'
Cuts at: 0 1031 1158
Size: 1031 127
Bsp1286I G_dGCh'C
Cuts at: 0 25 1158
Size: 25 1133
BsrDI GCAATG_nn'
Cuts at: 0 677 1158
Size: 677 481
BstEII G'GTnAC_C
Cuts at: 0 836 1158
Size: 836 322
BstYI r'GATC_y
Cuts at: 0 827 1158
Size: 827 331
BstZ17I GTA'TAC
Cuts at: 0 1043 1158
Size: 1043 115
Bsu36I CC'TnA_GG
Cuts at: 0 357 1158
Size: 357 801
BtgI C'CryG_G
Cuts at: 0 547 1158
Size: 547 611
BtsI GCAGTG_nn'
Cuts at: 0 665 1158
Size: 665 493
EarI CTCTTCn'nnn_
Cuts at: 0 429 707 985 1158
Size: 429 278 278 173
Fragments arranged by size:
429 278 278 173
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 827 1158
Size: 827 331
HaeII r_GCGC'y
Cuts at: 0 148 1158
Size: 148 1010
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 376 408 1158
Size: 376 32 750
Fragments arranged by size:
750 376 32
KasI G'GCGC_C
Cuts at: 0 144 1158
Size: 144 1014
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 867 976 1158
Size: 867 109 182
Fragments arranged by size:
867 182 109
MslI CAynn'nnrTG
Cuts at: 0 670 1158
Size: 670 488
MspA1I CmG'CkG
Cuts at: 0 33 918 1104 1158
Size: 33 885 186 54
Fragments arranged by size:
885 186 54 33
NarI GG'CG_CC
Cuts at: 0 145 1158
Size: 145 1013
NcoI C'CATG_G
Cuts at: 0 547 1158
Size: 547 611
NsiI A_TGCA'T
Cuts at: 0 101 1158
Size: 101 1057
NspI r_CATG'y
Cuts at: 0 914 1158
Size: 914 244
PciI A'CATG_T
Cuts at: 0 910 1158
Size: 910 248
PmlI CAC'GTG
Cuts at: 0 252 1158
Size: 252 906
PstI C_TGCA'G
Cuts at: 0 603 1158
Size: 603 555
PvuII CAG'CTG
Cuts at: 0 33 918 1104 1158
Size: 33 885 186 54
Fragments arranged by size:
885 186 54 33
SexAI A'CCwGG_T
Cuts at: 0 237 1158
Size: 237 921
SfcI C'TryA_G
Cuts at: 0 116 599 818 1158
Size: 116 483 219 340
Fragments arranged by size:
483 340 219 116
SfoI GGC'GCC
Cuts at: 0 146 1158
Size: 146 1012
StuI AGG'CCT
Cuts at: 0 619 1158
Size: 619 539
StyI C'CwwG_G
Cuts at: 0 547 1005 1158
Size: 547 458 153
Fragments arranged by size:
547 458 153
TatI w'GTAC_w
Cuts at: 0 50 56 907 1158
Size: 50 6 851 251
Fragments arranged by size:
851 251 50 6
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1076 1108 1158
Size: 1076 32 50
Fragments arranged by size:
1076 50 32
XbaI T'CTAG_A
Cuts at: 0 306 1158
Size: 306 852
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 959 1078 1158
Size: 959 119 80
Fragments arranged by size:
959 119 80
XmnI GAAnn'nnTTC
Cuts at: 0 342 1158
Size: 342 816
Enzymes that do cut:
AccI AcuI AflIII AvrII BamHI BanI BanII BbeI
BbvCI BciVI BclI BfrBI BlpI BmrI Bpu10I BsaI
BsaAI BsaBI BsaHI BsaXI BsmI Bsp1286I BsrDI BstEII
BstYI BstZ17I Bsu36I BtgI BtsI EarI Eco57MI HaeII
Hin4I KasI MmeI MslI MspA1I NarI NcoI NsiI
NspI PciI PmlI PstI PvuII SexAI SfcI SfoI
StuI StyI TatI TstI XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI BaeI BbsI BcgI BglI BglII
Bme1580I BmgBI BmtI BplI BpmI BpuEI BsaWI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstXI BtgZI ClaI CspCI DraI DraIII DrdI EaeI
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MscI NaeI NdeI NgoMIV NheI NotI NruI
PacI PasI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SfiI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI SwaI
TaqII Tth111I XhoI XmaI ZraI