(Circular) (Six-base) MAPSORT of: pFN21ASDA1723.seq Check: 4892 from: 1 to: 9313 pFN21ASDA1723 9313 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2777 2777 Size: 9313 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7123 278 Size: 53 83 186 936 5587 2468 Fragments arranged by size: 5587 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6204 6656 1937 Size: 4267 452 4594 Fragments arranged by size: 4594 4267 452 Acc65I G'GTAC_C Cuts at: 6634 6634 Size: 9313 AclI AA'CG_TT Cuts at: 1563 7440 7813 1563 Size: 5877 373 3063 Fragments arranged by size: 5877 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3952 3958 4064 4166 4473 4631 5344 5492 Size: 2320 6 106 102 307 158 713 148 Cuts at: 5492 5759 5948 6550 6561 7376 8760 9028 1632 Size: 267 189 602 11 815 1384 268 1917 Fragments arranged by size: 2320 1917 1384 815 713 602 307 268 267 189 158 148 106 102 11 6 AflII C'TTAA_G Cuts at: 820 1017 2617 6484 820 Size: 197 1600 3867 3649 Fragments arranged by size: 3867 3649 1600 197 AflIII A'CryG_T Cuts at: 1203 2059 6468 8212 9189 1203 Size: 856 4409 1744 977 1327 Fragments arranged by size: 4409 1744 1327 977 856 AgeI A'CCGG_T Cuts at: 8144 8144 Size: 9313 AhdI GACnn_n'nnGTC Cuts at: 3274 4017 8042 3274 Size: 743 4025 4545 Fragments arranged by size: 4545 4025 743 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 9313 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 9313 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4424 4456 4424 Size: 32 9281 AlwNI CAG_nnn'CTG Cuts at: 3212 5395 5569 5632 7056 8628 3212 Size: 2183 174 63 1424 1572 3897 Fragments arranged by size: 3897 2183 1572 1424 174 63 ApaI G_GGCC'C Cuts at: 4113 4113 Size: 9313 ApaLI G'TGCA_C Cuts at: 7370 8526 7370 Size: 1156 8157 ApoI r'AATT_y Cuts at: 1493 3256 5749 6231 6252 6622 6848 7000 9109 Size: 1763 2493 482 21 370 226 152 2109 Cuts at: 9109 1493 Size: 1697 Fragments arranged by size: 2493 2109 1763 1697 482 370 226 152 21 AseI AT'TA_AT Cuts at: 160 1032 7867 160 Size: 872 6835 1606 Fragments arranged by size: 6835 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9313 AvaI C'yCGr_G Cuts at: 1870 1943 4510 4733 5882 5951 6294 6638 1870 Size: 73 2567 223 1149 69 343 344 4545 Fragments arranged by size: 4545 2567 1149 344 343 223 73 69 BamHI G'GATC_C Cuts at: 6643 7010 6643 Size: 367 8946 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5013 5414 5955 6280 6634 Size: 325 217 627 3226 401 541 325 354 Cuts at: 6634 8089 618 Size: 1455 1842 Fragments arranged by size: 3226 1842 1455 627 541 401 354 325 325 217 BanII G_rGCy'C Cuts at: 1391 4058 4113 4676 4953 5045 5358 6632 1391 Size: 2667 55 563 277 92 313 1274 4072 Fragments arranged by size: 4072 2667 1274 563 313 277 92 55 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3255 3789 928 Size: 971 1356 534 6452 Fragments arranged by size: 6452 1356 971 534 BbvCI CC'TCA_GC Cuts at: 3140 3776 3812 3140 Size: 636 36 8641 Fragments arranged by size: 8641 636 36 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3842 3876 3980 4014 5465 5499 7504 Size: 34 1905 34 104 34 1451 34 2005 Cuts at: 7504 7538 1903 Size: 34 3678 Fragments arranged by size: 3678 2005 1905 1451 104 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2650 7205 8415 2650 Size: 4555 1210 3548 Fragments arranged by size: 4555 3548 1210 BclI T'GATC_A Cuts at: 1548 3118 4098 1548 Size: 1570 980 6763 Fragments arranged by size: 6763 1570 980 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3512 3759 5582 Size: 107 122 71 631 605 1840 247 1823 Cuts at: 5582 7924 136 Size: 2342 1525 Fragments arranged by size: 2342 1840 1823 1525 631 605 247 122 107 71 BglII A'GATC_T Cuts at: 4167 5488 5538 9308 4167 Size: 1321 50 3770 4172 Fragments arranged by size: 4172 3770 1321 50 BlpI GC'TnA_GC Cuts at: 2718 5707 6078 6730 2718 Size: 2989 371 652 5301 Fragments arranged by size: 5301 2989 652 371 Bme1580I G_kGCm'C Cuts at: 1790 4113 5199 5556 5958 6352 7374 8530 1790 Size: 2323 1086 357 402 394 1022 1156 2573 Fragments arranged by size: 2573 2323 1156 1086 1022 402 394 357 BmgBI CAC'GTC Cuts at: 1318 5458 1318 Size: 4140 5173 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3415 7044 8002 Size: 416 53 83 399 24 1998 3629 958 Cuts at: 8002 442 Size: 1753 Fragments arranged by size: 3629 1998 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 9313 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5770 5770 Size: 9313 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2615 2924 3285 3886 4555 4672 4733 5171 Size: 1376 309 361 601 669 117 61 438 Cuts at: 5171 5981 6340 7973 1239 Size: 810 359 1633 2579 Fragments arranged by size: 2579 1633 1376 810 669 601 438 361 359 309 117 61 Bpu10I CC'TnA_GC Cuts at: 3140 3776 3812 4649 5496 5645 3140 Size: 636 36 837 847 149 6808 Fragments arranged by size: 6808 847 837 636 149 36 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4034 5817 7438 8303 8601 4034 Size: 1783 1621 865 298 4746 Fragments arranged by size: 4746 1783 1621 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2984 4259 4846 7976 882 Size: 617 1485 1275 587 3130 2219 Fragments arranged by size: 3130 2219 1485 1275 617 587 BsaAI yAC'GTr Cuts at: 493 1204 4759 6610 7104 493 Size: 711 3555 1851 494 2702 Fragments arranged by size: 3555 2702 1851 711 494 BsaBI GATnn'nnATC Cuts at: 6375 9307 6375 Size: 2932 6381 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3526 7120 7502 8137 Size: 53 83 186 936 1993 3594 382 635 Cuts at: 8137 275 Size: 1451 Fragments arranged by size: 3594 1993 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4942 5238 5328 7746 8144 8418 8565 8976 4942 Size: 296 90 2418 398 274 147 411 5279 Fragments arranged by size: 5279 2418 411 398 296 274 147 90 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4303 4333 5979 6009 1178 Size: 30 165 30 2900 30 1646 30 4482 Fragments arranged by size: 4482 2900 1646 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3142 3889 4151 4231 1186 Size: 1956 747 262 80 6268 Fragments arranged by size: 6268 1956 747 262 80 BseYI C'CCAG_C Cuts at: 2049 3749 3833 3848 4090 4289 4904 5187 6048 Size: 1700 84 15 242 199 615 283 861 Cuts at: 6048 8516 9012 2049 Size: 2468 496 2350 Fragments arranged by size: 2468 2350 1700 861 615 496 283 242 199 84 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 5294 6493 6555 1163 Size: 573 3558 1199 62 3921 Fragments arranged by size: 3921 3558 1199 573 62 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4894 6379 6763 7524 8552 1540 Size: 275 186 2893 1485 384 761 1028 2301 Fragments arranged by size: 2893 2301 1485 1028 761 384 275 186 BsiHKAI G_wGCw'C Cuts at: 4058 5045 6414 7374 7459 8530 4058 Size: 987 1369 960 85 1071 4841 Fragments arranged by size: 4841 1369 1071 987 960 85 BsmI GAATG_Cn' Cuts at: 6824 6917 6824 Size: 93 9220 BsmBI CGTCTCn'nnnn_ Cuts at: 4609 4609 Size: 9313 Bsp1286I G_dGCh'C Cuts at: 1391 1790 4058 4113 4676 4953 5045 5199 5358 Size: 399 2268 55 563 277 92 154 159 Cuts at: 5358 5556 5958 6352 6414 6632 7374 7459 8530 Size: 198 402 394 62 218 742 85 1071 Cuts at: 8530 1391 Size: 2174 Fragments arranged by size: 2268 2174 1071 742 563 402 399 394 277 218 198 159 154 92 85 62 55 BspEI T'CCGG_A Cuts at: 5328 8976 5328 Size: 3648 5665 BspHI T'CATG_A Cuts at: 5205 7202 8932 5205 Size: 1997 1730 5586 Fragments arranged by size: 5586 1997 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2777 3186 5118 6668 844 Size: 1933 409 1932 1550 3489 Fragments arranged by size: 3489 1933 1932 1550 409 BsrBI CCG'CTC Cuts at: 1394 1823 7200 1394 Size: 429 5377 3507 Fragments arranged by size: 5377 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2611 2779 3925 7808 7982 65 Size: 1183 1363 168 1146 3883 174 1396 Fragments arranged by size: 3883 1396 1363 1183 1146 174 168 BsrFI r'CCGG_y Cuts at: 1700 1810 7957 8144 1700 Size: 110 6147 187 2869 Fragments arranged by size: 6147 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 9313 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9313 BssSI C'ACGA_G Cuts at: 3249 7373 8385 3249 Size: 4124 1012 4177 Fragments arranged by size: 4177 4124 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 5317 5632 794 Size: 4523 315 4475 Fragments arranged by size: 4523 4475 315 BstBI TT'CG_AA Cuts at: 2007 8117 2007 Size: 6110 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9313 BstXI CCAn_nnnn'nTGG Cuts at: 3910 4559 4912 3910 Size: 649 353 8311 Fragments arranged by size: 8311 649 353 BstYI r'GATC_y Cuts at: 1972 3209 4167 4708 4959 5488 5538 6552 6643 Size: 1237 958 541 251 529 50 1014 91 Cuts at: 6643 7010 7395 7412 8864 8950 8962 9308 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1237 1014 958 541 529 385 367 346 251 91 86 50 17 12 Bsu36I CC'TnA_GG Cuts at: 4574 4574 Size: 9313 BtgI C'CryG_G Cuts at: 513 1065 3084 3279 4203 4552 513 Size: 552 2019 195 924 349 5274 Fragments arranged by size: 5274 2019 924 552 349 195 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5664 487 Size: 683 56 4438 4136 Fragments arranged by size: 4438 4136 683 56 BtsI GCAGTG_nn' Cuts at: 2471 2658 2978 6829 7623 7643 2471 Size: 187 320 3851 794 20 4141 Fragments arranged by size: 4141 3851 794 320 187 20 ClaI AT'CG_AT Cuts at: 1554 3459 1554 Size: 1905 7408 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2256 2291 529 Size: 35 1692 35 7551 Fragments arranged by size: 7551 1692 35 35 DraI TTT'AAA Cuts at: 2406 3540 6022 6043 6617 2406 Size: 1134 2482 21 574 5102 Fragments arranged by size: 5102 2482 1134 574 21 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 8320 809 Size: 507 7004 1802 Fragments arranged by size: 7004 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2997 4561 5051 Size: 54 1425 50 275 18 1167 1564 490 Cuts at: 5051 5697 6760 7649 9278 8 Size: 646 1063 889 1629 43 Fragments arranged by size: 1629 1564 1425 1167 1063 889 646 490 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6760 1537 Size: 275 4948 4090 Fragments arranged by size: 4948 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2903 3669 4607 4941 5204 5363 5726 5735 Size: 1550 766 938 334 263 159 363 9 Cuts at: 5735 7243 1353 Size: 1508 3423 Fragments arranged by size: 3423 1550 1508 938 766 363 334 263 159 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4162 4721 7900 8274 8420 387 Size: 1064 138 2573 559 3179 374 146 1280 Fragments arranged by size: 3179 2573 1280 1064 559 374 146 138 EcoICRI GAG'CTC Cuts at: 4056 5043 4056 Size: 987 8326 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2615 2924 3285 3886 3952 3958 4064 Size: 393 983 309 361 601 66 6 106 Cuts at: 4064 4166 4473 4555 4631 4672 4733 5171 5344 Size: 102 307 82 76 41 61 438 173 Cuts at: 5344 5492 5759 5948 5981 6340 6550 6561 7376 Size: 148 267 189 33 359 210 11 815 Cuts at: 7376 7973 8760 9028 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 983 815 787 601 597 438 393 361 359 309 307 268 267 210 189 173 148 106 102 82 76 66 61 41 33 11 6 EcoNI CCTnn'n_nnAGG Cuts at: 3737 5758 3737 Size: 2021 7292 EcoO109I rG'GnC_Cy Cuts at: 3717 4305 4699 5254 6107 3717 Size: 588 394 555 853 6923 Fragments arranged by size: 6923 853 588 555 394 EcoRI G'AATT_C Cuts at: 5749 6622 7000 5749 Size: 873 378 8062 Fragments arranged by size: 8062 873 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3047 3047 Size: 9313 FspI TGC'GCA Cuts at: 7819 7819 Size: 9313 HaeII r_GCGC'y Cuts at: 1678 3787 4082 5569 5714 6175 8460 1678 Size: 2109 295 1487 145 461 2285 2531 Fragments arranged by size: 2531 2285 2109 1487 461 295 145 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3188 3220 3260 3275 3292 Size: 32 135 32 1616 32 40 15 17 Cuts at: 3292 3307 4003 4035 4045 4077 4768 4800 4808 Size: 15 696 32 10 32 691 32 8 Cuts at: 4808 4826 4840 4858 5032 5064 5377 5409 5884 Size: 18 14 18 174 32 313 32 475 Cuts at: 5884 5916 6584 6616 7954 7986 8028 8060 1373 Size: 32 668 32 1338 32 42 32 2626 Fragments arranged by size: 2626 1616 1338 696 691 668 475 313 174 135 42 40 32 32 32 32 32 32 32 32 32 32 32 32 18 18 17 15 15 14 10 8 HincII GTy'rAC Cuts at: 669 1657 1938 4377 5269 5431 6481 6657 6903 Size: 988 281 2439 892 162 1050 176 246 Cuts at: 6903 669 Size: 3079 Fragments arranged by size: 3079 2439 1050 988 892 281 246 176 162 HindIII A'AGCT_T Cuts at: 748 2614 3387 4546 5867 748 Size: 1866 773 1159 1321 4194 Fragments arranged by size: 4194 1866 1321 1159 773 HpaI GTT'AAC Cuts at: 6903 6903 Size: 9313 KpnI G_GTAC'C Cuts at: 6638 6638 Size: 9313 MfeI C'AATT_G Cuts at: 2693 6912 2693 Size: 4219 5094 MluI A'CGCG_T Cuts at: 9189 9189 Size: 9313 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4242 4251 4773 5031 6192 8427 8611 1305 Size: 2937 9 522 258 1161 2235 184 2007 Fragments arranged by size: 2937 2235 2007 1161 522 258 184 9 MscI TGG'CCA Cuts at: 10 64 1489 1832 2999 4563 10 Size: 54 1425 343 1167 1564 4760 Fragments arranged by size: 4760 1564 1425 1167 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2298 3767 4878 6467 6531 7271 7630 Size: 648 1132 1469 1111 1589 64 740 359 Cuts at: 7630 7789 518 Size: 159 2042 Fragments arranged by size: 2042 1589 1469 1132 1111 740 648 359 159 64 MspA1I CmG'CkG Cuts at: 1606 2891 4107 4774 5978 6539 6729 7406 8554 Size: 1285 1216 667 1204 561 190 677 1148 Cuts at: 8554 8799 1606 Size: 245 2120 Fragments arranged by size: 2120 1285 1216 1204 1148 677 667 561 245 190 NcoI C'CATG_G Cuts at: 513 1065 3084 4203 513 Size: 552 2019 1119 5623 Fragments arranged by size: 5623 2019 1119 552 NdeI CA'TA_TG Cuts at: 387 2439 5847 387 Size: 2052 3408 3853 Fragments arranged by size: 3853 3408 2052 NheI G'CTAG_C Cuts at: 1052 1052 Size: 9313 NotI GC'GGCC_GC Cuts at: 6760 6760 Size: 9313 NspI r_CATG'y Cuts at: 1132 1229 2063 2115 2351 2971 4345 5470 6472 Size: 97 834 52 236 620 1374 1125 1002 Cuts at: 6472 6499 6671 8216 1132 Size: 27 172 1545 2229 Fragments arranged by size: 2229 1545 1374 1125 1002 834 620 236 172 97 52 27 PasI CC'CwG_GG Cuts at: 1348 3713 5760 1348 Size: 2365 2047 4901 Fragments arranged by size: 4901 2365 2047 PciI A'CATG_T Cuts at: 2059 6468 8212 2059 Size: 4409 1744 3160 Fragments arranged by size: 4409 3160 1744 PflMI CCAn_nnn'nTGG Cuts at: 1108 2074 5095 5341 1108 Size: 966 3021 246 5080 Fragments arranged by size: 5080 3021 966 246 PfoI T'CCnGG_A Cuts at: 4962 5381 5997 6555 4962 Size: 419 616 558 7720 Fragments arranged by size: 7720 616 558 419 PmeI GTTT'AAAC Cuts at: 6617 6617 Size: 9313 PmlI CAC'GTG Cuts at: 4759 4759 Size: 9313 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5504 5536 6177 6209 7382 7414 8895 8927 5504 Size: 32 641 32 1173 32 1481 32 5890 Fragments arranged by size: 5890 1481 1173 641 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3717 4305 4699 5254 6107 3717 Size: 588 394 555 853 6923 Fragments arranged by size: 6923 853 588 555 394 PshAI GACnn'nnGTC Cuts at: 1613 2214 1613 Size: 601 8712 PsiI TTA'TAA Cuts at: 6883 6883 Size: 9313 PspOMI G'GGCC_C Cuts at: 4109 4109 Size: 9313 PstI C_TGCA'G Cuts at: 830 2862 5118 5574 5628 6665 830 Size: 2032 2256 456 54 1037 3478 Fragments arranged by size: 3478 2256 2032 1037 456 54 PvuI CG_AT'CG Cuts at: 2001 6379 2001 Size: 4378 4935 PvuII CAG'CTG Cuts at: 2891 5978 6539 2891 Size: 3087 561 5665 Fragments arranged by size: 5665 3087 561 SacI G_AGCT'C Cuts at: 4058 5045 4058 Size: 987 8326 SalI G'TCGA_C Cuts at: 1936 6655 1936 Size: 4719 4594 SanDI GG'GwC_CC Cuts at: 3717 5254 3717 Size: 1537 7776 SapI GCTCTTCn'nnn_ Cuts at: 4941 5363 4941 Size: 422 8891 SbfI CC_TGCA'GG Cuts at: 5574 6665 5574 Size: 1091 8222 ScaI AGT'ACT Cuts at: 7561 9273 7561 Size: 1712 7601 SexAI A'CCwGG_T Cuts at: 3705 5000 3705 Size: 1295 8018 SfcI C'TryA_G Cuts at: 826 1045 2028 2858 5075 5114 5534 5570 5624 Size: 219 983 830 2217 39 420 36 54 Cuts at: 5624 6661 7796 8477 8668 826 Size: 1037 1135 681 191 1471 Fragments arranged by size: 2217 1471 1135 1037 983 830 681 420 219 191 54 39 36 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9313 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9313 SmaI CCC'GGG Cuts at: 1872 6640 1872 Size: 4768 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2617 4049 5832 6294 6484 7417 Size: 197 926 674 1432 1783 462 190 933 Cuts at: 7417 8318 8580 820 Size: 901 262 1553 Fragments arranged by size: 1783 1553 1432 933 926 901 674 462 262 197 190 SnaBI TAC'GTA Cuts at: 493 6610 7104 493 Size: 6117 494 2702 Fragments arranged by size: 6117 2702 494 SpeI A'CTAG_T Cuts at: 152 2125 2341 152 Size: 1973 216 7124 Fragments arranged by size: 7124 1973 216 SphI G_CATG'C Cuts at: 1132 2971 6671 1132 Size: 1839 3700 3774 Fragments arranged by size: 3774 3700 1839 SspI AAT'ATT Cuts at: 5 52 7237 5 Size: 47 7185 2081 Fragments arranged by size: 7185 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 9313 StyI C'CwwG_G Cuts at: 513 1065 2413 2446 3084 3381 3468 3951 4203 Size: 552 1348 33 638 297 87 483 252 Cuts at: 4203 4602 5556 6752 513 Size: 399 954 1196 3074 Fragments arranged by size: 3074 1348 1196 954 638 552 483 399 297 252 87 33 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2653 3017 4828 4908 4918 6383 7340 Size: 1255 1073 364 1811 80 10 1465 957 Cuts at: 7340 7357 7510 7695 8140 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1811 1498 1465 1255 1073 957 445 364 185 153 80 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2779 4344 7559 Size: 274 80 33 51 1443 802 1565 3215 Cuts at: 7559 9271 96 Size: 1712 138 Fragments arranged by size: 3215 1712 1565 1443 802 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3574 3606 4303 4335 9273 9305 3574 Size: 32 697 32 4938 32 3582 Fragments arranged by size: 4938 3582 697 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3707 4838 5426 1316 Size: 2391 1131 588 5203 Fragments arranged by size: 5203 2391 1131 588 XbaI T'CTAG_A Cuts at: 6649 6649 Size: 9313 XcmI CCAnnnn_n'nnnnTGG Cuts at: 5836 5836 Size: 9313 XhoI C'TCGA_G Cuts at: 1943 6294 1943 Size: 4351 4962 XmaI C'CCGG_G Cuts at: 1870 6638 1870 Size: 4768 4545 XmnI GAAnn'nnTTC Cuts at: 4975 5529 5870 7442 4975 Size: 554 341 1572 6846 Fragments arranged by size: 6846 1572 554 341 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7121 276 Size: 53 83 186 936 5587 2468 Fragments arranged by size: 5587 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI