(Circular) (Six-base) MAPSORT of: pFN21ASDA1722.seq Check: 7615 from: 1 to: 9226 pFN21ASDA1722 9226 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 7036 278 Size: 53 83 186 936 5500 2468 Fragments arranged by size: 5500 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2949 3438 6569 1937 Size: 1012 489 3131 4594 Fragments arranged by size: 4594 3131 1012 489 Acc65I G'GTAC_C Cuts at: 6547 6547 Size: 9226 AclI AA'CG_TT Cuts at: 1563 7353 7726 1563 Size: 5790 373 3063 Fragments arranged by size: 5790 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4190 4418 5530 7289 8673 8941 1632 Size: 2558 228 1112 1759 1384 268 1917 Fragments arranged by size: 2558 1917 1759 1384 1112 268 228 AflII C'TTAA_G Cuts at: 820 1017 2674 4720 6092 820 Size: 197 1657 2046 1372 3954 Fragments arranged by size: 3954 2046 1657 1372 197 AflIII A'CryG_T Cuts at: 1203 8125 9102 1203 Size: 6922 977 1327 Fragments arranged by size: 6922 1327 977 AgeI A'CCGG_T Cuts at: 8057 8057 Size: 9226 AhdI GACnn_n'nnGTC Cuts at: 7955 7955 Size: 9226 AleI CACnn'nnGTG Cuts at: 1166 2750 1166 Size: 1584 7642 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2375 2768 5504 1184 Size: 1191 393 2736 4906 Fragments arranged by size: 4906 2736 1191 393 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6291 6323 6291 Size: 32 9194 AlwNI CAG_nnn'CTG Cuts at: 3424 6484 6969 8541 3424 Size: 3060 485 1572 4109 Fragments arranged by size: 4109 3060 1572 485 ApaI G_GGCC'C Cuts at: 5577 5577 Size: 9226 ApaLI G'TGCA_C Cuts at: 7283 8439 7283 Size: 1156 8070 ApoI r'AATT_y Cuts at: 1493 2052 2315 3146 3494 3784 6110 6535 6761 Size: 559 263 831 348 290 2326 425 226 Cuts at: 6761 6913 9022 1493 Size: 152 2109 1697 Fragments arranged by size: 2326 2109 1697 831 559 425 348 290 263 226 152 AseI AT'TA_AT Cuts at: 160 1032 4121 4260 7780 160 Size: 872 3089 139 3520 1606 Fragments arranged by size: 3520 3089 1606 872 139 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 9226 AvaI C'yCGr_G Cuts at: 1870 1943 2155 3849 4935 6551 1870 Size: 73 212 1694 1086 1616 4545 Fragments arranged by size: 4545 1694 1616 1086 212 73 BamHI G'GATC_C Cuts at: 6556 6923 6556 Size: 367 8859 BanI G'GyrC_C Cuts at: 618 943 1160 1787 5767 5978 6359 6547 8002 Size: 325 217 627 3980 211 381 188 1455 Cuts at: 8002 618 Size: 1842 Fragments arranged by size: 3980 1842 1455 627 381 325 217 211 188 BanII G_rGCy'C Cuts at: 1391 3877 5577 6354 6545 1391 Size: 2486 1700 777 191 4072 Fragments arranged by size: 4072 2486 1700 777 191 BbeI G_GCGC'C Cuts at: 6363 6363 Size: 9226 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 5022 5229 5661 6127 928 Size: 971 3123 207 432 466 4027 Fragments arranged by size: 4027 3123 971 466 432 207 BbvCI CC'TCA_GC Cuts at: 2195 2707 3290 2195 Size: 512 583 8131 Fragments arranged by size: 8131 583 512 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2128 2162 4874 4908 7417 7451 1903 Size: 34 191 34 2712 34 2509 34 3678 Fragments arranged by size: 3678 2712 2509 191 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3238 7118 8328 3238 Size: 3880 1210 4136 Fragments arranged by size: 4136 3880 1210 BclI T'GATC_A Cuts at: 1548 2326 5920 1548 Size: 778 3594 4854 Fragments arranged by size: 4854 3594 778 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4622 7837 136 Size: 107 122 71 631 605 2950 3215 1525 Fragments arranged by size: 3215 2950 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4818 9221 4818 Size: 4403 4823 BlpI GC'TnA_GC Cuts at: 3552 6643 3552 Size: 3091 6135 Bme1580I G_kGCm'C Cuts at: 1790 5346 5577 5772 6320 7287 8443 1790 Size: 3556 231 195 548 967 1156 2573 Fragments arranged by size: 3556 2573 1156 967 548 231 195 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 9226 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2257 5392 6460 Size: 416 53 83 399 24 840 3135 1068 Cuts at: 6460 6957 7915 442 Size: 497 958 1753 Fragments arranged by size: 3135 1753 1068 958 840 497 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 4200 4341 1056 Size: 3144 141 5941 Fragments arranged by size: 5941 3144 141 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6226 6226 Size: 9226 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2291 2465 2675 2913 3362 3446 3560 5090 Size: 1052 174 210 238 449 84 114 1530 Cuts at: 5090 5775 7886 1239 Size: 685 2111 2579 Fragments arranged by size: 2579 2111 1530 1052 685 449 238 210 174 114 84 Bpu10I CC'TnA_GC Cuts at: 2195 2707 3104 3290 2195 Size: 512 397 186 8131 Fragments arranged by size: 8131 512 397 186 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2540 2795 2990 4743 4835 7351 8216 8514 2540 Size: 255 195 1753 92 2516 865 298 3252 Fragments arranged by size: 3252 2516 1753 865 298 255 195 92 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2696 3170 6232 7889 882 Size: 617 1197 474 3062 1657 2219 Fragments arranged by size: 3062 2219 1657 1197 617 474 BsaAI yAC'GTr Cuts at: 493 1204 4634 6523 7017 493 Size: 711 3430 1889 494 2702 Fragments arranged by size: 3430 2702 1889 711 494 BsaBI GATnn'nnATC Cuts at: 4362 9220 4362 Size: 4858 4368 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3980 6261 6360 7033 Size: 53 83 186 936 2447 2281 99 673 Cuts at: 7033 7415 8050 275 Size: 382 635 1451 Fragments arranged by size: 2447 2281 1451 936 673 635 382 186 99 83 53 BsaWI w'CCGG_w Cuts at: 3771 5535 5580 7659 8057 8331 8478 8889 3771 Size: 1764 45 2079 398 274 147 411 4108 Fragments arranged by size: 4108 2079 1764 411 398 274 147 45 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2171 2201 2205 2235 3612 Size: 30 165 30 768 30 4 30 1377 Cuts at: 3612 3642 4532 4562 5321 5351 6065 6095 1178 Size: 30 890 30 759 30 714 30 4309 Fragments arranged by size: 4309 1377 890 768 759 714 165 30 30 30 30 30 30 30 30 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4155 4763 5606 5680 1186 Size: 2969 608 843 74 4732 Fragments arranged by size: 4732 2969 843 608 74 BseYI C'CCAG_C Cuts at: 2437 3557 3701 3709 4078 4803 5515 5569 6368 Size: 1120 144 8 369 725 712 54 799 Cuts at: 6368 6495 8429 8925 2437 Size: 127 1934 496 2738 Fragments arranged by size: 2738 1934 1120 799 725 712 496 369 144 127 54 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4428 4490 4673 1163 Size: 573 2692 62 183 5716 Fragments arranged by size: 5716 2692 573 183 62 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3764 6070 6676 7437 8465 1540 Size: 275 186 1763 2306 606 761 1028 2301 Fragments arranged by size: 2306 2301 1763 1028 761 606 275 186 BsiHKAI G_wGCw'C Cuts at: 3738 3877 5858 7287 7372 8443 3738 Size: 139 1981 1429 85 1071 4521 Fragments arranged by size: 4521 1981 1429 1071 139 85 BsmI GAATG_Cn' Cuts at: 6737 6830 6737 Size: 93 9133 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3738 3877 5346 5577 5772 5858 6320 Size: 399 1948 139 1469 231 195 86 462 Cuts at: 6320 6354 6545 7287 7372 8443 1391 Size: 34 191 742 85 1071 2174 Fragments arranged by size: 2174 1948 1469 1071 742 462 399 231 195 191 139 86 85 34 BspEI T'CCGG_A Cuts at: 5580 8889 5580 Size: 3309 5917 BspHI T'CATG_A Cuts at: 6192 7115 8845 6192 Size: 923 1730 6573 Fragments arranged by size: 6573 1730 923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4974 6581 844 Size: 4130 1607 3489 Fragments arranged by size: 4130 3489 1607 BsrBI CCG'CTC Cuts at: 1394 1823 2265 2644 4951 7113 1394 Size: 429 442 379 2307 2162 3507 Fragments arranged by size: 3507 2307 2162 442 429 379 BsrDI GCAATG_nn' Cuts at: 65 1248 7721 7895 65 Size: 1183 6473 174 1396 Fragments arranged by size: 6473 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4083 4569 7870 8057 1700 Size: 110 2273 486 3301 187 2869 Fragments arranged by size: 3301 2869 2273 486 187 110 BsrGI T'GTAC_A Cuts at: 96 3881 5493 96 Size: 3785 1612 3829 Fragments arranged by size: 3829 3785 1612 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 9226 BssSI C'ACGA_G Cuts at: 7286 8298 7286 Size: 1012 8214 BstAPI GCAn_nnn'nTGC Cuts at: 794 6082 6484 794 Size: 5288 402 3536 Fragments arranged by size: 5288 3536 402 BstBI TT'CG_AA Cuts at: 2007 8030 2007 Size: 6023 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 9226 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2611 5216 2029 Size: 582 2605 6039 Fragments arranged by size: 6039 2605 582 BstYI r'GATC_y Cuts at: 1972 2714 4396 4818 5185 6556 6923 7308 7325 Size: 742 1682 422 367 1371 367 385 17 Cuts at: 7325 8777 8863 8875 9221 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1682 1452 1371 742 422 385 367 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3439 3439 Size: 9226 Bsu36I CC'TnA_GG Cuts at: 3932 5256 5687 3932 Size: 1324 431 7471 Fragments arranged by size: 7471 1324 431 BtgI C'CryG_G Cuts at: 513 1065 3182 3997 5292 5660 5970 6384 513 Size: 552 2117 815 1295 368 310 414 3355 Fragments arranged by size: 3355 2117 1295 815 552 414 368 310 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 8487 Fragments arranged by size: 8487 683 56 BtsI GCAGTG_nn' Cuts at: 5024 6320 6482 6742 7536 7556 5024 Size: 1296 162 260 794 20 6694 Fragments arranged by size: 6694 1296 794 260 162 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 9226 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 9191 DraI TTT'AAA Cuts at: 3331 3613 4837 6530 3331 Size: 282 1224 1693 6027 Fragments arranged by size: 6027 1693 1224 282 DraIII CAC_nnn'GTG Cuts at: 2302 2302 Size: 9226 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5630 6017 8233 809 Size: 507 4314 387 2216 1802 Fragments arranged by size: 4314 2216 1802 507 387 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3761 3858 5723 Size: 54 1425 50 275 18 1931 97 1865 Cuts at: 5723 6673 7562 9191 8 Size: 950 889 1629 43 Fragments arranged by size: 1931 1865 1629 1425 950 889 275 97 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3761 6673 1537 Size: 275 1949 2912 4090 Fragments arranged by size: 4090 2912 1949 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3168 3375 4055 4911 5981 7156 1353 Size: 1815 207 680 856 1070 1175 3423 Fragments arranged by size: 3423 1815 1175 1070 856 680 207 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5127 7813 8187 8333 387 Size: 1064 138 3538 2686 374 146 1280 Fragments arranged by size: 3538 2686 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3875 3875 Size: 9226 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2465 2675 2913 3362 3446 3560 Size: 393 659 174 210 238 449 84 114 Cuts at: 3560 4190 4418 5090 5530 5775 7289 7886 8673 Size: 630 228 672 440 245 1514 597 787 Cuts at: 8673 8941 1239 Size: 268 1524 Fragments arranged by size: 1524 1514 787 672 659 630 597 449 440 393 268 245 238 228 210 174 114 84 EcoNI CCTnn'n_nnAGG Cuts at: 6095 6161 6095 Size: 66 9160 EcoO109I rG'GnC_Cy Cuts at: 5573 5573 Size: 9226 EcoRI G'AATT_C Cuts at: 2052 3146 6535 6913 2052 Size: 1094 3389 378 4365 Fragments arranged by size: 4365 3389 1094 378 EcoRV GAT'ATC Cuts at: 4987 4987 Size: 9226 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3860 4330 3860 Size: 470 8756 FspI TGC'GCA Cuts at: 7732 7732 Size: 9226 HaeII r_GCGC'y Cuts at: 1678 4698 6363 8373 1678 Size: 3020 1665 2010 2531 Fragments arranged by size: 3020 2531 2010 1665 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2171 2203 3733 3765 3859 Size: 32 135 32 599 32 1530 32 94 Cuts at: 3859 3891 4935 4967 5072 5104 5429 5461 5588 Size: 32 1044 32 105 32 325 32 127 Cuts at: 5588 5620 5887 5919 7867 7899 7941 7973 1373 Size: 32 267 32 1948 32 42 32 2626 Fragments arranged by size: 2626 1948 1530 1044 599 325 267 135 127 105 94 42 32 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4132 4187 6570 6816 669 Size: 988 281 2194 55 2383 246 3079 Fragments arranged by size: 3079 2383 2194 988 281 246 55 HindIII A'AGCT_T Cuts at: 748 2393 748 Size: 1645 7581 HpaI GTT'AAC Cuts at: 4132 6816 4132 Size: 2684 6542 KasI G'GCGC_C Cuts at: 6359 6359 Size: 9226 KpnI G_GTAC'C Cuts at: 6551 6551 Size: 9226 MfeI C'AATT_G Cuts at: 6825 6825 Size: 9226 MluI A'CGCG_T Cuts at: 9102 9102 Size: 9226 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5427 8340 8524 1305 Size: 4122 2913 184 2007 Fragments arranged by size: 4122 2913 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3860 10 Size: 54 1425 343 2028 5376 Fragments arranged by size: 5376 2028 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 2072 2609 2750 5214 6233 7184 Size: 648 861 45 537 141 2464 1019 951 Cuts at: 7184 7543 7702 518 Size: 359 159 2042 Fragments arranged by size: 2464 2042 1019 951 861 648 537 359 159 141 45 MspA1I CmG'CkG Cuts at: 1606 2405 2437 3705 5412 5662 5880 6642 7319 Size: 799 32 1268 1707 250 218 762 677 Cuts at: 7319 8467 8712 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1707 1268 1148 799 762 677 250 245 218 32 NaeI GCC'GGC Cuts at: 4085 4085 Size: 9226 NarI GG'CG_CC Cuts at: 6360 6360 Size: 9226 NcoI C'CATG_G Cuts at: 513 1065 3182 5292 513 Size: 552 2117 2110 4447 Fragments arranged by size: 4447 2117 2110 552 NdeI CA'TA_TG Cuts at: 387 4878 387 Size: 4491 4735 NgoMIV G'CCGG_C Cuts at: 4083 4083 Size: 9226 NheI G'CTAG_C Cuts at: 1052 4196 4337 1052 Size: 3144 141 5941 Fragments arranged by size: 5941 3144 141 NotI GC'GGCC_GC Cuts at: 6673 6673 Size: 9226 NspI r_CATG'y Cuts at: 1132 1229 3699 6034 6089 6584 8129 1132 Size: 97 2470 2335 55 495 1545 2229 Fragments arranged by size: 2470 2335 2229 1545 495 97 55 PasI CC'CwG_GG Cuts at: 1348 5608 1348 Size: 4260 4966 PciI A'CATG_T Cuts at: 8125 8125 Size: 9226 PflMI CCAn_nnn'nTGG Cuts at: 1108 2698 3424 5935 1108 Size: 1590 726 2511 4399 Fragments arranged by size: 4399 2511 1590 726 PfoI T'CCnGG_A Cuts at: 3364 4296 4707 3364 Size: 932 411 7883 Fragments arranged by size: 7883 932 411 PmeI GTTT'AAAC Cuts at: 6530 6530 Size: 9226 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7295 7327 8808 8840 7295 Size: 32 1481 32 7681 Fragments arranged by size: 7681 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 9226 PsiI TTA'TAA Cuts at: 5616 6796 5616 Size: 1180 8046 PspOMI G'GGCC_C Cuts at: 5573 5573 Size: 9226 PstI C_TGCA'G Cuts at: 830 2364 3259 4971 5910 6578 830 Size: 1534 895 1712 939 668 3478 Fragments arranged by size: 3478 1712 1534 939 895 668 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 9226 PvuII CAG'CTG Cuts at: 2405 2437 3705 2405 Size: 32 1268 7926 Fragments arranged by size: 7926 1268 32 SacI G_AGCT'C Cuts at: 3877 3877 Size: 9226 SacII CC_GC'GG Cuts at: 5663 5663 Size: 9226 SalI G'TCGA_C Cuts at: 1936 6568 1936 Size: 4632 4594 SapI GCTCTTCn'nnn_ Cuts at: 5981 5981 Size: 9226 SbfI CC_TGCA'GG Cuts at: 4971 6578 4971 Size: 1607 7619 ScaI AGT'ACT Cuts at: 7474 9186 7474 Size: 1712 7514 SexAI A'CCwGG_T Cuts at: 4320 4320 Size: 9226 SfcI C'TryA_G Cuts at: 826 1045 2360 2835 2987 3255 4698 4792 4903 Size: 219 1315 475 152 268 1443 94 111 Cuts at: 4903 4967 5906 6574 7709 8390 8581 826 Size: 64 939 668 1135 681 191 1471 Fragments arranged by size: 1471 1443 1315 1135 939 681 668 475 268 219 191 152 111 94 64 SfoI GGC'GCC Cuts at: 6361 6361 Size: 9226 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 9226 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 9226 SmaI CCC'GGG Cuts at: 1872 4937 6553 1872 Size: 3065 1616 4545 Fragments arranged by size: 4545 3065 1616 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 2674 2810 3005 4720 4758 Size: 197 926 612 119 136 195 1715 38 Cuts at: 4758 4814 6092 7330 8231 8493 820 Size: 56 1278 1238 901 262 1553 Fragments arranged by size: 1715 1553 1278 1238 926 901 612 262 197 195 136 119 56 38 SnaBI TAC'GTA Cuts at: 493 6523 7017 493 Size: 6030 494 2702 Fragments arranged by size: 6030 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 9226 SphI G_CATG'C Cuts at: 1132 6089 6584 1132 Size: 4957 495 3774 Fragments arranged by size: 4957 3774 495 SspI AAT'ATT Cuts at: 5 52 3518 5674 7150 5 Size: 47 3466 2156 1476 2081 Fragments arranged by size: 3466 2156 2081 1476 47 StuI AGG'CCT Cuts at: 1517 2924 4616 4901 1517 Size: 1407 1692 285 5842 Fragments arranged by size: 5842 1692 1407 285 StyI C'CwwG_G Cuts at: 513 1065 3182 3374 5203 5292 5312 5359 6665 Size: 552 2117 192 1829 89 20 47 1306 Cuts at: 6665 513 Size: 3074 Fragments arranged by size: 3074 2117 1829 1306 552 192 89 47 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2108 3563 3696 4842 5372 6472 7253 Size: 1255 528 1455 133 1146 530 1100 781 Cuts at: 7253 7270 7423 7608 8053 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1498 1455 1255 1146 1100 781 530 528 445 185 153 133 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2418 3881 5493 Size: 274 80 33 51 1443 441 1463 1612 Cuts at: 5493 5832 7472 9184 96 Size: 339 1640 1712 138 Fragments arranged by size: 1712 1640 1612 1463 1443 441 339 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2205 2237 9186 9218 2205 Size: 32 6949 32 2213 Fragments arranged by size: 6949 2213 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2945 1316 Size: 1629 7597 XbaI T'CTAG_A Cuts at: 3085 5449 6562 3085 Size: 2364 1113 5749 Fragments arranged by size: 5749 2364 1113 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2117 2725 5299 5548 2117 Size: 608 2574 249 5795 Fragments arranged by size: 5795 2574 608 249 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 9226 XmaI C'CCGG_G Cuts at: 1870 4935 6551 1870 Size: 3065 1616 4545 Fragments arranged by size: 4545 3065 1616 XmnI GAAnn'nnTTC Cuts at: 3225 3495 3971 7355 3225 Size: 270 476 3384 5096 Fragments arranged by size: 5096 3384 476 270 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 7034 276 Size: 53 83 186 936 5500 2468 Fragments arranged by size: 5500 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BfrBI BsiWI BsmBI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SanDI SfiI SrfI SwaI