Restriction map for
pFN21ASDA1716
(Linear) (Six-base) MAPSORT of: KIAA1716.seq Check: 885 from: 1 to: 2277
KIAA1716.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2225 2277
Size: 2225 52
AccI GT'mk_AC
Cuts at: 0 770 2277
Size: 770 1507
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2062 2277
Size: 2062 215
AfeI AGC'GCT
Cuts at: 0 1305 2277
Size: 1305 972
AleI CACnn'nnGTG
Cuts at: 0 802 2277
Size: 802 1475
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1582 1614 2212 2244 2277
Size: 1582 32 598 32 33
Fragments arranged by size:
1582 598 33 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1675 2277
Size: 1675 602
ApaI G_GGCC'C
Cuts at: 0 342 2277
Size: 342 1935
AvaI C'yCGr_G
Cuts at: 0 300 1058 1776 2193 2277
Size: 300 758 718 417 84
Fragments arranged by size:
758 718 417 300 84
BanI G'GyrC_C
Cuts at: 0 305 1434 1443 1582 1986 2197 2277
Size: 305 1129 9 139 404 211 80
Fragments arranged by size:
1129 404 305 211 139 80 9
BanII G_rGCy'C
Cuts at: 0 342 982 1547 1784 1918 2225 2277
Size: 342 640 565 237 134 307 52
Fragments arranged by size:
640 565 342 307 237 134 52
BbeI G_GCGC'C
Cuts at: 0 1438 1990 2201 2277
Size: 1438 552 211 76
Fragments arranged by size:
1438 552 211 76
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 24 58 1801 1835 2277
Size: 24 34 1743 34 442
Fragments arranged by size:
1743 442 34 34 24
BciVI GTATCCnnnnn_n'
Cuts at: 0 2251 2277
Size: 2251 26
BglI GCCn_nnn'nGGC
Cuts at: 0 16 337 1127 1456 1672 1846 2021 2277
Size: 16 321 790 329 216 174 175 256
Fragments arranged by size:
790 329 321 256 216 175 174 16
BglII A'GATC_T
Cuts at: 0 1316 2277
Size: 1316 961
Bme1580I G_kGCm'C
Cuts at: 0 342 1585 1809 2277
Size: 342 1243 224 468
Fragments arranged by size:
1243 468 342 224
BmgBI CAC'GTC
Cuts at: 0 660 1761 1959 2277
Size: 660 1101 198 318
Fragments arranged by size:
1101 660 318 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 456 665 1323 1869 2277
Size: 456 209 658 546 408
Fragments arranged by size:
658 546 456 408 209
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1630 1653 2277
Size: 1630 23 624
Fragments arranged by size:
1630 624 23
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 289 376 1474 1491 2209 2277
Size: 178 111 87 1098 17 718 68
Fragments arranged by size:
1098 718 178 111 87 68 17
Bpu10I CC'TnA_GC
Cuts at: 0 1268 2277
Size: 1268 1009
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 1561 2277
Size: 769 792 716
Fragments arranged by size:
792 769 716
BsaAI yAC'GTr
Cuts at: 0 1410 2277
Size: 1410 867
BsaHI Gr'CG_yC
Cuts at: 0 53 1435 1888 1987 2198 2277
Size: 53 1382 453 99 211 79
Fragments arranged by size:
1382 453 211 99 79 53
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1203 1233 1943 1973 2212 2242 2277
Size: 1203 30 710 30 239 30 35
Fragments arranged by size:
1203 710 239 35 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1717 2277
Size: 1717 560
BseYI C'CCAG_C
Cuts at: 0 68 1154 2010 2277
Size: 68 1086 856 267
Fragments arranged by size:
1086 856 267 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 967 1120 1361 1498 1712 1756 1918
Size: 481 486 153 241 137 214 44 162
Cuts at: 1918 1978 2125 2277
Size: 60 147 152
Fragments arranged by size:
486 481 241 214 162 153 152 147
137 60 44
BsiHKAI G_wGCw'C
Cuts at: 0 392 829 1198 2225 2277
Size: 392 437 369 1027 52
Fragments arranged by size:
1027 437 392 369 52
Bsp1286I G_dGCh'C
Cuts at: 0 342 392 829 982 1198 1547 1585 1784
Size: 342 50 437 153 216 349 38 199
Cuts at: 1784 1809 1918 2225 2277
Size: 25 109 307 52
Fragments arranged by size:
437 349 342 307 216 199 153 109
52 50 38 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2225 2277
Size: 2225 52
BsrBI CCG'CTC
Cuts at: 0 50 855 1567 1954 2277
Size: 50 805 712 387 323
Fragments arranged by size:
805 712 387 323 50
BsrFI r'CCGG_y
Cuts at: 0 10 308 734 1111 2020 2277
Size: 10 298 426 377 909 257
Fragments arranged by size:
909 426 377 298 257 10
BssHII G'CGCG_C
Cuts at: 0 2146 2277
Size: 2146 131
BssSI C'ACGA_G
Cuts at: 0 316 1029 1257 2277
Size: 316 713 228 1020
Fragments arranged by size:
1020 713 316 228
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1098 1193 1804 1905 2277
Size: 1098 95 611 101 372
Fragments arranged by size:
1098 611 372 101 95
BstXI CCAn_nnnn'nTGG
Cuts at: 0 678 1469 2010 2277
Size: 678 791 541 267
Fragments arranged by size:
791 678 541 267
BstYI r'GATC_y
Cuts at: 0 428 1316 2277
Size: 428 888 961
Fragments arranged by size:
961 888 428
BtgI C'CryG_G
Cuts at: 0 800 1439 1842 2216 2277
Size: 800 639 403 374 61
Fragments arranged by size:
800 639 403 374 61
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 942 2082 2277
Size: 942 1140 195
Fragments arranged by size:
1140 942 195
BtsI GCAGTG_nn'
Cuts at: 0 79 1224 1517 2108 2131 2277
Size: 79 1145 293 591 23 146
Fragments arranged by size:
1145 591 293 146 79 23
DraIII CAC_nnn'GTG
Cuts at: 0 321 2277
Size: 321 1956
EaeI y'GGCC_r
Cuts at: 0 1109 1137 1562 2022 2093 2111 2277
Size: 1109 28 425 460 71 18 166
Fragments arranged by size:
1109 460 425 166 71 28 18
EarI CTCTTCn'nnn_
Cuts at: 0 703 1621 1648 2264 2277
Size: 703 918 27 616 13
Fragments arranged by size:
918 703 616 27 13
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 355 808 954 1035 1591 1771 1879 2170
Size: 355 453 146 81 556 180 108 291
Cuts at: 2170 2277
Size: 107
Fragments arranged by size:
556 453 355 291 180 146 108 107
81
EcoICRI GAG'CTC
Cuts at: 0 2223 2277
Size: 2223 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 178 289 376 1474 1491 2062 2209 2277
Size: 178 111 87 1098 17 571 147 68
Fragments arranged by size:
1098 571 178 147 111 87 68 17
EcoNI CCTnn'n_nnAGG
Cuts at: 0 130 2277
Size: 130 2147
EcoO109I rG'GnC_Cy
Cuts at: 0 264 297 338 339 1458 1749 2085 2277
Size: 264 33 41 1 1119 291 336 192
Fragments arranged by size:
1119 336 291 264 192 41 33 1
HaeII r_GCGC'y
Cuts at: 0 741 1307 1438 1837 1990 2201 2277
Size: 741 566 131 399 153 211 76
Fragments arranged by size:
741 566 399 211 153 131 76
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 862 894 2277
Size: 862 32 1383
Fragments arranged by size:
1383 862 32
HincII GTy'rAC
Cuts at: 0 1857 2277
Size: 1857 420
KasI G'GCGC_C
Cuts at: 0 1434 1986 2197 2277
Size: 1434 552 211 80
Fragments arranged by size:
1434 552 211 80
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1679 2277
Size: 1679 598
MscI TGG'CCA
Cuts at: 0 2095 2277
Size: 2095 182
MslI CAynn'nnrTG
Cuts at: 0 802 2101 2277
Size: 802 1299 176
Fragments arranged by size:
1299 802 176
MspA1I CmG'CkG
Cuts at: 0 504 546 609 765 1154 1712 1893 2082
Size: 504 42 63 156 389 558 181 189
Cuts at: 2082 2277
Size: 195
Fragments arranged by size:
558 504 389 195 189 181 156 63
42
NaeI GCC'GGC
Cuts at: 0 1113 2277
Size: 1113 1164
NarI GG'CG_CC
Cuts at: 0 1435 1987 2198 2277
Size: 1435 552 211 79
Fragments arranged by size:
1435 552 211 79
NcoI C'CATG_G
Cuts at: 0 1439 2277
Size: 1439 838
NgoMIV G'CCGG_C
Cuts at: 0 1111 2277
Size: 1111 1166
NspI r_CATG'y
Cuts at: 0 900 2277
Size: 900 1377
PflMI CCAn_nnn'nTGG
Cuts at: 0 321 2277
Size: 321 1956
PfoI T'CCnGG_A
Cuts at: 0 431 2243 2277
Size: 431 1812 34
Fragments arranged by size:
1812 431 34
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 844 876 2212 2244 2277
Size: 844 32 1336 32 33
Fragments arranged by size:
1336 844 33 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 264 2085 2277
Size: 264 1821 192
Fragments arranged by size:
1821 264 192
PspOMI G'GGCC_C
Cuts at: 0 338 2277
Size: 338 1939
PstI C_TGCA'G
Cuts at: 0 110 410 623 905 1094 2277
Size: 110 300 213 282 189 1183
Fragments arranged by size:
1183 300 282 213 189 110
PvuII CAG'CTG
Cuts at: 0 504 546 765 2277
Size: 504 42 219 1512
Fragments arranged by size:
1512 504 219 42
SacI G_AGCT'C
Cuts at: 0 2225 2277
Size: 2225 52
SanDI GG'GwC_CC
Cuts at: 0 2085 2277
Size: 2085 192
SapI GCTCTTCn'nnn_
Cuts at: 0 1648 2277
Size: 1648 629
SfcI C'TryA_G
Cuts at: 0 106 127 406 486 619 901 993 1090
Size: 106 21 279 80 133 282 92 97
Cuts at: 1090 2277
Size: 1187
Fragments arranged by size:
1187 282 279 133 106 97 92 80
21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1846 2021 2277
Size: 1846 175 256
Fragments arranged by size:
1846 256 175
SfoI GGC'GCC
Cuts at: 0 1436 1988 2199 2277
Size: 1436 552 211 78
Fragments arranged by size:
1436 552 211 78
SmaI CCC'GGG
Cuts at: 0 1778 2195 2277
Size: 1778 417 82
Fragments arranged by size:
1778 417 82
SmlI C'TyrA_G
Cuts at: 0 784 1540 2277
Size: 784 756 737
Fragments arranged by size:
784 756 737
SphI G_CATG'C
Cuts at: 0 900 2277
Size: 900 1377
StuI AGG'CCT
Cuts at: 0 19 1390 1744 2277
Size: 19 1371 354 533
Fragments arranged by size:
1371 533 354 19
StyI C'CwwG_G
Cuts at: 0 1232 1439 2277
Size: 1232 207 838
Fragments arranged by size:
1232 838 207
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 897 1207 2277
Size: 897 310 1070
Fragments arranged by size:
1070 897 310
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 448 480 1203 1235 1943 1975 2277
Size: 448 32 723 32 708 32 302
Fragments arranged by size:
723 708 448 302 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 88 2277
Size: 88 2189
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1167 2277
Size: 1167 1110
XmaI C'CCGG_G
Cuts at: 0 1776 2193 2277
Size: 1776 417 84
Fragments arranged by size:
1776 417 84
XmnI GAAnn'nnTTC
Cuts at: 0 361 2277
Size: 361 1916
Enzymes that do cut:
AarI AccI AcuI AfeI AleI AloI AlwNI ApaI
AvaI BanI BanII BbeI BcgI BciVI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrBI BsrFI BssHII BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI
PfoI PpiI PpuMI PspOMI PstI PvuII SacI SanDI
SapI SfcI SfiI SfoI SmaI SmlI SphI StuI
StyI TaqII TstI Tth111I XcmI XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AgeI AhdI AlfI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BbvCI BclI BfrBI BlpI BmtI BsaI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrDI
BsrGI BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PshAI
PsiI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SexAI SgfI SgrAI SnaBI SpeI SrfI SspI
SwaI TatI XbaI XhoI ZraI