(Circular) (Six-base) MAPSORT of: pFN21ASDA1715.seq Check: 4182 from: 1 to: 6013 pFN21ASDA1715 6013 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2989 2989 Size: 6013 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3356 1937 Size: 1419 4594 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2697 4076 5460 5728 1632 Size: 1065 1379 1384 268 1917 Fragments arranged by size: 1917 1384 1379 1065 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 4912 5889 1203 Size: 3709 977 1327 Fragments arranged by size: 3709 1327 977 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6013 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6013 AlwNI CAG_nnn'CTG Cuts at: 2720 3251 3756 5328 2720 Size: 531 505 1572 3405 Fragments arranged by size: 3405 1572 531 505 ApaI G_GGCC'C Cuts at: 2582 2582 Size: 6013 ApaLI G'TGCA_C Cuts at: 4070 5226 4070 Size: 1156 4857 ApoI r'AATT_y Cuts at: 1493 2094 2111 2228 2891 3322 3548 3700 5809 Size: 601 17 117 663 431 226 152 2109 Cuts at: 5809 1493 Size: 1697 Fragments arranged by size: 2109 1697 663 601 431 226 152 117 17 AseI AT'TA_AT Cuts at: 160 1032 2159 2423 4567 160 Size: 872 1127 264 2144 1606 Fragments arranged by size: 2144 1606 1127 872 264 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 2772 3338 1870 Size: 73 829 566 4545 Fragments arranged by size: 4545 829 566 73 BamHI G'GATC_C Cuts at: 2699 3343 3710 2699 Size: 644 367 5002 Fragments arranged by size: 5002 644 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3334 4789 618 Size: 325 217 627 1547 1455 1842 Fragments arranged by size: 1842 1547 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2582 3332 1391 Size: 1191 750 4072 Fragments arranged by size: 4072 1191 750 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2723 928 Size: 971 824 4218 Fragments arranged by size: 4218 971 824 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4204 4238 1903 Size: 34 2267 34 3678 Fragments arranged by size: 3678 2267 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 2277 1548 Size: 729 5284 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4624 136 Size: 107 122 71 631 605 2952 1525 Fragments arranged by size: 2952 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6008 6008 Size: 6013 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 2582 4074 5230 1790 Size: 792 1492 1156 2573 Fragments arranged by size: 2573 1492 1156 792 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6013 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3744 4702 442 Size: 416 53 83 399 24 2327 958 1753 Fragments arranged by size: 2327 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3238 3238 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2305 2505 2646 2724 3273 4673 1239 Size: 1066 200 141 78 549 1400 2579 Fragments arranged by size: 2579 1400 1066 549 200 141 78 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4138 5003 5301 4138 Size: 865 298 4850 Fragments arranged by size: 4850 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4676 882 Size: 617 3177 2219 Fragments arranged by size: 3177 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3310 3804 493 Size: 711 2106 494 2702 Fragments arranged by size: 2702 2106 711 494 BsaBI GATnn'nnATC Cuts at: 6007 6007 Size: 6013 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3820 4202 4837 275 Size: 53 83 186 936 2287 382 635 1451 Fragments arranged by size: 2287 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2443 4446 4844 5118 5265 5676 2443 Size: 2003 398 274 147 411 2780 Fragments arranged by size: 2780 2003 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2478 2508 2644 2674 2751 Size: 30 165 30 1075 30 136 30 77 Cuts at: 2751 2781 1178 Size: 30 4410 Fragments arranged by size: 4410 1075 165 136 77 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2574 3206 1186 Size: 1388 632 3993 Fragments arranged by size: 3993 1388 632 BseYI C'CCAG_C Cuts at: 5216 5712 5216 Size: 496 5517 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5440 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3463 4224 5252 1540 Size: 275 186 1462 761 1028 2301 Fragments arranged by size: 2301 1462 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4074 4159 5230 4074 Size: 85 1071 4857 Fragments arranged by size: 4857 1071 85 BsmI GAATG_Cn' Cuts at: 3524 3617 3524 Size: 93 5920 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2582 3332 4074 4159 5230 1391 Size: 399 792 750 742 85 1071 2174 Fragments arranged by size: 2174 1071 792 750 742 399 85 BspEI T'CCGG_A Cuts at: 2443 5676 2443 Size: 3233 2780 BspHI T'CATG_A Cuts at: 3902 5632 3902 Size: 1730 4283 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2989 3368 844 Size: 2145 379 3489 Fragments arranged by size: 3489 2145 379 BsrBI CCG'CTC Cuts at: 1394 1823 3900 1394 Size: 429 2077 3507 Fragments arranged by size: 3507 2077 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2901 4508 4682 65 Size: 1183 1653 1607 174 1396 Fragments arranged by size: 1653 1607 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4657 4844 1700 Size: 110 2847 187 2869 Fragments arranged by size: 2869 2847 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6013 BssSI C'ACGA_G Cuts at: 4073 5085 4073 Size: 1012 5001 BstAPI GCAn_nnn'nTGC Cuts at: 794 2243 794 Size: 1449 4564 BstBI TT'CG_AA Cuts at: 2007 4817 2007 Size: 2810 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6013 BstYI r'GATC_y Cuts at: 1972 2699 3343 3710 4095 4112 5564 5650 5662 Size: 727 644 367 385 17 1452 86 12 Cuts at: 5662 6008 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 727 644 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2958 2958 Size: 6013 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4504 Fragments arranged by size: 4504 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5274 Fragments arranged by size: 5274 683 56 BtsI GCAGTG_nn' Cuts at: 2861 3529 4323 4343 2861 Size: 668 794 20 4531 Fragments arranged by size: 4531 794 668 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5978 DraI TTT'AAA Cuts at: 2358 3317 2358 Size: 959 5054 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3460 4349 5978 Size: 54 1425 50 275 18 1630 889 1629 Cuts at: 5978 8 Size: 43 Fragments arranged by size: 1630 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3460 1537 Size: 275 1648 4090 Fragments arranged by size: 4090 1648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2987 3943 1353 Size: 1634 956 3423 Fragments arranged by size: 3423 1634 956 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4600 4974 5120 387 Size: 1064 138 3011 374 146 1280 Fragments arranged by size: 3011 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2305 2505 2646 2697 2724 3273 4076 Size: 393 673 200 141 51 27 549 803 Cuts at: 4076 4673 5460 5728 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 803 787 673 597 549 393 268 200 141 51 27 EcoO109I rG'GnC_Cy Cuts at: 2578 2579 2578 Size: 1 6012 EcoRI G'AATT_C Cuts at: 3322 3700 3322 Size: 378 5635 FspI TGC'GCA Cuts at: 4519 4519 Size: 6013 HaeII r_GCGC'y Cuts at: 1678 5160 1678 Size: 3482 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2345 2377 2432 2462 2464 Size: 32 135 32 773 32 55 30 2 Cuts at: 2464 2494 2644 2676 2751 2783 4654 4686 4728 Size: 30 150 32 75 32 1871 32 42 Cuts at: 4728 4760 1373 Size: 32 2626 Fragments arranged by size: 2626 1871 773 150 135 75 55 42 32 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 3357 3603 669 Size: 988 281 1419 246 3079 Fragments arranged by size: 3079 1419 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6013 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2131 2579 5127 5311 1305 Size: 826 448 2548 184 2007 Fragments arranged by size: 2548 2007 826 448 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4191 Fragments arranged by size: 4191 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2845 3971 4330 4489 518 Size: 648 1679 1126 359 159 2042 Fragments arranged by size: 2042 1679 1126 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2492 3429 4106 5254 5499 1606 Size: 886 937 677 1148 245 2120 Fragments arranged by size: 2120 1148 937 886 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4504 Fragments arranged by size: 4504 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 2196 3371 4916 1132 Size: 97 967 1175 1545 2229 Fragments arranged by size: 2229 1545 1175 967 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6013 PciI A'CATG_T Cuts at: 4912 4912 Size: 6013 PflMI CCAn_nnn'nTGG Cuts at: 1108 2483 1108 Size: 1375 4638 PfoI T'CCnGG_A Cuts at: 2605 3250 2605 Size: 645 5368 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2521 2553 4082 4114 5595 5627 2521 Size: 32 1529 32 1481 32 2907 Fragments arranged by size: 2907 1529 1481 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PspOMI G'GGCC_C Cuts at: 2578 2578 Size: 6013 PstI C_TGCA'G Cuts at: 830 2536 3365 830 Size: 1706 829 3478 Fragments arranged by size: 3478 1706 829 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 PvuII CAG'CTG Cuts at: 2492 2492 Size: 6013 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 4261 5973 4261 Size: 1712 4301 SfcI C'TryA_G Cuts at: 826 1045 2064 2532 3138 3361 4496 5177 5368 Size: 219 1019 468 606 223 1135 681 191 Cuts at: 5368 826 Size: 1471 Fragments arranged by size: 1471 1135 1019 681 606 468 223 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 3340 1872 Size: 1468 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4117 5018 5280 820 Size: 197 926 2174 901 262 1553 Fragments arranged by size: 2174 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3310 3804 493 Size: 2817 494 2702 Fragments arranged by size: 2817 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SspI AAT'ATT Cuts at: 5 52 2807 3937 5 Size: 47 2755 1130 2081 Fragments arranged by size: 2755 2081 1130 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6013 StyI C'CwwG_G Cuts at: 513 1065 2022 3452 513 Size: 552 957 1430 3074 Fragments arranged by size: 3074 1430 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2015 4040 4057 4210 4395 4840 325 Size: 1255 435 2025 17 153 185 445 1498 Fragments arranged by size: 2025 1498 1255 445 435 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4259 5971 96 Size: 274 80 33 51 1443 2282 1712 138 Fragments arranged by size: 2282 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5973 6005 5973 Size: 32 5981 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6013 XbaI T'CTAG_A Cuts at: 2074 3349 2074 Size: 1275 4738 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2280 2280 Size: 6013 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6013 XmaI C'CCGG_G Cuts at: 1870 3338 1870 Size: 1468 4545 XmnI GAAnn'nnTTC Cuts at: 2548 4142 2548 Size: 1594 4419 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI