Restriction map for
pFN21ASDA1704
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BlnI BsaAI
BsaHI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CfoI
DraIII DrdI EaeI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HphI KasI KpnI MaeII
MfeI MluI MluNI MroI MscI
MslI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
RcaI SacI SalI SbfI ScaI
SfaNI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccII 49 CGCG CG^CG
AfaI 168 GTAC GT^AC
AlwI 394 GATCC GGATC(4/5)
ApaI 500 GGGCCC GGGCC^C
AvaI 78 CCCGAG C^[CT]CG[GA]G
BbvI 862 GCTGC GCAGC(8/12)
BcnI 34 CCCGG CC^[GC]GG
BfaI 971 CTAG C^TAG
BglII 915 AGATCT A^GATCT
BlpI 781 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 781 GCTGAGC GC^T[ACGT]AGC
BsaI 8 GAGACC GGTCTC(1/5)
BseRI 314 CTCCTC GAGGAG(10/8)
BsmFI 74 GGGAC GGGAC(10/14)
BsoBI 78 CCCGAG C^[CT]CG[GA]G
BspDI 656 ATCGAT AT^CGAT
BspMI 786 GCAGGT ACCTGC(4/8)
BsrBI 70 GAGCGG CCGCTC(-3/-3)
BsrDI 341 CATTGC GCAATG(2/0)
BsrFI 21 ACCGGC [GA]^CCGG[CT]
BstUI 49 CGCG CG^CG
BstYI 915 AGATCT [GA]^GATC[CT]
CelII 781 GCTGAGC GC^T[ACGT]AGC
Cfr10I 21 ACCGGC [GA]^CCGG[CT]
ClaI 656 ATCGAT AT^CGAT
CpoI 18 CGGACCG CG^G[AT]CCG
DraI 295 TTTAAA TTT^AAA
DsaI 48 CCGCGG C^C[GA][CT]GG
HincII 466 GTTAAC GT[CT]^[GA]AC
HindII 466 GTTAAC GT[CT]^[GA]AC
HindIII 880 AAGCTT A^AGCTT
HpaI 466 GTTAAC GTT^AAC
KspI 48 CCGCGG CCGC^GG
MaeI 971 CTAG C^TAG
MflI 915 AGATCT [GA]^GATC[CT]
MvnI 49 CGCG CG^CG
MwoI 91 GCAGGACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 34 CCCGG CC^[GC]GG
NlaIII 569 CATG CATG^
PleI 565 GAGTC GAGTC(4/5)
PvuII 680 CAGCTG CAG^CTG
RsaI 168 GTAC GT^AC
RsrII 18 CGGACCG CG^G[AT]CCG
SacII 48 CCGCGG CCGC^GG
SexAI 348 ACCTGGT A^CC[AT]GGT
SmlI 919 CTCAAG C^T[CT][GA]AG
TaqI 657 TCGA T^CGA
TseI 862 GCTGC G^C[AT]GC
Tsp45I 377 GTGAC ^GT[GC]AC
Tsp509I 112 AATT ^AATT
TthHB8I 657 TCGA T^CGA
XbaI 970 TCTAGA T^CTAGA
XhoII 915 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
BanII 81 GAGCCC G[GA]GC[CT]^C
500 GGGCCC
BbsI 151 GAAGAC GAAGAC(2/6)
868 GAAGAC
BmyI 81 GAGCCC G[AGT]GC[ACT]^C
500 GGGCCC
BpuAI 151 GAAGAC GAAGAC(2/6)
868 GAAGAC
BsaBI 815 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
910 GATAAAGATC
BsmAI 8 GAGAC GTCTC(1/5)
774 GAGAC
Bsp1286I 81 GAGCCC G[AGT]GC[ACT]^C
500 GGGCCC
Cac8I 46 GCCCGC GC[ACGT]^[ACGT]GC
450 GCCTGC
EcoNI 85 CCTGTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
451 CCTGCAAAAGG
EcoT14I 319 CCAAGG C^C[AT][AT]GG
616 CCAAGG
FauI 47 CCCGC CCCGC(4/6)
72 GCGGG
Fnu4HI 30 GCCGC GC^[ACGT]GC
862 GCTGC
HapII 22 CCGG C^CGG
35 CCGG
HpaII 22 CCGG C^CGG
35 CCGG
ItaI 30 GCCGC GC^[ACGT]GC
862 GCTGC
MaeIII 377 GTGAC ^GT[ACGT]AC
478 GTAAC
MamI 815 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
910 GATAAAGATC
MspI 22 CCGG C^CGG
35 CCGG
PpuMI 74 GGGACCC [GA]G^G[AT]CC[CT]
333 AGGTCCT
PshAI 202 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
601 GACTTTGGTC
SapI 67 GAAGAGC GCTCTTC(1/4)
632 GAAGAGC
StyI 319 CCAAGG C^C[AT][AT]GG
616 CCAAGG
TspRI 204 CAGTG CA[GC]TG[ACGT][ACGT]^
420 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AciI AluI AvaII BpmI BsaJI
BsiYI BslI BsrI BssKI BstF5I
BstNI Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII FokI HaeIII
HinfI Ksp632I MboI MboII MnlI
MseI MspA1I MvaI NdeII NlaIV
Sau3AI Sau96I ScrFI TfiI Tru9I