(Circular) (Six-base) MAPSORT of: pFN21ASDA1704.seq Check: 5620 from: 1 to: 5749 pFN21ASDA1704 5749 bp With 182 enzymes: SgfI * January 16, 2009 15:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3092 1937 Size: 1155 4594 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2235 2910 3812 5196 5464 1632 Size: 603 675 902 1384 268 1917 Fragments arranged by size: 1917 1384 902 675 603 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5749 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 3492 5064 3492 Size: 1572 4177 ApaI G_GGCC'C Cuts at: 2527 2527 Size: 5749 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 3058 3284 3436 5545 1493 Size: 1565 226 152 2109 1697 Fragments arranged by size: 2109 1697 1565 226 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 2101 3074 1870 Size: 73 158 973 4545 Fragments arranged by size: 4545 973 158 73 BamHI G'GATC_C Cuts at: 3079 3446 3079 Size: 367 5382 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3070 4525 618 Size: 325 217 627 1283 1455 1842 Fragments arranged by size: 1842 1455 1283 627 325 217 BanII G_rGCy'C Cuts at: 1391 2108 2527 3068 1391 Size: 717 419 541 4072 Fragments arranged by size: 4072 717 541 419 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2181 2898 928 Size: 971 282 717 3779 Fragments arranged by size: 3779 971 717 282 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 1548 Size: 5749 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4360 136 Size: 107 122 71 631 605 2688 1525 Fragments arranged by size: 2688 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2938 5744 2938 Size: 2806 2943 BlpI GC'TnA_GC Cuts at: 2805 3166 2805 Size: 361 5388 Bme1580I G_kGCm'C Cuts at: 1790 2527 3810 4966 1790 Size: 737 1283 1156 2573 Fragments arranged by size: 2573 1283 1156 737 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3480 4438 442 Size: 416 53 83 399 24 2063 958 1753 Fragments arranged by size: 2063 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2595 2685 2694 4409 1239 Size: 1356 90 9 1715 2579 Fragments arranged by size: 2579 1715 1356 90 9 Bpu10I CC'TnA_GC Cuts at: 2724 2724 Size: 5749 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2927 3874 4739 5037 2927 Size: 947 865 298 3639 Fragments arranged by size: 3639 947 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2025 4412 882 Size: 617 526 2387 2219 Fragments arranged by size: 2387 2219 617 526 BsaAI yAC'GTr Cuts at: 493 1204 3046 3540 493 Size: 711 1842 494 2702 Fragments arranged by size: 2702 1842 711 494 BsaBI GATnn'nnATC Cuts at: 2842 2937 5743 2842 Size: 95 2806 2848 Fragments arranged by size: 2848 2806 95 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3556 3938 4573 275 Size: 53 83 186 936 2023 382 635 1451 Fragments arranged by size: 2023 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2683 2713 1178 Size: 30 165 30 1280 30 4214 Fragments arranged by size: 4214 1280 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2328 1186 Size: 1142 4607 BseYI C'CCAG_C Cuts at: 4952 5448 4952 Size: 496 5253 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3199 3960 4988 1540 Size: 275 186 1198 761 1028 2301 Fragments arranged by size: 2301 1198 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3810 3895 4966 3810 Size: 85 1071 4593 Fragments arranged by size: 4593 1071 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2527 3068 3810 3895 4966 1391 Size: 399 318 419 541 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 541 419 399 318 85 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 3638 5368 3638 Size: 1730 4019 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2800 3104 844 Size: 1956 304 3489 Fragments arranged by size: 3489 1956 304 BsrBI CCG'CTC Cuts at: 1394 1823 2095 3636 1394 Size: 429 272 1541 3507 Fragments arranged by size: 3507 1541 429 272 BsrDI GCAATG_nn' Cuts at: 65 1248 2363 4244 4418 65 Size: 1183 1115 1881 174 1396 Fragments arranged by size: 1881 1396 1183 1115 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 4393 4580 1700 Size: 110 234 2349 187 2869 Fragments arranged by size: 2869 2349 234 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 2007 4553 2007 Size: 2546 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstYI r'GATC_y Cuts at: 1972 2938 3079 3446 3831 3848 5300 5386 5398 Size: 966 141 367 385 17 1452 86 12 Cuts at: 5398 5744 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 966 385 367 346 141 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2071 513 Size: 552 957 49 4191 Fragments arranged by size: 4191 957 552 49 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2184 487 Size: 683 56 958 4052 Fragments arranged by size: 4052 958 683 56 BtsI GCAGTG_nn' Cuts at: 2449 3265 4059 4079 2449 Size: 816 794 20 4119 Fragments arranged by size: 4119 816 794 20 ClaI AT'CG_AT Cuts at: 1554 2680 1554 Size: 1126 4623 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 2320 3053 2320 Size: 733 5016 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3196 4085 5714 Size: 54 1425 50 275 18 1366 889 1629 Cuts at: 5714 8 Size: 43 Fragments arranged by size: 1629 1425 1366 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2085 2650 2782 3679 1353 Size: 732 565 132 897 3423 Fragments arranged by size: 3423 897 732 565 132 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2235 2595 2685 2694 2910 3812 4409 Size: 393 603 360 90 9 216 902 597 Cuts at: 4409 5196 5464 1239 Size: 787 268 1524 Fragments arranged by size: 1524 902 787 603 597 393 360 268 216 90 9 EcoNI CCTnn'n_nnAGG Cuts at: 2112 2478 2112 Size: 366 5383 EcoO109I rG'GnC_Cy Cuts at: 2098 2357 2523 2098 Size: 259 166 5324 Fragments arranged by size: 5324 259 166 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 4896 1678 Size: 3218 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2022 2054 2242 2274 2552 Size: 32 135 32 450 32 188 32 278 Cuts at: 2552 2584 2654 2683 2686 2715 2798 2830 4390 Size: 32 70 29 3 29 83 32 1560 Cuts at: 4390 4422 4464 4496 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1560 450 278 188 135 83 70 42 32 32 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 2491 3093 3339 669 Size: 988 281 553 602 246 3079 Fragments arranged by size: 3079 988 602 553 281 246 HindIII A'AGCT_T Cuts at: 748 2903 748 Size: 2155 3594 HpaI GTT'AAC Cuts at: 2491 3339 2491 Size: 848 4901 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 3348 3348 Size: 5749 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2258 4863 5047 1305 Size: 953 2605 184 2007 Fragments arranged by size: 2605 2007 953 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3927 Fragments arranged by size: 3927 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3707 4066 4225 518 Size: 648 2541 359 159 2042 Fragments arranged by size: 2541 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2073 2081 2705 3165 3842 4990 5235 1606 Size: 467 8 624 460 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 624 467 460 245 8 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4240 Fragments arranged by size: 4240 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 3107 4652 1132 Size: 97 1878 1545 2229 Fragments arranged by size: 2229 1878 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5749 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5749 PfoI T'CCnGG_A Cuts at: 2311 2419 2311 Size: 108 5641 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2098 2357 2098 Size: 259 5490 PshAI GACnn'nnGTC Cuts at: 1613 2229 2628 1613 Size: 616 399 4734 Fragments arranged by size: 4734 616 399 PsiI TTA'TAA Cuts at: 2139 3319 2139 Size: 1180 4569 PspOMI G'GGCC_C Cuts at: 2523 2523 Size: 5749 PstI C_TGCA'G Cuts at: 830 3101 830 Size: 2271 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 PvuII CAG'CTG Cuts at: 2705 2705 Size: 5749 RsrII CG'GwC_CG Cuts at: 2042 2042 Size: 5749 SacII CC_GC'GG Cuts at: 2074 2074 Size: 5749 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SanDI GG'GwC_CC Cuts at: 2098 2098 Size: 5749 SapI GCTCTTCn'nnn_ Cuts at: 2085 2650 2085 Size: 565 5184 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SexAI A'CCwGG_T Cuts at: 2371 2371 Size: 5749 SfcI C'TryA_G Cuts at: 826 1045 3097 4232 4913 5104 826 Size: 219 2052 1135 681 191 1471 Fragments arranged by size: 2052 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 3076 1872 Size: 1204 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2942 3853 4754 5016 820 Size: 197 926 999 911 901 262 1553 Fragments arranged by size: 1553 999 926 911 901 262 197 SnaBI TAC'GTA Cuts at: 493 3046 3540 493 Size: 2553 494 2702 Fragments arranged by size: 2702 2553 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5749 StyI C'CwwG_G Cuts at: 513 1065 2022 2342 2639 3188 513 Size: 552 957 320 297 549 3074 Fragments arranged by size: 3074 957 552 549 320 297 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3776 3793 3946 4131 4576 325 Size: 1255 2196 17 153 185 445 1498 Fragments arranged by size: 2196 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3995 5707 96 Size: 274 80 33 51 1443 2018 1712 138 Fragments arranged by size: 2018 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5709 5741 5709 Size: 32 5717 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5749 XbaI T'CTAG_A Cuts at: 2993 3085 2993 Size: 92 5657 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 3074 1870 Size: 1204 4545 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI SacI SfiI SfoI SrfI SwaI XcmI