(Circular) (Six-base) MAPSORT of: pFN21ASDA1587.seq Check: 3167 from: 1 to: 7600 pFN21ASDA1587 7600 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2157 5410 278 Size: 53 83 186 936 621 3253 2468 Fragments arranged by size: 3253 2468 936 621 186 83 53 AccI GT'mk_AC Cuts at: 1937 4503 4943 1937 Size: 2566 440 4594 Fragments arranged by size: 4594 2566 440 Acc65I G'GTAC_C Cuts at: 2497 4183 4921 2497 Size: 1686 738 5176 Fragments arranged by size: 5176 1686 738 AclI AA'CG_TT Cuts at: 1563 5727 6100 1563 Size: 4164 373 3063 Fragments arranged by size: 4164 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3497 4049 4873 5663 7047 7315 1632 Size: 1865 552 824 790 1384 268 1917 Fragments arranged by size: 1917 1865 1384 824 790 552 268 AfeI AGC'GCT Cuts at: 4667 4667 Size: 7600 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7403 AflIII A'CryG_T Cuts at: 1203 6499 7476 1203 Size: 5296 977 1327 Fragments arranged by size: 5296 1327 977 AgeI A'CCGG_T Cuts at: 6431 6431 Size: 7600 AhdI GACnn_n'nnGTC Cuts at: 6329 6329 Size: 7600 AleI CACnn'nnGTG Cuts at: 1166 2258 2330 2402 2474 2546 2582 2618 2654 Size: 1092 72 72 72 72 36 36 36 Cuts at: 2654 2690 2726 2762 2798 2834 2906 2942 2978 Size: 36 36 36 36 36 72 36 36 Cuts at: 2978 3014 3050 3122 3158 3194 1166 Size: 36 36 72 36 36 5572 Fragments arranged by size: 5572 1092 72 72 72 72 72 72 36 36 36 36 36 36 36 36 36 36 36 36 36 36 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2276 2348 2492 2528 2564 2636 2672 2708 Size: 1092 72 144 36 36 72 36 36 Cuts at: 2708 2744 2780 2816 2852 2924 2960 2970 2996 Size: 36 36 36 36 72 36 10 26 Cuts at: 2996 3032 3068 3140 3212 1184 Size: 36 36 72 72 5572 Fragments arranged by size: 5572 1092 144 72 72 72 72 72 36 36 36 36 36 36 36 36 36 36 36 26 10 AlwNI CAG_nnn'CTG Cuts at: 2094 3362 3526 3953 5343 6915 2094 Size: 1268 164 427 1390 1572 2779 Fragments arranged by size: 2779 1572 1390 1268 427 164 ApaI G_GGCC'C Cuts at: 2213 2249 3257 3992 2213 Size: 36 1008 735 5821 Fragments arranged by size: 5821 1008 735 36 ApaLI G'TGCA_C Cuts at: 2395 5657 6813 2395 Size: 3262 1156 3182 Fragments arranged by size: 3262 3182 1156 ApoI r'AATT_y Cuts at: 1493 2041 3395 4023 4332 4909 5135 5287 7396 Size: 548 1354 628 309 577 226 152 2109 Cuts at: 7396 1493 Size: 1697 Fragments arranged by size: 2109 1697 1354 628 577 548 309 226 152 AseI AT'TA_AT Cuts at: 160 1032 6154 160 Size: 872 5122 1606 Fragments arranged by size: 5122 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7600 AvaI C'yCGr_G Cuts at: 1870 1943 3256 3459 4925 1870 Size: 73 1313 203 1466 4545 Fragments arranged by size: 4545 1466 1313 203 73 AvrII C'CTAG_G Cuts at: 3329 3329 Size: 7600 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4645 4678 4645 Size: 33 7567 BamHI G'GATC_C Cuts at: 4930 5297 4930 Size: 367 7233 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2324 2497 2587 2648 2875 Size: 325 217 627 537 173 90 61 227 Cuts at: 2875 4183 4921 6376 618 Size: 1308 738 1455 1842 Fragments arranged by size: 1842 1455 1308 738 627 537 325 227 217 173 90 61 BanII G_rGCy'C Cuts at: 1391 2213 2249 3257 3425 3749 3992 4738 4919 Size: 822 36 1008 168 324 243 746 181 Cuts at: 4919 1391 Size: 4072 Fragments arranged by size: 4072 1008 822 746 324 243 181 168 36 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6629 BbvCI CC'TCA_GC Cuts at: 2241 4122 2241 Size: 1881 5719 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5791 5825 1903 Size: 34 3854 34 3678 Fragments arranged by size: 3854 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5492 6702 5492 Size: 1210 6390 BclI T'GATC_A Cuts at: 1548 1548 Size: 7600 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3821 6211 136 Size: 107 122 71 631 605 2149 2390 1525 Fragments arranged by size: 2390 2149 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7595 7595 Size: 7600 BlpI GC'TnA_GC Cuts at: 3510 5017 3510 Size: 1507 6093 Bme1580I G_kGCm'C Cuts at: 1790 2213 2249 2399 2592 2880 2913 3257 3726 Size: 423 36 150 193 288 33 344 469 Cuts at: 3726 3992 4866 5661 6817 1790 Size: 266 874 795 1156 2573 Fragments arranged by size: 2573 1156 874 795 469 423 344 288 266 193 150 36 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7600 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2340 3722 5331 Size: 416 53 83 399 24 923 1382 1609 Cuts at: 5331 6289 442 Size: 958 1753 Fragments arranged by size: 1753 1609 1382 958 923 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7600 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2422 3662 3865 6260 1239 Size: 932 251 1240 203 2395 2579 Fragments arranged by size: 2579 2395 1240 932 251 203 Bpu10I CC'TnA_GC Cuts at: 2241 2357 2681 2753 2933 4122 4547 4612 2241 Size: 116 324 72 180 1189 425 65 5229 Fragments arranged by size: 5229 1189 425 324 180 116 72 65 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2269 4678 5725 6590 6888 2269 Size: 2409 1047 865 298 2981 Fragments arranged by size: 2981 2409 1047 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2132 6263 882 Size: 617 633 4131 2219 Fragments arranged by size: 4131 2219 633 617 BsaAI yAC'GTr Cuts at: 493 1204 4897 5391 493 Size: 711 3693 494 2702 Fragments arranged by size: 3693 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7594 7594 Size: 7600 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2154 5407 5789 6424 Size: 53 83 186 936 621 3253 382 635 Cuts at: 6424 275 Size: 1451 Fragments arranged by size: 3253 1451 936 635 621 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3291 6033 6431 6705 6852 7263 3291 Size: 2742 398 274 147 411 3628 Fragments arranged by size: 3628 2742 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3962 3992 1178 Size: 30 165 30 2559 30 4786 Fragments arranged by size: 4786 2559 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3050 4508 1186 Size: 1864 1458 4278 Fragments arranged by size: 4278 1864 1458 BseYI C'CCAG_C Cuts at: 2094 2168 4625 6803 7299 2094 Size: 74 2457 2178 496 2395 Fragments arranged by size: 2457 2395 2178 496 74 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2714 2966 3218 3399 3878 4301 4378 Size: 573 978 252 252 181 479 423 77 Cuts at: 4378 1163 Size: 4385 Fragments arranged by size: 4385 978 573 479 423 252 252 181 77 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5050 5811 6839 1540 Size: 275 186 3049 761 1028 2301 Fragments arranged by size: 3049 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2399 2507 2543 2687 2723 2795 2831 2939 2975 Size: 108 36 144 36 72 36 108 36 Cuts at: 2975 3011 3047 3119 3263 5661 5746 6817 2399 Size: 36 36 72 144 2398 85 1071 3182 Fragments arranged by size: 3182 2398 1071 144 144 108 108 85 72 72 36 36 36 36 36 36 BsmI GAATG_Cn' Cuts at: 5111 5204 5111 Size: 93 7507 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2213 2249 2399 2507 2543 2592 2687 Size: 399 423 36 150 108 36 49 95 Cuts at: 2687 2723 2795 2831 2880 2913 2939 2975 3011 Size: 36 72 36 49 33 26 36 36 Cuts at: 3011 3047 3119 3257 3263 3425 3726 3749 3992 Size: 36 72 138 6 162 301 23 243 Cuts at: 3992 4738 4866 4919 5661 5746 6817 1391 Size: 746 128 53 742 85 1071 2174 Fragments arranged by size: 2174 1071 746 742 423 399 301 243 162 150 138 128 108 95 85 72 72 53 49 49 36 36 36 36 36 36 36 33 26 23 6 BspEI T'CCGG_A Cuts at: 7263 7263 Size: 7600 BspHI T'CATG_A Cuts at: 5489 7219 5489 Size: 1730 5870 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4531 4655 4955 844 Size: 3687 124 300 3489 Fragments arranged by size: 3687 3489 300 124 BsrBI CCG'CTC Cuts at: 1394 1823 2662 5487 1394 Size: 429 839 2825 3507 Fragments arranged by size: 3507 2825 839 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4836 6095 6269 65 Size: 1183 3588 1259 174 1396 Fragments arranged by size: 3588 1396 1259 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2803 6244 6431 1700 Size: 110 993 3441 187 2869 Fragments arranged by size: 3441 2869 993 187 110 BsrGI T'GTAC_A Cuts at: 96 4464 96 Size: 4368 3232 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7600 BssSI C'ACGA_G Cuts at: 5660 6672 5660 Size: 1012 6588 BstAPI GCAn_nnn'nTGC Cuts at: 794 3526 794 Size: 2732 4868 BstBI TT'CG_AA Cuts at: 2007 6404 2007 Size: 4397 3203 BstEII G'GTnAC_C Cuts at: 1184 3334 1184 Size: 2150 5450 BstXI CCAn_nnnn'nTGG Cuts at: 2029 4239 4415 4806 2029 Size: 2210 176 391 4823 Fragments arranged by size: 4823 2210 391 176 BstYI r'GATC_y Cuts at: 1972 2181 3995 4057 4930 5297 5682 5699 7151 Size: 209 1814 62 873 367 385 17 1452 Cuts at: 7151 7237 7249 7595 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1814 1452 873 385 367 346 209 86 62 17 12 Bsu36I CC'TnA_GG Cuts at: 3372 3681 3372 Size: 309 7291 BtgI C'CryG_G Cuts at: 513 1065 2583 2884 3348 513 Size: 552 1518 301 464 4765 Fragments arranged by size: 4765 1518 552 464 301 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6861 Fragments arranged by size: 6861 683 56 BtsI GCAGTG_nn' Cuts at: 2952 5116 5910 5930 2952 Size: 2164 794 20 4622 Fragments arranged by size: 4622 2164 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7600 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7565 DraI TTT'AAA Cuts at: 4904 4904 Size: 7600 DraIII CAC_nnn'GTG Cuts at: 2636 2636 Size: 7600 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6607 809 Size: 507 5291 1802 Fragments arranged by size: 5291 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2805 4070 4348 Size: 54 1425 50 275 18 975 1265 278 Cuts at: 4348 4776 4808 5047 5936 7565 8 Size: 428 32 239 889 1629 43 Fragments arranged by size: 1629 1425 1265 975 889 428 278 275 239 54 50 43 32 18 EagI C'GGCC_G Cuts at: 1537 1812 5047 1537 Size: 275 3235 4090 Fragments arranged by size: 4090 3235 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3735 5530 1353 Size: 2382 1795 3423 Fragments arranged by size: 3423 2382 1795 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6187 6561 6707 387 Size: 1064 138 4598 374 146 1280 Fragments arranged by size: 4598 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2422 3497 3662 3865 4049 4873 Size: 393 539 251 1075 165 203 184 824 Cuts at: 4873 5663 6260 7047 7315 1239 Size: 790 597 787 268 1524 Fragments arranged by size: 1524 1075 824 790 787 597 539 393 268 251 203 184 165 EcoO109I rG'GnC_Cy Cuts at: 2110 2165 2191 2209 2245 2318 2353 2677 2749 Size: 55 26 18 36 73 35 324 72 Cuts at: 2749 2929 3253 3368 3386 3403 3716 3988 4155 Size: 180 324 115 18 17 313 272 167 Cuts at: 4155 4543 4727 2110 Size: 388 184 4983 Fragments arranged by size: 4983 388 324 324 313 272 184 180 167 115 73 72 55 36 35 26 18 18 17 EcoRI G'AATT_C Cuts at: 2041 3395 4332 4909 5287 2041 Size: 1354 937 577 378 4354 Fragments arranged by size: 4354 1354 937 577 378 FspI TGC'GCA Cuts at: 2082 6106 2082 Size: 4024 3576 HaeII r_GCGC'y Cuts at: 1678 2292 4669 6747 1678 Size: 614 2377 2078 2531 Fragments arranged by size: 2531 2377 2078 614 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2850 2882 4172 4204 6241 Size: 32 135 32 1278 32 1290 32 2037 Cuts at: 6241 6273 6315 6347 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2037 1290 1278 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4944 5190 669 Size: 988 281 3006 246 3079 Fragments arranged by size: 3079 3006 988 281 246 HindIII A'AGCT_T Cuts at: 748 4549 748 Size: 3801 3799 HpaI GTT'AAC Cuts at: 5190 5190 Size: 7600 KpnI G_GTAC'C Cuts at: 2501 4187 4925 2501 Size: 1686 738 5176 Fragments arranged by size: 5176 1686 738 MfeI C'AATT_G Cuts at: 5199 5199 Size: 7600 MluI A'CGCG_T Cuts at: 7476 7476 Size: 7600 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6714 6898 1305 Size: 5409 184 2007 Fragments arranged by size: 5409 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4072 4778 4810 10 Size: 54 1425 343 2240 706 32 2800 Fragments arranged by size: 2800 2240 1425 706 343 54 32 MslI CAynn'nnrTG Cuts at: 518 1166 2258 2330 2402 2474 2546 2582 2618 Size: 648 1092 72 72 72 72 36 36 Cuts at: 2618 2654 2690 2726 2762 2798 2834 2906 2942 Size: 36 36 36 36 36 36 72 36 Cuts at: 2942 2978 3014 3050 3122 3158 3194 4016 4237 Size: 36 36 36 72 36 36 822 221 Cuts at: 4237 4413 5558 5917 6076 518 Size: 176 1145 359 159 2042 Fragments arranged by size: 2042 1145 1092 822 648 359 221 176 159 72 72 72 72 72 72 36 36 36 36 36 36 36 36 36 36 36 36 36 36 MspA1I CmG'CkG Cuts at: 1606 2094 2135 2240 2886 3862 5016 5693 6841 Size: 488 41 105 646 976 1154 677 1148 Cuts at: 6841 7086 1606 Size: 245 2120 Fragments arranged by size: 2120 1154 1148 976 677 646 488 245 105 41 NaeI GCC'GGC Cuts at: 2805 2805 Size: 7600 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7048 NdeI CA'TA_TG Cuts at: 387 387 Size: 7600 NgoMIV G'CCGG_C Cuts at: 2803 2803 Size: 7600 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7600 NotI GC'GGCC_GC Cuts at: 5047 5047 Size: 7600 NspI r_CATG'y Cuts at: 1132 1229 4640 4958 6503 1132 Size: 97 3411 318 1545 2229 Fragments arranged by size: 3411 2229 1545 318 97 PasI CC'CwG_GG Cuts at: 1348 2161 1348 Size: 813 6787 PciI A'CATG_T Cuts at: 6499 6499 Size: 7600 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7600 PfoI T'CCnGG_A Cuts at: 3558 3558 Size: 7600 PmeI GTTT'AAAC Cuts at: 4904 4904 Size: 7600 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2011 2043 5669 5701 7182 7214 2011 Size: 32 3626 32 1481 32 2397 Fragments arranged by size: 3626 2397 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2165 2318 3368 3403 3716 4543 4727 2165 Size: 153 1050 35 313 827 184 5038 Fragments arranged by size: 5038 1050 827 313 184 153 35 PshAI GACnn'nnGTC Cuts at: 1613 3904 1613 Size: 2291 5309 PsiI TTA'TAA Cuts at: 5170 5170 Size: 7600 PspOMI G'GGCC_C Cuts at: 2209 2245 3253 3988 2209 Size: 36 1008 735 5821 Fragments arranged by size: 5821 1008 735 36 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 4416 4448 4416 Size: 32 7568 PstI C_TGCA'G Cuts at: 830 4180 4591 4652 4952 830 Size: 3350 411 61 300 3478 Fragments arranged by size: 3478 3350 411 300 61 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7600 PvuII CAG'CTG Cuts at: 2094 2094 Size: 7600 RsrII CG'GwC_CG Cuts at: 3218 3218 Size: 7600 SacII CC_GC'GG Cuts at: 2887 2887 Size: 7600 SalI G'TCGA_C Cuts at: 1936 4942 1936 Size: 3006 4594 SanDI GG'GwC_CC Cuts at: 2165 2165 Size: 7600 SbfI CC_TGCA'GG Cuts at: 4952 4952 Size: 7600 ScaI AGT'ACT Cuts at: 5848 7560 5848 Size: 1712 5888 SexAI A'CCwGG_T Cuts at: 2632 2632 Size: 7600 SfcI C'TryA_G Cuts at: 826 1045 3390 3940 4176 4587 4648 4948 6083 Size: 219 2345 550 236 411 61 300 1135 Cuts at: 6083 6764 6955 826 Size: 681 191 1471 Fragments arranged by size: 2345 1471 1135 681 550 411 300 236 219 191 61 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7600 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7600 SmaI CCC'GGG Cuts at: 1872 3461 4927 1872 Size: 1589 1466 4545 Fragments arranged by size: 4545 1589 1466 SmlI C'TyrA_G Cuts at: 820 1017 1943 2284 4657 5704 6605 6867 820 Size: 197 926 341 2373 1047 901 262 1553 Fragments arranged by size: 2373 1553 1047 926 901 341 262 197 SnaBI TAC'GTA Cuts at: 493 4897 5391 493 Size: 4404 494 2702 Fragments arranged by size: 4404 2702 494 SpeI A'CTAG_T Cuts at: 152 4426 152 Size: 4274 3326 SphI G_CATG'C Cuts at: 1132 4958 1132 Size: 3826 3774 SspI AAT'ATT Cuts at: 5 52 3795 5524 5 Size: 47 3743 1729 2081 Fragments arranged by size: 3743 2081 1729 47 StuI AGG'CCT Cuts at: 1517 2282 3344 4519 1517 Size: 765 1062 1175 4598 Fragments arranged by size: 4598 1175 1062 765 StyI C'CwwG_G Cuts at: 513 1065 2871 3329 3339 3816 5039 513 Size: 552 1806 458 10 477 1223 3074 Fragments arranged by size: 3074 1806 1223 552 477 458 10 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2731 2785 2803 2839 5627 5644 5797 Size: 1255 1151 54 18 36 2788 17 153 Cuts at: 5797 5982 6427 325 Size: 185 445 1498 Fragments arranged by size: 2788 1498 1255 1151 445 185 153 54 36 18 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4338 4464 4818 Size: 274 80 33 51 1443 2361 126 354 Cuts at: 4818 5846 7558 96 Size: 1028 1712 138 Fragments arranged by size: 2361 1712 1443 1028 354 274 138 126 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3216 3248 7560 7592 3216 Size: 32 4312 32 3224 Fragments arranged by size: 4312 3224 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7600 XbaI T'CTAG_A Cuts at: 4936 4936 Size: 7600 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3346 3439 3346 Size: 93 7507 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7600 XmaI C'CCGG_G Cuts at: 1870 3459 4925 1870 Size: 1589 1466 4545 Fragments arranged by size: 4545 1589 1466 XmnI GAAnn'nnTTC Cuts at: 5729 5729 Size: 7600 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2155 5408 276 Size: 53 83 186 936 621 3253 2468 Fragments arranged by size: 3253 2468 936 621 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI SacI SapI SfiI SfoI SrfI SwaI