Restriction map for
pFN21ASDA1587
(Linear) (Six-base) MAPSORT of: KIAA1587.seq Check: 9323 from: 1 to: 2871
KIAA1587.orf
With 182 enzymes: SgfI *
October 24, 2006 15:17 ..
AatII G_ACGT'C
Cuts at: 0 134 2871
Size: 134 2737
AccI GT'mk_AC
Cuts at: 0 2480 2871
Size: 2480 391
Acc65I G'GTAC_C
Cuts at: 0 474 2160 2871
Size: 474 1686 711
Fragments arranged by size:
1686 711 474
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1474 2026 2850 2871
Size: 1474 552 824 21
Fragments arranged by size:
1474 824 552 21
AfeI AGC'GCT
Cuts at: 0 2644 2871
Size: 2644 227
AleI CACnn'nnGTG
Cuts at: 0 235 307 379 451 523 559 595 631
Size: 235 72 72 72 72 36 36 36
Cuts at: 631 667 703 739 775 811 883 919 955
Size: 36 36 36 36 36 72 36 36
Cuts at: 955 991 1027 1099 1135 1171 2871
Size: 36 36 72 36 36 1700
Fragments arranged by size:
1700 235 72 72 72 72 72 72
36 36 36 36 36 36 36 36
36 36 36 36 36 36
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 253 325 469 505 541 613 649 685
Size: 253 72 144 36 36 72 36 36
Cuts at: 685 721 757 793 829 901 937 947 973
Size: 36 36 36 36 72 36 10 26
Cuts at: 973 1009 1045 1117 1189 2871
Size: 36 36 72 72 1682
Fragments arranged by size:
1682 253 144 72 72 72 72 72
36 36 36 36 36 36 36 36
36 36 36 26 10
AlwNI CAG_nnn'CTG
Cuts at: 0 71 1339 1503 1930 2871
Size: 71 1268 164 427 941
Fragments arranged by size:
1268 941 427 164 71
ApaI G_GGCC'C
Cuts at: 0 190 226 1234 1969 2871
Size: 190 36 1008 735 902
Fragments arranged by size:
1008 902 735 190 36
ApaLI G'TGCA_C
Cuts at: 0 372 2871
Size: 372 2499
ApoI r'AATT_y
Cuts at: 0 18 1372 2000 2309 2871
Size: 18 1354 628 309 562
Fragments arranged by size:
1354 628 562 309 18
AvaI C'yCGr_G
Cuts at: 0 1233 1436 2871
Size: 1233 203 1435
Fragments arranged by size:
1435 1233 203
AvrII C'CTAG_G
Cuts at: 0 1306 2871
Size: 1306 1565
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2622 2655 2871
Size: 2622 33 216
Fragments arranged by size:
2622 216 33
BanI G'GyrC_C
Cuts at: 0 301 474 564 625 852 2160 2871
Size: 301 173 90 61 227 1308 711
Fragments arranged by size:
1308 711 301 227 173 90 61
BanII G_rGCy'C
Cuts at: 0 190 226 1234 1402 1726 1969 2715 2871
Size: 190 36 1008 168 324 243 746 156
Fragments arranged by size:
1008 746 324 243 190 168 156 36
BbvCI CC'TCA_GC
Cuts at: 0 218 2099 2871
Size: 218 1881 772
Fragments arranged by size:
1881 772 218
BglI GCCn_nnn'nGGC
Cuts at: 0 1798 2871
Size: 1798 1073
BlpI GC'TnA_GC
Cuts at: 0 1487 2871
Size: 1487 1384
Bme1580I G_kGCm'C
Cuts at: 0 190 226 376 569 857 890 1234 1703
Size: 190 36 150 193 288 33 344 469
Cuts at: 1703 1969 2843 2871
Size: 266 874 28
Fragments arranged by size:
874 469 344 288 266 193 190 150
36 33 28
BmrI ACTGGGnnnn_n'
Cuts at: 0 317 1699 2871
Size: 317 1382 1172
Fragments arranged by size:
1382 1172 317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 399 1639 1842 2871
Size: 148 251 1240 203 1029
Fragments arranged by size:
1240 1029 251 203 148
Bpu10I CC'TnA_GC
Cuts at: 0 218 334 658 730 910 2099 2524 2589
Size: 218 116 324 72 180 1189 425 65
Cuts at: 2589 2871
Size: 282
Fragments arranged by size:
1189 425 324 282 218 180 116 72
65
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 246 2655 2871
Size: 246 2409 216
Fragments arranged by size:
2409 246 216
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 2871
Size: 109 2762
BsaHI Gr'CG_yC
Cuts at: 0 131 2871
Size: 131 2740
BsaWI w'CCGG_w
Cuts at: 0 1268 2871
Size: 1268 1603
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1939 1969 2871
Size: 1939 30 902
Fragments arranged by size:
1939 902 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1027 2485 2871
Size: 1027 1458 386
Fragments arranged by size:
1458 1027 386
BseYI C'CCAG_C
Cuts at: 0 71 145 2602 2871
Size: 71 74 2457 269
Fragments arranged by size:
2457 269 74 71
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 691 943 1195 1376 1855 2278 2355 2871
Size: 691 252 252 181 479 423 77 516
Fragments arranged by size:
691 516 479 423 252 252 181 77
BsiHKAI G_wGCw'C
Cuts at: 0 376 484 520 664 700 772 808 916
Size: 376 108 36 144 36 72 36 108
Cuts at: 916 952 988 1024 1096 1240 2871
Size: 36 36 36 72 144 1631
Fragments arranged by size:
1631 376 144 144 108 108 72 72
36 36 36 36 36 36
Bsp1286I G_dGCh'C
Cuts at: 0 190 226 376 484 520 569 664 700
Size: 190 36 150 108 36 49 95 36
Cuts at: 700 772 808 857 890 916 952 988 1024
Size: 72 36 49 33 26 36 36 36
Cuts at: 1024 1096 1234 1240 1402 1703 1726 1969 2715
Size: 72 138 6 162 301 23 243 746
Cuts at: 2715 2843 2871
Size: 128 28
Fragments arranged by size:
746 301 243 190 162 150 138 128
108 95 72 72 49 49 36 36
36 36 36 36 36 33 28 26
23 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2508 2632 2871
Size: 2508 124 239
Fragments arranged by size:
2508 239 124
BsrBI CCG'CTC
Cuts at: 0 639 2871
Size: 639 2232
BsrDI GCAATG_nn'
Cuts at: 0 2813 2871
Size: 2813 58
BsrFI r'CCGG_y
Cuts at: 0 780 2871
Size: 780 2091
BsrGI T'GTAC_A
Cuts at: 0 2441 2871
Size: 2441 430
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1503 2871
Size: 1503 1368
BstEII G'GTnAC_C
Cuts at: 0 1311 2871
Size: 1311 1560
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2216 2392 2783 2871
Size: 2216 176 391 88
Fragments arranged by size:
2216 391 176 88
BstYI r'GATC_y
Cuts at: 0 158 1972 2034 2871
Size: 158 1814 62 837
Fragments arranged by size:
1814 837 158 62
Bsu36I CC'TnA_GG
Cuts at: 0 1349 1658 2871
Size: 1349 309 1213
Fragments arranged by size:
1349 1213 309
BtgI C'CryG_G
Cuts at: 0 560 861 1325 2871
Size: 560 301 464 1546
Fragments arranged by size:
1546 560 464 301
BtsI GCAGTG_nn'
Cuts at: 0 929 2871
Size: 929 1942
DraIII CAC_nnn'GTG
Cuts at: 0 613 2871
Size: 613 2258
EaeI y'GGCC_r
Cuts at: 0 782 2047 2325 2753 2785 2871
Size: 782 1265 278 428 32 86
Fragments arranged by size:
1265 782 428 278 86 32
EarI CTCTTCn'nnn_
Cuts at: 0 1712 2871
Size: 1712 1159
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 399 1474 1639 1842 2026 2850 2871
Size: 148 251 1075 165 203 184 824 21
Fragments arranged by size:
1075 824 251 203 184 165 148 21
EcoO109I rG'GnC_Cy
Cuts at: 0 87 142 168 186 222 295 330 654
Size: 87 55 26 18 36 73 35 324
Cuts at: 654 726 906 1230 1345 1363 1380 1693 1965
Size: 72 180 324 115 18 17 313 272
Cuts at: 1965 2132 2520 2704 2871
Size: 167 388 184 167
Fragments arranged by size:
388 324 324 313 272 184 180 167
167 115 87 73 72 55 36 35
26 18 18 17
EcoRI G'AATT_C
Cuts at: 0 18 1372 2309 2871
Size: 18 1354 937 562
Fragments arranged by size:
1354 937 562 18
FspI TGC'GCA
Cuts at: 0 59 2871
Size: 59 2812
HaeII r_GCGC'y
Cuts at: 0 269 2646 2871
Size: 269 2377 225
Fragments arranged by size:
2377 269 225
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 827 859 2149 2181 2871
Size: 827 32 1290 32 690
Fragments arranged by size:
1290 827 690 32 32
HindIII A'AGCT_T
Cuts at: 0 2526 2871
Size: 2526 345
KpnI G_GTAC'C
Cuts at: 0 478 2164 2871
Size: 478 1686 707
Fragments arranged by size:
1686 707 478
MscI TGG'CCA
Cuts at: 0 2049 2755 2787 2871
Size: 2049 706 32 84
Fragments arranged by size:
2049 706 84 32
MslI CAynn'nnrTG
Cuts at: 0 235 307 379 451 523 559 595 631
Size: 235 72 72 72 72 36 36 36
Cuts at: 631 667 703 739 775 811 883 919 955
Size: 36 36 36 36 36 72 36 36
Cuts at: 955 991 1027 1099 1135 1171 1993 2214 2390
Size: 36 36 72 36 36 822 221 176
Cuts at: 2390 2871
Size: 481
Fragments arranged by size:
822 481 235 221 176 72 72 72
72 72 72 36 36 36 36 36
36 36 36 36 36 36 36 36
36
MspA1I CmG'CkG
Cuts at: 0 71 112 217 863 1839 2871
Size: 71 41 105 646 976 1032
Fragments arranged by size:
1032 976 646 105 71 41
NaeI GCC'GGC
Cuts at: 0 782 2871
Size: 782 2089
NgoMIV G'CCGG_C
Cuts at: 0 780 2871
Size: 780 2091
NspI r_CATG'y
Cuts at: 0 2617 2871
Size: 2617 254
PasI CC'CwG_GG
Cuts at: 0 138 2871
Size: 138 2733
PfoI T'CCnGG_A
Cuts at: 0 1535 2871
Size: 1535 1336
PpuMI rG'GwC_Cy
Cuts at: 0 142 295 1345 1380 1693 2520 2704 2871
Size: 142 153 1050 35 313 827 184 167
Fragments arranged by size:
1050 827 313 184 167 153 142 35
PshAI GACnn'nnGTC
Cuts at: 0 1881 2871
Size: 1881 990
PspOMI G'GGCC_C
Cuts at: 0 186 222 1230 1965 2871
Size: 186 36 1008 735 906
Fragments arranged by size:
1008 906 735 186 36
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2393 2425 2871
Size: 2393 32 446
Fragments arranged by size:
2393 446 32
PstI C_TGCA'G
Cuts at: 0 2157 2568 2629 2871
Size: 2157 411 61 242
Fragments arranged by size:
2157 411 242 61
PvuII CAG'CTG
Cuts at: 0 71 2871
Size: 71 2800
RsrII CG'GwC_CG
Cuts at: 0 1195 2871
Size: 1195 1676
SacII CC_GC'GG
Cuts at: 0 864 2871
Size: 864 2007
SanDI GG'GwC_CC
Cuts at: 0 142 2871
Size: 142 2729
SexAI A'CCwGG_T
Cuts at: 0 609 2871
Size: 609 2262
SfcI C'TryA_G
Cuts at: 0 1367 1917 2153 2564 2625 2871
Size: 1367 550 236 411 61 246
Fragments arranged by size:
1367 550 411 246 236 61
SmaI CCC'GGG
Cuts at: 0 1438 2871
Size: 1438 1433
SmlI C'TyrA_G
Cuts at: 0 261 2634 2871
Size: 261 2373 237
Fragments arranged by size:
2373 261 237
SpeI A'CTAG_T
Cuts at: 0 2403 2871
Size: 2403 468
SspI AAT'ATT
Cuts at: 0 1772 2871
Size: 1772 1099
StuI AGG'CCT
Cuts at: 0 259 1321 2496 2871
Size: 259 1062 1175 375
Fragments arranged by size:
1175 1062 375 259
StyI C'CwwG_G
Cuts at: 0 848 1306 1316 1793 2871
Size: 848 458 10 477 1078
Fragments arranged by size:
1078 848 477 458 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 708 762 780 816 2871
Size: 708 54 18 36 2055
Fragments arranged by size:
2055 708 54 36 18
TatI w'GTAC_w
Cuts at: 0 2315 2441 2795 2871
Size: 2315 126 354 76
Fragments arranged by size:
2315 354 126 76
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1193 1225 2871
Size: 1193 32 1646
Fragments arranged by size:
1646 1193 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1323 1416 2871
Size: 1323 93 1455
Fragments arranged by size:
1455 1323 93
XmaI C'CCGG_G
Cuts at: 0 1436 2871
Size: 1436 1435
ZraI GAC'GTC
Cuts at: 0 132 2871
Size: 132 2739
Enzymes that do cut:
AatII AccI Acc65I AcuI AfeI AleI AlfI AlwNI
ApaI ApaLI ApoI AvaI AvrII BaeI BanI BanII
BbvCI BglI BlpI Bme1580I BmrI BpmI Bpu10I BpuEI
BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HindIII KpnI
MscI MslI MspA1I NaeI NgoMIV NspI PasI PfoI
PpuMI PshAI PspOMI PsrI PstI PvuII RsrII SacII
SanDI SexAI SfcI SmaI SmlI SpeI SspI StuI
StyI TaqII TatI TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI AclI AflII AflIII AgeI AhdI AloI AscI
AseI AsiSI BamHI BbeI BbsI BcgI BciVI BclI
BfrBI BglII BmgBI BmtI BplI BsaAI BsaBI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BssHII BssSI BstBI
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoICRI EcoNI EcoRV FalI FseI FspAI HincII HpaI
KasI MfeI MluI MmeI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PsiI PspXI PvuI SacI SalI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SnaBI SphI SrfI
SwaI Tth111I XbaI XhoI XmnI