(Circular) (Six-base) MAPSORT of: pFN21ASDA1559.seq Check: 6587 from: 1 to: 6328 pFN21ASDA1559 6328 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4138 278 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3671 1937 Size: 1734 4594 Acc65I G'GTAC_C Cuts at: 3649 3649 Size: 6328 AclI AA'CG_TT Cuts at: 1563 4455 4828 1563 Size: 2892 373 3063 Fragments arranged by size: 3063 2892 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2348 4391 5775 6043 1632 Size: 716 2043 1384 268 1917 Fragments arranged by size: 2043 1917 1384 716 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6131 AflIII A'CryG_T Cuts at: 1203 5227 6204 1203 Size: 4024 977 1327 Fragments arranged by size: 4024 1327 977 AgeI A'CCGG_T Cuts at: 2690 5159 2690 Size: 2469 3859 AhdI GACnn_n'nnGTC Cuts at: 5057 5057 Size: 6328 AleI CACnn'nnGTG Cuts at: 1166 2691 2859 3363 3447 1166 Size: 1525 168 504 84 4047 Fragments arranged by size: 4047 1525 504 168 84 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6328 AlwNI CAG_nnn'CTG Cuts at: 2833 4071 5643 2833 Size: 1238 1572 3518 Fragments arranged by size: 3518 1572 1238 ApaLI G'TGCA_C Cuts at: 2658 4385 5541 2658 Size: 1727 1156 3445 Fragments arranged by size: 3445 1727 1156 ApoI r'AATT_y Cuts at: 1493 2084 2598 2850 3018 3522 3618 3637 3863 Size: 591 514 252 168 504 96 19 226 Cuts at: 3863 4015 6124 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 591 514 504 252 226 168 152 96 19 AseI AT'TA_AT Cuts at: 160 1032 4882 160 Size: 872 3850 1606 Fragments arranged by size: 3850 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 AvaI C'yCGr_G Cuts at: 1870 1943 3653 1870 Size: 73 1710 4545 Fragments arranged by size: 4545 1710 73 BamHI G'GATC_C Cuts at: 2092 3658 4025 2092 Size: 1566 367 4395 Fragments arranged by size: 4395 1566 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3649 5104 618 Size: 325 217 627 1862 1455 1842 Fragments arranged by size: 1862 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3647 1391 Size: 2256 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2564 3152 3236 928 Size: 971 665 588 84 4020 Fragments arranged by size: 4020 971 665 588 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2819 2853 4519 4553 1903 Size: 34 882 34 1666 34 3678 Fragments arranged by size: 3678 1666 882 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4220 5430 4220 Size: 1210 5118 BclI T'GATC_A Cuts at: 1548 1548 Size: 6328 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4939 136 Size: 107 122 71 631 605 3267 1525 Fragments arranged by size: 3267 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6323 6323 Size: 6328 BlpI GC'TnA_GC Cuts at: 3745 3745 Size: 6328 Bme1580I G_kGCm'C Cuts at: 1790 2662 4389 5545 1790 Size: 872 1727 1156 2573 Fragments arranged by size: 2573 1727 1156 872 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6328 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4059 5017 442 Size: 416 53 83 399 24 2642 958 1753 Fragments arranged by size: 2642 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6328 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2277 4988 1239 Size: 1038 2711 2579 Fragments arranged by size: 2711 2579 1038 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4453 5318 5616 4453 Size: 865 298 5165 Fragments arranged by size: 5165 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3280 4991 882 Size: 617 1781 1711 2219 Fragments arranged by size: 2219 1781 1711 617 BsaAI yAC'GTr Cuts at: 493 1204 3625 4119 493 Size: 711 2421 494 2702 Fragments arranged by size: 2702 2421 711 494 BsaBI GATnn'nnATC Cuts at: 6322 6322 Size: 6328 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4135 4517 5152 275 Size: 53 83 186 936 2602 382 635 1451 Fragments arranged by size: 2602 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2690 4761 5159 5433 5580 5991 2690 Size: 2071 398 274 147 411 3027 Fragments arranged by size: 3027 2071 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2275 2305 2517 2547 1178 Size: 30 165 30 872 30 212 30 4959 Fragments arranged by size: 4959 872 212 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6328 BseYI C'CCAG_C Cuts at: 5531 6027 5531 Size: 496 5832 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5755 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3778 4539 5567 1540 Size: 275 186 1777 761 1028 2301 Fragments arranged by size: 2301 1777 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2662 2742 4389 4474 5545 2662 Size: 80 1647 85 1071 3445 Fragments arranged by size: 3445 1647 1071 85 80 BsmI GAATG_Cn' Cuts at: 2399 3270 3580 3839 3932 2399 Size: 871 310 259 93 4795 Fragments arranged by size: 4795 871 310 259 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2662 2742 3647 4389 4474 5545 1391 Size: 399 872 80 905 742 85 1071 2174 Fragments arranged by size: 2174 1071 905 872 742 399 85 80 BspEI T'CCGG_A Cuts at: 5991 5991 Size: 6328 BspHI T'CATG_A Cuts at: 4217 5947 4217 Size: 1730 4598 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3683 844 Size: 2839 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4215 1394 Size: 429 2392 3507 Fragments arranged by size: 3507 2392 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4823 4997 65 Size: 1183 3575 174 1396 Fragments arranged by size: 3575 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2690 4972 5159 1700 Size: 110 880 2282 187 2869 Fragments arranged by size: 2869 2282 880 187 110 BsrGI T'GTAC_A Cuts at: 96 2909 96 Size: 2813 3515 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6328 BssSI C'ACGA_G Cuts at: 3195 4388 5400 3195 Size: 1193 1012 4123 Fragments arranged by size: 4123 1193 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6328 BstBI TT'CG_AA Cuts at: 2007 5132 2007 Size: 3125 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6328 BstYI r'GATC_y Cuts at: 1972 2092 3658 4025 4410 4427 5879 5965 5977 Size: 120 1566 367 385 17 1452 86 12 Cuts at: 5977 6323 1972 Size: 346 1977 Fragments arranged by size: 1977 1566 1452 385 367 346 120 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2029 513 Size: 552 957 7 4812 Fragments arranged by size: 4812 957 552 7 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5589 Fragments arranged by size: 5589 683 56 BtsI GCAGTG_nn' Cuts at: 3844 4638 4658 3844 Size: 794 20 5514 Fragments arranged by size: 5514 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6328 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6293 DraI TTT'AAA Cuts at: 3632 3632 Size: 6328 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5335 809 Size: 507 4019 1802 Fragments arranged by size: 4019 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2055 3775 4664 Size: 54 1425 50 275 18 225 1720 889 Cuts at: 4664 6293 8 Size: 1629 43 Fragments arranged by size: 1720 1629 1425 889 275 225 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3775 1537 Size: 275 1963 4090 Fragments arranged by size: 4090 1963 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3297 4258 1353 Size: 1944 961 3423 Fragments arranged by size: 3423 1944 961 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4915 5289 5435 387 Size: 1064 138 3326 374 146 1280 Fragments arranged by size: 3326 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2277 2348 4391 4988 5775 6043 1239 Size: 393 645 71 2043 597 787 268 1524 Fragments arranged by size: 2043 1524 787 645 597 393 268 71 EcoO109I rG'GnC_Cy Cuts at: 2159 2159 Size: 6328 EcoRI G'AATT_C Cuts at: 2084 2598 2850 3522 3637 4015 2084 Size: 514 252 672 115 378 4397 Fragments arranged by size: 4397 672 514 378 252 115 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3586 3586 Size: 6328 FspI TGC'GCA Cuts at: 4834 4834 Size: 6328 HaeII r_GCGC'y Cuts at: 1678 5475 1678 Size: 3797 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 4969 5001 5043 Size: 32 135 32 677 32 2688 32 42 Cuts at: 5043 5075 1373 Size: 32 2626 Fragments arranged by size: 2688 2626 677 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2588 3672 3918 669 Size: 988 281 650 1084 246 3079 Fragments arranged by size: 3079 1084 988 650 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6328 HpaI GTT'AAC Cuts at: 3918 3918 Size: 6328 KpnI G_GTAC'C Cuts at: 3653 3653 Size: 6328 MfeI C'AATT_G Cuts at: 3927 3927 Size: 6328 MluI A'CGCG_T Cuts at: 6204 6204 Size: 6328 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3206 5442 5626 1305 Size: 1901 2236 184 2007 Fragments arranged by size: 2236 2007 1901 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2057 10 Size: 54 1425 343 225 4281 Fragments arranged by size: 4281 1425 343 225 54 MslI CAynn'nnrTG Cuts at: 518 1166 2326 2607 2691 2775 2859 3027 3111 Size: 648 1160 281 84 84 84 168 84 Cuts at: 3111 3195 3279 3363 3447 3531 4286 4645 4804 Size: 84 84 84 84 84 755 359 159 Cuts at: 4804 518 Size: 2042 Fragments arranged by size: 2042 1160 755 648 359 281 168 159 84 84 84 84 84 84 84 84 84 MspA1I CmG'CkG Cuts at: 1606 3744 4421 5569 5814 1606 Size: 2138 677 1148 245 2120 Fragments arranged by size: 2138 2120 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2029 513 Size: 552 957 7 4812 Fragments arranged by size: 4812 957 552 7 NdeI CA'TA_TG Cuts at: 387 387 Size: 6328 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6328 NotI GC'GGCC_GC Cuts at: 3775 3775 Size: 6328 NspI r_CATG'y Cuts at: 1132 1229 3686 5231 1132 Size: 97 2457 1545 2229 Fragments arranged by size: 2457 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6328 PciI A'CATG_T Cuts at: 5227 5227 Size: 6328 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6328 PfoI T'CCnGG_A Cuts at: 2088 2088 Size: 6328 PmeI GTTT'AAAC Cuts at: 3632 3632 Size: 6328 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4397 4429 5910 5942 4397 Size: 32 1481 32 4783 Fragments arranged by size: 4783 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2159 2159 Size: 6328 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6328 PsiI TTA'TAA Cuts at: 2622 3898 2622 Size: 1276 5052 PstI C_TGCA'G Cuts at: 830 3680 830 Size: 2850 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6328 SalI G'TCGA_C Cuts at: 1936 3670 1936 Size: 1734 4594 SbfI CC_TGCA'GG Cuts at: 3680 3680 Size: 6328 ScaI AGT'ACT Cuts at: 4576 6288 4576 Size: 1712 4616 SfcI C'TryA_G Cuts at: 826 1045 2137 2356 3676 4811 5492 5683 826 Size: 219 1092 219 1320 1135 681 191 1471 Fragments arranged by size: 1471 1320 1135 1092 681 219 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6328 SgrAI Cr'CCGG_yG Cuts at: 1700 2690 1700 Size: 990 5338 SmaI CCC'GGG Cuts at: 1872 3655 1872 Size: 1783 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4432 5333 5595 820 Size: 197 926 2489 901 262 1553 Fragments arranged by size: 2489 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3625 4119 493 Size: 3132 494 2702 Fragments arranged by size: 3132 2702 494 SpeI A'CTAG_T Cuts at: 152 3169 152 Size: 3017 3311 SphI G_CATG'C Cuts at: 1132 3686 1132 Size: 2554 3774 SspI AAT'ATT Cuts at: 5 52 4252 5 Size: 47 4200 2081 Fragments arranged by size: 4200 2081 47 StuI AGG'CCT Cuts at: 1517 2645 2729 2813 3317 3569 1517 Size: 1128 84 84 504 252 4276 Fragments arranged by size: 4276 1128 504 252 84 84 StyI C'CwwG_G Cuts at: 513 1065 2022 2029 3767 513 Size: 552 957 7 1738 3074 Fragments arranged by size: 3074 1738 957 552 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4355 4372 4525 4710 5155 325 Size: 1255 2775 17 153 185 445 1498 Fragments arranged by size: 2775 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2821 2909 3007 Size: 274 80 33 51 1443 844 88 98 Cuts at: 3007 4574 6286 96 Size: 1567 1712 138 Fragments arranged by size: 1712 1567 1443 844 274 138 98 88 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2111 2143 2517 2549 6288 6320 2111 Size: 32 374 32 3739 32 2119 Fragments arranged by size: 3739 2119 374 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6328 XbaI T'CTAG_A Cuts at: 3664 3664 Size: 6328 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6328 XmaI C'CCGG_G Cuts at: 1870 3653 1870 Size: 1783 4545 XmnI GAAnn'nnTTC Cuts at: 2981 4457 2981 Size: 1476 4852 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4136 276 Size: 53 83 186 936 2602 2468 Fragments arranged by size: 2602 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI