(Circular) (Six-base) MAPSORT of: pFN21ASDA1518.seq Check: 209 from: 1 to: 7282 pFN21ASDA1518 7282 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2092 2626 2092 Size: 534 6748 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2270 5092 278 Size: 53 83 186 936 734 2822 2468 Fragments arranged by size: 2822 2468 936 734 186 83 53 AccI GT'mk_AC Cuts at: 1937 4625 1937 Size: 2688 4594 Acc65I G'GTAC_C Cuts at: 4603 4603 Size: 7282 AclI AA'CG_TT Cuts at: 1563 5409 5782 1563 Size: 3846 373 3063 Fragments arranged by size: 3846 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4175 4181 5345 6729 6997 1632 Size: 2543 6 1164 1384 268 1917 Fragments arranged by size: 2543 1917 1384 1164 268 6 AflII C'TTAA_G Cuts at: 820 1017 2711 820 Size: 197 1694 5391 Fragments arranged by size: 5391 1694 197 AflIII A'CryG_T Cuts at: 1203 2203 6181 7158 1203 Size: 1000 3978 977 1327 Fragments arranged by size: 3978 1327 1000 977 AgeI A'CCGG_T Cuts at: 2551 6113 2551 Size: 3562 3720 AhdI GACnn_n'nnGTC Cuts at: 6011 6011 Size: 7282 AleI CACnn'nnGTG Cuts at: 1166 2552 3887 1166 Size: 1386 1335 4561 Fragments arranged by size: 4561 1386 1335 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2061 1184 Size: 877 6405 AlwNI CAG_nnn'CTG Cuts at: 2035 2929 3062 3091 4078 5025 6597 2035 Size: 894 133 29 987 947 1572 2720 Fragments arranged by size: 2720 1572 987 947 894 133 29 ApaI G_GGCC'C Cuts at: 3085 3121 3447 3085 Size: 36 326 6920 Fragments arranged by size: 6920 326 36 ApaLI G'TGCA_C Cuts at: 5339 6495 5339 Size: 1156 6126 ApoI r'AATT_y Cuts at: 1493 4591 4817 4969 7078 1493 Size: 3098 226 152 2109 1697 Fragments arranged by size: 3098 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5836 160 Size: 872 4804 1606 Fragments arranged by size: 4804 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 AvaI C'yCGr_G Cuts at: 1870 1943 2426 3310 3717 3935 4607 1870 Size: 73 483 884 407 218 672 4545 Fragments arranged by size: 4545 884 672 483 407 218 73 BamHI G'GATC_C Cuts at: 4612 4979 4612 Size: 367 6915 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2554 2571 2603 2825 3065 Size: 325 217 627 767 17 32 222 240 Cuts at: 3065 3434 3951 4603 6058 618 Size: 369 517 652 1455 1842 Fragments arranged by size: 1842 1455 767 652 627 517 369 325 240 222 217 32 17 BanII G_rGCy'C Cuts at: 1391 2237 2462 2766 3085 3121 3149 3378 3393 Size: 846 225 304 319 36 28 229 15 Cuts at: 3393 3447 4553 4601 1391 Size: 54 1106 48 4072 Fragments arranged by size: 4072 1106 846 319 304 229 225 54 48 36 28 15 BbeI G_GCGC'C Cuts at: 3069 3069 Size: 7282 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3291 4169 928 Size: 971 1392 878 4041 Fragments arranged by size: 4041 1392 971 878 BbvCI CC'TCA_GC Cuts at: 3113 3744 3113 Size: 631 6651 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5473 5507 1903 Size: 34 3536 34 3678 Fragments arranged by size: 3678 3536 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2998 5174 6384 2998 Size: 2176 1210 3896 Fragments arranged by size: 3896 2176 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7282 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2951 3136 3397 Size: 107 122 71 631 605 1279 185 261 Cuts at: 3397 3818 4232 4298 4307 5893 136 Size: 421 414 66 9 1586 1525 Fragments arranged by size: 1586 1525 1279 631 605 421 414 261 185 122 107 71 66 9 BglII A'GATC_T Cuts at: 7277 7277 Size: 7282 BlpI GC'TnA_GC Cuts at: 3791 4699 3791 Size: 908 6374 Bme1580I G_kGCm'C Cuts at: 1790 2574 2608 2828 3085 3121 3191 3198 3366 Size: 784 34 220 257 36 70 7 168 Cuts at: 3366 3437 3447 4407 5343 6499 1790 Size: 71 10 960 936 1156 2573 Fragments arranged by size: 2573 1156 960 936 784 257 220 168 71 70 36 34 10 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7282 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2466 2556 2792 Size: 416 53 83 399 24 1049 90 236 Cuts at: 2792 3185 5013 5971 442 Size: 393 1828 958 1753 Fragments arranged by size: 1828 1753 1049 958 416 399 393 236 90 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7282 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3343 3601 4464 3343 Size: 258 863 6161 Fragments arranged by size: 6161 863 258 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2384 2642 2648 2663 2828 2918 3113 3503 Size: 1145 258 6 15 165 90 195 390 Cuts at: 3503 3550 5942 1239 Size: 47 2392 2579 Fragments arranged by size: 2579 2392 1145 390 258 195 165 90 47 15 6 Bpu10I CC'TnA_GC Cuts at: 2231 2940 3113 3662 3744 2231 Size: 709 173 549 82 5769 Fragments arranged by size: 5769 709 549 173 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2147 2894 5407 6272 6570 2147 Size: 747 2513 865 298 2859 Fragments arranged by size: 2859 2513 865 747 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2120 2360 2804 2894 5945 882 Size: 617 621 240 444 90 3051 2219 Fragments arranged by size: 3051 2219 621 617 444 240 90 BsaAI yAC'GTr Cuts at: 493 1204 2041 2170 2584 3997 4579 5073 493 Size: 711 837 129 414 1413 582 494 2702 Fragments arranged by size: 2702 1413 837 711 582 494 414 129 BsaBI GATnn'nnATC Cuts at: 3239 7276 3239 Size: 4037 3245 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2267 3066 5089 5471 Size: 53 83 186 936 734 799 2023 382 Cuts at: 5471 6106 275 Size: 635 1451 Fragments arranged by size: 2023 1451 936 799 734 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2551 3507 5715 6113 6387 6534 6945 2551 Size: 956 2208 398 274 147 411 2888 Fragments arranged by size: 2888 2208 956 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3170 3200 1178 Size: 30 165 30 1767 30 5260 Fragments arranged by size: 5260 1767 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2645 3437 3497 3526 3785 3788 1186 Size: 1459 792 60 29 259 3 4680 Fragments arranged by size: 4680 1459 792 259 60 29 3 BseYI C'CCAG_C Cuts at: 2071 2904 2961 3657 4406 6485 6981 2071 Size: 833 57 696 749 2079 496 2372 Fragments arranged by size: 2372 2079 833 749 696 496 57 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2021 2234 4019 4091 1163 Size: 573 285 213 1785 72 4354 Fragments arranged by size: 4354 1785 573 285 213 72 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2335 2481 4732 5493 6521 1540 Size: 275 186 334 146 2251 761 1028 2301 Fragments arranged by size: 2301 2251 1028 761 334 275 186 146 BsiHKAI G_wGCw'C Cuts at: 2237 2288 2362 2658 2679 2766 3353 4368 4445 Size: 51 74 296 21 87 587 1015 77 Cuts at: 4445 4523 5343 5428 6499 2237 Size: 78 820 85 1071 3020 Fragments arranged by size: 3020 1071 1015 820 587 296 87 85 78 77 74 51 21 BsmI GAATG_Cn' Cuts at: 4793 4886 4793 Size: 93 7189 BsmBI CGTCTCn'nnnn_ Cuts at: 2429 2429 Size: 7282 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2237 2288 2362 2462 2574 2608 2658 Size: 399 447 51 74 100 112 34 50 Cuts at: 2658 2679 2766 2828 3085 3121 3149 3191 3198 Size: 21 87 62 257 36 28 42 7 Cuts at: 3198 3353 3366 3378 3393 3437 3447 4368 4407 Size: 155 13 12 15 44 10 921 39 Cuts at: 4407 4445 4523 4553 4601 5343 5428 6499 1391 Size: 38 78 30 48 742 85 1071 2174 Fragments arranged by size: 2174 1071 921 742 447 399 257 155 112 100 87 85 78 74 62 51 50 48 44 42 39 38 36 34 30 28 21 15 13 12 10 7 BspEI T'CCGG_A Cuts at: 6945 6945 Size: 7282 BspHI T'CATG_A Cuts at: 3468 5171 6901 3468 Size: 1703 1730 3849 Fragments arranged by size: 3849 1730 1703 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2092 2626 4637 844 Size: 1248 534 2011 3489 Fragments arranged by size: 3489 2011 1248 534 BsrBI CCG'CTC Cuts at: 1394 1823 3862 5169 1394 Size: 429 2039 1307 3507 Fragments arranged by size: 3507 2039 1307 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3132 5777 5951 65 Size: 1183 1884 2645 174 1396 Fragments arranged by size: 2645 1884 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2450 2480 2551 3437 3696 5926 6113 Size: 110 640 30 71 886 259 2230 187 Cuts at: 6113 1700 Size: 2869 Fragments arranged by size: 2869 2230 886 640 259 187 110 71 30 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7282 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7282 BssSI C'ACGA_G Cuts at: 5342 6354 5342 Size: 1012 6270 BstAPI GCAn_nnn'nTGC Cuts at: 794 2929 4078 794 Size: 2135 1149 3998 Fragments arranged by size: 3998 2135 1149 BstBI TT'CG_AA Cuts at: 2007 6086 2007 Size: 4079 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7282 BstXI CCAn_nnnn'nTGG Cuts at: 2455 2455 Size: 7282 BstYI r'GATC_y Cuts at: 1972 4612 4979 5364 5381 6833 6919 6931 7277 Size: 2640 367 385 17 1452 86 12 346 Cuts at: 7277 1972 Size: 1977 Fragments arranged by size: 2640 1977 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2121 2245 2335 2371 2387 2528 Size: 552 957 99 124 90 36 16 141 Cuts at: 2528 3029 3498 4065 4267 4341 513 Size: 501 469 567 202 74 3454 Fragments arranged by size: 3454 957 567 552 501 469 202 141 124 99 90 74 36 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3273 3991 4449 487 Size: 683 56 2047 718 458 3320 Fragments arranged by size: 3320 2047 718 683 458 56 BtsI GCAGTG_nn' Cuts at: 2217 3738 4798 5592 5612 2217 Size: 1521 1060 794 20 3887 Fragments arranged by size: 3887 1521 1060 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7282 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2278 2313 529 Size: 35 1714 35 5498 Fragments arranged by size: 5498 1714 35 35 DraI TTT'AAA Cuts at: 4586 4586 Size: 7282 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2993 3998 4091 6289 809 Size: 507 1677 1005 93 2198 1802 Fragments arranged by size: 2198 1802 1677 1005 507 93 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2332 2439 2478 Size: 54 1425 50 275 18 502 107 39 Cuts at: 2478 2975 3048 3645 3684 4152 4257 4369 4398 Size: 497 73 597 39 468 105 112 29 Cuts at: 4398 4729 5618 7247 8 Size: 331 889 1629 43 Fragments arranged by size: 1629 1425 889 597 502 497 468 331 275 112 107 105 73 54 50 43 39 39 29 18 EagI C'GGCC_G Cuts at: 1537 1812 2332 2478 4729 1537 Size: 275 520 146 2251 4090 Fragments arranged by size: 4090 2251 520 275 146 EarI CTCTTCn'nnn_ Cuts at: 1353 3341 4202 4566 5212 1353 Size: 1988 861 364 646 3423 Fragments arranged by size: 3423 1988 861 646 364 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5869 6243 6389 387 Size: 1064 138 4280 374 146 1280 Fragments arranged by size: 4280 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2235 2764 2235 Size: 529 6753 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2384 2642 2648 2663 2828 2918 3113 Size: 393 752 258 6 15 165 90 195 Cuts at: 3113 3503 3550 4175 4181 5345 5942 6729 6997 Size: 390 47 625 6 1164 597 787 268 Cuts at: 6997 1239 Size: 1524 Fragments arranged by size: 1524 1164 787 752 625 597 393 390 268 258 195 165 90 47 15 6 6 EcoO109I rG'GnC_Cy Cuts at: 2032 2061 2101 2794 3117 3118 3444 3613 2032 Size: 29 40 693 323 1 326 169 5701 Fragments arranged by size: 5701 693 326 323 169 40 29 1 EcoRI G'AATT_C Cuts at: 4591 4969 4591 Size: 378 6904 FspI TGC'GCA Cuts at: 5788 5788 Size: 7282 HaeII r_GCGC'y Cuts at: 1678 2221 2921 3069 6429 1678 Size: 543 700 148 3360 2531 Fragments arranged by size: 3360 2531 700 543 148 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3539 3571 3737 3769 3791 Size: 32 135 32 1967 32 166 32 22 Cuts at: 3791 3823 4322 4354 4472 4504 5923 5955 5997 Size: 32 499 32 118 32 1419 32 42 Cuts at: 5997 6029 1373 Size: 32 2626 Fragments arranged by size: 2626 1967 1419 499 166 135 118 42 32 32 32 32 32 32 32 32 32 22 HincII GTy'rAC Cuts at: 669 1657 1938 3535 4003 4318 4626 4872 669 Size: 988 281 1597 468 315 308 246 3079 Fragments arranged by size: 3079 1597 988 468 315 308 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7282 HpaI GTT'AAC Cuts at: 4872 4872 Size: 7282 KasI G'GCGC_C Cuts at: 3065 3065 Size: 7282 KpnI G_GTAC'C Cuts at: 4607 4607 Size: 7282 MfeI C'AATT_G Cuts at: 4881 4881 Size: 7282 MluI A'CGCG_T Cuts at: 2203 7158 2203 Size: 4955 2327 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6396 6580 1305 Size: 5091 184 2007 Fragments arranged by size: 5091 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2977 3050 4154 10 Size: 54 1425 343 1145 73 1104 3138 Fragments arranged by size: 3138 1425 1145 1104 343 73 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 3887 5240 5599 5758 518 Size: 648 1386 1335 1353 359 159 2042 Fragments arranged by size: 2042 1386 1353 1335 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2373 2389 2626 2744 3062 3079 3200 3683 Size: 767 16 237 118 318 17 121 483 Cuts at: 3683 3712 4078 4089 4410 4698 5375 6523 6768 Size: 29 366 11 321 288 677 1148 245 Cuts at: 6768 1606 Size: 2120 Fragments arranged by size: 2120 1148 767 677 483 366 321 318 288 245 237 121 118 29 17 16 11 NaeI GCC'GGC Cuts at: 2482 3698 2482 Size: 1216 6066 NarI GG'CG_CC Cuts at: 3066 3066 Size: 7282 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5773 Fragments arranged by size: 5773 957 552 NdeI CA'TA_TG Cuts at: 387 3222 387 Size: 2835 4447 NgoMIV G'CCGG_C Cuts at: 2480 3696 2480 Size: 1216 6066 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7282 NotI GC'GGCC_GC Cuts at: 4729 4729 Size: 7282 NruI TCG'CGA Cuts at: 4434 4434 Size: 7282 NspI r_CATG'y Cuts at: 1132 1229 2857 4640 6185 1132 Size: 97 1628 1783 1545 2229 Fragments arranged by size: 2229 1783 1628 1545 97 PasI CC'CwG_GG Cuts at: 1348 2057 1348 Size: 709 6573 PciI A'CATG_T Cuts at: 6181 6181 Size: 7282 PflMI CCAn_nnn'nTGG Cuts at: 1108 3091 3172 3526 1108 Size: 1983 81 354 4864 Fragments arranged by size: 4864 1983 354 81 PfoI T'CCnGG_A Cuts at: 2679 2679 Size: 7282 PmeI GTTT'AAAC Cuts at: 4586 4586 Size: 7282 PmlI CAC'GTG Cuts at: 2041 2584 2041 Size: 543 6739 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5351 5383 6864 6896 5351 Size: 32 1481 32 5737 Fragments arranged by size: 5737 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2032 2061 2101 2794 2032 Size: 29 40 693 6520 Fragments arranged by size: 6520 693 40 29 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7282 PsiI TTA'TAA Cuts at: 4852 4852 Size: 7282 PspOMI G'GGCC_C Cuts at: 3081 3117 3443 3081 Size: 36 326 6920 Fragments arranged by size: 6920 326 36 PstI C_TGCA'G Cuts at: 830 2925 2934 3205 3673 3897 4634 830 Size: 2095 9 271 468 224 737 3478 Fragments arranged by size: 3478 2095 737 468 271 224 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7282 PvuII CAG'CTG Cuts at: 2626 3712 4078 4410 2626 Size: 1086 366 332 5498 Fragments arranged by size: 5498 1086 366 332 RsrII CG'GwC_CG Cuts at: 2995 2995 Size: 7282 SacI G_AGCT'C Cuts at: 2237 2766 2237 Size: 529 6753 SacII CC_GC'GG Cuts at: 2374 2390 2374 Size: 16 7266 SalI G'TCGA_C Cuts at: 1936 4624 1936 Size: 2688 4594 SanDI GG'GwC_CC Cuts at: 2061 2061 Size: 7282 SapI GCTCTTCn'nnn_ Cuts at: 3341 4202 3341 Size: 861 6421 SbfI CC_TGCA'GG Cuts at: 3673 4634 3673 Size: 961 6321 ScaI AGT'ACT Cuts at: 5530 7242 5530 Size: 1712 5570 SexAI A'CCwGG_T Cuts at: 3954 3954 Size: 7282 SfcI C'TryA_G Cuts at: 826 1045 2921 2930 3201 3669 3893 4126 4630 Size: 219 1876 9 271 468 224 233 504 Cuts at: 4630 5765 6446 6637 826 Size: 1135 681 191 1471 Fragments arranged by size: 1876 1471 1135 681 504 468 271 233 224 219 191 9 SfoI GGC'GCC Cuts at: 3067 3067 Size: 7282 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7282 SgrAI Cr'CCGG_yG Cuts at: 1700 2551 3437 1700 Size: 851 886 5545 Fragments arranged by size: 5545 886 851 SmaI CCC'GGG Cuts at: 1872 3312 4609 1872 Size: 1440 1297 4545 Fragments arranged by size: 4545 1440 1297 SmlI C'TyrA_G Cuts at: 820 1017 1943 2162 2426 2711 2909 3717 5386 Size: 197 926 219 264 285 198 808 1669 Cuts at: 5386 6287 6549 820 Size: 901 262 1553 Fragments arranged by size: 1669 1553 926 901 808 285 264 262 219 198 197 SnaBI TAC'GTA Cuts at: 493 4579 5073 493 Size: 4086 494 2702 Fragments arranged by size: 4086 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7282 SphI G_CATG'C Cuts at: 1132 2857 4640 1132 Size: 1725 1783 3774 Fragments arranged by size: 3774 1783 1725 SspI AAT'ATT Cuts at: 5 52 5206 5 Size: 47 5154 2081 Fragments arranged by size: 5154 2081 47 StuI AGG'CCT Cuts at: 1517 2972 3139 3273 3667 1517 Size: 1455 167 134 394 5132 Fragments arranged by size: 5132 1455 394 167 134 StyI C'CwwG_G Cuts at: 513 1065 2022 2097 2883 4461 4721 513 Size: 552 957 75 786 1578 260 3074 Fragments arranged by size: 3074 1578 957 786 552 260 75 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2461 3383 3395 5309 5326 5479 5664 Size: 1255 881 922 12 1914 17 153 185 Cuts at: 5664 6109 325 Size: 445 1498 Fragments arranged by size: 1914 1498 1255 922 881 445 185 153 17 12 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2704 5528 7240 Size: 274 80 33 51 1443 727 2824 1712 Cuts at: 7240 96 Size: 138 Fragments arranged by size: 2824 1712 1443 727 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2073 2105 3141 3170 3173 3202 7242 7274 2073 Size: 32 1036 29 3 29 4040 32 2081 Fragments arranged by size: 4040 2081 1036 32 32 29 29 3 Tth111I GACn'n_nGTC Cuts at: 1316 4283 1316 Size: 2967 4315 XbaI T'CTAG_A Cuts at: 4618 4618 Size: 7282 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4063 4063 Size: 7282 XhoI C'TCGA_G Cuts at: 1943 2426 3717 1943 Size: 483 1291 5508 Fragments arranged by size: 5508 1291 483 XmaI C'CCGG_G Cuts at: 1870 3310 4607 1870 Size: 1440 1297 4545 Fragments arranged by size: 4545 1440 1297 XmnI GAAnn'nnTTC Cuts at: 5411 5411 Size: 7282 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2268 5090 276 Size: 53 83 186 936 734 2822 2468 Fragments arranged by size: 2822 2468 936 734 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NsiI PacI PspXI PsrI SfiI SrfI SwaI