Restriction map for
pFN21ASDA1499
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AccII AclI AcsI AflII AgeI
AhdI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AviII AvrII BbeI BbsI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsiEI BsiWI BsmI Bsp1407I
BspDI BspHI BsrBI BsrGI BssHII
BssSI Bst1107I BstBI BstUI BstZ17I
Bsu36I CelII ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoRI EcoRV EcoT22I FauI
FseI FspI HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MunI MvnI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AatII 270 GACGTC GACGT^C
AccI 1285 GTAGAC GT^[AC][GT]AC
AccIII 503 TCCGGA T^CCGGA
Alw44I 415 GTGCAC G^TGCAC
ApaI 250 GGGCCC GGGCC^C
ApaLI 415 GTGCAC G^TGCAC
AspI 940 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1463 TGGCCA TGG^CCA
BbrPI 1835 CACGTG CAC^GTG
BbvCI 339 CCTCAGC CCTCAGC(-5/-2)
BcgI 888 CGATGAAATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1503 GGATAC GTATCC(6/5)
BclI 330 TGATCA T^GATCA
BglI 389 GCCGCCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI 374 GAGACC GGTCTC(1/5)
BseAI 503 TCCGGA T^CCGGA
BspEI 503 TCCGGA T^CCGGA
BspMI 1785 GCAGGT ACCTGC(4/8)
BsrDI 1798 GCAATG GCAATG(2/0)
BstAPI 1254 GCAGTACGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1539 GGTCACC G^GT[ACGT]ACC
BstPI 1539 GGTCACC G^GT[ACGT]ACC
DraII 250 GGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 1696 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1129 GACAACCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 912 CCTGCGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 250 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1539 GGTCACC G^GT[ACGT]ACC
FbaI 330 TGATCA T^GATCA
MluNI 1463 TGGCCA TGG^CCA
MroI 503 TCCGGA T^CCGGA
MscI 1463 TGGCCA TGG^CCA
MseI 569 TTAA T^TAA
MslI 380 CACAGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 1064 GCCGGC GCC^GGC
NgoMIV 1064 GCCGGC G^CCGGC
PflFI 940 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1455 CCATAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1835 CACGTG CAC^GTG
PmlI 1835 CACGTG CAC^GTG
SacI 1612 GAGCTC GAGCT^C
SexAI 1154 ACCAGGT A^CC[AT]GGT
Tru9I 569 TTAA T^TAA
Tsp45I 1540 GTCAC ^GT[GC]AC
Tth111I 940 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1455 CCATAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 429 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 967 GGTACC G^GTACC
1151 GGTACC
AcyI 270 GACGTC G[GA]^CG[CT]C
1213 GACGCC
AflIII 482 ACATGT A^C[GA][CT]GT
1058 ACATGT
Asp718I 967 GGTACC G^GTACC
1151 GGTACC
AvaI 237 CTCGAG C^[CT]CG[GA]G
463 CTCGAG
BanII 250 GGGCCC G[GA]GC[CT]^C
1612 GAGCTC
BfaI 430 CTAG C^TAG
1244 CTAG
BsaAI 1258 TACGTG [CT]AC^GT[GA]
1835 CACGTG
BsaHI 270 GACGTC G[GA]^CG[CT]C
1213 GACGCC
BsaWI 157 ACCGGA [AT]^CCGG[AT]
503 TCCGGA
BseRI 174 CTCCTC GAGGAG(10/8)
1609 GAGGAG
BsgI 1729 CTGCAC GTGCAG(16/14)
1665 GTGCAG
BsmBI 945 CGTCTC CGTCTC(1/5)
268 GAGACG
BsmFI 30 GTCCC GGGAC(10/14)
424 GTCCC
BsoBI 237 CTCGAG C^[CT]CG[GA]G
463 CTCGAG
BspLU11I 482 ACATGT A^CATGT
1058 ACATGT
BsrFI 1064 GCCGGC [GA]^CCGG[CT]
1746 GCCGGT
CfoI 302 GCGC GCG^C
632 GCGC
Cfr10I 1064 GCCGGC [GA]^CCGG[CT]
1746 GCCGGT
EarI 1015 GAAGAG CTCTTC(1/4)
1629 GAAGAG
HaeII 301 GGCGCT [GA]GCGC^[CT]
631 AGCGCC
HgaI 1213 GACGC GACGC(5/10)
422 GCGTC
HhaI 302 GCGC GCG^C
632 GCGC
Hin1I 270 GACGTC G[GA]^CG[CT]C
1213 GACGCC
HinP1I 302 GCGC G^CGC
632 GCGC
KpnI 967 GGTACC GGTAC^C
1151 GGTACC
Ksp632I 1015 GAAGAG CTCTTC(1/4)
1629 GAAGAG
MaeI 430 CTAG C^TAG
1244 CTAG
MaeIII 1102 GTTAC ^GT[ACGT]AC
1540 GTCAC
PaeR7I 237 CTCGAG C^TCGAG
463 CTCGAG
PleI 1815 GACTC GAGTC(4/5)
1240 GAGTC
TfiI 687 GAATC G^A[AT]TC
1761 GATTC
Tsp509I 18 AATT ^AATT
894 AATT
XhoI 237 CTCGAG C^TCGAG
463 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BamHI BanI BbvI BcnI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TseI TspRI TthHB8I XcmI XhoII