(Circular) (Six-base) MAPSORT of: pFN21ASDA1486.seq Check: 8563 from: 1 to: 6688 pFN21ASDA1486 6688 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2684 3893 4498 278 Size: 53 83 186 936 1148 1209 605 2468 Fragments arranged by size: 2468 1209 1148 936 605 186 83 53 AccI GT'mk_AC Cuts at: 1937 2823 3275 3796 3887 4031 1937 Size: 886 452 521 91 144 4594 Fragments arranged by size: 4594 886 521 452 144 91 Acc65I G'GTAC_C Cuts at: 3955 4009 3955 Size: 54 6634 AclI AA'CG_TT Cuts at: 1563 4815 5188 1563 Size: 3252 373 3063 Fragments arranged by size: 3252 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2562 2773 3417 4751 6135 6403 1632 Size: 930 211 644 1334 1384 268 1917 Fragments arranged by size: 1917 1384 1334 930 644 268 211 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6491 AflIII A'CryG_T Cuts at: 1203 3126 5587 6564 1203 Size: 1923 2461 977 1327 Fragments arranged by size: 2461 1923 1327 977 AgeI A'CCGG_T Cuts at: 5519 5519 Size: 6688 AhdI GACnn_n'nnGTC Cuts at: 5417 5417 Size: 6688 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6688 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3611 3956 1184 Size: 2427 345 3916 Fragments arranged by size: 3916 2427 345 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3280 3312 3645 3677 3280 Size: 32 333 32 6291 Fragments arranged by size: 6291 333 32 32 AlwNI CAG_nnn'CTG Cuts at: 2383 2418 4431 6003 2383 Size: 35 2013 1572 3068 Fragments arranged by size: 3068 2013 1572 35 ApaI G_GGCC'C Cuts at: 2673 2673 Size: 6688 ApaLI G'TGCA_C Cuts at: 4745 5901 4745 Size: 1156 5532 ApoI r'AATT_y Cuts at: 1493 3997 4223 4375 6484 1493 Size: 2504 226 152 2109 1697 Fragments arranged by size: 2504 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5242 160 Size: 872 4210 1606 Fragments arranged by size: 4210 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 AvaI C'yCGr_G Cuts at: 1870 1943 2151 2735 3517 4013 1870 Size: 73 208 584 782 496 4545 Fragments arranged by size: 4545 782 584 496 208 73 BamHI G'GATC_C Cuts at: 4018 4385 4018 Size: 367 6321 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2923 3955 4009 5464 618 Size: 325 217 627 1136 1032 54 1455 1842 Fragments arranged by size: 1842 1455 1136 1032 627 325 217 54 BanII G_rGCy'C Cuts at: 1391 2673 2751 3428 3551 3821 4007 1391 Size: 1282 78 677 123 270 186 4072 Fragments arranged by size: 4072 1282 677 270 186 123 78 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2067 2227 3720 928 Size: 971 168 160 1493 3896 Fragments arranged by size: 3896 1493 971 168 160 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2623 2657 4879 4913 1903 Size: 34 686 34 2222 34 3678 Fragments arranged by size: 3678 2222 686 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4580 5790 4580 Size: 1210 5478 BclI T'GATC_A Cuts at: 1548 1548 Size: 6688 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5299 136 Size: 107 122 71 631 605 3627 1525 Fragments arranged by size: 3627 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6683 6683 Size: 6688 BlpI GC'TnA_GC Cuts at: 2474 2630 4105 2474 Size: 156 1475 5057 Fragments arranged by size: 5057 1475 156 Bme1580I G_kGCm'C Cuts at: 1790 2402 2673 4749 5905 1790 Size: 612 271 2076 1156 2573 Fragments arranged by size: 2573 2076 1156 612 271 BmgBI CAC'GTC Cuts at: 1318 3181 1318 Size: 1863 4825 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3286 4419 5377 Size: 416 53 83 399 24 1869 1133 958 Cuts at: 5377 442 Size: 1753 Fragments arranged by size: 1869 1753 1133 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6688 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2066 2338 3116 3221 3575 3718 5348 1239 Size: 827 272 778 105 354 143 1630 2579 Fragments arranged by size: 2579 1630 827 778 354 272 143 105 Bpu10I CC'TnA_GC Cuts at: 3368 3753 3368 Size: 385 6303 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2188 3403 4813 5678 5976 2188 Size: 1215 1410 865 298 2900 Fragments arranged by size: 2900 1410 1215 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2724 5351 882 Size: 617 1225 2627 2219 Fragments arranged by size: 2627 2219 1225 617 BsaAI yAC'GTr Cuts at: 493 1204 2284 3985 4479 493 Size: 711 1080 1701 494 2702 Fragments arranged by size: 2702 1701 1080 711 494 BsaBI GATnn'nnATC Cuts at: 6682 6682 Size: 6688 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2681 2868 3155 3725 Size: 53 83 186 936 1148 187 287 570 Cuts at: 3725 3890 4495 4877 5512 275 Size: 165 605 382 635 1451 Fragments arranged by size: 1451 1148 936 635 605 570 382 287 187 186 165 83 53 BsaWI w'CCGG_w Cuts at: 5121 5519 5793 5940 6351 5121 Size: 398 274 147 411 5458 Fragments arranged by size: 5458 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2738 2768 3280 3310 1178 Size: 30 165 30 1335 30 512 30 4556 Fragments arranged by size: 4556 1335 512 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2316 2586 2689 2759 3244 3568 3572 3894 Size: 1130 270 103 70 485 324 4 322 Cuts at: 3894 1186 Size: 3980 Fragments arranged by size: 3980 1130 485 324 322 270 103 70 4 BseYI C'CCAG_C Cuts at: 2462 5891 6387 2462 Size: 3429 496 2763 Fragments arranged by size: 3429 2763 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2369 1163 Size: 573 633 5482 Fragments arranged by size: 5482 633 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3586 4138 4899 5927 1540 Size: 275 186 1585 552 761 1028 2301 Fragments arranged by size: 2301 1585 1028 761 552 275 186 BsiHKAI G_wGCw'C Cuts at: 2480 2636 3428 4749 4834 5905 2480 Size: 156 792 1321 85 1071 3263 Fragments arranged by size: 3263 1321 1071 792 156 85 BsmI GAATG_Cn' Cuts at: 2140 3403 4199 4292 2140 Size: 1263 796 93 4536 Fragments arranged by size: 4536 1263 796 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2402 2480 2636 2673 2751 3428 3551 Size: 399 612 78 156 37 78 677 123 Cuts at: 3551 3821 4007 4749 4834 5905 1391 Size: 270 186 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 677 612 399 270 186 156 123 85 78 78 37 BspEI T'CCGG_A Cuts at: 6351 6351 Size: 6688 BspHI T'CATG_A Cuts at: 2310 4577 6307 2310 Size: 2267 1730 2691 Fragments arranged by size: 2691 2267 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3015 3238 3948 4043 844 Size: 2171 223 710 95 3489 Fragments arranged by size: 3489 2171 710 223 95 BsrBI CCG'CTC Cuts at: 1394 1823 2520 4575 1394 Size: 429 697 2055 3507 Fragments arranged by size: 3507 2055 697 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3055 5183 5357 65 Size: 1183 1807 2128 174 1396 Fragments arranged by size: 2128 1807 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3615 3876 5332 5519 1700 Size: 110 1805 261 1456 187 2869 Fragments arranged by size: 2869 1805 1456 261 187 110 BsrGI T'GTAC_A Cuts at: 96 2754 96 Size: 2658 4030 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6688 BssSI C'ACGA_G Cuts at: 3544 4748 5760 3544 Size: 1204 1012 4472 Fragments arranged by size: 4472 1204 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6688 BstBI TT'CG_AA Cuts at: 2007 5492 2007 Size: 3485 3203 BstEII G'GTnAC_C Cuts at: 1184 3787 1184 Size: 2603 4085 BstYI r'GATC_y Cuts at: 1972 4018 4385 4770 4787 6239 6325 6337 6683 Size: 2046 367 385 17 1452 86 12 346 Cuts at: 6683 1972 Size: 1977 Fragments arranged by size: 2046 1977 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2055 2328 3923 2055 Size: 273 1595 4820 Fragments arranged by size: 4820 1595 273 BtgI C'CryG_G Cuts at: 513 1065 2690 2724 2919 3216 3301 3492 3718 Size: 552 1625 34 195 297 85 191 226 Cuts at: 3718 513 Size: 3483 Fragments arranged by size: 3483 1625 552 297 226 195 191 85 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2586 487 Size: 683 56 1360 4589 Fragments arranged by size: 4589 1360 683 56 BtsI GCAGTG_nn' Cuts at: 2502 2912 3940 4204 4998 5018 2502 Size: 410 1028 264 794 20 4172 Fragments arranged by size: 4172 1028 794 410 264 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6688 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6653 DraI TTT'AAA Cuts at: 3992 3992 Size: 6688 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2545 5695 809 Size: 507 1229 3150 1802 Fragments arranged by size: 3150 1802 1229 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3187 3583 3613 Size: 54 1425 50 275 18 1357 396 30 Cuts at: 3613 4135 5024 6653 8 Size: 522 889 1629 43 Fragments arranged by size: 1629 1425 1357 889 522 396 275 54 50 43 30 18 EagI C'GGCC_G Cuts at: 1537 1812 3583 4135 1537 Size: 275 1771 552 4090 Fragments arranged by size: 4090 1771 552 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2736 3578 4618 1353 Size: 1383 842 1040 3423 Fragments arranged by size: 3423 1383 1040 842 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2982 5275 5649 5795 387 Size: 1064 138 1393 2293 374 146 1280 Fragments arranged by size: 2293 1393 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3426 3426 Size: 6688 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2066 2338 2562 2773 3116 3221 3417 Size: 393 434 272 224 211 343 105 196 Cuts at: 3417 3575 3718 4751 5348 6135 6403 1239 Size: 158 143 1033 597 787 268 1524 Fragments arranged by size: 1524 1033 787 597 434 393 343 272 268 224 211 196 158 143 105 EcoO109I rG'GnC_Cy Cuts at: 2458 2670 3227 3354 2458 Size: 212 557 127 5792 Fragments arranged by size: 5792 557 212 127 EcoRI G'AATT_C Cuts at: 3997 4375 3997 Size: 378 6310 EcoRV GAT'ATC Cuts at: 2028 2028 Size: 6688 FspI TGC'GCA Cuts at: 3599 5194 3599 Size: 1595 5093 HaeII r_GCGC'y Cuts at: 1678 3622 5835 1678 Size: 1944 2213 2531 Fragments arranged by size: 2531 2213 1944 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2035 2067 2581 2613 3729 Size: 32 135 32 463 32 514 32 1116 Cuts at: 3729 3761 5329 5361 5403 5435 1373 Size: 32 1568 32 42 32 2626 Fragments arranged by size: 2626 1568 1116 514 463 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3487 3797 3888 4032 4278 669 Size: 988 281 1549 310 91 144 246 3079 Fragments arranged by size: 3079 1549 988 310 281 246 144 91 HindIII A'AGCT_T Cuts at: 748 3107 748 Size: 2359 4329 HpaI GTT'AAC Cuts at: 4278 4278 Size: 6688 KpnI G_GTAC'C Cuts at: 3959 4013 3959 Size: 54 6634 MfeI C'AATT_G Cuts at: 4287 4287 Size: 6688 MluI A'CGCG_T Cuts at: 6564 6564 Size: 6688 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3096 3675 5802 5986 1305 Size: 1791 579 2127 184 2007 Fragments arranged by size: 2127 2007 1791 579 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4866 Fragments arranged by size: 4866 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2315 2571 2762 4646 5005 5164 518 Size: 648 1149 256 191 1884 359 159 2042 Fragments arranged by size: 2042 1884 1149 648 359 256 191 159 MspA1I CmG'CkG Cuts at: 1606 2418 2629 2692 2726 2895 3028 3218 3494 Size: 812 211 63 34 169 133 190 276 Cuts at: 3494 3553 4104 4781 5929 6174 1606 Size: 59 551 677 1148 245 2120 Fragments arranged by size: 2120 1148 812 677 551 276 245 211 190 169 133 63 59 34 NaeI GCC'GGC Cuts at: 3617 3617 Size: 6688 NcoI C'CATG_G Cuts at: 513 1065 3301 3718 513 Size: 552 2236 417 3483 Fragments arranged by size: 3483 2236 552 417 NdeI CA'TA_TG Cuts at: 387 387 Size: 6688 NgoMIV G'CCGG_C Cuts at: 3615 3615 Size: 6688 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6688 NotI GC'GGCC_GC Cuts at: 4135 4135 Size: 6688 NspI r_CATG'y Cuts at: 1132 1229 4046 5591 1132 Size: 97 2817 1545 2229 Fragments arranged by size: 2817 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3223 1348 Size: 1875 4813 PciI A'CATG_T Cuts at: 5587 5587 Size: 6688 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6688 PfoI T'CCnGG_A Cuts at: 3533 3533 Size: 6688 PmeI GTTT'AAAC Cuts at: 3992 3992 Size: 6688 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3280 3312 4757 4789 6270 6302 3280 Size: 32 1445 32 1481 32 3666 Fragments arranged by size: 3666 1481 1445 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2458 3227 3354 2458 Size: 769 127 5792 Fragments arranged by size: 5792 769 127 PshAI GACnn'nnGTC Cuts at: 1613 3460 1613 Size: 1847 4841 PsiI TTA'TAA Cuts at: 4258 4258 Size: 6688 PspOMI G'GGCC_C Cuts at: 2669 2669 Size: 6688 PstI C_TGCA'G Cuts at: 830 2509 3637 4040 830 Size: 1679 1128 403 3478 Fragments arranged by size: 3478 1679 1128 403 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6688 PvuII CAG'CTG Cuts at: 2418 2629 2895 2418 Size: 211 266 6211 Fragments arranged by size: 6211 266 211 SacI G_AGCT'C Cuts at: 3428 3428 Size: 6688 SacII CC_GC'GG Cuts at: 2693 2727 3219 3495 2693 Size: 34 492 276 5886 Fragments arranged by size: 5886 492 276 34 SalI G'TCGA_C Cuts at: 1936 3795 3886 4030 1936 Size: 1859 91 144 4594 Fragments arranged by size: 4594 1859 144 91 SanDI GG'GwC_CC Cuts at: 2458 2458 Size: 6688 SbfI CC_TGCA'GG Cuts at: 4040 4040 Size: 6688 ScaI AGT'ACT Cuts at: 4936 6648 4936 Size: 1712 4976 SfcI C'TryA_G Cuts at: 826 1045 2505 3633 4036 5171 5852 6043 826 Size: 219 1460 1128 403 1135 681 191 1471 Fragments arranged by size: 1471 1460 1135 1128 681 403 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6688 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6688 SmaI CCC'GGG Cuts at: 1872 3519 4015 1872 Size: 1647 496 4545 Fragments arranged by size: 4545 1647 496 SmlI C'TyrA_G Cuts at: 820 1017 1943 2151 2167 3418 4792 5693 5955 Size: 197 926 208 16 1251 1374 901 262 Cuts at: 5955 820 Size: 1553 Fragments arranged by size: 1553 1374 1251 926 901 262 208 197 16 SnaBI TAC'GTA Cuts at: 493 3985 4479 493 Size: 3492 494 2702 Fragments arranged by size: 3492 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6688 SphI G_CATG'C Cuts at: 1132 4046 1132 Size: 2914 3774 SspI AAT'ATT Cuts at: 5 52 2579 4612 5 Size: 47 2527 2033 2081 Fragments arranged by size: 2527 2081 2033 47 StuI AGG'CCT Cuts at: 1517 2111 2948 3382 1517 Size: 594 837 434 4823 Fragments arranged by size: 4823 837 594 434 StyI C'CwwG_G Cuts at: 513 1065 2943 2967 3301 3718 4127 513 Size: 552 1878 24 334 417 409 3074 Fragments arranged by size: 3074 1878 552 417 409 334 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2458 3556 4715 4732 4885 5070 5515 Size: 1255 878 1098 1159 17 153 185 445 Cuts at: 5515 325 Size: 1498 Fragments arranged by size: 1498 1255 1159 1098 878 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2085 2754 4934 Size: 274 80 33 51 1443 108 669 2180 Cuts at: 4934 6646 96 Size: 1712 138 Fragments arranged by size: 2180 1712 1443 669 274 138 108 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2268 2300 3094 3126 6648 6680 2268 Size: 32 794 32 3522 32 2276 Fragments arranged by size: 3522 2276 794 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6688 XbaI T'CTAG_A Cuts at: 4024 4024 Size: 6688 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3299 3550 3299 Size: 251 6437 XhoI C'TCGA_G Cuts at: 1943 2151 1943 Size: 208 6480 XmaI C'CCGG_G Cuts at: 1870 3517 4013 1870 Size: 1647 496 4545 Fragments arranged by size: 4545 1647 496 XmnI GAAnn'nnTTC Cuts at: 4817 4817 Size: 6688 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2682 3891 4496 276 Size: 53 83 186 936 1148 1209 605 2468 Fragments arranged by size: 2468 1209 1148 936 605 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SapI SexAI SfiI SfoI SrfI SwaI