Restriction map for
pFN21ASDA1479
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# NO CUT ENZYMES ( 105 enzymes) #
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AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AviII BbeI BbrPI
BbvCI BcgI BciVI BfrI BglII
BlpI Bpu1102I BsaAI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI Bsp1407I
BspEI BspHI BsrBI BsrGI BssHII
BstBI BstEII BstPI Bsu36I CelII
CfoI CpoI DraI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV FseI FspI HaeII
HhaI HinP1I KasI KspI MfeI
MluI MroI MunI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII ScaI SexAI
SfiI SfoI SfuI SmaI SmlI
SnaBI SpeI SphI SspBI SwaI
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 409 GGTACC G^GTACC
AccII 760 CGCG CG^CG
Asp718I 409 GGTACC G^GTACC
AspI 2946 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1244 CTCGGG C^[CT]CG[GA]G
AvrII 2926 CCTAGG C^CTAGG
BanI 409 GGTACC G^G[CT][GA]CC
BanII 1225 GAGCCC G[GA]GC[CT]^C
BclI 2382 TGATCA T^GATCA
BlnI 2926 CCTAGG C^CTAGG
BmyI 1225 GAGCCC G[AGT]GC[ACT]^C
BsaBI 1477 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1969 GGTCTC GGTCTC(1/5)
BsaWI 123 TCCGGT [AT]^CCGG[AT]
BsgI 1428 GTGCAG GTGCAG(16/14)
BsoBI 1244 CTCGGG C^[CT]CG[GA]G
Bsp1286I 1225 GAGCCC G[AGT]GC[ACT]^C
BspLU11I 1877 ACATGT A^CATGT
BsrFI 2420 ACCGGC [GA]^CCGG[CT]
BssSI 1583 CTCGTG CACGAG(-5/-1)
Bst1107I 1861 GTATAC GTA^TAC
BstAPI 1840 GCATTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 760 CGCG CG^CG
BstZ17I 1861 GTATAC GTA^TAC
Cfr10I 2420 ACCGGC [GA]^CCGG[CT]
EcoNI 2717 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1711 GAATTC G^AATTC
EcoT22I 416 ATGCAT ATGCA^T
FbaI 2382 TGATCA T^GATCA
HpaI 1650 GTTAAC GTT^AAC
KpnI 409 GGTACC GGTAC^C
MamI 1477 GATAAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 760 CGCG CG^CG
NdeI 771 CATATG CA^TATG
NsiI 416 ATGCAT ATGCA^T
NspI 1877 ACATGT [GA]CATG^[CT]
PflFI 2946 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1242 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 178 CAGCTG CAG^CTG
SalI 97 GTCGAC G^TCGAC
SbfI 2166 CCTGCAGG CCTGCA^GG
SgrAI 2419 CACCGGCG C[GA]^CCGG[CT]G
Sse8387I 2166 CCTGCAGG CCTGCA^GG
SspI 2005 AATATT AAT^ATT
StuI 1096 AGGCCT AGG^CCT
Tth111I 2946 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcyI 137 GACGCC G[GA]^CG[CT]C
2946 GACGCC
AflIII 1877 ACATGT A^C[GA][CT]GT
2327 ACACGT
BalI 575 TGGCCA TGG^CCA
2202 TGGCCA
BamHI 1615 GGATCC G^GATCC
2541 GGATCC
BcnI 1155 CCCGG CC^[GC]GG
2586 CCGGG
BglI 140 GCCCTTCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
556 GCCACAGTGGC
BsaHI 137 GACGCC G[GA]^CG[CT]C
2946 GACGCC
BspDI 1147 ATCGAT AT^CGAT
2553 ATCGAT
BspMI 2033 ACCTGC ACCTGC(4/8)
1849 GCAGGT
ClaI 1147 ATCGAT AT^CGAT
2553 ATCGAT
DraII 803 GGGTCCT [GA]G^G[ACGT]CC[CT]
2564 AGGTCCC
DrdI 1450 GACAAAAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1978 GACAGCCCTGTC
EcoO109I 803 GGGTCCT [GA]G^G[ACGT]CC[CT]
2564 AGGTCCC
HgaI 137 GACGC GACGC(5/10)
2946 GACGC
Hin1I 137 GACGCC G[GA]^CG[CT]C
2946 GACGCC
HincII 97 GTCGAC GT[CT]^[GA]AC
1650 GTTAAC
HindII 97 GTCGAC GT[CT]^[GA]AC
1650 GTTAAC
HindIII 1130 AAGCTT A^AGCTT
2368 AAGCTT
MaeII 2329 ACGT A^CGT
2966 ACGT
MluNI 575 TGGCCA TGG^CCA
2202 TGGCCA
MscI 575 TGGCCA TGG^CCA
2202 TGGCCA
MspA1I 178 CAGCTG C[AC]G^C[GT]G
799 CAGCGG
NciI 1155 CCCGG CC^[GC]GG
2586 CCGGG
PflMI 1093 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2059 CCACCCAATGG
PpuMI 803 GGGTCCT [GA]G^G[AT]CC[CT]
2564 AGGTCCC
SapI 2178 GAAGAGC GCTCTTC(1/4)
2122 GCTCTTC
Tsp45I 1586 GTGAC ^GT[GC]AC
1630 GTGAC
Van91I 1093 CCAAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2059 CCACCCAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BbsI
BbvI BfaI BpmI BpuAI BsaJI
BsiYI BslI BsmAI BsmFI BsmI
BsrDI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DsaI EaeI EarI EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XcmI XhoII