(Circular) (Six-base) MAPSORT of: pFN21ASDA1438.seq Check: 8709 from: 1 to: 7522 pFN21ASDA1438 7522 bp With 182 enzymes: SgfI * January 16, 2009 15:31 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5332 278 Size: 53 83 186 936 3796 2468 Fragments arranged by size: 3796 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4865 1937 Size: 2928 4594 Acc65I G'GTAC_C Cuts at: 4843 4843 Size: 7522 AclI AA'CG_TT Cuts at: 1563 5649 6022 1563 Size: 4086 373 3063 Fragments arranged by size: 4086 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2219 2225 2327 2729 3107 3119 4205 5585 Size: 587 6 102 402 378 12 1086 1380 Cuts at: 5585 6969 7237 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 1380 1086 587 402 378 268 102 12 6 AfeI AGC'GCT Cuts at: 3401 3401 Size: 7522 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7325 AflIII A'CryG_T Cuts at: 1203 6421 7398 1203 Size: 5218 977 1327 Fragments arranged by size: 5218 1327 977 AgeI A'CCGG_T Cuts at: 6353 6353 Size: 7522 AhdI GACnn_n'nnGTC Cuts at: 6251 6251 Size: 7522 AleI CACnn'nnGTG Cuts at: 1166 3743 1166 Size: 2577 4945 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4508 1184 Size: 3324 4198 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2098 2130 3749 3781 2098 Size: 32 1619 32 5839 Fragments arranged by size: 5839 1619 32 32 AlwNI CAG_nnn'CTG Cuts at: 3097 3601 3661 3793 4115 4564 5265 6837 3097 Size: 504 60 132 322 449 701 1572 3782 Fragments arranged by size: 3782 1572 701 504 449 322 132 60 ApaI G_GGCC'C Cuts at: 2997 3530 3800 3876 3962 2997 Size: 533 270 76 86 6557 Fragments arranged by size: 6557 533 270 86 76 ApaLI G'TGCA_C Cuts at: 5579 6735 5579 Size: 1156 6366 ApoI r'AATT_y Cuts at: 1493 4302 4831 5057 5209 7318 1493 Size: 2809 529 226 152 2109 1697 Fragments arranged by size: 2809 2109 1697 529 226 152 AseI AT'TA_AT Cuts at: 160 1032 6076 160 Size: 872 5044 1606 Fragments arranged by size: 5044 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7522 AvaI C'yCGr_G Cuts at: 1870 1943 2396 2451 2980 3121 3437 3531 3868 Size: 73 453 55 529 141 316 94 337 Cuts at: 3868 3917 4847 1870 Size: 49 930 4545 Fragments arranged by size: 4545 930 529 453 337 316 141 94 73 55 49 BamHI G'GATC_C Cuts at: 4852 5219 4852 Size: 367 7155 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2776 2984 3125 3737 4843 Size: 325 217 627 989 208 141 612 1106 Cuts at: 4843 6298 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1106 989 627 612 325 217 208 141 BanII G_rGCy'C Cuts at: 1391 2997 3049 3193 3368 3530 3557 3706 3800 Size: 1606 52 144 175 162 27 149 94 Cuts at: 3800 3876 3918 3962 4079 4413 4530 4638 4841 Size: 76 42 44 117 334 117 108 203 Cuts at: 4841 1391 Size: 4072 Fragments arranged by size: 4072 1606 334 203 175 162 149 144 117 117 108 94 76 52 44 42 27 BbeI G_GCGC'C Cuts at: 2988 2988 Size: 7522 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4367 928 Size: 971 2468 4083 Fragments arranged by size: 4083 2468 971 BbvCI CC'TCA_GC Cuts at: 3309 4736 3309 Size: 1427 6095 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2387 2421 5713 5747 1903 Size: 34 450 34 3292 34 3678 Fragments arranged by size: 3678 3292 450 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5414 6624 5414 Size: 1210 6312 BclI T'GATC_A Cuts at: 1548 1548 Size: 7522 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3001 3053 3197 Size: 107 122 71 631 605 1329 52 144 Cuts at: 3197 3287 3329 6133 136 Size: 90 42 2804 1525 Fragments arranged by size: 2804 1525 1329 631 605 144 122 107 90 71 52 42 BglII A'GATC_T Cuts at: 7517 7517 Size: 7522 BlpI GC'TnA_GC Cuts at: 2172 3266 3410 3779 4407 4565 4721 4939 2172 Size: 1094 144 369 628 158 156 218 4755 Fragments arranged by size: 4755 1094 628 369 218 158 156 144 Bme1580I G_kGCm'C Cuts at: 1790 2779 2997 3128 3530 3800 3876 3962 5583 Size: 989 218 131 402 270 76 86 1621 Cuts at: 5583 6739 1790 Size: 1156 2573 Fragments arranged by size: 2573 1621 1156 989 402 270 218 131 86 76 BmgBI CAC'GTC Cuts at: 1318 3011 1318 Size: 1693 5829 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2936 3931 4810 Size: 416 53 83 399 24 1519 995 879 Cuts at: 4810 5253 6211 442 Size: 443 958 1753 Fragments arranged by size: 1753 1519 995 958 879 443 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7522 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2465 2816 2959 3288 3707 3751 4092 Size: 929 297 351 143 329 419 44 341 Cuts at: 4092 4160 4419 4484 4496 4715 6182 1239 Size: 68 259 65 12 219 1467 2579 Fragments arranged by size: 2579 1467 929 419 351 341 329 297 259 219 143 68 65 44 12 Bpu10I CC'TnA_GC Cuts at: 3309 3551 3912 4632 4736 3309 Size: 242 361 720 104 6095 Fragments arranged by size: 6095 720 361 242 104 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2714 3659 4237 5647 6512 6810 2714 Size: 945 578 1410 865 298 3426 Fragments arranged by size: 3426 1410 945 865 578 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2156 6185 882 Size: 617 657 4029 2219 Fragments arranged by size: 4029 2219 657 617 BsaAI yAC'GTr Cuts at: 493 1204 4819 5313 493 Size: 711 3615 494 2702 Fragments arranged by size: 3615 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7516 7516 Size: 7522 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2985 4459 5329 5711 Size: 53 83 186 936 1452 1474 870 382 Cuts at: 5711 6346 275 Size: 635 1451 Fragments arranged by size: 1474 1452 1451 936 870 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5955 6353 6627 6774 7185 5955 Size: 398 274 147 411 6292 Fragments arranged by size: 6292 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3086 3116 3749 3779 4434 Size: 30 165 30 1683 30 633 30 655 Cuts at: 4434 4464 4688 4718 1178 Size: 30 224 30 3982 Fragments arranged by size: 3982 1683 655 633 224 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2553 3536 4422 1186 Size: 1367 983 886 4286 Fragments arranged by size: 4286 1367 983 886 BseYI C'CCAG_C Cuts at: 2591 3253 3705 3818 3832 3962 4080 4095 4637 Size: 662 452 113 14 130 118 15 542 Cuts at: 4637 4751 6725 7221 2591 Size: 114 1974 496 2892 Fragments arranged by size: 2892 1974 662 542 496 452 130 118 114 113 15 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3206 4781 1163 Size: 573 1470 1575 3904 Fragments arranged by size: 3904 1575 1470 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2123 4972 5733 6761 1540 Size: 275 186 122 2849 761 1028 2301 Fragments arranged by size: 2849 2301 1028 761 275 186 122 BsiHKAI G_wGCw'C Cuts at: 3272 4146 4413 5583 5668 6739 3272 Size: 874 267 1170 85 1071 4055 Fragments arranged by size: 4055 1170 1071 874 267 85 BsmI GAATG_Cn' Cuts at: 4051 5033 5126 4051 Size: 982 93 6447 Fragments arranged by size: 6447 982 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2779 2997 3049 3128 3193 3272 3368 Size: 399 989 218 52 79 65 79 96 Cuts at: 3368 3530 3557 3706 3800 3876 3918 3962 4079 Size: 162 27 149 94 76 42 44 117 Cuts at: 4079 4146 4413 4530 4638 4841 5583 5668 6739 Size: 67 267 117 108 203 742 85 1071 Cuts at: 6739 1391 Size: 2174 Fragments arranged by size: 2174 1071 989 742 399 267 218 203 162 149 117 117 108 96 94 85 79 79 76 67 65 52 44 42 27 BspEI T'CCGG_A Cuts at: 7185 7185 Size: 7522 BspHI T'CATG_A Cuts at: 2053 5411 7141 2053 Size: 3358 1730 2434 Fragments arranged by size: 3358 2434 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4877 844 Size: 4033 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2077 3670 3803 4296 5409 1394 Size: 429 254 1593 133 493 1113 3507 Fragments arranged by size: 3507 1593 1113 493 429 254 133 BsrDI GCAATG_nn' Cuts at: 65 1248 6017 6191 65 Size: 1183 4769 174 1396 Fragments arranged by size: 4769 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3052 3922 6166 6353 1700 Size: 110 1242 870 2244 187 2869 Fragments arranged by size: 2869 2244 1242 870 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7522 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7522 BssSI C'ACGA_G Cuts at: 3512 5582 6594 3512 Size: 2070 1012 4440 Fragments arranged by size: 4440 2070 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4150 794 Size: 3356 4166 BstBI TT'CG_AA Cuts at: 2007 6326 2007 Size: 4319 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7522 BstXI CCAn_nnnn'nTGG Cuts at: 2269 2323 4654 2269 Size: 54 2331 5137 Fragments arranged by size: 5137 2331 54 BstYI r'GATC_y Cuts at: 1972 2805 2841 3507 4852 5219 5604 5621 7073 Size: 833 36 666 1345 367 385 17 1452 Cuts at: 7073 7159 7171 7517 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1345 833 666 385 367 346 86 36 17 12 Bsu36I CC'TnA_GG Cuts at: 3954 3954 Size: 7522 BtgI C'CryG_G Cuts at: 513 1065 2262 2784 3324 3882 4602 4647 513 Size: 552 1197 522 540 558 720 45 3388 Fragments arranged by size: 3388 1197 720 558 552 540 522 45 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2397 3435 487 Size: 683 56 1171 1038 4574 Fragments arranged by size: 4574 1171 1038 683 56 BtsI GCAGTG_nn' Cuts at: 2465 2619 3110 3343 3404 3500 4148 4572 5038 Size: 154 491 233 61 96 648 424 466 Cuts at: 5038 5832 5852 2465 Size: 794 20 4135 Fragments arranged by size: 4135 794 648 491 466 424 233 154 96 61 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7522 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2482 2517 529 Size: 35 1918 35 5534 Fragments arranged by size: 5534 1918 35 35 DraI TTT'AAA Cuts at: 4826 4826 Size: 7522 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6529 809 Size: 507 5213 1802 Fragments arranged by size: 5213 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2120 3054 3321 Size: 54 1425 50 275 18 290 934 267 Cuts at: 3321 3330 4669 4780 4969 5858 7487 8 Size: 9 1339 111 189 889 1629 43 Fragments arranged by size: 1629 1425 1339 934 889 290 275 267 189 111 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2120 4969 1537 Size: 275 308 2849 4090 Fragments arranged by size: 4090 2849 308 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 2201 2365 2831 4763 5452 1353 Size: 801 47 164 466 1932 689 3423 Fragments arranged by size: 3423 1932 801 689 466 164 47 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3584 6109 6483 6629 387 Size: 1064 138 1995 2525 374 146 1280 Fragments arranged by size: 2525 1995 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4411 4411 Size: 7522 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2219 2225 2327 2465 2729 2816 Size: 393 536 51 6 102 138 264 87 Cuts at: 2816 2959 3107 3119 3288 3707 3751 4092 4160 Size: 143 148 12 169 419 44 341 68 Cuts at: 4160 4205 4419 4484 4496 4715 5585 6182 6969 Size: 45 214 65 12 219 870 597 787 Cuts at: 6969 7237 1239 Size: 268 1524 Fragments arranged by size: 1524 870 787 597 536 419 393 341 268 264 219 214 169 148 143 138 102 87 68 65 51 45 44 12 12 6 EcoNI CCTnn'n_nnAGG Cuts at: 3183 3224 3515 3971 4434 3183 Size: 41 291 456 463 6271 Fragments arranged by size: 6271 463 456 291 41 EcoO109I rG'GnC_Cy Cuts at: 2917 2934 2994 3199 3526 3527 3540 3797 3872 Size: 17 60 205 327 1 13 257 75 Cuts at: 3872 3873 3950 3958 3959 4014 4167 4374 2917 Size: 1 77 8 1 55 153 207 6065 Fragments arranged by size: 6065 327 257 207 205 153 77 75 60 55 17 13 8 1 1 1 EcoRI G'AATT_C Cuts at: 4831 5209 4831 Size: 378 7144 FspI TGC'GCA Cuts at: 6028 6028 Size: 7522 HaeII r_GCGC'y Cuts at: 1678 2988 3151 3403 3632 4115 6669 1678 Size: 1310 163 252 229 483 2554 2531 Fragments arranged by size: 2554 2531 1310 483 252 229 163 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 3413 3445 3454 Size: 32 135 32 803 32 1006 32 9 Cuts at: 3454 3486 4313 4337 4345 4369 4688 4720 6163 Size: 32 827 24 8 24 319 32 1443 Cuts at: 6163 6195 6237 6269 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1443 1006 827 803 319 135 42 32 32 32 32 32 32 32 32 24 24 9 8 HincII GTy'rAC Cuts at: 669 1657 1938 4866 5112 669 Size: 988 281 2928 246 3079 Fragments arranged by size: 3079 2928 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7522 HpaI GTT'AAC Cuts at: 5112 5112 Size: 7522 KasI G'GCGC_C Cuts at: 2984 2984 Size: 7522 KpnI G_GTAC'C Cuts at: 4847 4847 Size: 7522 MfeI C'AATT_G Cuts at: 5121 5121 Size: 7522 MluI A'CGCG_T Cuts at: 7398 7398 Size: 7522 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6636 6820 1305 Size: 5331 184 2007 Fragments arranged by size: 5331 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3323 3332 4671 4782 10 Size: 54 1425 343 1491 9 1339 111 2750 Fragments arranged by size: 2750 1491 1425 1339 343 111 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2321 3074 3743 5480 5839 5998 518 Size: 648 1155 753 669 1737 359 159 2042 Fragments arranged by size: 2042 1737 1155 753 669 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2197 2836 2931 3257 3308 3481 3661 3685 Size: 591 639 95 326 51 173 180 24 Cuts at: 3685 3771 3778 4795 4938 5615 6763 7008 1606 Size: 86 7 1017 143 677 1148 245 2120 Fragments arranged by size: 2120 1148 1017 677 639 591 326 245 180 173 143 95 86 51 24 7 NaeI GCC'GGC Cuts at: 3054 3054 Size: 7522 NarI GG'CG_CC Cuts at: 2985 2985 Size: 7522 NcoI C'CATG_G Cuts at: 513 1065 2262 2784 4602 4647 513 Size: 552 1197 522 1818 45 3388 Fragments arranged by size: 3388 1818 1197 552 522 45 NdeI CA'TA_TG Cuts at: 387 387 Size: 7522 NgoMIV G'CCGG_C Cuts at: 3052 3052 Size: 7522 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7522 NotI GC'GGCC_GC Cuts at: 4969 4969 Size: 7522 NspI r_CATG'y Cuts at: 1132 1229 3643 4880 6425 1132 Size: 97 2414 1237 1545 2229 Fragments arranged by size: 2414 2229 1545 1237 97 PasI CC'CwG_GG Cuts at: 1348 2422 2920 2990 3557 3793 1348 Size: 1074 498 70 567 236 5077 Fragments arranged by size: 5077 1074 567 498 236 70 PciI A'CATG_T Cuts at: 6421 6421 Size: 7522 PflMI CCAn_nnn'nTGG Cuts at: 1108 4264 1108 Size: 3156 4366 PfoI T'CCnGG_A Cuts at: 4473 4581 4473 Size: 108 7414 PmeI GTTT'AAAC Cuts at: 4826 4826 Size: 7522 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2454 2486 3008 3040 3749 3781 5591 5623 7104 Size: 32 522 32 709 32 1810 32 1481 Cuts at: 7104 7136 2454 Size: 32 2840 Fragments arranged by size: 2840 1810 1481 709 522 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2934 3540 4014 4167 4374 2934 Size: 606 474 153 207 6082 Fragments arranged by size: 6082 606 474 207 153 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7522 PsiI TTA'TAA Cuts at: 5092 5092 Size: 7522 PspOMI G'GGCC_C Cuts at: 2993 3526 3796 3872 3958 2993 Size: 533 270 76 86 6557 Fragments arranged by size: 6557 533 270 86 76 PstI C_TGCA'G Cuts at: 830 2814 2841 3492 3507 3606 3684 4155 4874 Size: 1984 27 651 15 99 78 471 719 Cuts at: 4874 830 Size: 3478 Fragments arranged by size: 3478 1984 719 651 471 99 78 27 15 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7522 PvuII CAG'CTG Cuts at: 2197 2836 3308 3481 3661 3685 3771 3778 4795 Size: 639 472 173 180 24 86 7 1017 Cuts at: 4795 2197 Size: 4924 Fragments arranged by size: 4924 1017 639 472 180 173 86 24 7 SacI G_AGCT'C Cuts at: 4413 4413 Size: 7522 SalI G'TCGA_C Cuts at: 1936 4864 1936 Size: 2928 4594 SanDI GG'GwC_CC Cuts at: 2934 4014 4167 4374 2934 Size: 1080 153 207 6082 Fragments arranged by size: 6082 1080 207 153 SapI GCTCTTCn'nnn_ Cuts at: 2365 2831 2365 Size: 466 7056 SbfI CC_TGCA'GG Cuts at: 3492 4874 3492 Size: 1382 6140 ScaI AGT'ACT Cuts at: 5770 7482 5770 Size: 1712 5810 SfcI C'TryA_G Cuts at: 826 1045 2810 2837 3488 3503 3602 3680 4151 Size: 219 1765 27 651 15 99 78 471 Cuts at: 4151 4870 6005 6686 6877 826 Size: 719 1135 681 191 1471 Fragments arranged by size: 1765 1471 1135 719 681 651 471 219 191 99 78 27 15 SfiI GGCCn_nnn'nGGCC Cuts at: 3329 3329 Size: 7522 SfoI GGC'GCC Cuts at: 2986 2986 Size: 7522 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7522 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7522 SmaI CCC'GGG Cuts at: 1872 3439 3533 3870 4849 1872 Size: 1567 94 337 979 4545 Fragments arranged by size: 4545 1567 979 337 94 SmlI C'TyrA_G Cuts at: 820 1017 1943 2729 3674 4252 5626 6527 6789 Size: 197 926 786 945 578 1374 901 262 Cuts at: 6789 820 Size: 1553 Fragments arranged by size: 1553 1374 945 926 901 786 578 262 197 SnaBI TAC'GTA Cuts at: 493 4819 5313 493 Size: 4326 494 2702 Fragments arranged by size: 4326 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7522 SphI G_CATG'C Cuts at: 1132 3643 4880 1132 Size: 2511 1237 3774 Fragments arranged by size: 3774 2511 1237 SspI AAT'ATT Cuts at: 5 52 5446 5 Size: 47 5394 2081 Fragments arranged by size: 5394 2081 47 StuI AGG'CCT Cuts at: 1517 2254 3297 3494 1517 Size: 737 1043 197 5545 Fragments arranged by size: 5545 1043 737 197 StyI C'CwwG_G Cuts at: 513 1065 2262 2700 2784 3195 4602 4647 4961 Size: 552 1197 438 84 411 1407 45 314 Cuts at: 4961 513 Size: 3074 Fragments arranged by size: 3074 1407 1197 552 438 411 314 84 45 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2510 3822 4161 4615 5549 5566 5719 Size: 1255 930 1312 339 454 934 17 153 Cuts at: 5719 5904 6349 325 Size: 185 445 1498 Fragments arranged by size: 1498 1312 1255 934 930 454 445 339 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2742 5768 7480 Size: 274 80 33 51 1443 765 3026 1712 Cuts at: 7480 96 Size: 138 Fragments arranged by size: 3026 1712 1443 765 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3492 3524 4434 4466 4790 4822 7482 7514 3492 Size: 32 910 32 324 32 2660 32 3500 Fragments arranged by size: 3500 2660 910 324 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7522 XbaI T'CTAG_A Cuts at: 4858 4858 Size: 7522 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2269 3340 4645 2269 Size: 1071 1305 5146 Fragments arranged by size: 5146 1305 1071 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7522 XmaI C'CCGG_G Cuts at: 1870 3437 3531 3868 4847 1870 Size: 1567 94 337 979 4545 Fragments arranged by size: 4545 1567 979 337 94 XmnI GAAnn'nnTTC Cuts at: 3761 5651 3761 Size: 1890 5632 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5330 276 Size: 53 83 186 936 3796 2468 Fragments arranged by size: 3796 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SrfI SwaI